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Gene information
General information
TC012612 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12150884..12154037
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012612
    iBeetle screen iB_09489

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012612 RA
      MSSLPKPIG SGASQKFFH VTKDPPLFF IMMRLNVKV 
      ALLFLTTIG FFTVLILWS VCGKEPNNL QDWRRTGKF 
      SLDRLAELQ SAEEIDCDI NGEYVIGCR KEGDEVYLP 
      FSFLHKYFE VYGKLATYD GLERFEWSH SYSRVYHPK 
      GKYDPRGVF MYFENYNVE VRDRVKCVS AVEGVPIST 
      QWESQGYYY PTQIAQFGL SHYSKNLTE PEPRRKIIE 
      DGERELAKW GVPHPGTIE RVYDKTAGS KVLKFKTGE 
      LYLDAIKLK MEHVLDFIM SLNIVLSGN SSFSIVLQN 
      RETREIYNL HYITSDVLI TVQDNNVYH GLGMNQEWK 
      KLTRDLIVD LQKGLNYLD KDKSKRKIP KSKLKIITI 
      LLRGYGAID NLTLSSSEH IQQFYDAAE WFVRHQDTE 
      TGGWPIPVK RKLASGFHD LQSGWYSAM GQGHAISVL 
      SRAYHHSGG DVRYLQAAL AGLKPFQVP STKGGVLAT 
      FLNKYHWYE EYPTKPASF VLNGFIYSL LGLYDLMTI 
      APPGQADEA EFLFREGMI SLKGMLLFY DMGSVTSYD 
      LRHVTLGVA PNLARWDYH ATHINQLLL LSTIENEPL 
      FAQTAERWI GYMAGKRAA HN
    CDS sequence
    • >TC012612 RA
      atgagcagc ctaccgaaa ccaattggc tccggggca 
      agccaaaaa tttttccac gttaccaag gaccccccc 
      ttgtttttt attatgatg cgtctgaac gtaaaagtc 
      gctttgttg ttcctgaca acgatcggt tttttcacc 
      gttttgatc ttatggtcg gtttgcggc aaagagccg 
      aacaatttg caagactgg cggaggact ggtaaattc 
      agtctggac cggttggcc gaactgcag agtgccgaa 
      gaaatagat tgtgatata aacggcgaa tatgttata 
      ggatgtagg aaagaagga gacgaagtg tacctcccc 
      ttctcgttt ttgcataag tatttcgaa gtctacgga 
      aagctggcc acttacgac ggtttggaa aggtttgag 
      tggtcgcac agttatagc agggtttac cacccgaaa 
      gggaagtat gacccgcga ggggtcttc atgtacttt 
      gaaaattac aacgtcgag gtgcgagac cgcgtcaag 
      tgcgtaagc gcagtcgag ggagtccct atctcgacc 
      caatgggaa agtcagggt tactattac ccaactcaa 
      atcgcccag ttcgggcta tctcactac agcaaaaat 
      ctcacagag ccggaacca aggcggaag ataatcgaa 
      gacggcgag agggaactg gccaaatgg ggggttccc 
      catcccggc accatcgaa cgggtgtac gacaaaacc 
      gccggcagc aaggtgtta aagttcaaa accggcgaa 
      ctttattta gacgcgatc aagctgaaa atggagcac 
      gtacttgat tttataatg agtctcaat atcgtccta 
      agtggcaat agtagtttt agcatcgtt ctacaaaac 
      cgggaaacc cgcgagatt tacaatctt cactatatc 
      acctccgat gtactcatc acggtgcag gacaataac 
      gtgtaccat ggtctcgga atgaaccaa gaatggaag 
      aaactcacc cgcgatctc atcgtcgac ttacaaaag 
      ggtctaaac tacctcgac aaagacaaa tccaagcgc 
      aaaatacca aagtcaaaa ttgaaaata attacaatt 
      ttgttacgc ggttacggc gccatcgac aatctcaca 
      ctatcgtca tcagaacat atccaacaa ttctacgac 
      gccgccgaa tggtttgtg aggcaccag gacaccgaa 
      accggggga tggccgatt ccagtcaaa agaaaacta 
      gcctcgggt ttccacgac ttgcaatca ggttggtac 
      tcggcgatg ggtcagggc cacgcgatt tccgtcttg 
      tcccgggcg taccaccac tctggtggc gacgttcgg 
      tacttgcag gcggctctc gccggcctt aagcccttc 
      caagtgccg agcaccaag ggaggcgtc ctggcgacg 
      ttcctcaac aagtaccat tggtacgag gagtaccca 
      acgaaacca gcgtcattt gtcttgaac ggctttatt 
      tactccctt ctcggatta tacgacttg atgaccatt 
      gccccgcca gggcaggcg gacgaggcc gagtttctt 
      ttccgagaa gggatgatt tccctaaag ggaatgctg 
      ctcttttac gatatgggc agtgttact tcgtacgat 
      ttgagacat gtgacgcta ggtgtggcc ccgaatttg 
      gctcggtgg gattatcat gccacacat atcaatcag 
      ttgttattg ctgagtact attgagaat gagccgttg 
      tttgctcaa accgccgaa agatggatc ggttacatg 
      gcgggaaaa cgagcagct cataattga
    mRNA sequence
    • >TC012612 RA
      ttgcaatgt tgacagttc tcaataaaa acatctaaa 
      gcgaatcgg aattcggag aatcgatta cttaaaaat 
      gtacatcga cagataagc taaatcaat gcaaacaga 
      agttattaa acatgtgag tgactaatg agcagccta 
      ccgaaacca attggctcc ggggcaagc caaaaattt 
      ttccacgtt accaaggac ccccccttg ttttttatt 
      atgatgcgt ctgaacgta aaagtcgct ttgttgttc 
      ctgacaacg atcggtttt ttcaccgtt ttgatctta 
      tggtcggtt tgcggcaaa gagccgaac aatttgcaa 
      gactggcgg aggactggt aaattcagt ctggaccgg 
      ttggccgaa ctgcagagt gccgaagaa atagattgt 
      gatataaac ggcgaatat gttatagga tgtaggaaa 
      gaaggagac gaagtgtac ctccccttc tcgtttttg 
      cataagtat ttcgaagtc tacggaaag ctggccact 
      tacgacggt ttggaaagg tttgagtgg tcgcacagt 
      tatagcagg gtttaccac ccgaaaggg aagtatgac 
      ccgcgaggg gtcttcatg tactttgaa aattacaac 
      gtcgaggtg cgagaccgc gtcaagtgc gtaagcgca 
      gtcgaggga gtccctatc tcgacccaa tgggaaagt 
      cagggttac tattaccca actcaaatc gcccagttc 
      gggctatct cactacagc aaaaatctc acagagccg 
      gaaccaagg cggaagata atcgaagac ggcgagagg 
      gaactggcc aaatggggg gttccccat cccggcacc 
      atcgaacgg gtgtacgac aaaaccgcc ggcagcaag 
      gtgttaaag ttcaaaacc ggcgaactt tatttagac 
      gcgatcaag ctgaaaatg gagcacgta cttgatttt 
      ataatgagt ctcaatatc gtcctaagt ggcaatagt 
      agttttagc atcgttcta caaaaccgg gaaacccgc 
      gagatttac aatcttcac tatatcacc tccgatgta 
      ctcatcacg gtgcaggac aataacgtg taccatggt 
      ctcggaatg aaccaagaa tggaagaaa ctcacccgc 
      gatctcatc gtcgactta caaaagggt ctaaactac 
      ctcgacaaa gacaaatcc aagcgcaaa ataccaaag 
      tcaaaattg aaaataatt acaattttg ttacgcggt 
      tacggcgcc atcgacaat ctcacacta tcgtcatca 
      gaacatatc caacaattc tacgacgcc gccgaatgg 
      tttgtgagg caccaggac accgaaacc gggggatgg 
      ccgattcca gtcaaaaga aaactagcc tcgggtttc 
      cacgacttg caatcaggt tggtactcg gcgatgggt 
      cagggccac gcgatttcc gtcttgtcc cgggcgtac 
      caccactct ggtggcgac gttcggtac ttgcaggcg 
      gctctcgcc ggccttaag cccttccaa gtgccgagc 
      accaaggga ggcgtcctg gcgacgttc ctcaacaag 
      taccattgg tacgaggag tacccaacg aaaccagcg 
      tcatttgtc ttgaacggc tttatttac tcccttctc 
      ggattatac gacttgatg accattgcc ccgccaggg 
      caggcggac gaggccgag tttcttttc cgagaaggg 
      atgatttcc ctaaaggga atgctgctc ttttacgat 
      atgggcagt gttacttcg tacgatttg agacatgtg 
      acgctaggt gtggccccg aatttggct cggtgggat 
      tatcatgcc acacatatc aatcagttg ttattgctg 
      agtactatt gagaatgag ccgttgttt gctcaaacc 
      gccgaaaga tggatcggt tacatggcg ggaaaacga 
      gcagctcat aattgaatt gtttatttc gatggttgt 
      gcgaaactt gcagcgtat gtcgggtat tttgttcag 
      tattttttt gactcttct ttatttttg tgccatact 
      caaagtatt tgatcggaa ctattcttt gttgttttt 
      gatctgcca ttaaatggt agttttgtt tgttgttcg 
      aatggacca aattagctt cttcagagt ttacgttcg 
      ttggttagt gttaagttt ttcggtact ttgttaggc 
      ttcaagttt ggaatttta tacgttttt tacctagac 
      ttccgacca gtgttctaa atctatgcg agttcaaca 
      aattcaatt gaaatctca ttcttagga atatcggct 
      gacaatctt agaacgtaa aggattctg tcgcataga 
      tttttcgac gtggtagat cgagtattc ttcaaagac 
      taaataggg gaaacttag gagagtcaa ttatagaaa 
      gtagtgtaa aaaagttgt acattaaaa atgggactt 
      atgtaaaaa cttgtgtac ataatatca tattgtata 
      atgttgctg caatcataa cgttaatgt taggggaga 
      aacgtagag atttataag tcttttaca gtgtataac 
      gcaaaactt aatctattg gaaaagagc ttactagtt 
      tacctctac cagctatgt agtagtctg tccaagacg 
      cagtcaagt ccgtcctgg acagccaaa aacttccaa 
      aaatgtcct gcctaaaac cacgatcaa ctatacaat 
      gtgttcata atcagcact aatcgttaa tttccgtgg 
      ccgcgattt acgtataat actcttcaa caagtactt 
      tgaattgcc gtccctaat ggcttactt ctggttttt 
      ttcactaaa agcctaatg attctaata cgttaagta 
      tgcgatgtg atattattt acgaactaa ctgtactta 
      atttcgtat cgttaggtt tgtttaaat cggggcact 
      gccaaataa tctcgcaga gaggtgtca ccaatctgc 
      actctatat caatgtttg aactgttgg atccaaaat 
      taagtgact gggacaata acacttagg gaaatatat 
      taaccatgg cctc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY