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Gene information
General information
TC030319 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13099980..13103170
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030319
    iBeetle screen iB_09501

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030319 RA
      MMSDEYVKD VFIANRWML RCAGLWTPS TRSKLVQIP 
      YKIYAIVVF LFVNVYFTS TEFLSLFYT HKNLYNFIK 
      NVNFFLTHF MGAVKVIFW FFKGHVLRD LMRTLESPE 
      FHYEPCEGF QPGLIWRKY RRIGFKYSL GFLALAHMT 
      LSSSYIPPL LTVTLNPPP YQNGTLSPF YQKLPYFSW 
      MPFSYSTPR SYLLALGYQ AGPMFSYAY SIVGMDTLF 
      MNIMNFIAA HLVILQGAF ASSKMRVLD PGQMNNEMK 
      RNCRHLQTI LRVSEDLER VHRYLTLGQ LTATLFILC 
      TSLYLISTT PASSKQFYA ELVYMVAMG FQLYLYCWF 
      GNEVTLMAS EIPVNVWKA DWYDCDQSF KKSMIFTMT 
      RMQKPIYMT VGKFAPLTL QTFVYILRT SYSIFAVIK 
      NTSI
    CDS sequence
    • >TC030319 RA
      atgatgtca gacgagtac gtcaaagac gtgtttata 
      gcaaatcgg tggatgttg cgttgtgct ggtctttgg 
      acaccaagc actcgctca aaactcgtc caaatccct 
      tacaaaatc tacgcaatt gttgttttt ctcttcgtc 
      aatgtttac ttcacttcg acggaattc ctaagtttg 
      ttctacacc cacaaaaac ttgtacaac tttatcaag 
      aacgtgaac tttttcctg acccacttc atgggggcc 
      gtgaaggtg attttttgg ttcttcaaa gggcacgtt 
      ttaagggac ttgatgcga acgttggaa agccccgag 
      ttccactac gagccctgt gaagggttc cagcctggg 
      ctaatttgg aggaagtac cgccggatc ggcttcaag 
      tactcccta gggttcctg gctcttgcc catatgacc 
      ctttcctcg tcctacatc ccccctttg ctcacggtg 
      actctgaac ccgccacct taccaaaat ggtactctc 
      agtcctttt taccagaaa ctgccctat tttagctgg 
      atgcccttt tcgtactcc acccccagg tcgtatctg 
      ctggccctg gggtaccag gctgggcct atgttctcc 
      tatgcttac agtatagtt ggaatggat acgcttttt 
      atgaatatt atgaatttt attgctgct catttggtc 
      attttgcaa ggggctttt gcaagcagt aaaatgcgg 
      gttttggac ccggggcag atgaacaat gagatgaaa 
      aggaattgc cgacacttg cagacaata ctcagggtt 
      tccgaggac cttgaaagg gtgcacagg tacttaact 
      ttgggacaa ctaactgca acattgttc atactttgc 
      acctccttg tatttaata tcgacaacc ccagctagt 
      agtaagcaa ttttacgcc gaattggtc tacatggta 
      gccatgggt ttccaatta tatctctac tgttggttt 
      ggtaacgaa gtgactttg atggctagt gagattcct 
      gtaaacgta tggaaggcc gattggtac gactgcgac 
      caatcgttc aagaaaagc atgattttt accatgact 
      cgaatgcaa aaaccgatt tatatgact gtggggaaa 
      ttcgcacct ttaacacta caaactttc gtctacatt 
      ctcagaact tcatattct attttcgca gtgatcaaa 
      aatacaagt atttaa
    mRNA sequence
    • >TC030319 RA
      cgtaaaagg gggccaaaa aatagtaaa aatgatgtc 
      agacgagta cgtcaaaga cgtgtttat agcaaatcg 
      gtggatgtt gcgttgtgc tggtctttg gacaccaag 
      cactcgctc aaaactcgt ccaaatccc ttacaaaat 
      ctacgcaat tgttgtttt tctcttcgt caatgttta 
      cttcacttc gacggaatt cctaagttt gttctacac 
      ccacaaaaa cttgtacaa ctttatcaa gaacgtgaa 
      ctttttcct gacccactt catgggggc cgtgaaggt 
      gattttttg gttcttcaa agggcacgt tttaaggga 
      cttgatgcg aacgttgga aagccccga gttccacta 
      cgagccctg tgaagggtt ccagcctgg gctaatttg 
      gaggaagta ccgccggat cggcttcaa gtactccct 
      agggttcct ggctcttgc ccatatgac cctttcctc 
      gtcctacat ccccccttt gctcacggt gactctgaa 
      cccgccacc ttaccaaaa tggtactct cagtccttt 
      ttaccagaa actgcccta ttttagctg gatgccctt 
      ttcgtactc cacccccag gtcgtatct gctggccct 
      ggggtacca ggctgggcc tatgttctc ctatgctta 
      cagtatagt tggaatgga tacgctttt tatgaatat 
      tatgaattt tattgctgc tcatttggt cattttgca 
      aggggcttt tgcaagcag taaaatgcg ggttttgga 
      cccggggca gatgaacaa tgagatgaa aaggaattg 
      ccgacactt gcagacaat actcagggt ttccgagga 
      ccttgaaag ggtgcacag gtacttaac tttgggaca 
      actaactgc aacattgtt catactttg cacctcctt 
      gtatttaat atcgacaac cccagctag tagtaagca 
      attttacgc cgaattggt ctacatggt agccatggg 
      tttccaatt atatctcta ctgttggtt tggtaacga 
      agtgacttt gatggctag tgagattcc tgtaaacgt 
      atggaaggc cgattggta cgactgcga ccaatcgtt 
      caagaaaag catgatttt taccatgac tcgaatgca 
      aaaaccgat ttatatgac tgtggggaa attcgcacc 
      tttaacact acaaacttt cgtctacat tctcagaac 
      ttcatattc tattttcgc agtgatcaa aaatacaag 
      tatttaaag caaaataaa tcatatctc gagtctaat 
      ggttttgtt tgtggtcaa aaatgaccg tcgctgctg 
      gatactagt ctccaggct ataattttt cttgtttgg 
      ataaatatt ttgcaccct ggggtttat tttcgatgg 
      gtttattag ggccaagtg atttttctg agtcaactt 
      tggtattta gccacttgt tttgacgtg ttctagatt 
      agaaagtgt gtttagtct gtggccgca cgatgcaaa 
      ttgcatcaa aataactaa aaaattcgg tttatttat 
      gaaaactga tggaacttg gttagttgt aacagaaaa 
      aacgtaaat agataaaag cggattagg taattttta 
      ttttaattg ttgcggttc tggaacaaa tatcactga 
      tagcttttt gtggtagat aattattat tcataatga 
      tgttaacat aacaataaa tgagtaaga aacct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY