Status
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Gene information
General information
TC011312 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3156774..3167628
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011312
    iBeetle screen iB_09494

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011312 RA
      MEDSLSVKS NESVATSEE FDFVGDKPA NKTPTLDIK 
      GDLSELKSA LTEVLQESG RNMDEVDGK EKKIGHSTF 
      YGCLVEPGK DPLSRTGED SPGEKQDSV KQDSNEEEE 
      VSDIDQDCT IFAGVTYLG AAAINAPKS EAEIQRNMT 
      ILNEQSSEQ GIKVSVSVP CSSQGFVVL YDANSNSVI 
      SRYEIHRIL FYARGAIDG SEAGCFAFT WSHGDTQES 
      AIFQCHVFR CDIAEAVSR VSACFAKAF QRVPRSMSS 
      SVTSALDMT GSITTIPEA RTLMYVFEV SLEIKEDDG 
      KGQLSTVGK DRSGFKLKT NLEKQLCLS IRQVSDNGP 
      QLHIERCFG VLVAPGRHV RHSDMQLLE MKEQVPSNN 
      SCTLISATW DPTEPSFES LNQETPKDV KQFMTVAVD 
      LVIRGIQEP VRFQIETPV RIFGQNERN FWYFQKRSL 
      VQQFFLNLK EVVAGDLND IYYEVLNME TSGELDRNL 
      LNLNLNLSS LIQSPSITS IDTITPKDE YNSDGDEPL 
      LSGTGLVSK DCSEDVLES WAEVLNRWK STKQPPKQL 
      ASLVRTGIP EALRGEVWQ RLAGVEEDT EMMENYRLL 
      ITKESSCEN VIQRDIART FPAHDFFKE AGGLGQDSL 
      YRVSKAYAV YDSEVGYCQ GLSFLAATL LLHMPEEQA 
      FCVLVKLMY NYHLRDLYK DGFDNLYLR LYQLNCLMK 
      EQLSPLWQH FADHHVETH MFASQWFLT LFTARFPLN 
      FVFQIIDVF LLQGIDTLF QVALALLTA YAKDLLQLD 
      FEGILKYFR VSLPKKCRN EDATRQLIK SACMIKLKK 
      LRKFEAEYF ALKEAADNA ESEANEVQQ LRVTLGRYE 
      DERKLMFDE IAQLKDMLK REVEQAEKD KKNYTTIIN 
      DYKLVRQRL DTQLHAVRT ELENLKYKL SNCDNCKVL 
      IDEPKQYNE ETETDGSER VRELELELA QTKLAQVEA 
      ECRNQDLIH RLRTTEIEL TSAKNSWPP WLSKTLSSI 
      KEVANKKDF GNMTYPSNI PNTTPSFIS HINAIRRDS 
      VPLRSDHSN VREQRRDST PIIKDSQSC SSLKTHN
    CDS sequence
    • >TC011312 RA
      atggaggat agtttgagc gtgaaatca aatgagtcg 
      gtggcaacg agcgaagag tttgatttc gttggggac 
      aagcctgcg aacaagacg cccacgttg gatattaag 
      ggggatcta agcgagttg aagagtgcc ttgacggag 
      gtgctgcag gagtcgggg aggaatatg gacgaggtg 
      gacggcaag gagaagaag atcgggcat tcgacgttt 
      tatgggtgt ttggtggag ccggggaag gacccgctg 
      tcaaggacg ggggaggac agtcccggg gaaaaacag 
      gactcggtc aagcaggac tccaatgag gaggaggaa 
      gtttcagat atcgatcaa gattgtaca attttcgct 
      ggtgtgaca tatttgggc gccgccgcc ataaacgcc 
      cccaaatcg gaggccgaa atccaacga aacatgacc 
      atattaaac gaacaaagt tcagaacaa gggattaaa 
      gtctcggtt tcggtgcca tgctcatcg caggggttt 
      gtggttctg tacgacgcc aactcgaat tcggtgatt 
      tcccgctat gagatacac cgtattttg ttttatgct 
      cggggggcg atcgacggt agcgaagcc ggttgtttt 
      gcctttacg tggtcacat ggtgatacg caagaatcc 
      gccattttc cagtgccat gttttccgg tgtgacata 
      gccgaggcg gtgtcacgt gtctcagcg tgttttgcg 
      aaggcgttt caaagagtg ccacgctcg atgagcagt 
      tcggtcacg tcggctttg gatatgaca gggtccata 
      accacaata cccgaagcc aggacgctt atgtatgta 
      tttgaagtc agtttggag attaaggag gatgacggg 
      aagggacaa ctgtcgaca gttggtaaa gacaggtcc 
      gggtttaaa ttgaaaaca aatttggag aaacagctg 
      tgtttgagc atacggcag gtgtcggat aatgggccc 
      cagttgcat attgagcgt tgttttggg gtactggtg 
      gcccccgga cgacatgtg cgccactcc gacatgcaa 
      ctactcgaa atgaaagaa caagttccg tcgaataac 
      tcctgtact ttaatttcg gcgacttgg gatccgacc 
      gaaccttcg ttcgagtcg ttaaatcag gaaacaccg 
      aaagatgtg aaacaattc atgacagtc gctgtggat 
      ctagtgata cgtggaata caggaacct gtaaggttt 
      caaatcgaa acaccggtc cgtattttc ggacagaat 
      gagcgaaat ttctggtat tttcagaag aggtcgctc 
      gtgcaacag ttttttttg aatctcaaa gaggtggtg 
      gctggtgat ctcaacgac atatactat gaagttttg 
      aatatggaa acgtcgggt gaattggac aggaattta 
      cttaatctt aacttaaat ctatcgagc cttatacag 
      tcacctagt attacgtcc attgatacc ataacgcct 
      aaagatgag tacaattca gatggtgac gagcccctc 
      cttagcggc accggtctc gtgtcgaaa gactgctcc 
      gaagacgtc cttgaaagt tgggccgag gttttgaat 
      cgttggaag tcaacaaaa cagccacct aaacaactc 
      gccagtcta gtccgtacc ggaattccg gaagcttta 
      agaggtgaa gtatggcag cgtttagcc ggcgtcgaa 
      gaagacaca gaaatgatg gaaaattac agattactt 
      atcacaaaa gagagcagt tgcgagaat gtgatccaa 
      cgcgacatc gcacgcact ttcccggcc catgatttt 
      ttcaaagaa gccggtggt ctaggacaa gactctctc 
      tacagggtt agcaaagcc tacgccgtc tacgattca 
      gaagtcggc tattgtcag ggacttagt tttttagcg 
      gccacgtta ctgttgcac atgcctgag gaacaagct 
      ttctgtgtt ttagtcaaa ctgatgtac aattatcac 
      ttgagggat ttgtataaa gacggcttt gacaatctc 
      tacctacgc ctgtatcaa ctcaattgt cttatgaaa 
      gaacagttg tcgcctttg tggcaacac ttcgccgat 
      catcacgtg gaaacacac atgtttgca tcgcagtgg 
      tttctgacc ttatttact gccagattt cccctaaat 
      tttgttttc caaataatt gatgttttc ttgttgcaa 
      ggaatcgat actttgttt caagtcgct ttggctctc 
      ttaacggcg tatgcgaaa gatttgcta cagttggat 
      tttgaaggc attttgaaa tactttcgc gtttctctg 
      ccgaaaaaa tgtcgcaat gaggacgcc acaagacaa 
      ttgatcaag tcggcttgt atgataaaa ttgaagaag 
      ttgcgaaaa tttgaagcg gaatatttc gccctgaaa 
      gaagctgca gataacgcc gaaagtgaa gcgaacgaa 
      gtgcaacaa ttgcgtgtg acgttggga cggtacgaa 
      gatgaacgg aaattaatg tttgacgaa atcgcacag 
      ttgaaagat atgttgaaa cgcgaagtg gaacaagca 
      gagaaagat aaaaagaat tacacgaca atcataaac 
      gattataag ttggttaga caaaggttg gatactcag 
      ttgcacgca gtacggacc gaattggag aatctcaaa 
      tataaactg tcgaattgt gacaattgt aaggttctt 
      atcgacgaa ccgaaacag tacaacgaa gagaccgaa 
      actgacggc tccgaaagg gtgcgagaa ttggagtta 
      gagttggcg caaaccaag cttgcgcaa gtcgaagcc 
      gagtgtcgc aatcaggat ttgattcat cggttgcgc 
      acaaccgaa atcgagtta acttcggcc aaaaacagc 
      tggccaccg tggttgagt aagacgttg agttcgatc 
      aaagaagtt gccaataag aaagatttt ggcaatatg 
      acatatccc agcaacatt ccgaacaca acgccctct 
      tttatatca cacataaat gcaatacga cgcgatagc 
      gtgccattg aggagtgac catagtaat gtaagggag 
      cagagacgc gactcgacc ccgattatc aaggattct 
      cagagttgt agtagtttg aagactcac aattga
    mRNA sequence
    • >TC011312 RA
      ccaaaacaa aaaccgatc aaatgtcaa attcctgac 
      gtccacact tatttactc acatttcca cctcaaatc 
      gtctctcat ttcgcataa tgtgtttaa aagtgtcat 
      tttccctcc tggattaac tcacagtcg tggtgcacg 
      ccccgctcg tcgatgatg tagtggcag gttgcaagg 
      aggcgtaat aagatattg tgttgtctc gagtggccc 
      cacactatg gaggatagt ttgagcgtg aaatcaaat 
      gagtcggtg gcaacgagc gaagagttt gatttcgtt 
      ggggacaag cctgcgaac aagacgccc acgttggat 
      attaagggg gatctaagc gagttgaag agtgccttg 
      acggaggtg ctgcaggag tcggggagg aatatggac 
      gaggtggac ggcaaggag aagaagatc gggcattcg 
      acgttttat gggtgtttg gtggagccg gggaaggac 
      ccgctgtca aggacgggg gaggacagt cccggggaa 
      aaacaggac tcggtcaag caggactcc aatgaggag 
      gaggaagtt tcagatatc gatcaagat tgtacaatt 
      ttcgctggt gtgacatat ttgggcgcc gccgccata 
      aacgccccc aaatcggag gccgaaatc caacgaaac 
      atgaccata ttaaacgaa caaagttca gaacaaggg 
      attaaagtc tcggtttcg gtgccatgc tcatcgcag 
      gggtttgtg gttctgtac gacgccaac tcgaattcg 
      gtgatttcc cgctatgag atacaccgt attttgttt 
      tatgctcgg ggggcgatc gacggtagc gaagccggt 
      tgttttgcc tttacgtgg tcacatggt gatacgcaa 
      gaatccgcc attttccag tgccatgtt ttccggtgt 
      gacatagcc gaggcggtg tcacgtgtc tcagcgtgt 
      tttgcgaag gcgtttcaa agagtgcca cgctcgatg 
      agcagttcg gtcacgtcg gctttggat atgacaggg 
      tccataacc acaataccc gaagccagg acgcttatg 
      tatgtattt gaagtcagt ttggagatt aaggaggat 
      gacgggaag ggacaactg tcgacagtt ggtaaagac 
      aggtccggg tttaaattg aaaacaaat ttggagaaa 
      cagctgtgt ttgagcata cggcaggtg tcggataat 
      gggccccag ttgcatatt gagcgttgt tttggggta 
      ctggtggcc cccggacga catgtgcgc cactccgac 
      atgcaacta ctcgaaatg aaagaacaa gttccgtcg 
      aataactcc tgtacttta atttcggcg acttgggat 
      ccgaccgaa ccttcgttc gagtcgtta aatcaggaa 
      acaccgaaa gatgtgaaa caattcatg acagtcgct 
      gtggatcta gtgatacgt ggaatacag gaacctgta 
      aggtttcaa atcgaaaca ccggtccgt attttcgga 
      cagaatgag cgaaatttc tggtatttt cagaagagg 
      tcgctcgtg caacagttt tttttgaat ctcaaagag 
      gtggtggct ggtgatctc aacgacata tactatgaa 
      gttttgaat atggaaacg tcgggtgaa ttggacagg 
      aatttactt aatcttaac ttaaatcta tcgagcctt 
      atacagtca cctagtatt acgtccatt gataccata 
      acgcctaaa gatgagtac aattcagat ggtgacgag 
      cccctcctt agcggcacc ggtctcgtg tcgaaagac 
      tgctccgaa gacgtcctt gaaagttgg gccgaggtt 
      ttgaatcgt tggaagtca acaaaacag ccacctaaa 
      caactcgcc agtctagtc cgtaccgga attccggaa 
      gctttaaga ggtgaagta tggcagcgt ttagccggc 
      gtcgaagaa gacacagaa atgatggaa aattacaga 
      ttacttatc acaaaagag agcagttgc gagaatgtg 
      atccaacgc gacatcgca cgcactttc ccggcccat 
      gattttttc aaagaagcc ggtggtcta ggacaagac 
      tctctctac agggttagc aaagcctac gccgtctac 
      gattcagaa gtcggctat tgtcaggga cttagtttt 
      ttagcggcc acgttactg ttgcacatg cctgaggaa 
      caagctttc tgtgtttta gtcaaactg atgtacaat 
      tatcacttg agggatttg tataaagac ggctttgac 
      aatctctac ctacgcctg tatcaactc aattgtctt 
      atgaaagaa cagttgtcg cctttgtgg caacacttc 
      gccgatcat cacgtggaa acacacatg tttgcatcg 
      cagtggttt ctgacctta tttactgcc agatttccc 
      ctaaatttt gttttccaa ataattgat gttttcttg 
      ttgcaagga atcgatact ttgtttcaa gtcgctttg 
      gctctctta acggcgtat gcgaaagat ttgctacag 
      ttggatttt gaaggcatt ttgaaatac tttcgcgtt 
      tctctgccg aaaaaatgt cgcaatgag gacgccaca 
      agacaattg atcaagtcg gcttgtatg ataaaattg 
      aagaagttg cgaaaattt gaagcggaa tatttcgcc 
      ctgaaagaa gctgcagat aacgccgaa agtgaagcg 
      aacgaagtg caacaattg cgtgtgacg ttgggacgg 
      tacgaagat gaacggaaa ttaatgttt gacgaaatc 
      gcacagttg aaagatatg ttgaaacgc gaagtggaa 
      caagcagag aaagataaa aagaattac acgacaatc 
      ataaacgat tataagttg gttagacaa aggttggat 
      actcagttg cacgcagta cggaccgaa ttggagaat 
      ctcaaatat aaactgtcg aattgtgac aattgtaag 
      gttcttatc gacgaaccg aaacagtac aacgaagag 
      accgaaact gacggctcc gaaagggtg cgagaattg 
      gagttagag ttggcgcaa accaagctt gcgcaagtc 
      gaagccgag tgtcgcaat caggatttg attcatcgg 
      ttgcgcaca accgaaatc gagttaact tcggccaaa 
      aacagctgg ccaccgtgg ttgagtaag acgttgagt 
      tcgatcaaa gaagttgcc aataagaaa gattttggc 
      aatatgaca tatcccagc aacattccg aacacaacg 
      ccctctttt atatcacac ataaatgca atacgacgc 
      gatagcgtg ccattgagg agtgaccat agtaatgta 
      agggagcag agacgcgac tcgaccccg attatcaag 
      gattctcag agttgtagt agtttgaag actcacaat 
      tgatcgtaa attgatgtt attttattt tgttagtta 
      tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY