Status
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Gene information
General information
TC034408
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011884
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12248292..12252512
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034408
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09491

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034408 RA
      MSSKAKKGK KKPKSVNDV SKSKDTLTN KKIHAYFTN 
      EEIAEIKSI MKTKDTGVP FAIAATLLY KPDLLKKLL 
      KDGFNVNQT GQNNMTALM WAVKHKYLD IVDYLLTNG 
      ADPTIKTDS GDNVLILAL EHKLWDEQS MINLWKAVK 
      KVSFIDVNF TSKNGHNML HMCVRREWE QLLKLLLNE 
      KPDVDAGNS NGVTPLMLA CFRNNISII TTLINAGAD 
      ILKEDNHGR MTLCYAISS CMKVSKPPF PATEKIITE 
      LKNKSSLEN YLKRRLELI IATAKKSEI SCATAEMLI 
      KIVQFAVQY MEEGIPTFL ECDVFAQLL EIITSRLDN 
      SAIVKFLLE VCEEILFYN EQCVGYKVE LGNKLVEGF 
      YASGLVDNV LEVLRKQSE EAKIAFKVL LLGFNNDER 
      WEKTMRDNS DVLSPLLND PQNLDEDES RAKILKNKK 
      RKLKKVFEN LDVVAQPFV DSSDKNSEE KKKSENGVN 
      FSKKSRRRS KTIKAKLTK MKSAELTKR LNLNGKKLN 
      QRPSKQNLN NKVGPNGEI EFLLKPTDT FEGRNPIVT 
      SLRHLLKID VDTFEKKKI EQDVHPHWK KEILTMSQV 
      DISKYFPLD QDKLAKAVN YLKESTCQK YNLNINRSL 
      NIIIDYLKQ LVQLIVRKY REHWENDRI SGTCSCLDI 
      NEHVPWLFN QHLRNLESR EKHMLQELQ KTFDQLIKS 
      VKELAHKDV SHCLKALNQ IETSILNYE IRGIKVLSY 
      VTGMETFTL KYKTMNPKV DTTFVLNHV QNVVNNLEE 
      ARNDIEIEV TDEVDDVSF VESYKSETP IELFFRELS 
      QVGEPDPKN KRKEIEAKL NTIANGFHL INSIMALCD 
      DDSSEQKFY RIADLMYPL RGTYQKPQS YPEISEYMP 
      KRLKSQISS LQNCNSFQK LLGGEIRIA TKERRVNYI 
      ISAGVNFTA VELGLDINS QPLAVKRIP RESWVGKMV 
      KTLAEPLLG LMDNHVLHY FACEYEGNE LILATPLCK 
      WTLGQYIQL LRHSTASTV PNLTGLQLV KQFLAGLMV 
      LHKCEMPIV HGNLKPSNI FIDFNGLVK IAEFGIHKA 
      LYKIIEAPK SSLIWFAKE TLDIYKNSS IVDCTLKSD 
      IQVAGMLIF YILSFGKHP FGCDTDEIL KNIQGGAPR 
      LNIKHLEMK DLLSWMMAS DINERPNIE QVLAHPFFW 
      SHDHKWSFI LCCSGINDT GYILPLNID KLHRSIDAY 
      ASFYIKLEW HEEIAKAFP SVGITEYNN VVGLLNFIK 
      NCHDHQAER ELTVSDLSC VILSLFPAL PVALYRALQ 
      NTNWFRHPV FIPFCQLF
    CDS sequence
    • >TC034408 RA
      atgtcttcg aaagccaag aaaggaaag aaaaaaccc 
      aaatcggtt aacgatgtg agcaaaagc aaagacact 
      ctgaccaat aaaaaaatc cacgcctat ttcaccaat 
      gaggagatc gccgaaatc aagtccatc atgaagacg 
      aaggacaca ggagtcccc ttcgccatc gccgcaacc 
      ctcctctac aaacccgac ctactgaaa aaactcctc 
      aaagacggc ttcaacgtc aaccaaacc ggccagaac 
      aacatgacg gccctgatg tgggccgtc aagcacaaa 
      tatttggac atcgtcgac tatctccta acgaacgga 
      gccgaccca actatcaaa accgacagc ggcgataac 
      gtgctaatc ctagctctg gaacacaaa ctttgggac 
      gagcagtcc atgatcaat ctctggaag gccgtcaaa 
      aaggtcagt ttcatcgac gttaacttc accagcaag 
      aacggccac aacatgctc cacatgtgc gtgcgaagg 
      gagtgggag cagctcctg aaactcctc ctgaacgaa 
      aagcctgac gtagatgcg gggaactcg aacggggtt 
      acgcccctg atgctggcg tgtttccgc aacaatatc 
      agtatcatt acaacgttg atcaatgcg ggcgccgat 
      atactgaag gaggacaac cacgggagg atgactctg 
      tgttacgcc atctcctcg tgcatgaaa gtgagcaag 
      ccgccgttt ccggccacc gaaaaaatc ataaccgag 
      ttgaaaaac aaatcgtcg ttggagaat tacctgaaa 
      cggcgattg gaactgata attgcaacg gcaaagaaa 
      tcggagatc tcgtgcgca acagccgag atgctaatc 
      aagattgtt caattcgcg gtccagtat atggaagag 
      ggtattcca acgtttttg gagtgtgac gtatttgca 
      caattgtta gaaattatc acgtcgagg ttggacaat 
      agtgccatc gtcaaattt ctcctcgag gtgtgcgag 
      gagatcctt ttttacaac gaacaatgt gtcgggtac 
      aaagtcgag ttgggtaat aaactggtt gaaggtttc 
      tacgcaagc ggtctagtg gataatgtc ttggaagtt 
      ctgcgaaag cagagtgaa gaagccaaa attgcgttc 
      aaggtgcta cttttgggg tttaacaat gacgaacgg 
      tgggaaaaa accatgagg gacaacagt gacgtcctt 
      tcgccgctg ctaaacgac cctcaaaac ttggatgaa 
      gacgaaagt cgggcaaaa atcttgaag aacaagaaa 
      cgaaaattg aagaaagtc tttgagaat ttggacgtg 
      gtggcgcaa ccgttcgtc gattcatcg gacaagaat 
      tcggaagaa aagaagaag agtgagaat ggtgttaac 
      ttctcgaag aagtcaaga agacgttcc aagacaatc 
      aaagctaag ctcacaaaa atgaaaagt gcagaactt 
      acgaaaaga ctgaacttg aacgggaaa aaactcaac 
      caaagaccc tcaaaacaa aatttgaat aacaaagtc 
      gggcccaat ggagaaatt gaattttta ctcaaaccg 
      actgatacg ttcgaaggt cgcaatccg atagttacc 
      tcgcttcgg cacttgtta aaaattgac gtggacaca 
      tttgagaag aaaaagata gaacaagac gttcaccca 
      cactggaag aaggaaatc ttaacaatg tcacaggtt 
      gatatttcc aaatatttt cctttagat caggacaaa 
      cttgccaag gctgttaac tacctcaag gaatcaact 
      tgccaaaaa tacaatcta aatattaac cgttccctg 
      aacattatt atagactat ctgaaacaa ctcgtccaa 
      ctgatagtg agaaagtac cgagagcat tgggaaaat 
      gatagaata tcgggcact tgctcctgt ttagatata 
      aatgaacac gtcccttgg ttattcaac caacacttg 
      aggaaccta gaatcgcgc gagaagcac atgcttcaa 
      gagttgcaa aaaaccttc gaccaactg atcaaatcc 
      gtgaaagaa ctggcccac aaggacgtg tctcattgc 
      ctcaaagcc ctcaaccag attgagacc tcaatcctc 
      aattacgaa atccgcgga atcaaagtt ctctcctac 
      gttaccggc atggaaacc tttactctc aaatacaaa 
      acaatgaac ccgaaagtg gacaccact ttcgtcctg 
      aaccacgtg cagaacgtg gtcaataat ctcgaagag 
      gcgcgaaac gacatcgaa attgaagta accgacgaa 
      gtcgatgac gtctcgttc gtcgaatcg tacaaatca 
      gaaaccccg attgagctt tttttccgc gaactgtcc 
      caagtcggg gaacccgac ccaaaaaac aagcgcaaa 
      gaaatcgag gcgaaactg aacacaatc gctaacggg 
      tttcacttg attaactcg ataatggcg ctgtgtgac 
      gacgactca agtgagcaa aaattctac cgaattgcc 
      gacctgatg tatccgctg cggggcacg taccagaaa 
      ccgcagtcc tatccggag atttccgaa tatatgccg 
      aaacggctc aaatcgcaa atctccagc ttgcagaac 
      tgtaactcc ttccagaag ctgctagga ggggaaatt 
      cgcatcgcc accaaggag cggagagtt aattacatt 
      atttcggcc ggcgttaac ttcacggcc gtggagttg 
      gggttggac atcaacagc cagccgctg gctgtgaag 
      agaatcccg cgggagagc tgggtgggg aaaatggtc 
      aagacgctg gcggagcct ttgctaggc ttgatggac 
      aatcacgtt ttgcactat ttcgcgtgt gaatacgag 
      gggaatgag ttgattctt gccactcca ttgtgtaaa 
      tggaccctg gggcagtac attcaattg ttgagacat 
      agcactgcg tccactgtt cccaacttg acggggctc 
      cagcttgtt aagcagttt ctggccggt ctgatggtc 
      ctgcacaag tgcgaaatg cccatagtg catggcaat 
      ctcaaaccg tcaaacatt ttcatagat tttaacggc 
      ttggtgaaa attgccgag ttcggcatt cacaaagct 
      ctgtacaag attatcgaa gcaccgaaa agctcgctg 
      atttggttc gccaaagaa actttggat atttacaaa 
      aatagctca attgttgac tgtacgctc aagtcggac 
      atacaagtg gccggaatg ctcattttt tacattcta 
      tcgttcggg aagcacccg tttggttgc gataccgac 
      gagattttg aaaaatata caaggggga gcccccaga 
      cttaacata aaacacctc gagatgaag gacctgctt 
      tcgtggatg atggcgagt gacatcaac gagcggccc 
      aacattgag caggtgtta gcgcaccct tttttctgg 
      tcccacgac cacaaatgg agtttcatt ctttgttgc 
      tccggaatt aacgacact ggctacatt ttaccgctc 
      aacatcgac aaacttcac aggagcatc gatgcttac 
      gccagtttt tacattaaa ttagaatgg catgaagaa 
      atcgcaaag gcgttcccc agtgtggga atcaccgaa 
      tataacaat gttgtcgga ttgttgaat tttatcaaa 
      aactgtcac gaccaccag gcagagcgc gaattgacg 
      gtttcagat ttaagttgc gtcatttta tccctgttt 
      ccggcgctt ccggtggct ctgtacagg gcgctgcag 
      aacacaaac tggttcagg catccagtt tttatcccc 
      ttttgtcag ttattttga
    mRNA sequence
    • >TC034408 RA
      tcagaaaat tcatgtctt cgaaagcca agaaaggaa 
      agaaaaaac ccaaatcgg ttaacgatg tgagcaaaa 
      gcaaagaca ctctgacca ataaaaaaa tccacgcct 
      atttcacca atgaggaga tcgccgaaa tcaagtcca 
      tcatgaaga cgaaggaca caggagtcc ccttcgcca 
      tcgccgcaa ccctcctct acaaacccg acctactga 
      aaaaactcc tcaaagacg gcttcaacg tcaaccaaa 
      ccggccaga acaacatga cggccctga tgtgggccg 
      tcaagcaca aatatttgg acatcgtcg actatctcc 
      taacgaacg gagccgacc caactatca aaaccgaca 
      gcggcgata acgtgctaa tcctagctc tggaacaca 
      aactttggg acgagcagt ccatgatca atctctgga 
      aggccgtca aaaaggtca gtttcatcg acgttaact 
      tcaccagca agaacggcc acaacatgc tccacatgt 
      gcgtgcgaa gggagtggg agcagctcc tgaaactcc 
      tcctgaacg aaaagcctg acgtagatg cggggaact 
      cgaacgggg ttacgcccc tgatgctgg cgtgtttcc 
      gcaacaata tcagtatca ttacaacgt tgatcaatg 
      cgggcgccg atatactga aggaggaca accacggga 
      ggatgactc tgtgttacg ccatctcct cgtgcatga 
      aagtgagca agccgccgt ttccggcca ccgaaaaaa 
      tcataaccg agttgaaaa acaaatcgt cgttggaga 
      attacctga aacggcgat tggaactga taattgcaa 
      cggcaaaga aatcggaga tctcgtgcg caacagccg 
      agatgctaa tcaagattg ttcaattcg cggtccagt 
      atatggaag agggtattc caacgtttt tggagtgtg 
      acgtatttg cacaattgt tagaaatta tcacgtcga 
      ggttggaca atagtgcca tcgtcaaat ttctcctcg 
      aggtgtgcg aggagatcc ttttttaca acgaacaat 
      gtgtcgggt acaaagtcg agttgggta ataaactgg 
      ttgaaggtt tctacgcaa gcggtctag tggataatg 
      tcttggaag ttctgcgaa agcagagtg aagaagcca 
      aaattgcgt tcaaggtgc tacttttgg ggtttaaca 
      atgacgaac ggtgggaaa aaaccatga gggacaaca 
      gtgacgtcc tttcgccgc tgctaaacg accctcaaa 
      acttggatg aagacgaaa gtcgggcaa aaatcttga 
      agaacaaga aacgaaaat tgaagaaag tctttgaga 
      atttggacg tggtggcgc aaccgttcg tcgattcat 
      cggacaaga attcggaag aaaagaaga agagtgaga 
      atggtgtta acttctcga agaagtcaa gaagacgtt 
      ccaagacaa tcaaagcta agctcacaa aaatgaaaa 
      gtgcagaac ttacgaaaa gactgaact tgaacggga 
      aaaaactca accaaagac cctcaaaac aaaatttga 
      ataacaaag tcgggccca atggagaaa ttgaatttt 
      tactcaaac cgactgata cgttcgaag gtcgcaatc 
      cgatagtta cctcgcttc ggcacttgt taaaaattg 
      acgtggaca catttgaga agaaaaaga tagaacaag 
      acgttcacc cacactgga agaaggaaa tcttaacaa 
      tgtcacagg ttgatattt ccaaatatt ttcctttag 
      atcaggaca aacttgcca aggctgtta actacctca 
      aggaatcaa cttgccaaa aatacaatc taaatatta 
      accgttccc tgaacatta ttatagact atctgaaac 
      aactcgtcc aactgatag tgagaaagt accgagagc 
      attgggaaa atgatagaa tatcgggca cttgctcct 
      gtttagata taaatgaac acgtccctt ggttattca 
      accaacact tgaggaacc tagaatcgc gcgagaagc 
      acatgcttc aagagttgc aaaaaacct tcgaccaac 
      tgatcaaat ccgtgaaag aactggccc acaaggacg 
      tgtctcatt gcctcaaag ccctcaacc agattgaga 
      cctcaatcc tcaattacg aaatccgcg gaatcaaag 
      ttctctcct acgttaccg gcatggaaa cctttactc 
      tcaaataca aaacaatga acccgaaag tggacacca 
      ctttcgtcc tgaaccacg tgcagaacg tggtcaata 
      atctcgaag aggcgcgaa acgacatcg aaattgaag 
      taaccgacg aagtcgatg acgtctcgt tcgtcgaat 
      cgtacaaat cagaaaccc cgattgagc tttttttcc 
      gcgaactgt cccaagtcg gggaacccg acccaaaaa 
      acaagcgca aagaaatcg aggcgaaac tgaacacaa 
      tcgctaacg ggtttcact tgattaact cgataatgg 
      cgctgtgtg acgacgact caagtgagc aaaaattct 
      accgaattg ccgacctga tgtatccgc tgcggggca 
      cgtaccaga aaccgcagt cctatccgg agatttccg 
      aatatatgc cgaaacggc tcaaatcgc aaatctcca 
      gcttgcaga actgtaact ccttccaga agctgctag 
      gaggggaaa ttcgcatcg ccaccaagg agcggagag 
      ttaattaca ttatttcgg ccggcgtta acttcacgg 
      ccgtggagt tggggttgg acatcaaca gccagccgc 
      tggctgtga agagaatcc cgcgggaga gctgggtgg 
      ggaaaatgg tcaagacgc tggcggagc ctttgctag 
      gcttgatgg acaatcacg ttttgcact atttcgcgt 
      gtgaatacg aggggaatg agttgattc ttgccactc 
      cattgtgta aatggaccc tggggcagt acattcaat 
      tgttgagac atagcactg cgtccactg ttcccaact 
      tgacggggc tccagcttg ttaagcagt ttctggccg 
      gtctgatgg tcctgcaca agtgcgaaa tgcccatag 
      tgcatggca atctcaaac cgtcaaaca ttttcatag 
      attttaacg gcttggtga aaattgccg agttcggca 
      ttcacaaag ctctgtaca agattatcg aagcaccga 
      aaagctcgc tgatttggt tcgccaaag aaactttgg 
      atatttaca aaaatagct caattgttg actgtacgc 
      tcaagtcgg acatacaag tggccggaa tgctcattt 
      tttacattc tatcgttcg ggaagcacc cgtttggtt 
      gcgataccg acgagattt tgaaaaata tacaagggg 
      gagccccca gacttaaca taaaacacc tcgagatga 
      aggacctgc tttcgtgga tgatggcga gtgacatca 
      acgagcggc ccaacattg agcaggtgt tagcgcacc 
      cttttttct ggtcccacg accacaaat ggagtttca 
      ttctttgtt gctccggaa ttaacgaca ctggctaca 
      ttttaccgc tcaacatcg acaaacttc acaggagca 
      tcgatgctt acgccagtt tttacatta aattagaat 
      ggcatgaag aaatcgcaa aggcgttcc ccagtgtgg 
      gaatcaccg aatataaca atgttgtcg gattgttga 
      attttatca aaaactgtc acgaccacc aggcagagc 
      gcgaattga cggtttcag atttaagtt gcgtcattt 
      tatccctgt ttccggcgc ttccggtgg ctctgtaca 
      gggcgctgc agaacacaa actggttca ggcatccag 
      tttttatcc ccttttgtc agttatttt gaccgattt 
      tatttatac ttttcattg tgatatttt tcgaataaa 
      aacctcgtt tgtgt
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