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Gene information
General information
TC012753 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14243057..14247798
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012753
    iBeetle screen iB_09510

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012753 RA
      MVGKIDPEE CLRDSPKFR SLLEEEEAS VDQLEQKLE 
      KILKVCGTM VDSGKTYVA QQSLFANSL WELSAHFRE 
      DSAVLASLN KLIQSLQEM NKFHTILLD QASRTILKN 
      LTSFIKNDI KTVRDYKQH FEKISSEFD NVLVRNSQT 
      PRSKQQEVE EMQNVLLAV RSCFGHQAL DYVNSISLL 
      QTKKRHEIL STLLSYMHA CTAYYHQGS DLCADLEPF 
      FKELADEIA DMREETTTV EKELENRHA SVNNIEIIT 
      PKIGKNLPR MEGYLFKRT SNAFKTWNR RWFYLYDNK 
      LVYRKRSGE EAETVMEDD LRLCTVKPV LDGERRFCF 
      EVLSPSKSH MLQADSKEM YEAWIAALQ KGIGAAFQR 
      IHSIECSNN ASGQSNHSN DEQSGHSVA NYNNNKIKK 
      IRMWEQLLK IPGNNYCCD CGSANPHWA SINLGITLC 
      IECSGVHRS LGVHYSKVR SLTLDDWEP EIIKVMVEL 
      GNTIVNQIY EAQVPDDFV RATPDCPGT IRESWIKAK 
      YVDKKFVKN LPHFDSQQR TSRASLMEI RKWSVRKIR 
      RRPRSCDNA KRRAKTQFK SQEDVSRDR ISETSESSE 
      AETGNRGVL LFGDLDKQP LDGTIDLSS DQESTAGEG 
      EDTVEEEDI DKLHPNLLL YKAAVAHNL PVMCEALAL 
      GADKQWINL EDRGRSPIH QAILSGSVM PCAYLILNG 
      AKINAQDQN GKTPLHLAT QEGHTAQVC LLLKHRADQ 
      HLEDDEGKV PLQMAEQKE HADIVTLLR LGRLNDEMK 
      ESENGLTGD SMFNDVVRD FSQLACSHP ERLQRQKID 
      NK
    CDS sequence
    • >TC012753 RA
      atggtgggt aaaatcgac ccggaagag tgcctgcgt 
      gattccccc aagttcagg agtcttctg gaggaggag 
      gaggccagt gttgatcag ctggaacag aagctggag 
      aagatcctg aaagtatgt gggacgatg gtcgattcg 
      ggcaaaaca tatgtggcc caacaaagt ttgtttgcg 
      aacagtttg tgggagttg agcgcgcat tttcgtgag 
      gattccgct gttctggcc tcgcttaat aagttgatt 
      cagagcttg caagaaatg aacaagttc catactatc 
      cttctggac caagcctca agaaccata cttaagaat 
      ctcacgtcg tttatcaaa aatgacata aaaaccgtt 
      cgcgattat aagcaacac tttgagaaa atctcttcc 
      gaatttgac aacgtttta gtgcgcaat tcgcaaact 
      cccagaagc aaacagcag gaagttgag gaaatgcag 
      aatgtttta ttggcggta cggtcctgt ttcgggcac 
      caggccctc gattacgtc aacagtatt agtttgctt 
      cagacgaaa aaacggcac gagattttg agcacgttg 
      ttatcatat atgcacgcc tgtacggct tattaccac 
      caaggttcc gatctgtgt gccgacttg gagccgttt 
      ttcaaagaa ttggccgat gagatcgcc gatatgcgc 
      gaggaaacc acaaccgtt gaaaaagaa ttagagaac 
      agacacgct tctgttaat aatattgaa ataattaca 
      ccgaaaatc gggaaaaat ttgcctagg atggaaggt 
      tatttgttt aaaaggacc tcgaatgct ttcaagact 
      tggaaccgg cgttggttt tacctctat gacaataaa 
      ttagtttat aggaaacga tctggtgaa gaggctgag 
      actgtgatg gaggacgat ttgcgtctt tgtaccgtt 
      aaaccggtc ctagatggg gagaggcgg ttttgtttt 
      gaggttttg tcaccgtct aagagtcat atgctccaa 
      gccgactct aaggaaatg tacgaagct tggattgct 
      gcgcttcaa aagggcata ggtgcagct tttcaacgc 
      atacatagt attgaatgt agtaataat gccagtggg 
      caatcgaat cattctaac gacgaacaa agcggacat 
      tccgtcgcc aattacaat aataataaa attaagaaa 
      ataagaatg tgggagcaa cttttgaaa atccccggc 
      aacaactac tgttgcgac tgcggcagt gccaacccg 
      cactgggcg agcataaac ctgggcata accctctgc 
      atagaatgc tcgggggtt caccgaagt ctgggcgtc 
      cactacagc aaagtccgc tctctaacc ctcgacgac 
      tgggaaccg gaaatcatc aaagtgatg gtcgaactg 
      ggcaacacc atcgtaaac caaatctac gaggcccaa 
      gttccggac gatttcgtc cgcgccact ccagactgt 
      cccggaaca atccgggag tcgtggatc aaggcgaaa 
      tacgtcgat aaaaaattc gtcaagaat ttgccacat 
      ttcgacagt caacagcgc acctcgcgg gccagtttg 
      atggaaatc cggaagtgg agtgtccgg aaaatccgg 
      cgacggccc cggagctgc gataatgcg aaacgaagg 
      gctaaaaca cagtttaag tcacaggag gatgtttcg 
      agagatcgt atttcggag actagtgag agtagcgaa 
      gcggagacg gggaatcgg ggggttttg ctgtttggc 
      gatttggat aagcagccg ctagatggc acgattgat 
      ttgagtagt gaccaggag tcgacggct ggggagggg 
      gaggacacg gtcgaggag gaagatata gataaactt 
      cacccgaat ttgctgctt tataaagcc gcggttgcg 
      cataatttg ccggttatg tgcgaggct cttgcttta 
      ggggccgat aaacagtgg attaatctc gaggatagg 
      gggcgcagt ccgatacat caagcgatt ttaagtggg 
      tcggtaatg ccttgcgct tacttgatt ctgaacggt 
      gctaaaatc aacgcccag gaccaaaac ggcaaaacg 
      ccgctgcat ctcgcgaca caggaaggg cacacggcc 
      caggtttgt cttttgttg aaacacagg gccgaccag 
      cacctggag gacgacgag gggaaggtc cctttgcag 
      atggcggag cagaaggag cacgcggac attgtcact 
      ctgttgcgc ttagggcgt ctcaacgac gagatgaag 
      gaatccgag aacggatta acaggcgac agtatgttt 
      aacgacgtt gttagggat ttttcgcaa cttgcgtgc 
      tcgcatcct gagagacta caacgacag aaaatcgac 
      aacaaatga
    mRNA sequence
    • >TC012753 RA
      tgctttcgt taacgtttt accctaatt atagtccga 
      taaaccaag ctaaagatg gtttgctcg tgataacaa 
      cgaattaag ctttgaaac gcgtaaaat cgctgctgg 
      ttcgcgggg tcacgttgg cagcccgcg cttttgacg 
      tttgacgtc cacaataat aaagaaagt gggttgtct 
      gagagttga tgactcaaa ataagtgtt atgaccgct 
      taattagtt agtttgtgg ccatggtgg gtaaaatcg 
      acccggaag agtgcctgc gtgattccc ccaagttca 
      ggagtcttc tggaggagg aggaggcca gtgttgatc 
      agctggaac agaagctgg agaagatcc tgaaagtat 
      gtgggacga tggtcgatt cgggcaaaa catatgtgg 
      cccaacaaa gtttgtttg cgaacagtt tgtgggagt 
      tgagcgcgc attttcgtg aggattccg ctgttctgg 
      cctcgctta ataagttga ttcagagct tgcaagaaa 
      tgaacaagt tccatacta tccttctgg accaagcct 
      caagaacca tacttaaga atctcacgt cgtttatca 
      aaaatgaca taaaaaccg ttcgcgatt ataagcaac 
      actttgaga aaatctctt ccgaatttg acaacgttt 
      tagtgcgca attcgcaaa ctcccagaa gcaaacagc 
      aggaagttg aggaaatgc agaatgttt tattggcgg 
      tacggtcct gtttcgggc accaggccc tcgattacg 
      tcaacagta ttagtttgc ttcagacga aaaaacggc 
      acgagattt tgagcacgt tgttatcat atatgcacg 
      cctgtacgg cttattacc accaaggtt ccgatctgt 
      gtgccgact tggagccgt ttttcaaag aattggccg 
      atgagatcg ccgatatgc gcgaggaaa ccacaaccg 
      ttgaaaaag aattagaga acagacacg cttctgtta 
      ataatattg aaataatta caccgaaaa tcgggaaaa 
      atttgccta ggatggaag gttatttgt ttaaaagga 
      cctcgaatg ctttcaaga cttggaacc ggcgttggt 
      tttacctct atgacaata aattagttt ataggaaac 
      gatctggtg aagaggctg agactgtga tggaggacg 
      atttgcgtc tttgtaccg ttaaaccgg tcctagatg 
      gggagaggc ggttttgtt ttgaggttt tgtcaccgt 
      ctaagagtc atatgctcc aagccgact ctaaggaaa 
      tgtacgaag cttggattg ctgcgcttc aaaagggca 
      taggtgcag cttttcaac gcatacata gtattgaat 
      gtagtaata atgccagtg ggcaatcga atcattcta 
      acgacgaac aaagcggac attccgtcg ccaattaca 
      ataataata aaattaaga aaataagaa tgtgggagc 
      aacttttga aaatccccg gcaacaact actgttgcg 
      actgcggca gtgccaacc cgcactggg cgagcataa 
      acctgggca taaccctct gcatagaat gctcggggg 
      ttcaccgaa gtctgggcg tccactaca gcaaagtcc 
      gctctctaa ccctcgacg actgggaac cggaaatca 
      tcaaagtga tggtcgaac tgggcaaca ccatcgtaa 
      accaaatct acgaggccc aagttccgg acgatttcg 
      tccgcgcca ctccagact gtcccggaa caatccggg 
      agtcgtgga tcaaggcga aatacgtcg ataaaaaat 
      tcgtcaaga atttgccac atttcgaca gtcaacagc 
      gcacctcgc gggccagtt tgatggaaa tccggaagt 
      ggagtgtcc ggaaaatcc ggcgacggc cccggagct 
      gcgataatg cgaaacgaa gggctaaaa cacagttta 
      agtcacagg aggatgttt cgagagatc gtatttcgg 
      agactagtg agagtagcg aagcggaga cggggaatc 
      ggggggttt tgctgtttg gcgatttgg ataagcagc 
      cgctagatg gcacgattg atttgagta gtgaccagg 
      agtcgacgg ctggggagg gggaggaca cggtcgagg 
      aggaagata tagataaac ttcacccga atttgctgc 
      tttataaag ccgcggttg cgcataatt tgccggtta 
      tgtgcgagg ctcttgctt taggggccg ataaacagt 
      ggattaatc tcgaggata gggggcgca gtccgatac 
      atcaagcga ttttaagtg ggtcggtaa tgccttgcg 
      cttacttga ttctgaacg gtgctaaaa tcaacgccc 
      aggaccaaa acggcaaaa cgccgctgc atctcgcga 
      cacaggaag ggcacacgg cccaggttt gtcttttgt 
      tgaaacaca gggccgacc agcacctgg aggacgacg 
      aggggaagg tccctttgc agatggcgg agcagaagg 
      agcacgcgg acattgtca ctctgttgc gcttagggc 
      gtctcaacg acgagatga aggaatccg agaacggat 
      taacaggcg acagtatgt ttaacgacg ttgttaggg 
      atttttcgc aacttgcgt gctcgcatc ctgagagac 
      tacaacgac agaaaatcg acaacaaat gatcgaatt 
      tattgcggc gggtttttc gccggtaat tttatcacg 
      tttatactc attccaatt tttttaata atgccttga 
      ggaagtttt tataattgc cttaacatg tgatattag 
      cattttttt acttccgaa atgccaaat tttttatag 
      ctcaatgat gtgctcatg ttttgacag ttgttttat 
      aatgacctc taatgaaca gtagctgta attgtacct 
      acaagccag actttatac tcaaccaag cttgtaggc 
      ggtgtaata aaaactgac ctaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY