Status
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Gene information
General information
TC011734 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14232941..14241335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011734
    iBeetle screen iB_09509

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011734 RA
      MATTARFAA GGSSSPPTP TPATSPPAQ TPATQLIVL 
PASLHQSGT GGAIVNGVP VIDVSALEQ AAESSNEST 
ATAEQQGDT HDTDNITQS SPHFITVTV ADGDTVASH 
QSYHIQYVE PEIYASQTN SGQAQMAYP VYTVGESTT 
LYSAGQGQY YAATNTGSS ATAGTVGYS TTSGHYVVQ 
QAVDPDTLI ASTRNSPQT TNAEPSYVA TATVHATQS 
NPVSNEEVG SALGHATRV SPATVQWLL ENYETAEGV 
SLPRSTLYA HYLRHCAEN KLEPVNAAS FGKLIRSVF 
LGLRTRRLG TRGNSKYHY YGIRVKPGS SLMNMDESG 
NRVVLNTSN ASGKAQGTV RPFKRNSDS SDASISVSV 
LSQHVTYLG DGSNAIPAF PDIHFTAES LPDECGYED 
VDTLKSIYR EHLEAFLDA ILSLEFTTI ESLWREFWR 
SQYNNNGDE CEEEKYLSK SKLFCLCSM EAVQQFMKQ 
VDLVFYQNL IQVLVPDVL KPIPATLTQ SIRNFAKNL 
ENWLTSAMA GAPPGMLTV KLSAVSAFS SSLRRYTSL 
NHLAQAARA VLHNSTQIA QMLTDLNRV DFHSVREQA 
SWVCQCDNE IVARFENDF KLTLQQQNS LEQWASWLK 
NVVKSALKP YEGKPSFSK AARQFLLKW SFYSSMVIR 
DLTLRSAAS FGSFHLIRL LYDEYMFYL IEHQVAEAT 
GVVPIAVIL EGIKQDIMQ NSLSIYGND GVCNGGVSA 
KLSCVSPSD HLSKRAKIS
    CDS sequence
    • >TC011734 RA
      atggcaact acagctcga tttgcagca ggaggcagc 
agcagccct cccactcca acgccagcc acttcaccc 
ccggcacag actccggct acccagcta atcgtactg 
cctgctagt ttgcaccag tcgggcacc ggaggggct 
atcgtcaac ggagtgccg gtcatcgac gtctcagca 
ctggagcag gcggcagaa tcaagcaac gagagcaca 
gccactgct gagcaacag ggggataca cacgatacg 
gataatatt acacaaagc tcgccgcat tttatcaca 
gtcacagtt gccgacggt gacacggtg gcatcgcat 
caaagctat cacatacag tatgtggaa cccgagatc 
tacgcgtcc caaacgaac agcggacag gcacaaatg 
gcgtatccg gtgtacact gtgggcgaa tcaacaact 
ttgtattcc gccggtcag gggcagtat tacgccgcc 
accaacacc ggctcctct gccacggca ggcaccgtg 
ggttacagc accacaagt ggccactac gtggtgcaa 
caggccgtc gatcccgac actttgatc gcatcgaca 
cgaaactcc ccacaaaca acaaatgct gaaccgagc 
tatgtagct acagccacc gtgcatgca actcaaagc 
aatccagtg agcaatgag gaggtcggg tcggcgttg 
ggacacgcc acgagggtg tctccggct actgtgcaa 
tggttgttg gagaattac gaaacggcc gaaggtgtg 
tccctgccg cggtctacg ctctatgct cattatttg 
cgccactgt gccgagaat aaattagag ccggtaaac 
gcggcctcg ttcggtaaa ttaatcaga tcggtgttt 
ctcgggttg agaacgcga agactcgga actagaggc 
aactccaag tatcattac tacggaatt cgggtaaaa 
cccggttct tcattgatg aatatggac gaatcgggg 
aacagagtc gtactgaat acatcgaac gcgtcggga 
aaagcccaa ggaacggtt agaccgttt aagagaaat 
tctgattcg tcggatgcg tcgatttcg gtcagtgtt 
ttgtcgcaa catgtgact tatctaggg gatggaagt 
aacgcgatc ccggctttt cccgacata cacttcacg 
gctgagtcg ctacccgac gagtgtggg tatgaagac 
gtcgatact ttgaaatcc atatatagg gaacattta 
gaagccttc ttagatgct atattaagt ttagaattt 
accacaata gagtcgctc tggagggaa ttctggaga 
tcacaatat aacaataac ggcgacgaa tgcgaagaa 
gaaaagtat ctctcgaaa tcgaagttg ttctgtttg 
tgttcgatg gaggctgtc caacagttt atgaaacag 
gtcgatctg gtgttttat cagaatttg atccaagtt 
ttggtacct gacgtactt aagcccata ccggccacc 
ctgacccag tcgattagg aatttcgcc aaaaatttg 
gaaaactgg ctgacgagc gcgatggca ggggcgcct 
cccggcatg ttaacggtg aaactctcg gccgtttcg 
gccttcagt tcgagcctt cgccgctac acatccctc 
aaccactta gcccaagcc gctcgtgcc gtactccac 
aacagcact caaatcgcc caaatgttg accgatttg 
aaccgcgtt gactttcac agcgtgcgt gagcaagcc 
tcctgggtc tgtcagtgc gacaacgag atcgtggct 
cgcttcgaa aacgatttc aagctcact ttgcagcag 
caaaactcg ctcgaacag tgggcctca tggctaaag 
aacgtcgtt aagagcgcg ctcaagccc tacgagggc 
aagccgagc ttctcgaag gcggcgagg cagttcctc 
ctcaagtgg agcttctac agctcgatg gtcattcgg 
gacttgact ctgcggtcg gctgcgagt ttcgggtcg 
ttccacctg atccgcctg ctgtacgac gagtacatg 
ttttatctg attgagcac caggtcgcc gaagctacg 
ggtgtggtt cctattgct gttatcctg gagggaatc 
aaacaggac ataatgcaa aacagtttg tcgatttat 
ggcaacgac ggggtttgc aatggaggt gtgagcgca 
aagttgagt tgtgtgagt ccctctgac catctttcg 
aaaagggcg aaaattagc tag
    mRNA sequence
    • >TC011734 RA
      aatagttgt gaaatcgct tattaatta aaatcgcgc 
ttatcattg ttgtgataa ggtgtagtt tggcggcga 
tcgtgaaag atagcgaaa agcgcgtcg taaggtaag 
ttttcaggt cggtatttg gcgattcca tcgcgatga 
cgtcacagt gtgttgtac ggggtgttg ttgaaaatg 
ttgtataaa tcggtgcat atcgtgaaa tagcgtcgt 
tttgtttgt aaagttttg ttctttttc gtgttggtt 
attgattga attgcgctt gaagccgcc gcgctagct 
cgtcttgta aaatcgcag tttcctgca ttaccggcc 
atggaggcc tcgttgcgt taaataatc aataatata 
aagtcggtc tgttttaat aataatcgg gccttgagg 
cgatgatcg gagtgcgtt taggggtcc cccttgggt 
ggtgagccc ccttggccc gtttcggca acccgccag 
ctgtcaaat cggaggaat ctcattttc gtatccttg 
gagactgcc ttagcaacc gatacaaca acataacaa 
cattagaga gagcatcag gagtagcct tggaaacgc 
agccatggc aactacagc tcgatttgc agcaggagg 
cagcagcag ccctcccac tccaacgcc agccacttc 
acccccggc acagactcc ggctaccca gctaatcgt 
actgcctgc tagtttgca ccagtcggg caccggagg 
ggctatcgt caacggagt gccggtcat cgacgtctc 
agcactgga gcaggcggc agaatcaag caacgagag 
cacagccac tgctgagca acaggggga tacacacga 
tacggataa tattacaca aagctcgcc gcattttat 
cacagtcac agttgccga cggtgacac ggtggcatc 
gcatcaaag ctatcacat acagtatgt ggaacccga 
gatctacgc gtcccaaac gaacagcgg acaggcaca 
aatggcgta tccggtgta cactgtggg cgaatcaac 
aactttgta ttccgccgg tcaggggca gtattacgc 
cgccaccaa caccggctc ctctgccac ggcaggcac 
cgtgggtta cagcaccac aagtggcca ctacgtggt 
gcaacaggc cgtcgatcc cgacacttt gatcgcatc 
gacacgaaa ctccccaca aacaacaaa tgctgaacc 
gagctatgt agctacagc caccgtgca tgcaactca 
aagcaatcc agtgagcaa tgaggaggt cgggtcggc 
gttgggaca cgccacgag ggtgtctcc ggctactgt 
gcaatggtt gttggagaa ttacgaaac ggccgaagg 
tgtgtccct gccgcggtc tacgctcta tgctcatta 
tttgcgcca ctgtgccga gaataaatt agagccggt 
aaacgcggc ctcgttcgg taaattaat cagatcggt 
gtttctcgg gttgagaac gcgaagact cggaactag 
aggcaactc caagtatca ttactacgg aattcgggt 
aaaacccgg ttcttcatt gatgaatat ggacgaatc 
ggggaacag agtcgtact gaatacatc gaacgcgtc 
gggaaaagc ccaaggaac ggttagacc gtttaagag 
aaattctga ttcgtcgga tgcgtcgat ttcggtcag 
tgttttgtc gcaacatgt gacttatct aggggatgg 
aagtaacgc gatcccggc ttttcccga catacactt 
cacggctga gtcgctacc cgacgagtg tgggtatga 
agacgtcga tactttgaa atccatata tagggaaca 
tttagaagc cttcttaga tgctatatt aagtttaga 
atttaccac aatagagtc gctctggag ggaattctg 
gagatcaca atataacaa taacggcga cgaatgcga 
agaagaaaa gtatctctc gaaatcgaa gttgttctg 
tttgtgttc gatggaggc tgtccaaca gtttatgaa 
acaggtcga tctggtgtt ttatcagaa tttgatcca 
agttttggt acctgacgt acttaagcc cataccggc 
caccctgac ccagtcgat taggaattt cgccaaaaa 
tttggaaaa ctggctgac gagcgcgat ggcaggggc 
gcctcccgg catgttaac ggtgaaact ctcggccgt 
ttcggcctt cagttcgag ccttcgccg ctacacatc 
cctcaacca cttagccca agccgctcg tgccgtact 
ccacaacag cactcaaat cgcccaaat gttgaccga 
tttgaaccg cgttgactt tcacagcgt gcgtgagca 
agcctcctg ggtctgtca gtgcgacaa cgagatcgt 
ggctcgctt cgaaaacga tttcaagct cactttgca 
gcagcaaaa ctcgctcga acagtgggc ctcatggct 
aaagaacgt cgttaagag cgcgctcaa gccctacga 
gggcaagcc gagcttctc gaaggcggc gaggcagtt 
cctcctcaa gtggagctt ctacagctc gatggtcat 
tcgggactt gactctgcg gtcggctgc gagtttcgg 
gtcgttcca cctgatccg cctgctgta cgacgagta 
catgtttta tctgattga gcaccaggt cgccgaagc 
tacgggtgt ggttcctat tgctgttat cctggaggg 
aatcaaaca ggacataat gcaaaacag tttgtcgat 
ttatggcaa cgacggggt ttgcaatgg aggtgtgag 
cgcaaagtt gagttgtgt gagtccctc tgaccatct 
ttcgaaaag ggcgaaaat tagctagct ttcaaaagg 
ggcgaagtt tatcgtgac atttcaata agttggttc 
ggttggtta tgttgcggt aaatacggg accattatc 
ttttcgttt cttcatagt ttctcatta atttatttc 
acgtattta ggatgtgta atttaaatg tttgaattg 
tgcaattgg ttatgaagc atgtataag tgccgtcaa 
atttgtgtg acaataggt gtatatatt atacttata 
tatgtacat tgccgctaa aggaattct gtaattata 
agtcactga gatcaagag ttcttagac tgatttcgt 
tggattttg ttgtgtgta cataattat ttattgttt 
tttttttcg tttctgtgt gttaataat tgttatttt 
ttctgcaag tgttttatt tcggttttc ggtgatttg 
attcgacga gtttattgc tcaaaagtg taatccatc 
cattaaaat ataatagtt gaacagacg actacgcag 
ggtatgaat attcctcta agcagtaca agtaagtgt 
ttgatgtta ttcttgcta aagccccca ttcataaat 
tgtctcact ggtagtttc accaatcat gcttgaaac 
aatcgcaat tggctacta cagggtaaa aaatggtct 
ccattgttt tactactgc tggcgacaa atatgacgg 
gtcgacttt caaccggtt gcctgatgt acatttatt 
tgtaaaaaa aagctaatt ttgtatttt ccaaataaa 
tgtggcttt tatttgacg aatattcgt gtgcttcat 
cgactatgt gtgattgct ggatgcact ctttcagtt 
cggtagcag tagggaatt ccatgccaa aacgacact 
ttgaacccc atctgcgat ttcgccaga tttgagact 
tcgccggct ttgtttcgt acaacagtg ccaagaaaa 
tcgccgttt aaacgaaaa gtaatcatg ggcatctca 
ctcgctatt gctcgtacg tgctttaaa cagtatcaa 
caaattatt tttcattcc ttaacttgt tttaaaaaa 
aagcggtga attagggcc attggtctc ctcgaattt 
caagatcgc agatggggc tcaagaagt ttcgcacgg 
aactgctca gtagccttc gattttgac tgctactgg 
tcattgtat ctagagaga aagatctgt gtatttttg 
tagatgtca gtataagaa actgcttta acccacttt 
tctgttggc cgctttgcg aaagtcggt tagatctca 
aagatatgt ttcgatata tttttctca tattttgta 
attacattt atcaataaa actagtctt gtgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY