Status
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Gene information
General information
TC011734 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14232941..14241335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011734
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09509

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011734 RA
      MATTARFAA GGSSSPPTP TPATSPPAQ TPATQLIVL 
      PASLHQSGT GGAIVNGVP VIDVSALEQ AAESSNEST 
      ATAEQQGDT HDTDNITQS SPHFITVTV ADGDTVASH 
      QSYHIQYVE PEIYASQTN SGQAQMAYP VYTVGESTT 
      LYSAGQGQY YAATNTGSS ATAGTVGYS TTSGHYVVQ 
      QAVDPDTLI ASTRNSPQT TNAEPSYVA TATVHATQS 
      NPVSNEEVG SALGHATRV SPATVQWLL ENYETAEGV 
      SLPRSTLYA HYLRHCAEN KLEPVNAAS FGKLIRSVF 
      LGLRTRRLG TRGNSKYHY YGIRVKPGS SLMNMDESG 
      NRVVLNTSN ASGKAQGTV RPFKRNSDS SDASISVSV 
      LSQHVTYLG DGSNAIPAF PDIHFTAES LPDECGYED 
      VDTLKSIYR EHLEAFLDA ILSLEFTTI ESLWREFWR 
      SQYNNNGDE CEEEKYLSK SKLFCLCSM EAVQQFMKQ 
      VDLVFYQNL IQVLVPDVL KPIPATLTQ SIRNFAKNL 
      ENWLTSAMA GAPPGMLTV KLSAVSAFS SSLRRYTSL 
      NHLAQAARA VLHNSTQIA QMLTDLNRV DFHSVREQA 
      SWVCQCDNE IVARFENDF KLTLQQQNS LEQWASWLK 
      NVVKSALKP YEGKPSFSK AARQFLLKW SFYSSMVIR 
      DLTLRSAAS FGSFHLIRL LYDEYMFYL IEHQVAEAT 
      GVVPIAVIL EGIKQDIMQ NSLSIYGND GVCNGGVSA 
      KLSCVSPSD HLSKRAKIS
    CDS sequence
    • >TC011734 RA
      atggcaact acagctcga tttgcagca ggaggcagc 
      agcagccct cccactcca acgccagcc acttcaccc 
      ccggcacag actccggct acccagcta atcgtactg 
      cctgctagt ttgcaccag tcgggcacc ggaggggct 
      atcgtcaac ggagtgccg gtcatcgac gtctcagca 
      ctggagcag gcggcagaa tcaagcaac gagagcaca 
      gccactgct gagcaacag ggggataca cacgatacg 
      gataatatt acacaaagc tcgccgcat tttatcaca 
      gtcacagtt gccgacggt gacacggtg gcatcgcat 
      caaagctat cacatacag tatgtggaa cccgagatc 
      tacgcgtcc caaacgaac agcggacag gcacaaatg 
      gcgtatccg gtgtacact gtgggcgaa tcaacaact 
      ttgtattcc gccggtcag gggcagtat tacgccgcc 
      accaacacc ggctcctct gccacggca ggcaccgtg 
      ggttacagc accacaagt ggccactac gtggtgcaa 
      caggccgtc gatcccgac actttgatc gcatcgaca 
      cgaaactcc ccacaaaca acaaatgct gaaccgagc 
      tatgtagct acagccacc gtgcatgca actcaaagc 
      aatccagtg agcaatgag gaggtcggg tcggcgttg 
      ggacacgcc acgagggtg tctccggct actgtgcaa 
      tggttgttg gagaattac gaaacggcc gaaggtgtg 
      tccctgccg cggtctacg ctctatgct cattatttg 
      cgccactgt gccgagaat aaattagag ccggtaaac 
      gcggcctcg ttcggtaaa ttaatcaga tcggtgttt 
      ctcgggttg agaacgcga agactcgga actagaggc 
      aactccaag tatcattac tacggaatt cgggtaaaa 
      cccggttct tcattgatg aatatggac gaatcgggg 
      aacagagtc gtactgaat acatcgaac gcgtcggga 
      aaagcccaa ggaacggtt agaccgttt aagagaaat 
      tctgattcg tcggatgcg tcgatttcg gtcagtgtt 
      ttgtcgcaa catgtgact tatctaggg gatggaagt 
      aacgcgatc ccggctttt cccgacata cacttcacg 
      gctgagtcg ctacccgac gagtgtggg tatgaagac 
      gtcgatact ttgaaatcc atatatagg gaacattta 
      gaagccttc ttagatgct atattaagt ttagaattt 
      accacaata gagtcgctc tggagggaa ttctggaga 
      tcacaatat aacaataac ggcgacgaa tgcgaagaa 
      gaaaagtat ctctcgaaa tcgaagttg ttctgtttg 
      tgttcgatg gaggctgtc caacagttt atgaaacag 
      gtcgatctg gtgttttat cagaatttg atccaagtt 
      ttggtacct gacgtactt aagcccata ccggccacc 
      ctgacccag tcgattagg aatttcgcc aaaaatttg 
      gaaaactgg ctgacgagc gcgatggca ggggcgcct 
      cccggcatg ttaacggtg aaactctcg gccgtttcg 
      gccttcagt tcgagcctt cgccgctac acatccctc 
      aaccactta gcccaagcc gctcgtgcc gtactccac 
      aacagcact caaatcgcc caaatgttg accgatttg 
      aaccgcgtt gactttcac agcgtgcgt gagcaagcc 
      tcctgggtc tgtcagtgc gacaacgag atcgtggct 
      cgcttcgaa aacgatttc aagctcact ttgcagcag 
      caaaactcg ctcgaacag tgggcctca tggctaaag 
      aacgtcgtt aagagcgcg ctcaagccc tacgagggc 
      aagccgagc ttctcgaag gcggcgagg cagttcctc 
      ctcaagtgg agcttctac agctcgatg gtcattcgg 
      gacttgact ctgcggtcg gctgcgagt ttcgggtcg 
      ttccacctg atccgcctg ctgtacgac gagtacatg 
      ttttatctg attgagcac caggtcgcc gaagctacg 
      ggtgtggtt cctattgct gttatcctg gagggaatc 
      aaacaggac ataatgcaa aacagtttg tcgatttat 
      ggcaacgac ggggtttgc aatggaggt gtgagcgca 
      aagttgagt tgtgtgagt ccctctgac catctttcg 
      aaaagggcg aaaattagc tag
    mRNA sequence
    • >TC011734 RA
      aatagttgt gaaatcgct tattaatta aaatcgcgc 
      ttatcattg ttgtgataa ggtgtagtt tggcggcga 
      tcgtgaaag atagcgaaa agcgcgtcg taaggtaag 
      ttttcaggt cggtatttg gcgattcca tcgcgatga 
      cgtcacagt gtgttgtac ggggtgttg ttgaaaatg 
      ttgtataaa tcggtgcat atcgtgaaa tagcgtcgt 
      tttgtttgt aaagttttg ttctttttc gtgttggtt 
      attgattga attgcgctt gaagccgcc gcgctagct 
      cgtcttgta aaatcgcag tttcctgca ttaccggcc 
      atggaggcc tcgttgcgt taaataatc aataatata 
      aagtcggtc tgttttaat aataatcgg gccttgagg 
      cgatgatcg gagtgcgtt taggggtcc cccttgggt 
      ggtgagccc ccttggccc gtttcggca acccgccag 
      ctgtcaaat cggaggaat ctcattttc gtatccttg 
      gagactgcc ttagcaacc gatacaaca acataacaa 
      cattagaga gagcatcag gagtagcct tggaaacgc 
      agccatggc aactacagc tcgatttgc agcaggagg 
      cagcagcag ccctcccac tccaacgcc agccacttc 
      acccccggc acagactcc ggctaccca gctaatcgt 
      actgcctgc tagtttgca ccagtcggg caccggagg 
      ggctatcgt caacggagt gccggtcat cgacgtctc 
      agcactgga gcaggcggc agaatcaag caacgagag 
      cacagccac tgctgagca acaggggga tacacacga 
      tacggataa tattacaca aagctcgcc gcattttat 
      cacagtcac agttgccga cggtgacac ggtggcatc 
      gcatcaaag ctatcacat acagtatgt ggaacccga 
      gatctacgc gtcccaaac gaacagcgg acaggcaca 
      aatggcgta tccggtgta cactgtggg cgaatcaac 
      aactttgta ttccgccgg tcaggggca gtattacgc 
      cgccaccaa caccggctc ctctgccac ggcaggcac 
      cgtgggtta cagcaccac aagtggcca ctacgtggt 
      gcaacaggc cgtcgatcc cgacacttt gatcgcatc 
      gacacgaaa ctccccaca aacaacaaa tgctgaacc 
      gagctatgt agctacagc caccgtgca tgcaactca 
      aagcaatcc agtgagcaa tgaggaggt cgggtcggc 
      gttgggaca cgccacgag ggtgtctcc ggctactgt 
      gcaatggtt gttggagaa ttacgaaac ggccgaagg 
      tgtgtccct gccgcggtc tacgctcta tgctcatta 
      tttgcgcca ctgtgccga gaataaatt agagccggt 
      aaacgcggc ctcgttcgg taaattaat cagatcggt 
      gtttctcgg gttgagaac gcgaagact cggaactag 
      aggcaactc caagtatca ttactacgg aattcgggt 
      aaaacccgg ttcttcatt gatgaatat ggacgaatc 
      ggggaacag agtcgtact gaatacatc gaacgcgtc 
      gggaaaagc ccaaggaac ggttagacc gtttaagag 
      aaattctga ttcgtcgga tgcgtcgat ttcggtcag 
      tgttttgtc gcaacatgt gacttatct aggggatgg 
      aagtaacgc gatcccggc ttttcccga catacactt 
      cacggctga gtcgctacc cgacgagtg tgggtatga 
      agacgtcga tactttgaa atccatata tagggaaca 
      tttagaagc cttcttaga tgctatatt aagtttaga 
      atttaccac aatagagtc gctctggag ggaattctg 
      gagatcaca atataacaa taacggcga cgaatgcga 
      agaagaaaa gtatctctc gaaatcgaa gttgttctg 
      tttgtgttc gatggaggc tgtccaaca gtttatgaa 
      acaggtcga tctggtgtt ttatcagaa tttgatcca 
      agttttggt acctgacgt acttaagcc cataccggc 
      caccctgac ccagtcgat taggaattt cgccaaaaa 
      tttggaaaa ctggctgac gagcgcgat ggcaggggc 
      gcctcccgg catgttaac ggtgaaact ctcggccgt 
      ttcggcctt cagttcgag ccttcgccg ctacacatc 
      cctcaacca cttagccca agccgctcg tgccgtact 
      ccacaacag cactcaaat cgcccaaat gttgaccga 
      tttgaaccg cgttgactt tcacagcgt gcgtgagca 
      agcctcctg ggtctgtca gtgcgacaa cgagatcgt 
      ggctcgctt cgaaaacga tttcaagct cactttgca 
      gcagcaaaa ctcgctcga acagtgggc ctcatggct 
      aaagaacgt cgttaagag cgcgctcaa gccctacga 
      gggcaagcc gagcttctc gaaggcggc gaggcagtt 
      cctcctcaa gtggagctt ctacagctc gatggtcat 
      tcgggactt gactctgcg gtcggctgc gagtttcgg 
      gtcgttcca cctgatccg cctgctgta cgacgagta 
      catgtttta tctgattga gcaccaggt cgccgaagc 
      tacgggtgt ggttcctat tgctgttat cctggaggg 
      aatcaaaca ggacataat gcaaaacag tttgtcgat 
      ttatggcaa cgacggggt ttgcaatgg aggtgtgag 
      cgcaaagtt gagttgtgt gagtccctc tgaccatct 
      ttcgaaaag ggcgaaaat tagctagct ttcaaaagg 
      ggcgaagtt tatcgtgac atttcaata agttggttc 
      ggttggtta tgttgcggt aaatacggg accattatc 
      ttttcgttt cttcatagt ttctcatta atttatttc 
      acgtattta ggatgtgta atttaaatg tttgaattg 
      tgcaattgg ttatgaagc atgtataag tgccgtcaa 
      atttgtgtg acaataggt gtatatatt atacttata 
      tatgtacat tgccgctaa aggaattct gtaattata 
      agtcactga gatcaagag ttcttagac tgatttcgt 
      tggattttg ttgtgtgta cataattat ttattgttt 
      tttttttcg tttctgtgt gttaataat tgttatttt 
      ttctgcaag tgttttatt tcggttttc ggtgatttg 
      attcgacga gtttattgc tcaaaagtg taatccatc 
      cattaaaat ataatagtt gaacagacg actacgcag 
      ggtatgaat attcctcta agcagtaca agtaagtgt 
      ttgatgtta ttcttgcta aagccccca ttcataaat 
      tgtctcact ggtagtttc accaatcat gcttgaaac 
      aatcgcaat tggctacta cagggtaaa aaatggtct 
      ccattgttt tactactgc tggcgacaa atatgacgg 
      gtcgacttt caaccggtt gcctgatgt acatttatt 
      tgtaaaaaa aagctaatt ttgtatttt ccaaataaa 
      tgtggcttt tatttgacg aatattcgt gtgcttcat 
      cgactatgt gtgattgct ggatgcact ctttcagtt 
      cggtagcag tagggaatt ccatgccaa aacgacact 
      ttgaacccc atctgcgat ttcgccaga tttgagact 
      tcgccggct ttgtttcgt acaacagtg ccaagaaaa 
      tcgccgttt aaacgaaaa gtaatcatg ggcatctca 
      ctcgctatt gctcgtacg tgctttaaa cagtatcaa 
      caaattatt tttcattcc ttaacttgt tttaaaaaa 
      aagcggtga attagggcc attggtctc ctcgaattt 
      caagatcgc agatggggc tcaagaagt ttcgcacgg 
      aactgctca gtagccttc gattttgac tgctactgg 
      tcattgtat ctagagaga aagatctgt gtatttttg 
      tagatgtca gtataagaa actgcttta acccacttt 
      tctgttggc cgctttgcg aaagtcggt tagatctca 
      aagatatgt ttcgatata tttttctca tattttgta 
      attacattt atcaataaa actagtctt gtgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY