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Gene information
General information
TC011711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14608118..14612904
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011711
    iBeetle screen iB_09512

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011711 RA
      MFKKNRPKP TLAHTDSVE NNNVVKSKV PPSPVSPIS 
      KASLQFHCQ LAHGSPTVF VSGFSNVKE LYQKIAECF 
      DFPPSEILF CTLNTYKAD MKKLLGGQI GLEDFIFAH 
      RKGRPKEVE LVKSEDALG LTITDNGAG YAFIKRIKE 
      GSVIHNIPH IQVGDHIEK LNGELMVGK RHFEVAKIL 
      KEIPKGETF TIRLVEPLK SGFNSIAPK SAKGGAKKS 
      NYGSGRETL RFKAGGNAE IEEKDDVMD VGIEKINAI 
      LESFLGIND EDLATQIWE VAQGKSNSM DFADAVDNS 
      DLEELGFTD DLVIELWGA ITDARDGRI
    CDS sequence
    • >TC011711 RA
      atgttcaaa aaaaatcgc ccgaagccg acgttggcg 
      cacaccgac tcggtggag aacaataac gtggtgaag 
      agcaaagtg cccccgtcg ccagtgtcg cccatcagc 
      aaggcctcc ttgcagttc cattgccag ctcgcccac 
      ggcagtccc acggtcttc gtctccgga ttctcaaac 
      gtcaaagaa ctctaccag aaaatcgcc gaatgcttc 
      gacttccca ccctcggag attttattc tgtacgtta 
      aatacgtac aaagcggac atgaagaaa ctactaggg 
      ggtcaaatc ggtttagaa gactttatt ttcgcacac 
      agaaaaggt cgccccaag gaggttgaa ctcgtgaaa 
      agcgaagac gctctcggc ctcacaatc acggacaac 
      ggcgccggt tacgcgttc ataaagcgc atcaaagag 
      ggctccgtc atccacaac atcccccac atccaagtg 
      ggcgaccac atagagaag ctcaacggg gagctgatg 
      gtcggcaag cgccacttc gaagtcgcc aaaatcctc 
      aaagaaatc cccaaaggc gagacgttc acgatccga 
      ctggtcgag cccttgaag agcggcttc aacagcata 
      gcccccaag agcgccaaa gggggcgcc aaaaagtcc 
      aactacggc tcgggccgc gagactttg aggttcaag 
      gcgggagga aacgccgaa attgaggag aaggacgat 
      gtgatggac gtggggatc gagaagatc aacgcgatt 
      ttggagagc ttcctgggg attaacgac gaggacttg 
      gccacccag atctgggag gtggcccag gggaagagc 
      aactcgatg gattttgcg gacgctgtg gacaattcg 
      gatttggag gagttgggg ttcactgac gacttggtg 
      attgagctg tggggggcg atcacggac gcccgggac 
      gggaggatt tga
    mRNA sequence
    • >TC011711 RA
      caataacaa aactacctt cccaaccat catcgtcct 
      ctacgtggt tcagtcaac attaaaacg cgctgtaga 
      tatgtaccg aaaaatcgt aatcttgat aacgtcgcc 
      gttatctcg ataagctcc ctcgactat gtcacacat 
      agtttagtt tcggtgcaa ataaagtgt ggaaatgtt 
      caaaaaaaa tcgcccgaa gccgacgtt ggcgcacac 
      cgactcggt ggagaacaa taacgtggt gaagagcaa 
      agtgccccc gtcgccagt gtcgcccat cagcaaggc 
      ctccttgca gttccattg ccagctcgc ccacggcag 
      tcccacggt cttcgtctc cggattctc aaacgtcaa 
      agaactcta ccagaaaat cgccgaatg cttcgactt 
      cccaccctc ggagatttt attctgtac gttaaatac 
      gtacaaagc ggacatgaa gaaactact agggggtca 
      aatcggttt agaagactt tattttcgc acacagaaa 
      aggtcgccc caaggaggt tgaactcgt gaaaagcga 
      agacgctct cggcctcac aatcacgga caacggcgc 
      cggttacgc gttcataaa gcgcatcaa agagggctc 
      cgtcatcca caacatccc ccacatcca agtgggcga 
      ccacataga gaagctcaa cggggagct gatggtcgg 
      caagcgcca cttcgaagt cgccaaaat cctcaaaga 
      aatccccaa aggcgagac gttcacgat ccgactggt 
      cgagccctt gaagagcgg cttcaacag catagcccc 
      caagagcgc caaaggggg cgccaaaaa gtccaacta 
      cggctcggg ccgcgagac tttgaggtt caaggcggg 
      aggaaacgc cgaaattga ggagaagga cgatgtgat 
      ggacgtggg gatcgagaa gatcaacgc gattttgga 
      gagcttcct ggggattaa cgacgagga cttggccac 
      ccagatctg ggaggtggc ccaggggaa gagcaactc 
      gatggattt tgcggacgc tgtggacaa ttcggattt 
      ggaggagtt ggggttcac tgacgactt ggtgattga 
      gctgtgggg ggcgatcac ggacgcccg ggacgggag 
      gatttgagt gccacttaa tttaagccg attagacaa 
      tgaaaattt aattgtaat ttttaaata aagacactt 
      tgagtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY