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Gene information
General information
TC011841 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12685828..12689664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011841
    iBeetle screen iB_09500

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011841 RA
      MAFNPLTEA ECGQKNPLA RLTSHLTHD HAFAEAHGH 
      AFPSSSDQL VEQFLQETK SVPQSFRMD DLMREMHEI 
      ESQRSALPP IPASTIKDQ IHDDAWAQQ YIDDGKIFN 
      DNVNSEAIW TEILSQEQN NAAVVDDWG TDNFPEILP 
      GPSKVNASQ QSAREFVEA VDDPKFVYS KFMRFMKQV 
      GDGDVNLED GRLTEEEAQ SWSKEFLQN NNEAEAEKV 
      AKKWEKDYE DDFNSKYWN NLQEELKQT LEGAESSEN 
      TWASEFDSY YTAPYNDYT FTEENPMFD IPDPLQRGK 
      KLLEDGDLP SAVLCFEAA VKQEPENSE AWLLLGKTQ 
      AENEQDCNA IPALKKCIE LEPNNLTAL MALAVCYTN 
      ESYYSQAAQ MLLKWLTNN PKYSDLVPP DFQLTGQVT 
      SFLQPNQQR FIQDLYIKA ALRQPVDID YEVQCGLGV 
      LFNLSGEYE KAADCFRAA LSVKYDDAK LWNRLGATL 
      ANGSKSEEA VEAYHRALD LEPGFIRAR YNVGIICIN 
      LRAYREAAE HFLTALNQQ ARGRDVKNS PAMSQMSDT 
      IWSTLRMCI SLLNKVELR AAVDNRDLE TLNKAFDIV
    CDS sequence
    • >TC011841 RA
      atggcattt aaccccttg accgaggcc gagtgcggc 
      cagaagaac cccctggcc cgcctgacg tcgcacctg 
      acccacgat catgccttc gcggaggcc cacgggcat 
      gccttcccc agcagctcg gaccaactg gtcgagcag 
      ttcctgcaa gagacgaag tcggtgccc cagagcttc 
      cggatggac gatttgatg cgagaaatg cacgaaatt 
      gagtctcaa aggtcggcg ctgccgcca attcccgcc 
      tccacgatc aaggaccag atccacgac gacgcctgg 
      gcgcagcag tacatcgac gacgggaaa atcttcaat 
      gacaatgtg aacagtgag gcgatctgg acggagatc 
      ctctcgcag gagcagaac aatgcggct gtggtggac 
      gactggggc actgataat ttcccagag atactgcca 
      gggcccagc aaggtaaat gcctcgcag cagagcgct 
      cgggagttt gttgaggct gttgatgac cccaaattt 
      gtgtattcg aagtttatg agattcatg aaacaagtc 
      ggcgatggt gatgttaat ttggaggac gggaggctg 
      acggaggaa gaggcgcag tcctggagc aaggagttc 
      ctccaaaat aacaacgaa gcggaggct gaaaaagtc 
      gccaaaaag tgggaaaag gattacgaa gacgatttc 
      aattccaaa tactggaac aacctccag gaagagctc 
      aaacaaact ctagaaggc gccgaatct agcgaaaac 
      acttgggcg agcgaattc gactcctac tatactgcc 
      ccatacaac gactatacc tttaccgaa gaaaatccc 
      atgtttgat attcctgac cctttacaa agaggcaaa 
      aaactgctc gaagacggc gatctgccg tcggctgtt 
      ttatgtttc gaagcggcc gtaaaacaa gagcctgaa 
      aacagcgaa gcttggcta ctattaggc aaaacccaa 
      gcggaaaac gaacaggac tgtaatgcc ataccggca 
      ttgaaaaag tgtatagaa ctggaaccc aataattta 
      acggcgctc atggccttg gctgtctgt tacaccaac 
      gagagttac tacagtcag gctgcacaa atgttgctc 
      aaatggctc acaaataac ccgaaatat agcgatctg 
      gtgccaccc gattttcaa ctaactggg caagttaca 
      agcttttta cagccgaac cagcagcga ttcatccag 
      gacttatac ataaaagcc gctttacga caacctgtg 
      gatattgat tacgaagta cagtgtggt ctcggcgtt 
      ttgttcaat ttatctgga gagtacgaa aaggcggct 
      gattgtttc cgagccgcg ctgtccgta aaatacgac 
      gacgccaaa ttgtggaac cgtttgggg gcgacgtta 
      gccaacggg tccaaatcg gaggaggct gtggaggcg 
      tatcaccgc gcactagac ctagagcct ggttttata 
      cgagcgcgg tataatgta ggaataatt tgtataaat 
      ttaagggcc tatagggag gccgccgaa cacttttta 
      actgctctc aaccaacag gcgaggggc agggacgta 
      aaaaattca cccgcaatg tcgcagatg tcggacacg 
      atatggtcc acgttgcga atgtgtatc tctttgttg 
      aataaggtg gaacttagg gcggcagtg gataatcgg 
      gacttggaa acgctgaat aaggctttc gatattgtg 
      tag
    mRNA sequence
    • >TC011841 RA
      tcagtgtca cttacgtca atctggaat ttgttttga 
      taaaatgca cttggttgt gtaaactgg gctaaaaac 
      cgaaaaaat ggcatttaa ccccttgac cgaggccga 
      gtgcggcca gaagaaccc cctggcccg cctgacgtc 
      gcacctgac ccacgatca tgccttcgc ggaggccca 
      cgggcatgc cttccccag cagctcgga ccaactggt 
      cgagcagtt cctgcaaga gacgaagtc ggtgcccca 
      gagcttccg gatggacga tttgatgcg agaaatgca 
      cgaaattga gtctcaaag gtcggcgct gccgccaat 
      tcccgcctc cacgatcaa ggaccagat ccacgacga 
      cgcctgggc gcagcagta catcgacga cgggaaaat 
      cttcaatga caatgtgaa cagtgaggc gatctggac 
      ggagatcct ctcgcagga gcagaacaa tgcggctgt 
      ggtggacga ctggggcac tgataattt cccagagat 
      actgccagg gcccagcaa ggtaaatgc ctcgcagca 
      gagcgctcg ggagtttgt tgaggctgt tgatgaccc 
      caaatttgt gtattcgaa gtttatgag attcatgaa 
      acaagtcgg cgatggtga tgttaattt ggaggacgg 
      gaggctgac ggaggaaga ggcgcagtc ctggagcaa 
      ggagttcct ccaaaataa caacgaagc ggaggctga 
      aaaagtcgc caaaaagtg ggaaaagga ttacgaaga 
      cgatttcaa ttccaaata ctggaacaa cctccagga 
      agagctcaa acaaactct agaaggcgc cgaatctag 
      cgaaaacac ttgggcgag cgaattcga ctcctacta 
      tactgcccc atacaacga ctatacctt taccgaaga 
      aaatcccat gtttgatat tcctgaccc tttacaaag 
      aggcaaaaa actgctcga agacggcga tctgccgtc 
      ggctgtttt atgtttcga agcggccgt aaaacaaga 
      gcctgaaaa cagcgaagc ttggctact attaggcaa 
      aacccaagc ggaaaacga acaggactg taatgccat 
      accggcatt gaaaaagtg tatagaact ggaacccaa 
      taatttaac ggcgctcat ggccttggc tgtctgtta 
      caccaacga gagttacta cagtcaggc tgcacaaat 
      gttgctcaa atggctcac aaataaccc gaaatatag 
      cgatctggt gccacccga ttttcaact aactgggca 
      agttacaag ctttttaca gccgaacca gcagcgatt 
      catccagga cttatacat aaaagccgc tttacgaca 
      acctgtgga tattgatta cgaagtaca gtgtggtct 
      cggcgtttt gttcaattt atctggaga gtacgaaaa 
      ggcggctga ttgtttccg agccgcgct gtccgtaaa 
      atacgacga cgccaaatt gtggaaccg tttgggggc 
      gacgttagc caacgggtc caaatcgga ggaggctgt 
      ggaggcgta tcaccgcgc actagacct agagcctgg 
      ttttatacg agcgcggta taatgtagg aataatttg 
      tataaattt aagggccta tagggaggc cgccgaaca 
      ctttttaac tgctctcaa ccaacaggc gaggggcag 
      ggacgtaaa aaattcacc cgcaatgtc gcagatgtc 
      ggacacgat atggtccac gttgcgaat gtgtatctc 
      tttgttgaa taaggtgga acttagggc ggcagtgga 
      taatcggga cttggaaac gctgaataa ggctttcga 
      tattgtgta gaattgaga ttgtgcaga ttgttattt 
      ttgtatgcc ccaaaaaat atactgttg gtcttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY