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Gene information
General information
TC012535 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11058555..11059680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012535
    iBeetle screen iB_09481

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012535 RA
      MSLWNRLSH WLLTETGKK ACYYAAGAT TSVILLAHT 
      LPQTIFLSQ YEDLFHLYK HGFSVPLTE KVQKRFEKA 
      LDLLEVEHR DRHLYKPFA AYGFDIFSA GTSYSKFGV 
      RVGIPTHFF YDDESSVDK SSIKIRDES VIWESDEGK 
      LLLKSLVLS ENAQIYAMA REIKMRDTL KGMLDTVVA 
      VVSWSGMYG ISNTLNIKF DLYSKPRPV RIIMYCLVG 
      AFSFGSYVF CKDFSQVYY ETQIDKELK EKNPVFAEG 
      GKEFYNKIV ARNIALRKM LGKEGERAY TALGNDNYL 
      FRYRHVPVV QRKLFFEGE KA
    CDS sequence
    • >TC012535 RA
      atgtcgttg tggaaccgt ttatcccac tggttgttg 
      accgagacg ggcaaaaaa gcgtgttat tacgcggcc 
      ggtgcaacc acttctgtg attctcctg gcccacact 
      ttgccccaa accattttc ctgagccaa tacgaggac 
      cttttccac ctctacaag cacggcttt tcggtgccg 
      ttgacggaa aaggtccag aaacgcttt gaaaaggcc 
      ctggatttg cttgaagtg gagcaccgg gaccggcat 
      ttgtacaag cctttcgcg gcctatggc ttcgatatt 
      ttcagcgct ggcacgtct tacagcaag tttggggtc 
      agagtgggc atcccgaca cattttttc tacgacgac 
      gagtcgtca gttgacaag agttccatt aaaatcagg 
      gatgagtcg gttatttgg gagtccgat gaggggaag 
      ttgctgtta aaatcgctg gttttgtcg gaaaatgcc 
      caaatttac gccatggcg agggaaatc aaaatgaga 
      gacacgctg aaggggatg ctggacacg gttgtggcc 
      gtggtttcg tggtctggc atgtacgga atttccaac 
      actttgaac attaagttc gatttgtat tcaaaacct 
      agacctgta aggattatt atgtattgc ctggttggg 
      gctttctcg ttcgggagt tacgtcttc tgcaaagac 
      tttagccaa gtttattac gaaacgcaa attgataag 
      gaactgaag gaaaaaaat ccggttttt gctgaaggt 
      ggcaaggag ttttacaac aagattgtg gccagaaac 
      atcgccttg cggaaaatg ctagggaaa gagggagag 
      cgggcttat accgcccta gggaacgac aattatcta 
      tttaggtat aggcacgta cccgtagtt cagagaaag 
      ttgtttttc gagggcgaa aaagcctaa
    mRNA sequence
    • >TC012535 RA
      gttttccaa taaaaacta ttattaata accgaaaac 
      taagtccga ttgtgagcc gcatgacat ccaagtgtc 
      aacaaaagt gaggttatc tcacaaaac accgtcaca 
      attgttatg aagaaaact taattttta cgtaatttc 
      cgacatgtc gttgtggaa ccgtttatc ccactggtt 
      gttgaccga gacgggcaa aaaagcgtg ttattacgc 
      ggccggtgc aaccacttc tgtgattct cctggccca 
      cactttgcc ccaaaccat tttcctgag ccaatacga 
      ggacctttt ccacctcta caagcacgg cttttcggt 
      gccgttgac ggaaaaggt ccagaaacg ctttgaaaa 
      ggccctgga tttgcttga agtggagca ccgggaccg 
      gcatttgta caagccttt cgcggccta tggcttcga 
      tattttcag cgctggcac gtcttacag caagtttgg 
      ggtcagagt gggcatccc gacacattt tttctacga 
      cgacgagtc gtcagttga caagagttc cattaaaat 
      cagggatga gtcggttat ttgggagtc cgatgaggg 
      gaagttgct gttaaaatc gctggtttt gtcggaaaa 
      tgcccaaat ttacgccat ggcgaggga aatcaaaat 
      gagagacac gctgaaggg gatgctgga cacggttgt 
      ggccgtggt ttcgtggtc tggcatgta cggaatttc 
      caacacttt gaacattaa gttcgattt gtattcaaa 
      acctagacc tgtaaggat tattatgta ttgcctggt 
      tggggcttt ctcgttcgg gagttacgt cttctgcaa 
      agactttag ccaagttta ttacgaaac gcaaattga 
      taaggaact gaaggaaaa aaatccggt ttttgctga 
      aggtggcaa ggagtttta caacaagat tgtggccag 
      aaacatcgc cttgcggaa aatgctagg gaaagaggg 
      agagcgggc ttataccgc cctagggaa cgacaatta 
      tctatttag gtataggca cgtacccgt agttcagag 
      aaagttgtt tttcgaggg cgaaaaagc ctaataact 
      tgtaattta ttttgtaca tatgtttga ataaaccgc 
      tcgatttta g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY