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Gene information
General information
TC012581 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11664694..11666737
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012581
    iBeetle screen iB_09486

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012581 RA
      MWSKKNNEK DEKTVGRTT QTQVTRTSQ TVTIRTIAQ 
      HSQTISVRN NNGSIQSST SLLPLNLNS SSHLPLPNA 
      RARSSSTSY AVDAKPINV NYRSPYQAY SSNNYLPST 
      LGASNFNRN SPVYSSYQP PRPQTALPP VLPGTVQPK 
      PETASKDKK LVVKKTGKF YDGACAIPT YATHSKHRG 
      EVLIINNIM FKEKGYRHG AKADHENLK SVFKQMGFK 
      VTFERDLRA SSMASAIKS FSQKSSLKT ADIAVVVIM 
      SHGTRSETI MDTEVFGLD DKSLLVEDI LSHFNEDNC 
      KSMKGKPKI FIFQCCRGD NEQFLQSDA IRTTQKASV 
      LADALIAYS TLPGFVSHR DPQSGTWYI STLCEVFCQ 
      HAHEYHVED LLKMVDVKL TTFASGRVR QTTHYESRG 
      FKRCYLHPV
    CDS sequence
    • >TC012581 RA
      atgtggtcg aaaaagaac aatgaaaaa gacgaaaaa 
      acggttggg aggacaacc caaactcaa gttactcgt 
      actagtcaa actgtgaca attaggaca attgcgcaa 
      cactctcag accatttct gttcgtaac aacaatggt 
      agtattcag tcaagtact agtttgtta cccctgaat 
      ttaaacagc tccagccac ttgcctctt ccaaacgct 
      cgagcgagg tcgtcatca acttcttac gctgttgat 
      gctaaacct ataaatgta aattaccga agtccttat 
      caggcctac agtagcaac aattacctt ccatcaaca 
      ctcggagcc agtaacttc aaccgaaat tctcccgtt 
      tatagcagt tatcaaccc ccaaggccc caaacggcc 
      ttgcccccg gttttgcca ggcactgtc caacccaaa 
      ccggaaacc gcctccaaa gataaaaag ttggtggta 
      aaaaagaca gggaaattc tacgacggt gcgtgtgca 
      atcccaacc tacgcaacg cactcaaaa caccggggc 
      gaagtcctg attatcaac aacataatg ttcaaggag 
      aaaggctac cggcacggg gcaaaagcc gaccacgaa 
      aatctcaaa agcgttttc aaacaaatg gggttcaaa 
      gtcaccttc gagcgcgac ttgcgagcc tcctccatg 
      gcgtcggcc atcaagagc ttcagccag aaatcctcg 
      ctgaagacc gcagacatt gcagtcgtg gtcatcatg 
      agccacggc acccggagc gagaccatc atggacacg 
      gaggtgttc ggcttggac gataagtcc ctccttgtg 
      gaagatatc ctaagccac ttcaatgaa gacaactgc 
      aaaagcatg aaaggcaaa ccaaaaata ttcattttc 
      cagtgctgt cggggcgac aacgagcag tttctgcaa 
      agcgacgcc attcgcaca acgcaaaaa gcgtcagtt 
      cttgccgac gcacttatc gcatattca actctgcca 
      gggtttgtg tcgcatcgg gacccccag tcgggcacg 
      tggtacatc agcactttg tgcgaggtg ttctgccaa 
      catgcacac gagtaccat gtggaggat ttgttgaaa 
      atggtcgat gtcaagttg acgactttt gcaagcggg 
      cgggtgaga cagaccacg cactatgag agcaggggg 
      ttcaagcgg tgctacttg catccagtg taa
    mRNA sequence
    • >TC012581 RA
      ctcaaacag cgcactgag caggcttaa gtcagaatt 
      tagaacaga gtttgattt tatcagtgt tcgtgtaaa 
      tactaacct ttttgtgta aatctatta agaataaca 
      attttttaa tacgaattt gttggtatt tatgtataa 
      tttattgtt atcttatct aaaaataag ctatcgtca 
      caaagaagg aaaactgta cagatacga aaatgtggt 
      cgaaaaaga acaatgaaa aagacgaaa aaacggttg 
      ggaggacaa cccaaactc aagttactc gtactagtc 
      aaactgtga caattagga caattgcgc aacactctc 
      agaccattt ctgttcgta acaacaatg gtagtattc 
      agtcaagta ctagtttgt tacccctga atttaaaca 
      gctccagcc acttgcctc ttccaaacg ctcgagcga 
      ggtcgtcat caacttctt acgctgttg atgctaaac 
      ctataaatg taaattacc gaagtcctt atcaggcct 
      acagtagca acaattacc ttccatcaa cactcggag 
      ccagtaact tcaaccgaa attctcccg tttatagca 
      gttatcaac ccccaaggc cccaaacgg ccttgcccc 
      cggttttgc caggcactg tccaaccca aaccggaaa 
      ccgcctcca aagataaaa agttggtgg taaaaaaga 
      cagggaaat tctacgacg gtgcgtgtg caatcccaa 
      cctacgcaa cgcactcaa aacaccggg gcgaagtcc 
      tgattatca acaacataa tgttcaagg agaaaggct 
      accggcacg gggcaaaag ccgaccacg aaaatctca 
      aaagcgttt tcaaacaaa tggggttca aagtcacct 
      tcgagcgcg acttgcgag cctcctcca tggcgtcgg 
      ccatcaaga gcttcagcc agaaatcct cgctgaaga 
      ccgcagaca ttgcagtcg tggtcatca tgagccacg 
      gcacccgga gcgagacca tcatggaca cggaggtgt 
      tcggcttgg acgataagt ccctccttg tggaagata 
      tcctaagcc acttcaatg aagacaact gcaaaagca 
      tgaaaggca aaccaaaaa tattcattt tccagtgct 
      gtcggggcg acaacgagc agtttctgc aaagcgacg 
      ccattcgca caacgcaaa aagcgtcag ttcttgccg 
      acgcactta tcgcatatt caactctgc cagggtttg 
      tgtcgcatc gggaccccc agtcgggca cgtggtaca 
      tcagcactt tgtgcgagg tgttctgcc aacatgcac 
      acgagtacc atgtggagg atttgttga aaatggtcg 
      atgtcaagt tgacgactt ttgcaagcg ggcgggtga 
      gacagacca cgcactatg agagcaggg ggttcaagc 
      ggtgctact tgcatccag tgtaaagtt ttctagtat 
      tttattcaa aaagcgcat ttaggagtt gggttgatg 
      caaatgctg tagcgctat gattaacgt attggtatt 
      cgcgacata ttttaacta aaaatccta cggcggtat 
      tatcgtttt tgaataaaa agatctcat gtatcaacc 
      catctccct aatattttt ttaaatttt atttcgtta 
      gtattaagt acctggaaa tacagagtt cttggttaa 
      tgaagaagt ttacttcag ctggttgct tccacaaaa 
      aaaatctga gagcttagt tggtcaact gacatcaac 
      tttttctag ctctagtat gaccgtaac agtgttgaa 
      ttgattcct aagcttaaa aatctccgt atgtgacgc 
      catctcttc agtaataca cgaaccaat cagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY