Status
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Gene information
General information
TC011919 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11647259..11651286
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011919
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09485

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4

    previtellogenic egg chamber potentiallynot present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011919 RA
      MGFKDRTYL CFALLILFS VFVISAGQN FWKTTQFNT 
      EEPEDKRIK IVWCTISRA EQRKCKNFA MANERDQIR 
      VGYDYFKIE CLQAFDKDE CMTLLDQEK ATLTTLDAG 
      EVFVGGRYN SLVPIAQEV LEGGLQHYY AVAAVKKGT 
      LPHVNSLFD LRGVKACFA GVETFAGWV VPIYTLMNE 
      GGLDVVDCN NHVKSATKY FGPSCAVNC LTNKYNPIG 
      DNSDRLCQL CIGKIPGGR CTSSDPYSG YEGAFRCLL 
      EAGDVAFVK HTTIPSLVA GDNFVSISK DSFELLCKD 
      GSRRPIDDY MSCNWGKVP SDAIVTTSA SSFEKRKIY 
      QKFLEKAAK MYSFGNQNN GTHRYDDQN RNSYDDRQP 
      QYDQFGNRI DQPNYDQYG GRPRYKRQN FGDFGTQNR 
      PQHDPNIPQ YEENRPQYD RNNYNPYGR DRPHNESDY 
      RPYGQDKRP YDQEPGSRD RGQEYPFDP RYFNREEYG 
      ENIDPYGTR RKPSVYNNS IPDYYDYNR NNTFYEVFN 
      LFESSPRYG IHGNLLFQD MTREIAPLN ENLQTYSNF 
      LGSSMNIIM GVRVCQVNR MTLCVTSDV ERNKCVKMR 
      TALKAHLLK PEMDCYKGH SQIHCMQAI RSGLADVAV 
      LDASDVYTA GLNFDLIPF MSEMYNLNE PEYYVVAVG 
      KESDPSTEL TYLRGKYTC HGGINTAAG WVYPLAFLI 
      SNGWIRSYG CNSIRAAAE YFSKSCVPG ALSTEYNTG 
      VPYDNMCHL CHGASFRYC RRDASEDYY GHTGAFRCL 
      VEGGGQVAF VTHTTVTQN TNGKREEWW ARDNLNDDF 
      ELLCPDGTR AEINEYEKC NLGKVKANA IVTRGGYGY 
      NDTHVNAFI NLFLYAQNF YGRKTADEF SFSMFSSTP 
      PYTDLIFQD ATTQLKVIP PEKRHYAEY LGSDFLRAR 
      RIVDCHAGS ASLSAPITL LTTIVAFVI VS
    CDS sequence
    • >TC011919 RA
      atgggcttt aaagatagg acttatctt tgctttgct 
      ctgctgata ttgtttagt gtttttgtg ataagtgca 
      ggccagaat ttttggaaa accacgcaa tttaacacc 
      gaggagccc gaagataaa cgcattaag atagtatgg 
      tgcacaata agccgggca gagcaacgg aaatgcaaa 
      aacttcgct atggcaaac gaacgcgac cagatccga 
      gtgggctac gattatttc aaaatcgag tgtttacaa 
      gcgtttgat aaagacgag tgtatgact ctcctagac 
      caggagaag gccactctg accaccctt gacgcgggg 
      gaggtgttc gtgggcggg cgctacaac agtctcgtg 
      cccattgcc caggaggtc ctagaaggg gggctccag 
      cactattac gccgtcgcg gcggtcaaa aagggcacc 
      ctgccccat gtcaattcg ttgtttgat ttgagaggg 
      gtcaaggcc tgcttcgca ggcgttgaa acttttgct 
      ggatgggtt gtcccgatt tacacattg atgaacgag 
      ggtgggtta gatgttgtt gattgcaat aatcacgtc 
      aagtcggct acgaaatac tttgggccg tcgtgcgcc 
      gtcaactgt ctcaccaac aagtacaac cccattggt 
      gataattcc gacaggttg tgccaactt tgtatcgga 
      aaaatcccc ggggggcgg tgcactagc tctgacccc 
      tactcaggt tacgagggg gcgttccgt tgtctcttg 
      gaggccgga gatgtagca tttgtcaaa catacaacg 
      attccgtca cttgtggcc ggagacaat tttgtgtcg 
      atttcgaaa gattccttc gaattgcta tgcaaggac 
      ggcagtcgg cgcccgatt gacgactac atgtcgtgc 
      aattggggc aaagtcccg tcggacgca atcgtgacc 
      acttcagcc tcttccttt gaaaaacgc aagatttac 
      caaaaattc ctcgaaaag gccgcgaaa atgtacagt 
      tttggcaat cagaacaac gggacgcat cgctacgat 
      gatcagaat cggaattct tacgatgat cggcagcca 
      cagtatgat cagttcggg aacaggatt gatcagcct 
      aactatgat cagtacgga gggaggccg aggtacaaa 
      aggcagaat tttggggat tttgggacg caaaatagg 
      ccccaacat gaccccaac atcccacag tatgaggaa 
      aaccggccg cagtatgac cgaaataat tacaaccca 
      tatgggcgt gataggcca cacaacgaa agcgattac 
      cgaccatat ggccaagac aagcgtccg tatgaccaa 
      gaaccggga agtcgggat agggggcag gaatatccg 
      tttgatcca cggtacttt aaccgggag gaatatgga 
      gagaatatt gacccatat ggtacaagg agaaaaccc 
      tcagtctac aataatagc attccagac tattatgat 
      tacaacagg aataacacg ttttatgaa gtttttaat 
      ttgtttgag tcatcgccg cgttatggg attcacgga 
      aatctacta tttcaagat atgacacgg gaaattgcg 
      ccactcaat gaaaatctg caaacttac tcgaacttt 
      ttgggcagt tccatgaac attattatg ggggttagg 
      gtgtgtcag gtcaataga atgaccctc tgtgtgact 
      tcggacgtt gagcgaaac aaatgcgtg aagatgagg 
      acggcgttg aaagcccac ctcctcaag cccgaaatg 
      gactgctac aaaggccac tcgcagatc cactgcatg 
      caggccatc agatcgggc cttgccgac gtcgctgtg 
      ttggacgcc agcgacgtc tacaccgcc ggcctaaac 
      ttcgacctc atccccttc atgagcgag atgtacaac 
      ttaaacgaa cccgaatac tatgtcgtg gctgtgggc 
      aaagaatcc gacccctca accgaactg acatacctc 
      cgaggcaag tacacttgc cacggaggc atcaacact 
      gccgccggt tgggtgtac ccccttgca tttttaatc 
      agcaacggg tggattcgg tcctatggc tgcaacagt 
      atccgggct gcggccgag tacttttcg aagagttgc 
      gtcccgggg gccctaagc accgaatac aacaccgga 
      gtgccttac gataacatg tgccacctg tgccacggc 
      gccagcttc cggtattgc cgccgagac gcctcggag 
      gactactac gggcacacg ggcgccttc aggtgtctg 
      gtggaaggc ggcggtcaa gtcgccttc gtcacgcat 
      acgacagtg acgcagaat accaacggg aagagggaa 
      gagtggtgg gcgagggat aatctaaat gacgatttt 
      gaactactt tgtccagat ggtacgagg gccgagatc 
      aacgagtac gagaagtgc aatttgggc aaagtcaag 
      gcgaacgcg atcgtgacc cgcggcggt tacgggtac 
      aacgacacc cacgtgaat gcgtttatt aatctattt 
      ttgtacgcc cagaatttt tacggtcgg aagacggcc 
      gatgaattt agtttcagc atgttttcg tcaacgccg 
      ccgtacacc gatttgata ttccaagat gcgacgact 
      caattgaag gtcattccc ccggagaag cgacactat 
      gctgagtat ctcggaagc gacttcctg cgggcgcgg 
      aggatcgtt gattgtcac gccggaagt gcctctcta 
      agtgcccca atcacgcta cttacgacg attgtcgct 
      tttgttatt gttagttag
    mRNA sequence
    • >TC011919 RA
      cagtgttgt gctgggcgt gtttttgtc gctccgtgt 
      tatcaaatg tgttgattt gatattaaa gataatccc 
      aaaatgggc tttaaagat aggacttat ctttgcttt 
      gctctgctg atattgttt agtgttttt gtgataagt 
      gcaggccag aatttttgg aaaaccacg caatttaac 
      accgaggag cccgaagat aaacgcatt aagatagta 
      tggtgcaca ataagccgg gcagagcaa cggaaatgc 
      aaaaacttc gctatggca aacgaacgc gaccagatc 
      cgagtgggc tacgattat ttcaaaatc gagtgttta 
      caagcgttt gataaagac gagtgtatg actctccta 
      gaccaggag aaggccact ctgaccacc cttgacgcg 
      ggggaggtg ttcgtgggc gggcgctac aacagtctc 
      gtgcccatt gcccaggag gtcctagaa ggggggctc 
      cagcactat tacgccgtc gcggcggtc aaaaagggc 
      accctgccc catgtcaat tcgttgttt gatttgaga 
      ggggtcaag gcctgcttc gcaggcgtt gaaactttt 
      gctggatgg gttgtcccg atttacaca ttgatgaac 
      gagggtggg ttagatgtt gttgattgc aataatcac 
      gtcaagtcg gctacgaaa tactttggg ccgtcgtgc 
      gccgtcaac tgtctcacc aacaagtac aaccccatt 
      ggtgataat tccgacagg ttgtgccaa ctttgtatc 
      ggaaaaatc cccgggggg cggtgcact agctctgac 
      ccctactca ggttacgag ggggcgttc cgttgtctc 
      ttggaggcc ggagatgta gcatttgtc aaacataca 
      acgattccg tcacttgtg gccggagac aattttgtg 
      tcgatttcg aaagattcc ttcgaattg ctatgcaag 
      gacggcagt cggcgcccg attgacgac tacatgtcg 
      tgcaattgg ggcaaagtc ccgtcggac gcaatcgtg 
      accacttca gcctcttcc tttgaaaaa cgcaagatt 
      taccaaaaa ttcctcgaa aaggccgcg aaaatgtac 
      agttttggc aatcagaac aacgggacg catcgctac 
      gatgatcag aatcggaat tcttacgat gatcggcag 
      ccacagtat gatcagttc gggaacagg attgatcag 
      cctaactat gatcagtac ggagggagg ccgaggtac 
      aaaaggcag aattttggg gattttggg acgcaaaat 
      aggccccaa catgacccc aacatccca cagtatgag 
      gaaaaccgg ccgcagtat gaccgaaat aattacaac 
      ccatatggg cgtgatagg ccacacaac gaaagcgat 
      taccgacca tatggccaa gacaagcgt ccgtatgac 
      caagaaccg ggaagtcgg gataggggg caggaatat 
      ccgtttgat ccacggtac tttaaccgg gaggaatat 
      ggagagaat attgaccca tatggtaca aggagaaaa 
      ccctcagtc tacaataat agcattcca gactattat 
      gattacaac aggaataac acgttttat gaagttttt 
      aatttgttt gagtcatcg ccgcgttat gggattcac 
      ggaaatcta ctatttcaa gatatgaca cgggaaatt 
      gcgccactc aatgaaaat ctgcaaact tactcgaac 
      tttttgggc agttccatg aacattatt atgggggtt 
      agggtgtgt caggtcaat agaatgacc ctctgtgtg 
      acttcggac gttgagcga aacaaatgc gtgaagatg 
      aggacggcg ttgaaagcc cacctcctc aagcccgaa 
      atggactgc tacaaaggc cactcgcag atccactgc 
      atgcaggcc atcagatcg ggccttgcc gacgtcgct 
      gtgttggac gccagcgac gtctacacc gccggccta 
      aacttcgac ctcatcccc ttcatgagc gagatgtac 
      aacttaaac gaacccgaa tactatgtc gtggctgtg 
      ggcaaagaa tccgacccc tcaaccgaa ctgacatac 
      ctccgaggc aagtacact tgccacgga ggcatcaac 
      actgccgcc ggttgggtg taccccctt gcattttta 
      atcagcaac gggtggatt cggtcctat ggctgcaac 
      agtatccgg gctgcggcc gagtacttt tcgaagagt 
      tgcgtcccg ggggcccta agcaccgaa tacaacacc 
      ggagtgcct tacgataac atgtgccac ctgtgccac 
      ggcgccagc ttccggtat tgccgccga gacgcctcg 
      gaggactac tacgggcac acgggcgcc ttcaggtgt 
      ctggtggaa ggcggcggt caagtcgcc ttcgtcacg 
      catacgaca gtgacgcag aataccaac gggaagagg 
      gaagagtgg tgggcgagg gataatcta aatgacgat 
      tttgaacta ctttgtcca gatggtacg agggccgag 
      atcaacgag tacgagaag tgcaatttg ggcaaagtc 
      aaggcgaac gcgatcgtg acccgcggc ggttacggg 
      tacaacgac acccacgtg aatgcgttt attaatcta 
      tttttgtac gcccagaat ttttacggt cggaagacg 
      gccgatgaa tttagtttc agcatgttt tcgtcaacg 
      ccgccgtac accgatttg atattccaa gatgcgacg 
      actcaattg aaggtcatt cccccggag aagcgacac 
      tatgctgag tatctcgga agcgacttc ctgcgggcg 
      cggaggatc gttgattgt cacgccgga agtgcctct 
      ctaagtgcc ccaatcacg ctacttacg acgattgtc 
      gcttttgtt attgttagt tagtttttt atagcactt 
      tttctattt ttttatgta caaatttat tgtgaaccg 
      tccatttta acgagattt gtgtgaggt ccgtccatt 
      atctttatc gtccagata agggaacga aaatagtta 
      cgacgtaat taaaatatt taacgaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY