Status
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Gene information
General information
TC034451
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011714,TC011715
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14581963..14586774
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034451
    iBeetle screen iB_09511

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034451 RA
      MNFALVLFV IFLSKVDSW RWYPSKYRL SGQVRPLFY 
      SIKIRPNLD ERIFSGEVQ IHVRVETTL EFLDFHAAD 
      LTIQSITFD GRNVANCWC NRGQKWVYG FEPNDLIRI 
      FGVVPPGNH LIRVRYSGN FASDNSHGL FLAGFGDNN 
      TVSNHLLGT DFEPTFARK VFPCLDEPG LKAPIKLGV 
      VVPNRTFNA ISNMPVMKI EETKDGVLY KFQTTPPMS 
      TYLLSFVVS KHSYKEFYN KIPYRIYSS DIESDNKTY 
      LLEFASQVI DFYTKYTNQ SYTLPKIDL VEFEREDST 
      ATENWGLIT LKPGLLSSK EDVFDNPQK YAVIAHELA 
      HFWFGNLVT NKWWNDIWL QEGFATFMS IKAEEKILN 
      NSAELLFSS TYLEEVFWA EASNKTTPI VNYEEFPDN 
      IKKNFNDVT YNKGAAVLR MLEMVLGED FRAGVVKYI 
      KDFAFKTAT TNDFLAIFD QVRPGLDLR DFLESYLYQ 
      TGYPLISVE NRQDRYVLT QRNLGHCTE NLKWTIPLT 
      YITDSDRNA TLVWLDRDV EKLEILKKD ESWIKFNHQ 
      QIGYYRVKY SPDLWNNLL NHTEELEDF ERDHLLLEA 
      TSLFDLNVI SCEIPLKFL QKLAPIGDF NTDNAFIVY 
      LKLAKIRKV DETAFEKLK ELFQEVLEQ AGKKHKMRS 
      FDENLLENE EDVERTKQL QDCVEWLNA HPRICDFPA 
      PLRLKIFSS SVEMNLIYV LIGVLFKNV LCFTSYKLE 
      IENFIYARC KNNTDPEIS NEIRKRLND TYNEFHTEL 
      KDDKPNFCS DNKHKLTQK MKEIATDMK SCFSNPEEQ 
      FLPGFITHG FAEFMEFVC ANRTIEIFF SPQGLDCQD 
      ALKKFRSGQ EIRNCEQEL YKTFRRNVL IEEELCQDL 
      QASEKCFVK AINNGCPHF KAFEALATN LFRAAGVEC 
      NQGGVLLAS GAAVATGVV LSLITRA
    CDS sequence
    • >TC034451 RA
      atgaatttc gcactggtg ctgtttgtg atttttctc 
      tccaaagtg gattcgtgg cgttggtac cccagcaag 
      taccgactt tccggacaa gtccgacct ttgttttac 
      tcaatcaag attcgaccg aacttggat gagagaatt 
      ttctccggc gaagtccaa atccatgtc agagttgaa 
      accacgctg gaattcctc gacttccac gccgctgat 
      ttaacaatc cagagtatt acttttgac ggacgaaac 
      gtcgcaaat tgttggtgt aatcgtggg cagaagtgg 
      gtttatggt tttgaaccc aacgatctc atccggatt 
      tttggggtg gtgccgccc ggaaaccac ctgatccgg 
      gttagatat tccgggaat ttcgccagt gataatagc 
      catgggttg tttctggct gggtttggt gacaataac 
      accgtttca aatcatttg ttagggact gattttgaa 
      ccgactttt gcccggaag gtgtttcct tgcttggac 
      gaaccgggg cttaaagcc cccattaag ctgggggtg 
      gtggtaccc aaccgtacc tttaatgcc atttctaac 
      atgccggtc atgaaaatt gaggaaact aaagacggg 
      gttttgtac aagtttcaa acaacccct ccaatgtcc 
      acttatctc ttatcattc gtcgtctca aagcactcc 
      tacaaggag ttttacaac aaaatccct tacagaatc 
      tacagttca gacatcgaa agcgacaac aaaacctat 
      cttcttgag ttcgcaagc caagtcatc gatttctac 
      acaaaatac acaaatcaa agttacact ttaccaaaa 
      attgatttg gtcgaattc gagcgagag gacagcaca 
      gcgaccgaa aactggggc cttatcacc ctcaaaccc 
      ggcctttta tcctcaaaa gaggacgtt tttgacaat 
      cctcaaaaa tacgcagtg atcgcgcac gaactggcc 
      cacttctgg tttggaaat ctcgtaaca aacaagtgg 
      tggaacgac atttggcta caggaaggt ttcgccact 
      tttatgtca atcaaagcc gaggagaaa atcctgaac 
      aacagtgct gagctgttg ttttcgagc acgtatttg 
      gaggaggtt ttctgggcc gaagcgagc aacaaaacc 
      accccgatt gtgaactat gaggagttt ccggacaat 
      ataaaaaaa aatttcaac gatgttacg tacaataaa 
      ggggctgct gttttgcga atgctggaa atggtactt 
      ggggaggat tttcgtgcc ggagttgta aaatatatc 
      aaagatttt gccttcaaa acggccacg actaacgac 
      tttttggcc atttttgac caagtcagg cccgggctc 
      gatttgcgc gattttctg gaaagttat ttgtaccaa 
      actgggtat ccgctcatt tccgtcgaa aacagacaa 
      gacaggtat gttttgacc caacgaaat ttgggccac 
      tgtaccgaa aatttgaag tggacgatt ccgctgact 
      tacattacc gattctgac cgaaacgcc actcttgtc 
      tggctcgat agagatgtg gaaaaattg gagatattg 
      aaaaaagac gaaagttgg atcaaattc aatcaccaa 
      caaatcggt tattaccgc gtcaagtac tcccctgat 
      ttgtggaat aatttacta aatcacact gaggaattg 
      gaagacttc gaacgtgat cacttgctc ctagaagcc 
      acctcttta ttcgatttg aacgtgatt tcgtgtgag 
      attccgttg aagttttta caaaagttg gccccgatt 
      ggtgatttt aacacggat aatgccttc attgtgtat 
      ttaaaacta gccaaaata agaaaagtt gacgaaaca 
      gctttcgaa aaattaaag gaactgttt caggaggtg 
      ttggaacag gcgggaaaa aaacataaa atgaggagt 
      ttcgatgaa aatttgctc gaaaatgag gaagacgta 
      gaaaggacg aaacagtta caggattgt gtcgaatgg 
      ctaaatgcg caccctcga atttgcgac tttccggcg 
      ccgctgagg ctgaaaatt ttcagcagc agcgtcgag 
      atgaatctt atttatgta ctgatcggc gtgcttttc 
      aaaaatgtg ttgtgtttc acgtcgtac aagctcgaa 
      atcgagaac ttcatctac gcccggtgt aagaacaac 
      accgatcct gaaatctcc aacgagatc aggaaacgc 
      ctcaacgac acttacaac gagtttcac accgaatta 
      aaagacgac aaaccgaat ttctgctcc gacaacaag 
      cacaaactc acccagaaa atgaaggag atcgcgacc 
      gacatgaag tcctgtttc tccaacccg gaggaacag 
      tttttaccc ggttttatc actcacggg tttgccgag 
      tttatggag tttgtgtgt gccaaccgg accattgaa 
      atttttttc agtccgcaa ggactcgat tgtcaggat 
      gcgctgaaa aagttccgg agcgggcag gaaatcagg 
      aactgcgag caggaattg tacaaaacg ttccgtagg 
      aatgtccta atcgaagaa gagttgtgt caggatttg 
      caagcgagt gagaagtgt ttcgtcaaa gcgattaat 
      aacgggtgt ccacatttt aaagctttc gaagctctg 
      gccactaat ttgttccgg gcagcaggg gtggagtgc 
      aatcaaggc ggcgttctg ctggctagt ggcgctgcg 
      gtcgcaact ggggtggtt ttgagtcta attactcga 
      gcttaa
    mRNA sequence
    • >TC034451 RA
      ttgtaatca tcataaaaa tgaatttcg cactggtgc 
      tgtttgtga tttttctct ccaaagtgg attcgtggc 
      gttggtacc ccagcaagt accgacttt ccggacaag 
      tccgacctt tgttttact caatcaaga ttcgaccga 
      acttggatg agagaattt tctccggcg aagtccaaa 
      tccatgtca gagttgaaa ccacgctgg aattcctcg 
      acttccacg ccgctgatt taacaatcc agagtatta 
      cttttgacg gacgaaacg tcgcaaatt gttggtgta 
      atcgtgggc agaagtggg tttatggtt ttgaaccca 
      acgatctca tccggattt ttggggtgg tgccgcccg 
      gaaaccacc tgatccggg ttagatatt ccgggaatt 
      tcgccagtg ataatagcc atgggttgt ttctggctg 
      ggtttggtg acaataaca ccgtttcaa atcatttgt 
      tagggactg attttgaac cgacttttg cccggaagg 
      tgtttcctt gcttggacg aaccggggc ttaaagccc 
      ccattaagc tgggggtgg tggtaccca accgtacct 
      ttaatgcca tttctaaca tgccggtca tgaaaattg 
      aggaaacta aagacgggg ttttgtaca agtttcaaa 
      caacccctc caatgtcca cttatctct tatcattcg 
      tcgtctcaa agcactcct acaaggagt tttacaaca 
      aaatccctt acagaatct acagttcag acatcgaaa 
      gcgacaaca aaacctatc ttcttgagt tcgcaagcc 
      aagtcatcg atttctaca caaaataca caaatcaaa 
      gttacactt taccaaaaa ttgatttgg tcgaattcg 
      agcgagagg acagcacag cgaccgaaa actggggcc 
      ttatcaccc tcaaacccg gccttttat cctcaaaag 
      aggacgttt ttgacaatc ctcaaaaat acgcagtga 
      tcgcgcacg aactggccc acttctggt ttggaaatc 
      tcgtaacaa acaagtggt ggaacgaca tttggctac 
      aggaaggtt tcgccactt ttatgtcaa tcaaagccg 
      aggagaaaa tcctgaaca acagtgctg agctgttgt 
      tttcgagca cgtatttgg aggaggttt tctgggccg 
      aagcgagca acaaaacca ccccgattg tgaactatg 
      aggagtttc cggacaata taaaaaaaa atttcaacg 
      atgttacgt acaataaag gggctgctg ttttgcgaa 
      tgctggaaa tggtacttg gggaggatt ttcgtgccg 
      gagttgtaa aatatatca aagattttg ccttcaaaa 
      cggccacga ctaacgact ttttggcca tttttgacc 
      aagtcaggc ccgggctcg atttgcgcg attttctgg 
      aaagttatt tgtaccaaa ctgggtatc cgctcattt 
      ccgtcgaaa acagacaag acaggtatg ttttgaccc 
      aacgaaatt tgggccact gtaccgaaa atttgaagt 
      ggacgattc cgctgactt acattaccg attctgacc 
      gaaacgcca ctcttgtct ggctcgata gagatgtgg 
      aaaaattgg agatattga aaaaagacg aaagttgga 
      tcaaattca atcaccaac aaatcggtt attaccgcg 
      tcaagtact cccctgatt tgtggaata atttactaa 
      atcacactg aggaattgg aagacttcg aacgtgatc 
      acttgctcc tagaagcca cctctttat tcgatttga 
      acgtgattt cgtgtgaga ttccgttga agtttttac 
      aaaagttgg ccccgattg gtgatttta acacggata 
      atgccttca ttgtgtatt taaaactag ccaaaataa 
      gaaaagttg acgaaacag ctttcgaaa aattaaagg 
      aactgtttc aggaggtgt tggaacagg cgggaaaaa 
      aacataaaa tgaggagtt tcgatgaaa atttgctcg 
      aaaatgagg aagacgtag aaaggacga aacagttac 
      aggattgtg tcgaatggc taaatgcgc accctcgaa 
      tttgcgact ttccggcgc cgctgaggc tgaaaattt 
      tcagcagca gcgtcgaga tgaatctta tttatgtac 
      tgatcggcg tgcttttca aaaatgtgt tgtgtttca 
      cgtcgtaca agctcgaaa tcgagaact tcatctacg 
      cccggtgta agaacaaca ccgatcctg aaatctcca 
      acgagatca ggaaacgcc tcaacgaca cttacaacg 
      agtttcaca ccgaattaa aagacgaca aaccgaatt 
      tctgctccg acaacaagc acaaactca cccagaaaa 
      tgaaggaga tcgcgaccg acatgaagt cctgtttct 
      ccaacccgg aggaacagt ttttacccg gttttatca 
      ctcacgggt ttgccgagt ttatggagt ttgtgtgtg 
      ccaaccgga ccattgaaa tttttttca gtccgcaag 
      gactcgatt gtcaggatg cgctgaaaa agttccgga 
      gcgggcagg aaatcagga actgcgagc aggaattgt 
      acaaaacgt tccgtagga atgtcctaa tcgaagaag 
      agttgtgtc aggatttgc aagcgagtg agaagtgtt 
      tcgtcaaag cgattaata acgggtgtc cacatttta 
      aagctttcg aagctctgg ccactaatt tgttccggg 
      cagcagggg tggagtgca atcaaggcg gcgttctgc 
      tggctagtg gcgctgcgg tcgcaactg gggtggttt 
      tgagtctaa ttactcgag cttaattaa ttaatatga 
      cgcatgata cttgtgctc tatttattt ttctgtgta 
      cctatgtac ttacagttt ttgtattta gatgaatat 
      aaagttgtt aaaaccact tgtctttta aataaatta 
      accaatcat ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY