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Gene information
General information
TC011873 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12343350..12348825
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011873
    iBeetle screen iB_09495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs potentially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011873 RA
      MLRIILSLF SLIICSNCF NIDTNFPIV YQDTQSSSY 
      FGYSTILYP GNQRYPPWL LVGAPKATN PFTTYKNPG 
      ACFKCVIGD KCHIFNFTD ASTSPPRYE EKLDGAWIG 
      GSMDISYKH GRVVVCAPR WIKIKKKNG KYDDFHMEG 
      ACYWFHTND NTTQKLLPL FEPDHAVYS SGGGAVYNY 
      AQGAAGISA HMPVKEKHL VLGAPGVFN WDGTSILYT 
      DGDNPSPVA SRLRNKVKR YADIDEFSH SNVANAMLT 
      SQTVAYDLL GYATTSGYF YRKSQLLYV TAAPRSDYK 
      GQILIYKFE EQRERPLDV KETRFGHQF GEYFGGSLT 
      AGDVNGDGL DDLVVGAPF HTTGKFNEG KVYIFLGSG 
      SGSLRVPKR ESILGGSTN GQFGSTVQF LGDVDHDGF 
      GDVAVAAPY EESKSGVVY LYRGSSDGL ITTPSQRIV 
      GKNIYPEIR GFGVSISRP ADIDTNSYP DIAIGAYLS 
      GHAVVLRSR PVVTIYQEL KSFSQHLDF KEKRFKVRA 
      CFWYRLDEL DPPLISITR YLLVDQDLG RAHLEGNSV 
      KTISLNQNR QHCENFTIT LQDSFSNRY NPITVSLKH 
      NFLIRPETK KILLVGSNS LSDDMFCPR CPVYNKHDS 
      VSSSIIEIP FALGCGPDN ICKASLEFT SHLIDFGNT 
      EEFILGSKN YITLETVVR NLGERAYLT QLEVEIPKI 
      VTLRSIPST CAQQNSSVL CSVDNPLET NSLKKITLE 
      LDMTTVNSG KYDDFLNFT IKIFTSSEN DQENVKQLV 
      VKLGRRADV TITGKSEEQ SYSYKNLTQ GRSFAQTYQ 
      VQKFGSSPL NEISIEINV PHILGHEIF INLYQPVGT 
      LSGQKLTCS SNFTYIVDN NQEFGVLED DKVRSKRSL 
      SQIEVFNKS AKVRERITD STFDALQNR TFYINCSTP 
      GVTCSRVVC SGGPIDSYQ TATLKLQMF FNTSAIKDL 
      FGKKDVILF ATQGRVLVT SPANFVQSG GRTDNFDVV 
      SLFIGETIE EKVELWIII ISVIAGLLL LLLVILGLI 
      KAGFFKRTK KEELEQLRL EMEFNQGGH SNTMETLDD 
      GETEEEIS
    CDS sequence
    • >TC011873 RA
      atgctgcga attattctc tcccttttt tcactcatt 
      atttgcagt aattgtttc aacattgat acaaatttc 
      ccaatagtg taccaagac acacaaagc tcaagctat 
      ttcggctat tcgacaatt ctatatcct ggaaatcaa 
      agatacccc ccttggctt ttagtcggc gccccaaaa 
      gccaccaac cctttcacc acttacaaa aacccggga 
      gcttgtttc aagtgcgtt attggcgat aaatgccac 
      atttttaat ttcactgat gcctccaca tctcctcca 
      cgttatgag gaaaagtta gatggtgcc tggatcggg 
      ggatcgatg gacataagt tacaaacac ggccgagtc 
      gttgtctgt gccccgcgc tggatcaaa ataaagaag 
      aagaacggc aaatacgac gattttcac atggagggg 
      gcatgttat tggttccac accaacgac aatacgacg 
      caaaaattg ttaccactg tttgaaccc gatcacgct 
      gtgtactcg tctgggggc ggcgctgtg tacaattac 
      gcgcaagga gcggccgga atttccgct cacatgccg 
      gtgaaagaa aaacacctg gttctgggg gccccgggc 
      gtcttcaac tgggacggg acgtcgatt ttgtacaca 
      gacggcgac aatccgtct cctgtagcc agtcgtttg 
      cgaaacaaa gtgaaaaga tacgccgac attgacgaa 
      ttcagtcac tccaatgtg gcaaatgct atgctaact 
      tcccagact gtggcttac gatttgctg ggctatgcc 
      acaacgtcg ggttatttc taccgaaag tcgcagctt 
      ttgtacgtc acagcggcc ccaagatcg gactataaa 
      ggccaaatt ttgatttac aaattcgaa gaacaacgt 
      gaaaggccg ttggatgtg aaagaaacg aggtttggt 
      caccaattt ggggagtat tttggcggt tcgctgact 
      gcaggggat gtaaatggt gatgggttg gatgatttg 
      gtcgtgggg gccccattt cataccact gggaagttt 
      aacgaaggg aaagtttac attttctta gggagtggt 
      tcgggttct ttacgagtc cccaaacga gagtccatc 
      ctcggaggg agtaccaat ggccagttt gggagcacg 
      gtgcagttt ttgggggac gtggaccat gatgggttt 
      ggagatgtg gcagtggct gccccttac gaagagtcg 
      aaaagcggt gttgtttac ctatacaga ggttcaagt 
      gatggttta ataacaact cctagtcaa agaattgtt 
      ggtaaaaat atttatccc gaaatacga ggctttgga 
      gtgagtata tcacgtcca gcggatatt gataccaac 
      agctaccca gatattgca attggtgcg tatttatcg 
      ggccatgct gtagtttta cgttcacgt ccagtggtc 
      acaatttac caagagtta aaatcgttc tcacaacat 
      ttggatttt aaggaaaaa cggttcaaa gtacgggct 
      tgtttctgg tatagactg gacgaacta gacccgcct 
      ctaatatca atcacgagg tatttattg gttgatcaa 
      gatttggga agagcccac ttggaagga aattccgtt 
      aaaaccatt agtttgaat cagaatcga caacactgc 
      gaaaatttc actataaca cttcaagat tcgttttca 
      aatcgttat aacccgatt acagtctct ttgaaacac 
      aattttctg attagacct gagacgaaa aaaattctt 
      ttggtcggt tctaattcc ctatcagat gatatgttt 
      tgtcccaga tgccccgtc tacaacaaa cacgattcg 
      gtttcttca agcataatc gaaattccc ttcgctttg 
      ggttgtggc cctgataat atttgcaaa gcgagttta 
      gagtttaca tcacactta attgatttc gggaacacc 
      gaggaattt attttgggc tcgaaaaac tacataact 
      ctagaaact gtggtaaga aatttaggc gaacgggcg 
      tatttgaca caattggaa gtggaaata cccaaaatt 
      gtgactctc cgaagcata ccgtcgact tgtgcccaa 
      cagaattcg tcggttttg tgttccgtc gataatcct 
      ctggaaact aacagtctg aagaaaatt acgctagaa 
      ttggatatg acgactgtg aatagcgga aaatacgac 
      gatttcctg aatttcacg atcaaaata ttcaccagc 
      agtgaaaat gaccaagaa aatgttaaa caactcgtt 
      gtaaaactc ggtcgaaga gctgatgtt actattact 
      ggtaaatcg gaggagcaa agctactcg tataaaaac 
      ctaacacaa gggcggtct ttcgcccaa acctaccag 
      gtccagaaa tttggaagc agtcctttg aacgaaata 
      tctatcgaa attaatgtt ccccatatt ttggggcat 
      gaaattttt atcaatttg taccaaccg gtgggaact 
      ctatccggc caaaaactc acgtgttcg agtaatttc 
      acgtacatt gtggacaat aatcaagaa tttggcgtc 
      ctggaggac gataaagtt cgctcaaaa cgttcctta 
      tcccaaatt gaagttttc aacaaatcg gcgaaagtt 
      agggaaaga attacagac agcactttt gacgcgtta 
      caaaatcgc actttttac atcaactgt tcaacacct 
      ggagtgact tgttcaagg gttgtgtgc tccggtggt 
      cctattgat tcctaccag acggcaaca ctaaaattg 
      caaatgttt tttaacacc agtgcaatc aaagatttg 
      tttggcaaa aaggatgtg atcctgttt gcgactcaa 
      gggcgggtt ctggtgaca agtcccgcg aattttgtg 
      caaagtggc ggcaggact gataatttt gatgtggtt 
      tcgttattt attggggag acgattgag gaaaaggtg 
      gagctttgg atcattatt atctctgtt attgcgggt 
      ttattgcta cttttgctg gtcattctg ggccttatt 
      aaagccgga tttttcaaa agaactaag aaggaggaa 
      ttggaacag ctcaggctg gagatggaa ttcaaccaa 
      ggtgggcat tcaaatacg atggagacc ctagacgac 
      ggagaaacg gaggaagaa atttcataa
    mRNA sequence
    • >TC011873 RA
      aaagacaag aaacgaaaa tgctgcgaa ttattctct 
      ccctttttt cactcatta tttgcagta attgtttca 
      acattgata caaatttcc caatagtgt accaagaca 
      cacaaagct caagctatt tcggctatt cgacaattc 
      tatatcctg gaaatcaaa gataccccc cttggcttt 
      tagtcggcg ccccaaaag ccaccaacc ctttcacca 
      cttacaaaa acccgggag cttgtttca agtgcgtta 
      ttggcgata aatgccaca tttttaatt tcactgatg 
      cctccacat ctcctccac gttatgagg aaaagttag 
      atggtgcct ggatcgggg gatcgatgg acataagtt 
      acaaacacg gccgagtcg ttgtctgtg ccccgcgct 
      ggatcaaaa taaagaaga agaacggca aatacgacg 
      attttcaca tggaggggg catgttatt ggttccaca 
      ccaacgaca atacgacgc aaaaattgt taccactgt 
      ttgaacccg atcacgctg tgtactcgt ctgggggcg 
      gcgctgtgt acaattacg cgcaaggag cggccggaa 
      tttccgctc acatgccgg tgaaagaaa aacacctgg 
      ttctggggg ccccgggcg tcttcaact gggacggga 
      cgtcgattt tgtacacag acggcgaca atccgtctc 
      ctgtagcca gtcgtttgc gaaacaaag tgaaaagat 
      acgccgaca ttgacgaat tcagtcact ccaatgtgg 
      caaatgcta tgctaactt cccagactg tggcttacg 
      atttgctgg gctatgcca caacgtcgg gttatttct 
      accgaaagt cgcagcttt tgtacgtca cagcggccc 
      caagatcgg actataaag gccaaattt tgatttaca 
      aattcgaag aacaacgtg aaaggccgt tggatgtga 
      aagaaacga ggtttggtc accaatttg gggagtatt 
      ttggcggtt cgctgactg caggggatg taaatggtg 
      atgggttgg atgatttgg tcgtggggg ccccatttc 
      ataccactg ggaagttta acgaaggga aagtttaca 
      ttttcttag ggagtggtt cgggttctt tacgagtcc 
      ccaaacgag agtccatcc tcggaggga gtaccaatg 
      gccagtttg ggagcacgg tgcagtttt tgggggacg 
      tggaccatg atgggtttg gagatgtgg cagtggctg 
      ccccttacg aagagtcga aaagcggtg ttgtttacc 
      tatacagag gttcaagtg atggtttaa taacaactc 
      ctagtcaaa gaattgttg gtaaaaata tttatcccg 
      aaatacgag gctttggag tgagtatat cacgtccag 
      cggatattg ataccaaca gctacccag atattgcaa 
      ttggtgcgt atttatcgg gccatgctg tagttttac 
      gttcacgtc cagtggtca caatttacc aagagttaa 
      aatcgttct cacaacatt tggatttta aggaaaaac 
      ggttcaaag tacgggctt gtttctggt atagactgg 
      acgaactag acccgcctc taatatcaa tcacgaggt 
      atttattgg ttgatcaag atttgggaa gagcccact 
      tggaaggaa attccgtta aaaccatta gtttgaatc 
      agaatcgac aacactgcg aaaatttca ctataacac 
      ttcaagatt cgttttcaa atcgttata acccgatta 
      cagtctctt tgaaacaca attttctga ttagacctg 
      agacgaaaa aaattcttt tggtcggtt ctaattccc 
      tatcagatg atatgtttt gtcccagat gccccgtct 
      acaacaaac acgattcgg tttcttcaa gcataatcg 
      aaattccct tcgctttgg gttgtggcc ctgataata 
      tttgcaaag cgagtttag agtttacat cacacttaa 
      ttgatttcg ggaacaccg aggaattta ttttgggct 
      cgaaaaact acataactc tagaaactg tggtaagaa 
      atttaggcg aacgggcgt atttgacac aattggaag 
      tggaaatac ccaaaattg tgactctcc gaagcatac 
      cgtcgactt gtgcccaac agaattcgt cggttttgt 
      gttccgtcg ataatcctc tggaaacta acagtctga 
      agaaaatta cgctagaat tggatatga cgactgtga 
      atagcggaa aatacgacg atttcctga atttcacga 
      tcaaaatat tcaccagca gtgaaaatg accaagaaa 
      atgttaaac aactcgttg taaaactcg gtcgaagag 
      ctgatgtta ctattactg gtaaatcgg aggagcaaa 
      gctactcgt ataaaaacc taacacaag ggcggtctt 
      tcgcccaaa cctaccagg tccagaaat ttggaagca 
      gtcctttga acgaaatat ctatcgaaa ttaatgttc 
      cccatattt tggggcatg aaattttta tcaatttgt 
      accaaccgg tgggaactc tatccggcc aaaaactca 
      cgtgttcga gtaatttca cgtacattg tggacaata 
      atcaagaat ttggcgtcc tggaggacg ataaagttc 
      gctcaaaac gttccttat cccaaattg aagttttca 
      acaaatcgg cgaaagtta gggaaagaa ttacagaca 
      gcacttttg acgcgttac aaaatcgca ctttttaca 
      tcaactgtt caacacctg gagtgactt gttcaaggg 
      ttgtgtgct ccggtggtc ctattgatt cctaccaga 
      cggcaacac taaaattgc aaatgtttt ttaacacca 
      gtgcaatca aagatttgt ttggcaaaa aggatgtga 
      tcctgtttg cgactcaag ggcgggttc tggtgacaa 
      gtcccgcga attttgtgc aaagtggcg gcaggactg 
      ataattttg atgtggttt cgttattta ttggggaga 
      cgattgagg aaaaggtgg agctttgga tcattatta 
      tctctgtta ttgcgggtt tattgctac ttttgctgg 
      tcattctgg gccttatta aagccggat ttttcaaaa 
      gaactaaga aggaggaat tggaacagc tcaggctgg 
      agatggaat tcaaccaag gtgggcatt caaatacga 
      tggagaccc tagacgacg gagaaacgg aggaagaaa 
      tttcataat tttgtattc atttaattg caattagtg 
      ctaatttta attttatta aaaattaaa ttaaataaa 
      aatatccga taata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY