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Gene information
General information
TC011770 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13728522..13732559
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011770
    iBeetle screen iB_09506

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011770 RA
      MGQIIFVWF ILLKLATSL ELKSYVSDH GLHGTITFR 
      KIGPEIKII TDLNATLEY PNQVWSWAV VEFPVDYSE 
      LENRCWESK LGRELVNLD TIYGPLVLP DNGTSEFST 
      QELQINGDV GIYGKSLLF KDPETNTRI CATITMVDK 
      AQEKIAVAR FNSPIAGSI HFRWFSTKD NHNDMVVTT 
      DLYHVSNKE NHSQSLDFT EHLWKIYAT DILDSDKER 
      PDCNFLQLV FDPDNTGDQ ALGDLDSRL GKIKISTDY 
      KRKKFKTLF TDQRLILLP SDLQGPQRS LYLVIFERK 
      HPDSFLACA KIRYERPIN AKVIIQSGG IKGEVRMTQ 
      FSKFEPTFV NFDLTTARG DLETRLVYS SSVAGYKIH 
      ELPVKPSIT LGQNENSCL TTKFVYNPV KIDPNVAPP 
      SGLGTQNQY LVGDLSGKL QGRNNQTAL VPTGHELSG 
      LYWDTLLPL QGKFSIIHR TLVIYKNTQ YPKGEILSE 
      PWICGGIAL YEPNFRYQK PIFTAEVIF RYPTVGRVL 
      MRQVRDEPW SDTSILVEY LVHADGAAL NNSKDHRWA 
      IHEEPPGKD FYSWKERCL SSGQIYNPY KIDALDACT 
      GGLCRVGDL TARLGTVEI SGKIADSDR VSRTMWTDP 
      FVPLTGQFS VLGKSLVVY DDFGPKARG ERLACSIIG 
      GVYRCKAVV KDWFPNGVP ISVKGKIEF YQQTEYDIT 
      NVEVNLQGL SDNSGYHIH ITPVEENLE FPCEASSLY 
      DHWNPLNVD PASSPRTYH GTPDQYEMG DLSGKFGTL 
      DNQTYYKTD YNDTMLPLF GPKSIVGRS IIIHKKDTS 
      RWACATIER GYSPSEARE LRAIASFHH PDGYAYGYL 
      KMKQLIYND GSKSDTTIE VKLRHPGKN DRNVTRDHD 
      WAIYVNPVG VDAAVKTQN TRCVAGGYI WNPYFTQLA 
      DPLNGDLYR QECGPENPL RCHVGDLSA RLGTIDIGL 
      QRKVFTDAN FPLEGDVTA IGRSIVIMS PNRRPERYA 
      CANIEPDYD IIKYANIEK PPRFVLSQF IEEVREILG 
      IPDWFLTVD SRKTKSLHS DACVQLLLH FKGPLANRL 
      EQDFSRLLA SGRLDTPTL YIPGFINTK RKVKIAYKQ 
      CGVQDPNEK SKKRTGFFF ARSGSNPAK FAHSLIVVI 
      VLSYLL
    CDS sequence
    • >TC011770 RA
      atgggacaa attatcttc gtttggttt attttgctc 
      aaacttgca acgtctctg gaattgaaa agttatgtt 
      tcggaccat gggctgcac ggcaccatc acgttcagg 
      aaaatcggc cccgaaatc aaaattatc accgatttg 
      aacgcgact ttggagtac cctaaccag gtgtggtcg 
      tgggctgtg gtggagttc ccagtggat tactccgaa 
      ctggagaat cggtgttgg gagagcaaa ctgggcagg 
      gaattggtc aatttggac accatctac ggccctttg 
      gtactcccc gataacggc accagtgag tttagtacc 
      caggagttg caaatcaac ggagatgtg ggcatttat 
      ggcaaatct ctcctcttc aaagaccca gagaccaac 
      accaggatc tgcgcaacc atcaccatg gtggacaaa 
      gcgcaggag aagatcgca gtagcgcgg ttcaactcg 
      cccatcgct gggagcatc cacttcagg tggttctcc 
      accaaggac aaccacaac gacatggtg gtaaccacc 
      gacttgtac cacgtctcc aacaaggag aaccacagc 
      caaagtctg gacttcacc gagcatttg tggaagatt 
      tacgccact gatatccta gacagtgac aaggagcga 
      cccgactgc aacttcctg cagctggtc ttcgacccg 
      gacaacacc ggggaccaa gccctgggc gatttggac 
      tcaagactg ggcaaaatc aagatctcc accgactac 
      aagcgcaaa aaattcaag actttgttc accgaccag 
      cgcctcatc ctcctcccg agcgacctc caggggccc 
      caaaggagc ctctacctg gtgatcttc gagcgcaaa 
      caccccgac agcttcctg gcctgcgcc aaaattaga 
      tacgagcgc ccaatcaat gccaaggtt ataatacag 
      tccgggggg atcaaaggg gaggtcagg atgacgcag 
      tttagcaaa ttcgagccc actttcgtc aattttgac 
      ttgaccacg gccaggggc gacttggag acgcgtctg 
      gtgtacagc tcgtccgtc gctggatac aaaatacac 
      gaattgcca gttaaaccg tccataacg ctgggtcag 
      aacgagaac tcgtgtctc acaacgaaa tttgtctac 
      aacccggtt aaaatcgac cccaatgtg gcaccgcca 
      agtgggctg gggacccag aaccagtac ctggtgggc 
      gacttgtcg gggaaactg caagggagg aacaaccaa 
      acggcgttg gtgcccacg gggcacgaa ctcagcggc 
      ctctactgg gacacgctc ctcccgctc caggggaag 
      ttctccata atacaccgc acgctagtt atttacaaa 
      aacacgcag taccccaaa ggcgagatc ctctccgag 
      ccttggatc tgcggcgga atcgcgctc tacgagccc 
      aacttccgg taccaaaag cccattttc acggccgag 
      gtgatcttc cggtacccc accgtgggg cgcgttttg 
      atgcgacag gtgcgggac gaaccttgg agcgacacc 
      agcatcctg gtggagtac ctggtgcat gccgacggt 
      gccgctttg aacaactca aaggaccat cgatgggcc 
      atccatgag gagcccccc ggcaaggac ttctacagc 
      tggaaggag cgctgtctc agctcgggt caaatctac 
      aacccctat aaaatcgac gctttggac gcgtgtact 
      gggggtctg tgccgagtt ggggatctg acagccaga 
      cttgggact gttgaaatt tcgggcaag attgcagac 
      agtgacagg gtctcgcgc acgatgtgg actgaccct 
      tttgtgccc ttgacgggc cagttcagc gttttgggc 
      aaatcgctg gtagtttac gacgatttc gggccgaag 
      gcacgaggc gaacgcttg gcgtgctcc attattggg 
      ggagtttat agatgcaaa gctgtggtc aaggattgg 
      ttcccgaac ggagtaccg atctcagtg aagggcaaa 
      atcgagttt taccaacag accgagtac gacattacc 
      aacgttgaa gtaaatttg caaggtttg agcgacaac 
      agtgggtac cacatccat attactcca gttgaggag 
      aatttggag tttccgtgc gaggcatca agtctttac 
      gaccattgg aaccctctg aatgtggac ccagcgtcg 
      tccccgcgc acctaccat ggtaccccc gaccagtac 
      gagatgggc gacctgagc ggcaaattc ggcactctg 
      gacaaccaa acctactac aaaaccgac tacaacgac 
      accatgctc ccattattc ggccccaaa agcatagtt 
      ggacgttcc ataatcatc cataaaaag gacacgtcc 
      agatgggct tgcgcgacc attgagcgt ggctatagc 
      ccatcggaa gcccgggaa ctgcgcgca attgcctcg 
      ttccaccac ccagatggg tacgcttat ggatattta 
      aaaatgaaa caactaatt tacaatgac ggtagcaag 
      agtgacacc acgatcgaa gtcaaactg cggcatcct 
      gggaaaaac gaccggaat gtgaccagg gaccatgac 
      tgggccatt tacgtgaac ccagtgggc gtggacgcg 
      gccgtcaag acccagaac acgcggtgt gtggccggg 
      ggctacatc tggaacccc tatttcacc caactggcc 
      gaccccctc aatggtgat ctttatcgg caagagtgc 
      gggcctgag aacccgctc aggtgccac gtgggggac 
      ctgtcggcc cgtttgggg accatcgac atcgggctc 
      cagcgcaaa gtcttcacc gatgcgaat ttcccgctc 
      gaaggcgac gtcacggca atcgggcgc tccatcgtc 
      atcatgagc cccaacaga cgtcccgaa cgttacgcc 
      tgtgccaat atcgagccc gactatgac ataatcaag 
      tacgcaaac atcgaaaag cccccacgt tttgtcctg 
      tcgcagttt atcgaagaa gtgcgcgaa attctggga 
      atccccgac tggtttttg accgtagat tcgcgcaaa 
      accaagtct ttgcacagc gacgcttgc gtccagctt 
      ttgctgcat tttaaagga ccgctagct aaccgatta 
      gagcaggat ttcagccga ttgctggca agtgggcgt 
      ttagacacc cccacattg tacattcca gggtttatt 
      aatacgaaa cgtaaggtg aaaattgcg tacaagcag 
      tgtggagta caagacccc aacgagaag agtaaaaaa 
      cggaccgga tttttcttt gcacgctca ggaagcaac 
      ccagccaaa tttgctcac agtctgatt gtagtgata 
      gttttaagc tatttattg taa
    mRNA sequence
    • >TC011770 RA
      agacaggta cccattcat tcaccacag gtaagaacc 
      gttggtaag tatgtgatt agtcagttc ccgctttct 
      attcatcga gaatatcga atttacgac caacgcgtt 
      cagttttct accatctgg aggcgaaac gagacgcaa 
      aatgtgacg cgaaatggg acaaattat cttcgtttg 
      gtttatttt gctcaaact tgcaacgtc tctggaatt 
      gaaaagtta tgtttcgga ccatgggct gcacggcac 
      catcacgtt caggaaaat cggccccga aatcaaaat 
      tatcaccga tttgaacgc gactttgga gtaccctaa 
      ccaggtgtg gtcgtgggc tgtggtgga gttcccagt 
      ggattactc cgaactgga gaatcggtg ttgggagag 
      caaactggg cagggaatt ggtcaattt ggacaccat 
      ctacggccc tttggtact ccccgataa cggcaccag 
      tgagtttag tacccagga gttgcaaat caacggaga 
      tgtgggcat ttatggcaa atctctcct cttcaaaga 
      cccagagac caacaccag gatctgcgc aaccatcac 
      catggtgga caaagcgca ggagaagat cgcagtagc 
      gcggttcaa ctcgcccat cgctgggag catccactt 
      caggtggtt ctccaccaa ggacaacca caacgacat 
      ggtggtaac caccgactt gtaccacgt ctccaacaa 
      ggagaacca cagccaaag tctggactt caccgagca 
      tttgtggaa gatttacgc cactgatat cctagacag 
      tgacaagga gcgacccga ctgcaactt cctgcagct 
      ggtcttcga cccggacaa caccgggga ccaagccct 
      gggcgattt ggactcaag actgggcaa aatcaagat 
      ctccaccga ctacaagcg caaaaaatt caagacttt 
      gttcaccga ccagcgcct catcctcct cccgagcga 
      cctccaggg gccccaaag gagcctcta cctggtgat 
      cttcgagcg caaacaccc cgacagctt cctggcctg 
      cgccaaaat tagatacga gcgcccaat caatgccaa 
      ggttataat acagtccgg ggggatcaa aggggaggt 
      caggatgac gcagtttag caaattcga gcccacttt 
      cgtcaattt tgacttgac cacggccag gggcgactt 
      ggagacgcg tctggtgta cagctcgtc cgtcgctgg 
      atacaaaat acacgaatt gccagttaa accgtccat 
      aacgctggg tcagaacga gaactcgtg tctcacaac 
      gaaatttgt ctacaaccc ggttaaaat cgaccccaa 
      tgtggcacc gccaagtgg gctggggac ccagaacca 
      gtacctggt gggcgactt gtcggggaa actgcaagg 
      gaggaacaa ccaaacggc gttggtgcc cacggggca 
      cgaactcag cggcctcta ctgggacac gctcctccc 
      gctccaggg gaagttctc cataataca ccgcacgct 
      agttattta caaaaacac gcagtaccc caaaggcga 
      gatcctctc cgagccttg gatctgcgg cggaatcgc 
      gctctacga gcccaactt ccggtacca aaagcccat 
      tttcacggc cgaggtgat cttccggta ccccaccgt 
      ggggcgcgt tttgatgcg acaggtgcg ggacgaacc 
      ttggagcga caccagcat cctggtgga gtacctggt 
      gcatgccga cggtgccgc tttgaacaa ctcaaagga 
      ccatcgatg ggccatcca tgaggagcc ccccggcaa 
      ggacttcta cagctggaa ggagcgctg tctcagctc 
      gggtcaaat ctacaaccc ctataaaat cgacgcttt 
      ggacgcgtg tactggggg tctgtgccg agttgggga 
      tctgacagc cagacttgg gactgttga aatttcggg 
      caagattgc agacagtga cagggtctc gcgcacgat 
      gtggactga cccttttgt gcccttgac gggccagtt 
      cagcgtttt gggcaaatc gctggtagt ttacgacga 
      tttcgggcc gaaggcacg aggcgaacg cttggcgtg 
      ctccattat tgggggagt ttatagatg caaagctgt 
      ggtcaagga ttggttccc gaacggagt accgatctc 
      agtgaaggg caaaatcga gttttacca acagaccga 
      gtacgacat taccaacgt tgaagtaaa tttgcaagg 
      tttgagcga caacagtgg gtaccacat ccatattac 
      tccagttga ggagaattt ggagtttcc gtgcgaggc 
      atcaagtct ttacgacca ttggaaccc tctgaatgt 
      ggacccagc gtcgtcccc gcgcaccta ccatggtac 
      ccccgacca gtacgagat gggcgacct gagcggcaa 
      attcggcac tctggacaa ccaaaccta ctacaaaac 
      cgactacaa cgacaccat gctcccatt attcggccc 
      caaaagcat agttggacg ttccataat catccataa 
      aaaggacac gtccagatg ggcttgcgc gaccattga 
      gcgtggcta tagcccatc ggaagcccg ggaactgcg 
      cgcaattgc ctcgttcca ccacccaga tgggtacgc 
      ttatggata tttaaaaat gaaacaact aatttacaa 
      tgacggtag caagagtga caccacgat cgaagtcaa 
      actgcggca tcctgggaa aaacgaccg gaatgtgac 
      cagggacca tgactgggc catttacgt gaacccagt 
      gggcgtgga cgcggccgt caagaccca gaacacgcg 
      gtgtgtggc cgggggcta catctggaa cccctattt 
      cacccaact ggccgaccc cctcaatgg tgatcttta 
      tcggcaaga gtgcgggcc tgagaaccc gctcaggtg 
      ccacgtggg ggacctgtc ggcccgttt ggggaccat 
      cgacatcgg gctccagcg caaagtctt caccgatgc 
      gaatttccc gctcgaagg cgacgtcac ggcaatcgg 
      gcgctccat cgtcatcat gagccccaa cagacgtcc 
      cgaacgtta cgcctgtgc caatatcga gcccgacta 
      tgacataat caagtacgc aaacatcga aaagccccc 
      acgttttgt cctgtcgca gtttatcga agaagtgcg 
      cgaaattct gggaatccc cgactggtt tttgaccgt 
      agattcgcg caaaaccaa gtctttgca cagcgacgc 
      ttgcgtcca gcttttgct gcattttaa aggaccgct 
      agctaaccg attagagca ggatttcag ccgattgct 
      ggcaagtgg gcgtttaga cacccccac attgtacat 
      tccagggtt tattaatac gaaacgtaa ggtgaaaat 
      tgcgtacaa gcagtgtgg agtacaaga ccccaacga 
      gaagagtaa aaaacggac cggattttt ctttgcacg 
      ctcaggaag caacccagc caaatttgc tcacagtct 
      gattgtagt gatagtttt aagctattt attgtaatg 
      atatgactg ttcctaata aaaaagtaa tatatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY