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Gene information
General information
TC011864 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12431654..12432895
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011864
    iBeetle screen iB_09496

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011864 RA
      MMSCGELFL KGASSTFTG YFLIVSIAF 
      LLYGLTTTY YLLKSNTAE TLEEELVSL 
      PSLCLILFG FLLFLFIIL GCCGICREI 
      PCCLETYAI FLALLAAAQ LGVGIFALV 
      NFDPSNEDF QKRIHDDVG KMFEIYYNN 
      RTQLDNTQQ WMKCCGVTD DGPSDWLQN 
      RTIPSSCCE NSYQNCTIF SESIYHDGC 
      GQTLYFYIV GTNYIVGLA LVVTAVTEL 
      IASVMGIGL SCAFWRNSR VMWI
    CDS sequence
    • >TC011864 RA
      atgatgagt tgcggagag ctgttcctc aaaggtgcc 
      agcagcact ttcaccgga tatttcctc atagtcagc 
      atagcgttc ctcctgtac ggcctcacg accacgtac 
      tacctcctg aaaagcaac actgctgaa accctggag 
      gaagaacta gtctcactg ccctcactg tgcctcatc 
      ctctttggc ttcctcctc ttcctcttc ataatcttg 
      ggctgctgt gggatttgt cgggaaatc ccctgctgt 
      cttgaaact tacgctatt ttcctcgcc ttattagcc 
      gcagctcag ctcggcgta ggaattttc gctttagtg 
      aattttgac ccaagcaat gaagatttt caaaaacgc 
      atacatgac gatgtggga aaaatgttt gaaatttac 
      tacaacaac cgcacacaa ttggacaac acccagcaa 
      tggatgaaa tgttgcgga gttacagac gatggacca 
      agtgactgg ttacaaaat cgtacaatt ccgtcatca 
      tgttgcgaa aattcgtac cagaattgt accattttc 
      tcagagagt atttatcac gatgggtgc ggacagacg 
      ctttatttt tacattgtt gggactaat tacattgtt 
      gggttggcc cttgtagtg acggccgtt actgagctg 
      attgcctct gtgatgggg atcggattg tcttgtgcc 
      ttttggagg aacagcaga gttatgtgg atttaa
    mRNA sequence
    • >TC011864 RA
      tcagcacgt ttcagtctt ggtggcgcc gcctcggcc 
      gaaaacatt cgatttaaa aataaaatg cgtcgggtt 
      gtctctgca acttttcga tgatgagtt gcggagagc 
      tgttcctca aaggtgcca gcagcactt tcaccggat 
      atttcctca tagtcagca tagcgttcc tcctgtacg 
      gcctcacga ccacgtact acctcctga aaagcaaca 
      ctgctgaaa ccctggagg aagaactag tctcactgc 
      cctcactgt gcctcatcc tctttggct tcctcctct 
      tcctcttca taatcttgg gctgctgtg ggatttgtc 
      gggaaatcc cctgctgtc ttgaaactt acgctattt 
      tcctcgcct tattagccg cagctcagc tcggcgtag 
      gaattttcg ctttagtga attttgacc caagcaatg 
      aagattttc aaaaacgca tacatgacg atgtgggaa 
      aaatgtttg aaatttact acaacaacc gcacacaat 
      tggacaaca cccagcaat ggatgaaat gttgcggag 
      ttacagacg atggaccaa gtgactggt tacaaaatc 
      gtacaattc cgtcatcat gttgcgaaa attcgtacc 
      agaattgta ccattttct cagagagta tttatcacg 
      atgggtgcg gacagacgc tttattttt acattgttg 
      ggactaatt acattgttg ggttggccc ttgtagtga 
      cggccgtta ctgagctga ttgcctctg tgatgggga 
      tcggattgt cttgtgcct tttggagga acagcagag 
      ttatgtgga tttaagcgc tgttaaggc ggagatacg 
      ggttattta taaataaaa agtgtattt attt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY