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Gene information
General information
TC030743 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11557448..11559771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030743
    iBeetle screen iB_09482

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 2

    mature egg not present - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030743 RA
      MSLFSAPTK STTPSSILK KTPSLKSTL NRSLKVNTP 
      TKVRFILPH SKKVRHILK DYIKHGNAK DYENLVCLI 
      RDAELFDDD ISSLLKEAT ECISIMKQE LRLFVEALL 
      SVDWIERNE NVVKEYQSF IANLVAAHN YHADFVINK 
      LVTLFFPPL TDPEWENGT PSKTDILKC QHVHSLLCE 
      LLRTVPLCN ELLIHALID HFPYMNKST HIHEYYIQN 
      LLWVICYQP TLRPNILHL IFSKLIIMD VSAPREEIA 
      KLEEEEIFM MDESKTNTT INLDCVHSM AHTLDVCLD 
      KVFNYIYSE CHEPGTNDL NWGKTKSLY QDILTVFDQ 
      SILPTYNTH HVQFVMFVL CSFKTTLTE AFFNYLWKK 
      VCNPNVASV IRQAAVNYI VSFIARATF VPLTMLKGT 
      LQQMAEWIH SYIATQDGL ECVNSDLRL HSVFYTVCQ 
      ALFYVISFR HKDLVNTKK NVVFLESLN LAKIVTSRL 
      NPLRVCQPA VVQNFAAIT RKYQLAYCY SIIEHNSRN 
      TLPTVYRDE KGFVTVSSN VLEACYPFD PFVLSRSSA 
      KVHPFYRDY KEVSFEVND SEMREIGDV DDFLENDFN 
      SKTQKFSYG TSPGFKFKG
    CDS sequence
    • >TC030743 RA
      atgtcttta ttctcggcc cccacgaag tcaacgacc 
      ccttcctcg atcctgaag aaaacccca agtctcaaa 
      tcgacatta aaccgctcc ttgaaagtg aacacaccc 
      actaaagtc cgatttatt ttaccacac tctaaaaaa 
      gtgcgccac atattgaag gactacatc aaacacgga 
      aatgcaaag gattatgaa aatttagtg tgcctaatt 
      cgcgatgcc gaactgttt gatgacgat atttcctca 
      ctgttgaag gaagcgact gagtgtatt tcaataatg 
      aagcaggag ttacgtctt tttgtagaa gcactcctt 
      tcagttgac tggatcgaa agaaacgaa aatgtagtt 
      aaggagtat cagtcattt attgcgaac ttagtcgct 
      gcacataac tatcacgca gattttgtg ataaataaa 
      ttagtcaca ttgtttttt ccccctcta acagaccct 
      gaatgggaa aatggaacc cccagtaaa acggatatt 
      ctgaagtgc caacacgtc cactccctc ctctgtgaa 
      cttctaaga acagtccct ttgtgcaac gaattgcta 
      atccacgcc ctgatcgac cacttccct tacatgaac 
      aaatcgacc cacatccac gaatactac atccagaac 
      ctcctctgg gtcatctgc taccagccc actctcaga 
      cccaacatc ctccactta attttctca aaactgatc 
      atcatggac gtgagtgcg ccccgcgag gaaatcgcg 
      aaacttgaa gaggaggaa attttcatg atggacgag 
      agcaaaaca aacactacg ataaatctg gactgcgtc 
      cattcaatg gcacacact ttggacgtt tgcctggat 
      aaagtgttc aattacatt tactccgag tgccatgag 
      ccgggcact aatgacttg aattggggc aaaaccaag 
      tctttatac caggatatt ttgacggtc tttgatcag 
      tcgatcctg ccaacttac aatactcat cacgtccag 
      tttgttatg tttgtgctg tgttccttt aaaacgacg 
      ttaacggag gcgtttttt aattacttg tggaagaag 
      gtgtgcaat ccgaacgtc gcgtcggtg attagacaa 
      gcggcggtt aattatatt gtcagtttt atagcgaga 
      gccactttc gtccccctt accatgttg aaggggact 
      ttgcaacaa atggccgaa tggatccac tcgtatatc 
      gccactcag gacggccta gagtgcgtc aattcggac 
      ctaaggctc cactctgtc ttctacaca gtgtgtcaa 
      gccctcttt tatgtgatt tcgttccgg cacaaggat 
      ttagtcaac acaaaaaaa aatgtcgta tttttggag 
      agtctgaac ttggcgaaa atcgtaacc agtcggtta 
      aacccgttg cgggtttgc caacctgca gtcgtgcaa 
      aatttcgct gctattacc cgaaagtac caactagcc 
      tattgttac tcaatcatt gaacacaat tcgaggaac 
      actttaccg actgtttat agagacgag aaaggtttt 
      gtgacagtt tcaagtaac gtcctagag gcgtgttac 
      ccgtttgat ccttttgtt ttatcaagg tcgagtgcg 
      aaagtgcac ccgttttat cgggactac aaggaggtg 
      agttttgag gtaaacgat tcagaaatg agggaaatt 
      ggggacgtt gacgatttc ctcgagaat gactttaac 
      tcaaaaacg cagaaattc tcatacggg acttcgcca 
      gggtttaaa tttaagggc taa
    mRNA sequence
    • >TC030743 RA
      cgcagacgt caaaccaca cgtttttct cctaacctg 
      taaaagatg tgatttaaa taacattgt gaaatactg 
      tttttattg attgactgt gactgaacc gttgcttta 
      atttatatc attatgtct ttattctcg gcccccacg 
      aagtcaacg accccttcc tcgatcctg aagaaaacc 
      ccaagtctc aaatcgaca ttaaaccgc tccttgaaa 
      gtgaacaca cccactaaa gtccgattt attttacca 
      cactctaaa aaagtgcgc cacatattg aaggactac 
      atcaaacac ggaaatgca aaggattat gaaaattta 
      gtgtgccta attcgcgat gccgaactg tttgatgac 
      gatatttcc tcactgttg aaggaagcg actgagtgt 
      atttcaata atgaagcag gagttacgt ctttttgta 
      gaagcactc ctttcagtt gactggatc gaaagaaac 
      gaaaatgta gttaaggag tatcagtca tttattgcg 
      aacttagtc gctgcacat aactatcac gcagatttt 
      gtgataaat aaattagtc acattgttt tttccccct 
      ctaacagac cctgaatgg gaaaatgga acccccagt 
      aaaacggat attctgaag tgccaacac gtccactcc 
      ctcctctgt gaacttcta agaacagtc cctttgtgc 
      aacgaattg ctaatccac gccctgatc gaccacttc 
      ccttacatg aacaaatcg acccacatc cacgaatac 
      tacatccag aacctcctc tgggtcatc tgctaccag 
      cccactctc agacccaac atcctccac ttaattttc 
      tcaaaactg atcatcatg gacgtgagt gcgccccgc 
      gaggaaatc gcgaaactt gaagaggag gaaattttc 
      atgatggac gagagcaaa acaaacact acgataaat 
      ctggactgc gtccattca atggcacac actttggac 
      gtttgcctg gataaagtg ttcaattac atttactcc 
      gagtgccat gagccgggc actaatgac ttgaattgg 
      ggcaaaacc aagtcttta taccaggat attttgacg 
      gtctttgat cagtcgatc ctgccaact tacaatact 
      catcacgtc cagtttgtt atgtttgtg ctgtgttcc 
      tttaaaacg acgttaacg gaggcgttt tttaattac 
      ttgtggaag aaggtgtgc aatccgaac gtcgcgtcg 
      gtgattaga caagcggcg gttaattat attgtcagt 
      tttatagcg agagccact ttcgtcccc cttaccatg 
      ttgaagggg actttgcaa caaatggcc gaatggatc 
      cactcgtat atcgccact caggacggc ctagagtgc 
      gtcaattcg gacctaagg ctccactct gtcttctac 
      acagtgtgt caagccctc ttttatgtg atttcgttc 
      cggcacaag gatttagtc aacacaaaa aaaaatgtc 
      gtatttttg gagagtctg aacttggcg aaaatcgta 
      accagtcgg ttaaacccg ttgcgggtt tgccaacct 
      gcagtcgtg caaaatttc gctgctatt acccgaaag 
      taccaacta gcctattgt tactcaatc attgaacac 
      aattcgagg aacacttta ccgactgtt tatagagac 
      gagaaaggt tttgtgaca gtttcaagt aacgtccta 
      gaggcgtgt tacccgttt gatcctttt gttttatca 
      aggtcgagt gcgaaagtg cacccgttt tatcgggac 
      tacaaggag gtgagtttt gaggtaaac gattcagaa 
      atgagggaa attggggac gttgacgat ttcctcgag 
      aatgacttt aactcaaaa acgcagaaa ttctcatac 
      gggacttcg ccagggttt aaatttaag ggctaatgt 
      gatactgat ttatgcaag atgttattt attaatttg 
      ataggaaaa taaagtttc tcaaaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY