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Gene information
General information
TC034377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10759347..10760680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034377
    iBeetle screen iB_09476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034377 RA
      MKNSSFHLA SEVATMKTV NEAQSSEIL 
      VVALIPSFS HFSIPFKLS KELAKRGHK 
      VTCINPYPQ KAPIKNYID VSVAENIET 
      MEEIKKQLF DMNKFSVIE NLTFIFDML 
      KTLVEKTMT NKNVQELLK SDKKFDVVI 
      IENFLNEAM FGLGHLFKA PVILISATA 
      ASDMNNYIF ANPAPTSYV PGSGGTLTK 
      HMNFWQRIE NLVTRSLDT RNKLCERSK
    CDS sequence
    • >TC034377 RA
      atgaaaaac tctagtttt catttggca agtgaggtg 
      gcaacaatg aaaactgta aatgaggcc caaagctcc 
      gaaattcta gttgttgcc ctcatccct tcattcagt 
      catttttcc ataccgttc aaactttcg aaagaattg 
      gcaaagcgg ggacacaaa gtgacttgc attaaccca 
      tatcctcag aaagcaccc atcaaaaac tacatcgat 
      gtctcggtt gccgaaaat atagaaacc atggaagaa 
      attaaaaaa caattattt gatatgaac aaatttagc 
      gttatcgaa aatctgacg tttattttt gatatgcta 
      aaaactcta gtcgagaaa accatgacg aataaaaat 
      gtccaagag ttgctaaaa tctgacaaa aaatttgat 
      gtcgtgata attgaaaat tttttgaac gaagctatg 
      ttcgggtta ggccacctg tttaaggct ccagtgatt 
      ctcatttct gcaactgcc gcaagcgac atgaacaat 
      tatattttt gcaaatccg gcccctact tcgtatgtt 
      cccgggtcg ggggggact ttaacaaaa catatgaat 
      ttctggcaa agaattgaa aatttggtc acgagaagc 
      cttgacacg cggaataaa ctttgcgag aggtcaaaa 
      taa
    mRNA sequence
    • >TC034377 RA
      aaatataaa acctttacc cttctatta atagaaaaa 
      aagttatct caaataata atcagtgag tgtgatgac 
      ttgatgagc aataaatta aagataaga tcttcttaa 
      aatgatgaa aaaggaatg aaaaactct agttttcat 
      ttggcaagt gaggtggca acaatgaaa actgtaaat 
      gaggcccaa agctccgaa attctagtt gttgccctc 
      atcccttca ttcagtcat ttttccata ccgttcaaa 
      ctttcgaaa gaattggca aagcgggga cacaaagtg 
      acttgcatt aacccatat cctcagaaa gcacccatc 
      aaaaactac atcgatgtc tcggttgcc gaaaatata 
      gaaaccatg gaagaaatt aaaaaacaa ttatttgat 
      atgaacaaa tttagcgtt atcgaaaat ctgacgttt 
      atttttgat atgctaaaa actctagtc gagaaaacc 
      atgacgaat aaaaatgtc caagagttg ctaaaatct 
      gacaaaaaa tttgatgtc gtgataatt gaaaatttt 
      ttgaacgaa gctatgttc gggttaggc cacctgttt 
      aaggctcca gtgattctc atttctgca actgccgca 
      agcgacatg aacaattat atttttgca aatccggcc 
      cctacttcg tatgttccc gggtcgggg gggacttta 
      acaaaacat atgaatttc tggcaaaga attgaaaat 
      ttggtcacg agaagcctt gacacgcgg aataaactt 
      tgcgagagg tcaaaataa caagatttg gtgtaaaaa 
      attgtattt attttttac cattttcga ctttttttc 
      ttgtcactt ttcactttg ttgagttag aaacacaat 
      tattatgtt gaggaatta cgtcaaaga taatccaag 
      aatcactaa cttgataaa attacatgg tattagcta 
      agctggtta agatgagat gagcaagaa tttctttga 
      tttcgaatg atttgcaag tttttcgcg ttcttttgc 
      gaattttat ctaaaaaaa ttacgaatt agttcaaaa 
      atgctaact tttttgtcc atttaaatg ctagtgtat 
      tttataata aatgacact cattcga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY