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Gene information
General information
TC011979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9377877..9378912
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011979
    iBeetle screen iB_09472

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4

    tropharium potentiallysize decreased - Number of animals showing the phenotype: 4

    tropharium potentiallybent - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011979 RA
      MMGDQFRKV EQYSPKSSP RGARSPVVS 
      RQDSSGTLK TTILLGKNP SIVPSGPFY 
      LMKEPPGES ELTGATNLM AYYGLEHSY 
      SKFSGKKLK EQLSSFLPT LPGVIDSPG 
      QHDNSSLRS VIEKPPIGG KELLPLTSI 
      QLDGFRLHP GALPEQYRY ANAAPMKKH 
      KNKHKKHKH KDGGLPSQE TTITDAGSD 
      THEKKHKKQ KRHDEDKER KKRKKEKKR 
      KKQKHSPEH SGGLTPSQH SNS
    CDS sequence
    • >TC011979 RA
      atgatgggc gatcagttt cgtaaagtg gaacaatat 
      tcgcccaaa tcttcgcct agaggggct cgttcccct 
      gtcgtttca cgtcaggat tcgtcagga acattaaaa 
      acgacaatt ttgttagga aagaaccct tcaattgtc 
      cctagtgga cctttctat ttgatgaaa gagcctcca 
      ggagagtcg gaactaaca ggagcgacg aatttgatg 
      gcctattat ggccttgag cactcttac agcaaattc 
      agtgggaaa aaactcaag gaacagtta tcttcgttt 
      ttgccaact ttgcctggc gttattgac tctcctgga 
      cagcatgat aacagttcg ctaagatca gtgattgag 
      aagcctcca attgggggc aaggagttg ttaccattg 
      actagcatc caactggat gggtttaga ctacaccct 
      ggggcgctc cctgaacaa taccgctac gcaaatgcg 
      gcgcctatg aaaaagcat aaaaataaa cacaaaaaa 
      cacaaacac aaagatggg gggcttcca agtcaggag 
      actaccatt accgatgct ggaagtgat acacacgag 
      aagaagcat aaaaaacag aaacgacac gacgaagat 
      aaagaacgg aagaagagg aaaaaggaa aagaagaga 
      aaaaagcag aaacacagt cctgaacac agtgggggc 
      ctaacgccc agccaacac tcaaattcg tga
    mRNA sequence
    • >TC011979 RA
      aaaattacc agggtaaca attgatttg tttgtttgt 
      ttgacgatt ttgtgttgg tacgtgatg ctgaagtga 
      catttccca tttccagct gcatcgttg ttgcaaaac 
      gatgaagtt gtaagatga tgggcgatc agtttcgta 
      aagtggaac aatattcgc ccaaatctt cgcctagag 
      gggctcgtt cccctgtcg tttcacgtc aggattcgt 
      caggaacat taaaaacga caattttgt taggaaaga 
      acccttcaa ttgtcccta gtggacctt tctatttga 
      tgaaagagc ctccaggag agtcggaac taacaggag 
      cgacgaatt tgatggcct attatggcc ttgagcact 
      cttacagca aattcagtg ggaaaaaac tcaaggaac 
      agttatctt cgtttttgc caactttgc ctggcgtta 
      ttgactctc ctggacagc atgataaca gttcgctaa 
      gatcagtga ttgagaagc ctccaattg ggggcaagg 
      agttgttac cattgacta gcatccaac tggatgggt 
      ttagactac accctgggg cgctccctg aacaatacc 
      gctacgcaa atgcggcgc ctatgaaaa agcataaaa 
      ataaacaca aaaaacaca aacacaaag atggggggc 
      ttccaagtc aggagacta ccattaccg atgctggaa 
      gtgatacac acgagaaga agcataaaa aacagaaac 
      gacacgacg aagataaag aacggaaga agaggaaaa 
      aggaaaaga agagaaaaa agcagaaac acagtcctg 
      aacacagtg ggggcctaa cgcccagcc aacactcaa 
      attcgtgaa gatctttgg attgtgaat aattccaac 
      ttaccctag gtttaagat ctattatta tttattgtt 
      tattatttt tgaaaataa acagactgt tatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY