Status
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Gene information
General information
TC012522 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10979325..10983909
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012522
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09479

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012522 RA
      MTNTTNASP PSPYVFKPF RYEEIKPNR KSRVPYPRS 
      GHRIGADSA NFYSFGGYN PLIGSDEAI SDEIWVQSY 
      PLFQELWRF NFARKEWTR YSNSHTLPL ELASNALVL 
      HKNVLMVYG GTGSPFGFR CSNQLFVCR VNDSEGLMD 
      EVHTTGQLP LPAYGQALI YHNDYLYTI GGTTGVQYT 
      CDIHRLNLK TLVWESVYI CRGIGEYEP TGRYRHEVG 
      FDKRNIYVL GGGTALLAF DFSTIPVFN LEKQIWYPQ 
      KSIRDTRYG VPQPRRCHG AVQITTEFG NQVFIAGGH 
      DGENVFDDL WRLDLQTFQ WTYFDKCRL PFPIYFHAA 
      AASPEGRLY IFGGICSSN DNDVRRSNC MYSTWLCIP 
      KLSEICWEA VLHYSPHIV KCRSEDLIN IGLPRHFVQ 
      RLGNNNPPI KREQ
    CDS sequence
    • >TC012522 RA
      atgactaac acgaccaac gccagccct ccctcgccc 
      tacgtcttc aagccgttc cggtacgag gaaatcaag 
      ccgaaccga aagagcaga gtgccttac ccccgcagc 
      ggccaccgc atcggcgcc gactccgcc aacttctac 
      tctttcggg ggctacaac cccctcata ggcagcgac 
      gaggccata agcgacgag atctgggtc cagtcctac 
      ccccttttc caggagctg tggcgcttc aacttcgcc 
      cggaaggag tggacgcgc tactccaac agccacacg 
      ttgcccctc gagctcgcc tccaacgcc ctggtgctg 
      cacaagaac gtcctgatg gtctacggg ggcaccggg 
      tcccccttt gggttccgc tgcagcaac cagctgttc 
      gtgtgtcgg gtcaacgac agcgagggg ctcatggac 
      gaggtgcac accacgggg cagctgccc ctgccggcc 
      tacggccag gccctcatc taccacaat gactacctc 
      tacaccatc gggggaacc accggagtt caatacaca 
      tgtgatatt cacaggtta aatctcaaa actctagtc 
      tgggaaagc gtctacatt tgccgcggg atcggcgaa 
      tacgaacct acaggtcgt taccgccac gaagtcggt 
      ttcgacaaa cgtaacatt tacgttctg gggggcgga 
      acggccctt ctcgccttc gatttctca acgattcca 
      gttttcaat ttagaaaag caaatctgg tacccgcag 
      aaatcaatc agggacacg cgatacggt gtgccccag 
      ccgcggcgc tgccacgga gccgtccag ataaccacc 
      gagtttggc aaccaggtg ttcatcgcg ggcggccac 
      gacggcgag aatgtgttc gacgacctc tggcggttg 
      gatttgcaa acgtttcag tggacttac ttcgacaag 
      tgtcgtttg ccgtttcct atttatttc cacgcggcg 
      gcggcttcg cccgaaggc agactttac atcttcggc 
      gggatttgc agtagcaac gataatgat gttagacgg 
      agtaattgc atgtattcg acgtggctg tgtatacct 
      aaactaagt gagatctgc tgggaagcc gtactgcac 
      tatagtccc cacattgtt aagtgtagg agcgaggat 
      ttgattaat atagggtta ccgagacat tttgtgcaa 
      aggttaggc aataataat ccgccgatt aaaagagaa 
      caataa
    mRNA sequence
    • >TC012522 RA
      ggtgtctaa aaaatcacg agcaaaaaa caaattgat 
      tcaattttg ctgactcaa gaatccgcc acaaaccag 
      ccacgtgaa tttaacggg catttaagt tttttggag 
      tcgttgccg ctggttggg aacccctgc tccaacgaa 
      acttgtttt cagtcccat tacgtcacc gaaacgtca 
      aaccagctg tggttgtgg aggtaacct tcaacttcg 
      ttgtgaaag aattgaatc ctttcaaaa tggttttat 
      ttgcgtgta aatccccgc gcagtgcgt acgatgttt 
      gctccaaat gtattcgaa gaccctcta ccaagttct 
      aaagaatct catcgattt ttttacaac aatgactaa 
      cacgaccaa cgccagccc tccctcgcc ctacgtctt 
      caagccgtt ccggtacga ggaaatcaa gccgaaccg 
      aaagagcag agtgcctta cccccgcag cggccaccg 
      catcggcgc cgactccgc caacttcta ctctttcgg 
      gggctacaa ccccctcat aggcagcga cgaggccat 
      aagcgacga gatctgggt ccagtccta ccccctttt 
      ccaggagct gtggcgctt caacttcgc ccggaagga 
      gtggacgcg ctactccaa cagccacac gttgcccct 
      cgagctcgc ctccaacgc cctggtgct gcacaagaa 
      cgtcctgat ggtctacgg gggcaccgg gtccccctt 
      tgggttccg ctgcagcaa ccagctgtt cgtgtgtcg 
      ggtcaacga cagcgaggg gctcatgga cgaggtgca 
      caccacggg gcagctgcc cctgccggc ctacggcca 
      ggccctcat ctaccacaa tgactacct ctacaccat 
      cgggggaac caccggagt tcaatacac atgtgatat 
      tcacaggtt aaatctcaa aactctagt ctgggaaag 
      cgtctacat ttgccgcgg gatcggcga atacgaacc 
      tacaggtcg ttaccgcca cgaagtcgg tttcgacaa 
      acgtaacat ttacgttct ggggggcgg aacggccct 
      tctcgcctt cgatttctc aacgattcc agttttcaa 
      tttagaaaa gcaaatctg gtacccgca gaaatcaat 
      cagggacac gcgatacgg tgtgcccca gccgcggcg 
      ctgccacgg agccgtcca gataaccac cgagtttgg 
      caaccaggt gttcatcgc gggcggcca cgacggcga 
      gaatgtgtt cgacgacct ctggcggtt ggatttgca 
      aacgtttca gtggactta cttcgacaa gtgtcgttt 
      gccgtttcc tatttattt ccacgcggc ggcggcttc 
      gcccgaagg cagacttta catcttcgg cgggatttg 
      cagtagcaa cgataatga tgttagacg gagtaattg 
      catgtattc gacgtggct gtgtatacc taaactaag 
      tgagatctg ctgggaagc cgtactgca ctatagtcc 
      ccacattgt taagtgtag gagcgagga tttgattaa 
      tatagggtt accgagaca ttttgtgca aaggttagg 
      caataataa tccgccgat taaaagaga acaataaat 
      actcatttc ccgaaggga aactggact ttatttatt 
      taaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY