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Gene information
General information
TC011981 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9363575..9369852
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011981
    iBeetle screen iB_09471

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011981 RA
      MPTNNQVQD CGTLIVQVA QVTADVLIL LFQVLYAFI 
      LSIVTLVVR KQPKSVRGE IVLITGAGH GIGKELAFL 
      YASQGATVV IWDINEKNG TQTVKEIEQ LGHPKAHFF 
      LCDVSNREN VLTVAKDVQ QKVGDVTIL INNAGIMPT 
      HPLLEHSKD EIQRIMDIN VMAHFWTLE AFLPVMKKH 
      NYGHIVSLS STAGIFGIP NLVPYCCSK YAVRGLMEA 
      LYQECRADK KNEIKFTSI YPYMVDTGL CKKPIIKFQ 
      NLMPLVNPK EAAAEIMDA QRRDLNEIT IPRYIFYAH 
      NLCRMLPLK AQLFIFDFV GVGLESDLS
    CDS sequence
    • >TC011981 RA
      atgccgaca aacaatcaa gtgcaagat tgtggtacg 
      ctcattgtg caagtagct caagtcact gcagacgtc 
      ctgatatta ctgttccaa gttttgtac gcgtttatt 
      ttaagtata gtgacttta gtagtgcgg aagcagcca 
      aagtcggtt cggggcgaa atcgttttg ataacaggt 
      gctgggcat ggcattggc aaagaattg gcctttttg 
      tacgcctcg caaggtgcc actgttgtc atttgggac 
      attaacgag aaaaatggt acccaaacc gtcaaagaa 
      atcgaacag cttggtcac cccaaagct catttcttc 
      ctgtgtgac gtttccaac cgcgaaaac gtcctaact 
      gtggccaaa gacgtccaa caaaaggtg ggcgatgtc 
      acgattctc atcaacaat gctgggatc atgccaaca 
      caccctttg ctagagcat tctaaagac gaaatccag 
      aggatcatg gacatcaac gtcatggcg cacttttgg 
      acgctcgag gctttcctc cctgtgatg aaaaaacac 
      aattacgga cacattgtc tccctttcg tcaacagca 
      ggaatattc gggataccg aacttggtg ccttattgt 
      tgctcgaaa tacgctgtt cgaggcttg atggaagcc 
      ctctaccag gagtgcaga gcagacaaa aagaacgaa 
      atcaagttc acttcgatt tacccctac atggttgat 
      acagggttg tgcaaaaaa cccatcatc aagttccaa 
      aacttgatg cctttggtg aatcccaaa gaagcagcg 
      gctgaaatt atggacgca caaaggagg gatttgaac 
      gaaattaca attccgaga tacattttc tacgcccat 
      aacttgtgc cggatgctt cccctcaag gctcagttg 
      ttcattttc gactttgtt ggagttgga ttggagtca 
      gacttgtcg taa
    mRNA sequence
    • >TC011981 RA
      ccatgaaat ttaccttat ttttctatt tttgtacaa 
      tttttttca ttcacttga atatcacac ctacacctg 
      cctctcaaa cccaagatg atatttcta aattaccat 
      ttctcaaaa gaatagaaa aattcttag attcgctat 
      tatcccaat ttcctttgg aaagctcaa aggccaaga 
      gtagcaatt ttttttcac aacctttgg cactacttt 
      acagccaga aaaaggaac ctccccaag gcttatcat 
      cttattttt tttcacaat ctgatccat ctaaacaaa 
      tgcaaggcc gctcattgg gaagtaccg cttttacgt 
      ggcttgttc ccggggggc gcggtacgt aaaataatc 
      agtttaaac attcccatt aataaattc ctgaatatc 
      aacagcaat ttacgattg ctacacaaa agcgataca 
      aatcaagga catgataac aggtaaatt cactgaaat 
      ttgggataa cttatcgca gattcccgg taaattgtt 
      aggagctca cgagaaaag gtcattaag cagtgatct 
      gatcaagat cttacctca aatttttta atgaactgg 
      acaaagtca gggattgaa ggactggct tattaagtc 
      ttgaatgaa gtacttaaa tatttattg ttgataaat 
      aaatttgtt gcattatgt tctattgtt ccttaacca 
      atttgttcc tccgaattt ttcatttga aatctcaac 
      tataagtgc cccaataat cgataaatc aaacatttg 
      ataacattg ttaagcatt ttacggctc ccattttga 
      cttaccgtt acaaaatag ataaatcca tctaatctc 
      tatttatgc actgcttcg tcaggtccc aaaaattgt 
      aattcattg ttttcacac acgcagtgc ttttagtcc 
      aataataaa atatttgac cgataaatc aagaactta 
      tcttaaggt gagacattt tcgattcgt caaaggtcg 
      attaaaaaa tattaaacc gacccaatc gaggctgga 
      gataacact tgaatcatt atctaacaa agttaaagc 
      attcaaaac aaatctacg tcttagttc atgaagctt 
      tgctggaag aagtgccaa taagatgcc gacaaacaa 
      tcaagtgca agattgtgg tacgctcat tgtgcaagt 
      agctcaagt cactgcaga cgtcctgat attactgtt 
      ccaagtttt gtacgcgtt tattttaag tatagtgac 
      tttagtagt gcggaagca gccaaagtc ggttcgggg 
      cgaaatcgt tttgataac aggtgctgg gcatggcat 
      tggcaaaga attggcctt tttgtacgc ctcgcaagg 
      tgccactgt tgtcatttg ggacattaa cgagaaaaa 
      tggtaccca aaccgtcaa agaaatcga acagcttgg 
      tcaccccaa agctcattt cttcctgtg tgacgtttc 
      caaccgcga aaacgtcct aactgtggc caaagacgt 
      ccaacaaaa ggtgggcga tgtcacgat tctcatcaa 
      caatgctgg gatcatgcc aacacaccc tttgctaga 
      gcattctaa agacgaaat ccagaggat catggacat 
      caacgtcat ggcgcactt ttggacgct cgaggcttt 
      cctccctgt gatgaaaaa acacaatta cggacacat 
      tgtctccct ttcgtcaac agcaggaat attcgggat 
      accgaactt ggtgcctta ttgttgctc gaaatacgc 
      tgttcgagg cttgatgga agccctcta ccaggagtg 
      cagagcaga caaaaagaa cgaaatcaa gttcacttc 
      gatttaccc ctacatggt tgatacagg gttgtgcaa 
      aaaacccat catcaagtt ccaaaactt gatgccttt 
      ggtgaatcc caaagaagc agcggctga aattatgga 
      cgcacaaag gagggattt gaacgaaat tacaattcc 
      gagatacat tttctacgc ccataactt gtgccggat 
      gcttcccct caaggctca gttgttcat tttcgactt 
      tgttggagt tggattgga gtcagactt gtcgtaaac 
      ttgttatgt ttttttatt gttttgtta gtatagaag 
      tgtagataa tgaccagtg ataataaaa ttattatta 
      atag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY