Status
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Gene information
General information
TC011896 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12139188..12141612
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011896
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09488

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011896 RA
      MGRKARFNT EGVLVKRGP GKKAKKQKD PTFPKALLN 
      GDSDAKKLS HRQKQRLKK RLVKRKDVA KVAKESSES 
      EQEDEEVWL KPSVSQNDT KVLPGEGFT DDNKSWLKP 
      KRQVVIESE ESDEEDCKV GTLNDVSDD SEPDDDDLL 
      PIEKENKIL KQQQDEEEQ LAEDELQMN IADSEKFKL 
      PSKKEREET ASLQEVQQR IRDVIGVLS DFTKLRDEK 
      HSRSDYLEM LKADLCTYY SYNDFLMEK FMQLFPLTE 
      VLEFLEASE VQRPLTIRT NSLKTRRRD LAQALINRG 
      VNLDPVGKW SKVGLVVYS SQVPIGATP EYLAGHYII 
      QGASSLLPV MALAPQENE RILDMAAAP GGKASHIAA 
      LMKNTGVLF ANDVNSERI KAIVGNFHR LGVVNSVIT 
      CVDGRKFPK ILKGFDRVL LDAPCTGTG VVAKDQSVK 
      TSKDEQDIQ RCYNLQREL LLAAIDCLN AKSETGGYL 
      VYSTCSVLP EENEWVVDY ALKKRNVKL VDTGLEFGN 
      EGFTNYRQF RFHPSLKLT RRFYPHTHN MDGFFVAKF 
      RKFSNLIPT TAGEEQGEK RELEEEVEV EEVSTKKQK 
      KNGETQPKK AKKSKENNT SQQELKEKS KEINTSKQE 
      QDEEVKTKK QKAKKVKQN GDIKDNKQE KNAVVDDGK 
      KKKNVVQNS KADTRKEGT NSPKDNKLK KEKSASPGK 
      KNVKTGEKL DWDKIPKEA SVKLNKKIK KVKKKQVGV 
      MKNKMFKKK HT
    CDS sequence
    • >TC011896 RA
      atgggcaga aaagcgcgg tttaacacg gaaggagtc 
      ctcgttaaa cgagggcct ggcaaaaag gcaaaaaaa 
      caaaaggac ccgacgttt ccgaaagcg ttactcaat 
      ggtgattcc gacgcgaaa aaactgagc cacagacag 
      aaacaacgt ttgaagaaa agactagtt aagaggaaa 
      gatgtggca aaagtcgct aaagaaagt agtgagtca 
      gagcaggag gatgaagaa gtgtggttg aagccttct 
      gtgagtcaa aatgacacg aaggttttg cctggcgag 
      ggtttcacg gacgataac aagtcctgg ctgaagccc 
      aagcggcaa gttgtgatt gaatctgag gaaagtgac 
      gaggaggat tgtaaagta gggactttg aacgacgtt 
      tcggacgat tctgaacct gacgatgac gaccttctc 
      ccgattgaa aaggagaac aagatactt aagcagcaa 
      caagatgag gaggagcag ctcgctgag gacgagctc 
      caaatgaac attgcagac agcgaaaag ttcaaactc 
      ccgtcaaag aaagaacgc gaagagacc gcaagtttg 
      caggaagtg caacaacgc atccgtgac gtgatcgga 
      gtcctctcc gacttcacc aaactccgc gatgagaaa 
      cactcacgt tccgactac ttggagatg ctaaaagcc 
      gatctttgc acctactac agctacaac gacttccta 
      atggagaag ttcatgcag ctgttcccc ctgaccgaa 
      gttttggag tttcttgaa gccagcgaa gtccaaaga 
      ccgttgacc atccgcacc aacagtctg aagacgcgg 
      cgtcgcgac ctggcccag gccctgatt aaccgcggg 
      gttaacttg gacccggtg ggaaaatgg agcaaagtg 
      ggcctcgtt gtgtattct tcccaagtt ccgatcggg 
      gcgacccct gagtatttg gccggtcat tacattatt 
      caaggcgct tcgagtttg ctaccggtc atggcgcta 
      gcgccgcaa gaaaacgag aggattctg gacatggcg 
      gcggccccc gggggcaaa gcctcgcac atcgccgcg 
      ctgatgaaa aacacgggg gtcttgttc gctaatgat 
      gtcaatagc gagcgaatc aaggcaatc gtcgggaat 
      ttccaccgg ttgggggtc gttaattcg gtcattacg 
      tgcgtggac ggccgaaaa ttcccgaaa attttgaag 
      ggatttgac agggtcctg ctggatgcg ccctgcacc 
      gggacgggg gttgtggcg aaggaccag agtgtcaag 
      actagcaag gacgagcag gacattcag cggtgctat 
      aatttgcaa agggagttg ctcttggcg gcgattgat 
      tgccttaat gccaagtcg gaaaccggc ggatatttg 
      gtttattcg acttgttcg gtgttgccg gaagagaac 
      gaatgggtg gttgattac gctctgaag aagaggaac 
      gttaagttg gtcgatacg ggactggaa ttcgggaat 
      gaaggcttt acgaattat agacagttt aggtttcac 
      ccgagtttg aaactgacg aggaggttt tacccgcat 
      acgcataac atggacggg ttttttgtg gccaagttt 
      aggaaattc tcaaatttg attccgacg actgcgggc 
      gaggagcag ggcgaaaag agggaattg gaggaggaa 
      gtggaggtt gaggaagtt agcactaaa aaacagaaa 
      aagaatgga gaaacgcag ccgaaaaag gcgaagaaa 
      agtaaggaa aataatacg agtcaacaa gaactgaag 
      gagaaaagt aaagaaatt aatacgagt aaacaagaa 
      caggacgag gaagtgaag actaaaaaa cagaaagcg 
      aaaaaggtt aagcaaaat ggggatatt aaggacaat 
      aaacaagaa aaaaatgcg gttgtagat gatggtaag 
      aagaagaaa aatgtagtg caaaatagt aaagcggat 
      acgaggaaa gaaggaacg aattcgccg aaagataac 
      aaattgaaa aaggaaaag tccgcaagt ccgggcaag 
      aagaatgtg aagactggt gaaaaactc gattgggac 
      aaaattccg aaagaagct agtgtgaag cttaataaa 
      aagattaag aaagttaag aagaaacaa gttggtgtt 
      atgaagaat aaaatgttc aaaaagaag catacataa
    mRNA sequence
    • >TC011896 RA
      ggacggttt gaaagctgt gccaacgta aatcacaag 
      ttggtcaca ttgaacgtc aagatccct taaccttaa 
      acttgtgtt ttcacgttt caaatgcag cacgtgtgt 
      tttatgaat cggtaaaat gggcagaaa agcgcggtt 
      taacacgga aggagtcct cgttaaacg agggcctgg 
      caaaaaggc aaaaaaaca aaaggaccc gacgtttcc 
      gaaagcgtt actcaatgg tgattccga cgcgaaaaa 
      actgagcca cagacagaa acaacgttt gaagaaaag 
      actagttaa gaggaaaga tgtggcaaa agtcgctaa 
      agaaagtag tgagtcaga gcaggagga tgaagaagt 
      gtggttgaa gccttctgt gagtcaaaa tgacacgaa 
      ggttttgcc tggcgaggg tttcacgga cgataacaa 
      gtcctggct gaagcccaa gcggcaagt tgtgattga 
      atctgagga aagtgacga ggaggattg taaagtagg 
      gactttgaa cgacgtttc ggacgattc tgaacctga 
      cgatgacga ccttctccc gattgaaaa ggagaacaa 
      gatacttaa gcagcaaca agatgagga ggagcagct 
      cgctgagga cgagctcca aatgaacat tgcagacag 
      cgaaaagtt caaactccc gtcaaagaa agaacgcga 
      agagaccgc aagtttgca ggaagtgca acaacgcat 
      ccgtgacgt gatcggagt cctctccga cttcaccaa 
      actccgcga tgagaaaca ctcacgttc cgactactt 
      ggagatgct aaaagccga tctttgcac ctactacag 
      ctacaacga cttcctaat ggagaagtt catgcagct 
      gttccccct gaccgaagt tttggagtt tcttgaagc 
      cagcgaagt ccaaagacc gttgaccat ccgcaccaa 
      cagtctgaa gacgcggcg tcgcgacct ggcccaggc 
      cctgattaa ccgcggggt taacttgga cccggtggg 
      aaaatggag caaagtggg cctcgttgt gtattcttc 
      ccaagttcc gatcggggc gacccctga gtatttggc 
      cggtcatta cattattca aggcgcttc gagtttgct 
      accggtcat ggcgctagc gccgcaaga aaacgagag 
      gattctgga catggcggc ggcccccgg gggcaaagc 
      ctcgcacat cgccgcgct gatgaaaaa cacgggggt 
      cttgttcgc taatgatgt caatagcga gcgaatcaa 
      ggcaatcgt cgggaattt ccaccggtt gggggtcgt 
      taattcggt cattacgtg cgtggacgg ccgaaaatt 
      cccgaaaat tttgaaggg atttgacag ggtcctgct 
      ggatgcgcc ctgcaccgg gacgggggt tgtggcgaa 
      ggaccagag tgtcaagac tagcaagga cgagcagga 
      cattcagcg gtgctataa tttgcaaag ggagttgct 
      cttggcggc gattgattg ccttaatgc caagtcgga 
      aaccggcgg atatttggt ttattcgac ttgttcggt 
      gttgccgga agagaacga atgggtggt tgattacgc 
      tctgaagaa gaggaacgt taagttggt cgatacggg 
      actggaatt cgggaatga aggctttac gaattatag 
      acagtttag gtttcaccc gagtttgaa actgacgag 
      gaggtttta cccgcatac gcataacat ggacgggtt 
      ttttgtggc caagtttag gaaattctc aaatttgat 
      tccgacgac tgcgggcga ggagcaggg cgaaaagag 
      ggaattgga ggaggaagt ggaggttga ggaagttag 
      cactaaaaa acagaaaaa gaatggaga aacgcagcc 
      gaaaaaggc gaagaaaag taaggaaaa taatacgag 
      tcaacaaga actgaagga gaaaagtaa agaaattaa 
      tacgagtaa acaagaaca ggacgagga agtgaagac 
      taaaaaaca gaaagcgaa aaaggttaa gcaaaatgg 
      ggatattaa ggacaataa acaagaaaa aaatgcggt 
      tgtagatga tggtaagaa gaagaaaaa tgtagtgca 
      aaatagtaa agcggatac gaggaaaga aggaacgaa 
      ttcgccgaa agataacaa attgaaaaa ggaaaagtc 
      cgcaagtcc gggcaagaa gaatgtgaa gactggtga 
      aaaactcga ttgggacaa aattccgaa agaagctag 
      tgtgaagct taataaaaa gattaagaa agttaagaa 
      gaaacaagt tggtgttat gaagaataa aatgttcaa 
      aaagaagca tacataaaa tcaatcatt tttatttaa 
      atgttcatt ttttattaa taaaaataa agcaattat 
      gattaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY