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Gene information
General information
TC011784 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13526203..13528624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011784
    iBeetle screen iB_09503

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011784 RA
      MEEVPLVRS CTQVPLENF WKAVLVYHN RPHVINRKL 
      AAIVPLIFC KITLNCPGV KRLRDVFTW ESLIYEVRK 
      LPDLSQITP QFIKNITEI YDKKATVEE TALETLDNC 
      YEGTFLSIR QLLSRKNNE KCLEIAIFD KDTQTVTFL 
      AAQEQTSPI IAPRFPYHI HLTKSGHLT IILNEFEIA 
      ETASAEWLA DKLFPKLMK WSENDISDD SVIKSLSLV 
      APSDYCTLY SELKVKYGR DLVEKWPQK AKTDPQKYV 
      FEDIAIAAY LISLWRHLK TENINFVDC GCGNGLLVY 
      ILNKEGFKG CGFDIRSRQ VWEIYGGEA DLRTGAVTP 
      ESIYPDATW LIGNHSDEL TPWIPIIAL NSSPKTNFF 
      VLPCCPYDL SGRKYVRIN AALSQYGDY LDYVKNIST 
      MCGFTTSVD KLRIPSTKR TCLVGNKNE PELEETRQK 
      MKEFVEKNT RDFVPRAAV EKVRNCTRL DPDLKKKII 
      NLIVDELLQ TPCDSEISD WKTGGTMSL EELSQKISS 
      EDRKALKNE CGGLQTLIK NHRYIFELR KTWVTLRAP 
      QTLKQTDKY KDKPCWFHE NYPYGCLHS AEICCYKHV 
      EKN
    CDS sequence
    • >TC011784 RA
      atggaggag gtccccctg gtcaggagt tgcactcaa 
      gtccctttg gaaaatttc tggaaggct gttttagtt 
      taccacaat aggccccac gtgatcaat cgcaaatta 
      gcagcgatt gtgcccctt atcttttgc aaaatcaca 
      ctcaactgc cctggcgtt aaacgcctg agggacgtt 
      ttcacctgg gagtccctc atatacgaa gttagaaag 
      ttacccgac ttgtcccaa atcactcca caatttatt 
      aaaaatatc acggaaatt tacgacaaa aaagccaca 
      gttgaggaa acggcgcta gagacattg gataattgt 
      tacgagggc acttttcta tcaattcga cagttactt 
      tcgcgcaaa aacaacgaa aaatgcctt gaaatcgca 
      atttttgat aaagacacc caaactgtt acttttctc 
      gccgctcaa gaacaaacc agtccgatc atagccccc 
      cgatttccc taccacatc cacttgacc aaatcggga 
      cacctgact atcattttg aacgaattt gaaattgcc 
      gaaacggca agtgctgaa tggcttgcc gataaatta 
      tttccaaaa ttgatgaaa tggagcgaa aacgacatt 
      agtgacgat tctgtgata aaatctctg agtcttgta 
      gcccctagt gactactgc acgctttac tccgaattg 
      aaggtgaaa tacggcaga gatttggtc gaaaagtgg 
      ccacaaaaa gccaaaact gatccccaa aaatacgtt 
      tttgaggac atcgcaatc gcggcttac ttgatcagt 
      ttatggcgg catttaaag actgaaaat atcaatttc 
      gtggactgt gggtgcggc aatggtctc ctcgtctat 
      attttaaac aaggagggt ttcaagggc tgtggtttc 
      gatattcga agtcggcaa gtttgggag atttacggg 
      ggcgaagcc gaccttcgc acaggtgca gtcacccca 
      gagtcaatt tatccggac gccacttgg ctcattggt 
      aatcactcg gacgagcta acaccttgg attccgatc 
      attgcactc aacagttca cccaagacg aattttttc 
      gttctgcct tgttgcccg tacgactta agtggtcgc 
      aaatatgtt agaattaac gctgcatta agccagtat 
      ggggactat ttggattat gttaaaaat atttccaca 
      atgtgtgga tttacgacg tcggttgac aagctgagg 
      attccgtca acgaaaaga acttgcctt gtgggaaac 
      aaaaatgag cccgagctt gaggaaact aggcaaaaa 
      atgaaggaa tttgttgaa aaaaataca agggacttt 
      gtgccgcga gcagcggtc gagaaagtt cgtaactgt 
      acgaggctg gacccggat ttgaagaaa aaaataatt 
      aatttgatc gttgacgaa ttacttcaa acaccttgt 
      gattccgaa atctcggac tggaaaacc ggggggaca 
      atgtctttg gaagaattg agccagaaa atttcaagc 
      gaggaccgc aaggcgctt aaaaatgaa tgtgggggg 
      ctccaaact ctaataaaa aatcacaga tatattttc 
      gaactgcgc aaaacttgg gttacacta cgtgccccc 
      caaaccctt aaacaaacc gacaaatac aaggacaaa 
      ccctgctgg ttccacgaa aattacccg tacggttgc 
      ttgcactcc gctgaaatt tgttgttac aaacatgta 
      gaaaagaat taa
    mRNA sequence
    • >TC011784 RA
      acttatcac aatattggc gcaaaagtg tgctcactt 
      ggggccgga ataaacggc ggaagacca tcgtttgct 
      gggaaattg acggatggt gaggttagg gtggcggtg 
      tcaaaagtt acgtcactc acacccgga aaactacga 
      taattagcg caaatcaag caaatgcgt tcacaagcc 
      aagctctac cagcatttt aattaattg cgaacggtc 
      cgatttgat tatttcatc agtggtgtc acgggttca 
      attttcggc gaacataac cccaaacta tgtccaaga 
      cttgcatgc aattctttc gctttagtc actgcacca 
      gtaaagccg atacgtccc cgaaggccc tataccggc 
      acttgatac gccagtgtg agaaactgc ccgaagggc 
      cccaaaaat attacaagt ttatgatca aacaggtaa 
      gtaagttcg tgccaagtt ggcggagtc acccaaata 
      ttcgattta agagtttca aacagcacg ttaatgaaa 
      ccgaccccc aacgcatcc aacaaatcg tcgcaaggt 
      cttacgagg acgctgact gggtcttga aaaaatatc 
      ttgggaaac agtaacatg gaggaggtc cccctggtc 
      aggagttgc actcaagtc cctttggaa aatttctgg 
      aaggctgtt ttagtttac cacaatagg ccccacgtg 
      atcaatcgc aaattagca gcgattgtg ccccttatc 
      ttttgcaaa atcacactc aactgccct ggcgttaaa 
      cgcctgagg gacgttttc acctgggag tccctcata 
      tacgaagtt agaaagtta cccgacttg tcccaaatc 
      actccacaa tttattaaa aatatcacg gaaatttac 
      gacaaaaaa gccacagtt gaggaaacg gcgctagag 
      acattggat aattgttac gagggcact tttctatca 
      attcgacag ttactttcg cgcaaaaac aacgaaaaa 
      tgccttgaa atcgcaatt tttgataaa gacacccaa 
      actgttact tttctcgcc gctcaagaa caaaccagt 
      ccgatcata gccccccga tttccctac cacatccac 
      ttgaccaaa tcgggacac ctgactatc attttgaac 
      gaatttgaa attgccgaa acggcaagt gctgaatgg 
      cttgccgat aaattattt ccaaaattg atgaaatgg 
      agcgaaaac gacattagt gacgattct gtgataaaa 
      tctctgagt cttgtagcc cctagtgac tactgcacg 
      ctttactcc gaattgaag gtgaaatac ggcagagat 
      ttggtcgaa aagtggcca caaaaagcc aaaactgat 
      ccccaaaaa tacgttttt gaggacatc gcaatcgcg 
      gcttacttg atcagttta tggcggcat ttaaagact 
      gaaaatatc aatttcgtg gactgtggg tgcggcaat 
      ggtctcctc gtctatatt ttaaacaag gagggtttc 
      aagggctgt ggtttcgat attcgaagt cggcaagtt 
      tgggagatt tacgggggc gaagccgac cttcgcaca 
      ggtgcagtc accccagag tcaatttat ccggacgcc 
      acttggctc attggtaat cactcggac gagctaaca 
      ccttggatt ccgatcatt gcactcaac agttcaccc 
      aagacgaat tttttcgtt ctgccttgt tgcccgtac 
      gacttaagt ggtcgcaaa tatgttaga attaacgct 
      gcattaagc cagtatggg gactatttg gattatgtt 
      aaaaatatt tccacaatg tgtggattt acgacgtcg 
      gttgacaag ctgaggatt ccgtcaacg aaaagaact 
      tgccttgtg ggaaacaaa aatgagccc gagcttgag 
      gaaactagg caaaaaatg aaggaattt gttgaaaaa 
      aatacaagg gactttgtg ccgcgagca gcggtcgag 
      aaagttcgt aactgtacg aggctggac ccggatttg 
      aagaaaaaa ataattaat ttgatcgtt gacgaatta 
      cttcaaaca ccttgtgat tccgaaatc tcggactgg 
      aaaaccggg gggacaatg tctttggaa gaattgagc 
      cagaaaatt tcaagcgag gaccgcaag gcgcttaaa 
      aatgaatgt ggggggctc caaactcta ataaaaaat 
      cacagatat attttcgaa ctgcgcaaa acttgggtt 
      acactacgt gccccccaa acccttaaa caaaccgac 
      aaatacaag gacaaaccc tgctggttc cacgaaaat 
      tacccgtac ggttgcttg cactccgct gaaatttgt 
      tgttacaaa catgtagaa aagaattaa ttttttatt 
      tgtaaaaaa gtttgaaca ataaaattt ttgaagcat 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY