Status
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Gene information
General information
TC011817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13143684..13148228
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011817
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_09502

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011817 RA
        MAGIFDIEL HDGLPQSSD DEDDAIDIA EDQYDEQPD 
        VSDMIDTAD TERVQVDEQ SVNKGKKIG NEDFRILSV 
        LGKGGYGKV FQVIKETGS DKESIYAMK ILHKASIVR 
        NQKDTAHTK AERNILEAV KHPFIVELI YAYQTNGKL 
        YLILEFLQG GELFTMLEK EGLLLEETA IFYLSEILL 
        AIEHLHFLG IVYRDLKPE NVLLDSKGH VKLTDFGLC 
        KEHIRDGVL THTFCGTIE YMAPEILTR SGHGKEVDW 
        WSFGALAYD MLTGAPPFQ GDTRKKTIE KILRGKLFL 
        PPYLTQDSK DLIRKLLKR QVSARLGTV EGASAIKNH 
        PFFKNVNWD DVLAKRITP PVPESMLKK KEDDTSNFD 
        CKFTKQTPV DSPDDSTLS ESANEVFKG FTYVAPSVL 
        EDMYKSSVK SRPRRQQSR LETRTPSHI VDHNYVNNH 
        GEQFRVDNT YLNNHVAAG PARFEPYNQ LDNHVRGIP 
        MNVNNHVIA GPAPRVINH FEPFGQLDN HDAVMGGPS 
        MDTNSVRLD NYTGXXXXX XXXXXXXXX XXXPAVSSP 
        QCE
      CDS sequence
      • >TC011817 RA
        atggcagga atcttcgat attgagtta cacgacggg 
        ctgccccag agctccgac gacgaggac gatgccata 
        gacatcgca gaggatcaa tacgacgag cagccagat 
        gtttccgac atgattgac acggccgac accgagcgg 
        gtacaggtg gacgagcag agcgtgaac aaggggaag 
        aagatcggg aacgaggat ttccgcata ctcagcgtc 
        ttggggaaa gggggctac ggcaaggtg tttcaagtg 
        atcaaagag acgggaagc gataaggaa agcatttac 
        gcgatgaaa atcctacac aaggcttcc attgtacgc 
        aaccagaaa gacactgca cacactaag gctgaacgc 
        aatatttta gaagctgtc aagcatcct tttatagtc 
        gaattaatt tacgcctac caaacgaac ggcaaactc 
        tatttaata ctagaattt ttacaaggg ggcgagctc 
        ttcaccatg ctggagaaa gaggggctc ctgttagaa 
        gaaacagcc attttttac ctatctgag attttactc 
        gccatagaa catttgcat tttctcggg atcgtttac 
        cgggatttg aagccggaa aacgtcctg ttagattct 
        aaaggtcac gttaaattg accgatttc ggattgtgc 
        aaggaacat atcagggat ggtgttttg acgcatact 
        ttttgcgga acaattgaa tacatggcg cctgagatt 
        ttgacgagg agtgggcac gggaaagag gtggattgg 
        tggtcgttt ggggctctg gcctacgac atgttgacg 
        ggagctcca ccgtttcaa ggtgatact cgtaaaaag 
        acaatagag aagattctc cgaggtaaa ttattcctt 
        ccgccgtac ctaacccaa gactccaaa gatttaatt 
        cgcaaatta ttgaaacgt caagtcagc gcccgtctg 
        ggcactgtc gaaggcgcc tcggcaata aaaaatcat 
        ccatttttc aagaacgtc aattgggac gacgttctc 
        gccaaacga ataacgcca ccagtgccc gaaagtatg 
        ctaaaaaag aaagaggac gacacgtcc aactttgac 
        tgcaaattc accaaacaa acgccggtc gactcgccc 
        gacgactcg accctgagc gagagcgcc aacgaagtc 
        ttcaagggt ttcacctac gtggcgccc tctgtcctc 
        gaggacatg tacaagagc agcgtcaag agccggcct 
        cgacgccag caaagccgt ctcgagacg cgcacgccc 
        tctcacatc gtcgaccac aactacgtc aacaaccac 
        ggcgagcaa tttcgggtg gataacacg tacttgaat 
        aatcacgtg gccgcaggc cccgcccgc ttcgagccg 
        tacaaccaa ttggacaac cacgtccgg gggattccc 
        atgaatgtg aacaatcac gtgattgcg ggccccgcc 
        cctcgggtc atcaaccac tttgagccg ttcggccaa 
        ttggataat catgacgct gtgatgggc gggccctcg 
        atggatacg aattcggtt aggttagat aattacacg 
        ggggnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
        nnnnnnnnn nnnnnnnnc cccgccgtt tcaagtccg 
        caatgcgaa taa
      mRNA sequence
      • >TC011817 RA
        tcgcattgg cacgcctgg aatccgaca taacccaat 
        attcttgag tggtccgtg gtttgactg tggtgttgt 
        cttggtgga tttcggcgc atttcggcg aaaaatccg 
        cactgtggg tgcaaattg gtgcaaagc gcgctcgcc 
        tcacccgtt tggtgttat gttcagtgc gaatcagtg 
        cgtggtttg aggaggaat catggcagg aatcttcga 
        tattgagtt acacgacgg gctgcccca gagctccga 
        cgacgagga cgatgccat agacatcgc agaggatca 
        atacgacga gcagccaga tgtttccga catgattga 
        cacggccga caccgagcg ggtacaggt ggacgagca 
        gagcgtgaa caaggggaa gaagatcgg gaacgagga 
        tttccgcat actcagcgt cttggggaa agggggcta 
        cggcaaggt gtttcaagt gatcaaaga gacgggaag 
        cgataagga aagcattta cgcgatgaa aatcctaca 
        caaggcttc cattgtacg caaccagaa agacactgc 
        acacactaa ggctgaacg caatatttt agaagctgt 
        caagcatcc ttttatagt cgaattaat ttacgccta 
        ccaaacgaa cggcaaact ctatttaat actagaatt 
        tttacaagg gggcgagct cttcaccat gctggagaa 
        agaggggct cctgttaga agaaacagc catttttta 
        cctatctga gattttact cgccataga acatttgca 
        ttttctcgg gatcgttta ccgggattt gaagccgga 
        aaacgtcct gttagattc taaaggtca cgttaaatt 
        gaccgattt cggattgtg caaggaaca tatcaggga 
        tggtgtttt gacgcatac tttttgcgg aacaattga 
        atacatggc gcctgagat tttgacgag gagtgggca 
        cgggaaaga ggtggattg gtggtcgtt tggggctct 
        ggcctacga catgttgac gggagctcc accgtttca 
        aggtgatac tcgtaaaaa gacaataga gaagattct 
        ccgaggtaa attattcct tccgccgta cctaaccca 
        agactccaa agatttaat tcgcaaatt attgaaacg 
        tcaagtcag cgcccgtct gggcactgt cgaaggcgc 
        ctcggcaat aaaaaatca tccattttt caagaacgt 
        caattggga cgacgttct cgccaaacg aataacgcc 
        accagtgcc cgaaagtat gctaaaaaa gaaagagga 
        cgacacgtc caactttga ctgcaaatt caccaaaca 
        aacgccggt cgactcgcc cgacgactc gaccctgag 
        cgagagcgc caacgaagt cttcaaggg tttcaccta 
        cgtggcgcc ctctgtcct cgaggacat gtacaagag 
        cagcgtcaa gagccggcc tcgacgcca gcaaagccg 
        tctcgagac gcgcacgcc ctctcacat cgtcgacca 
        caactacgt caacaacca cggcgagca atttcgggt 
        ggataacac gtacttgaa taatcacgt ggccgcagg 
        ccccgcccg cttcgagcc gtacaacca attggacaa 
        ccacgtccg ggggattcc catgaatgt gaacaatca 
        cgtgattgc gggccccgc ccctcgggt catcaacca 
        ctttgagcc gttcggcca attggataa tcatgacgc 
        tgtgatggg cgggccctc gatggatac gaattcggt 
        taggttaga taattacac gggggnnnn nnnnnnnnn 
        nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
        ccccgccgt ttcaagtcc gcaatgcga ataacgacg 
        acttgatgt ttatcgatc gcaggcttt cgatgatgg 
        atgtcagtc atgatctac ctcctgtat aatagtgtg 
        gcatttttg ggttcaaac gcaatgttt agttcaatt 
        ttgtttata taatttatt tgttgtggt taccaatag 
        tttaatcga gaggaaagt ggacgttga gtgatttta 
        acttaatta gagaattat agttaggtc aattttttc 
        ttttcgtta ccaatatta tttaacctt gccgcggat 
        aacattatg atgattctc gagatttga tttcttcgt 
        ggagagatg tcgtaggtt aaggttcgt tgtttggtc 
        aagtgtgta gtgcactcg gaacgcatt gtagaattg 
        taaaaatta attgttgta aattaacta agttgtctc 
        gtggagttc ttggaaaga tagtttcgg tgtggttaa 
        cggttggct ctccaaacc ggcactaat ataactgta 
        tcgcgtttt atatcaatt tctcctcta agctttgta 
        ttgtttttt atgatattt aaacatatt aggtagtgg 
        tggggcttc aaattactt agttaattt tttttttga 
        acaaaaata ataaaatta ttatatgac aaggtgctt 
        ttattttaa ctttctttc acgttattt acgggttgt 
        tttatttaa gtgtaggta actcacctt gcactctaa 
        actttcaaa tttgtgttc gaaatgata ttacgaaac 
        ccctatgag gtgcaaaaa tgtgttcat tataagata 
        aatttacac caatataga cttttttat acatgaatg 
        taaacgtta taattgaag ctaaaagtg tttaggcac 
        aagctggtc ataatttgt gcgagatgc aaggttggt 
        tattgtata gatgatgtc taaagatgt agtataatt 
        ctagttaag tggaatatg ttattcata ttaatcttt 
        tatatacat atttatctt catacttaa ataatttat 
        attttacga tatttatgt tgggttttg gcccatccg 
        aaaaatggg tgatattgt ttcaagctg ggttgattc 
        ttatttccc cgagaagct caattttgc gtaacgatt 
        ttatttatc aatatgttt gtaacaaaa ctactaata 
        aagtgtaat aaatacg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY