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Gene information
General information
TC034869 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7804470..7810419
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034869
    iBeetle screen iB_09470

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034869 RA
      MMVEEAELE IYNEIINKG CCKRCVLRY LGQCKTLLT 
      FEHPNTCLV NFGYMDPIE EFEEERKAK IRKSNPCSV 
      CLGLLQDPA IEEMFACEG LNNISEYLS QTFVAYITF 
      PTCILIRDH SMKLHLKRL FPNRFDCSK VIKVNNAWR 
      YAVEERLSQ TLKKSYSHL SKLTLHFYT KYQLEDDEL 
      EAVQRVLKN LPKNNLSKH CVYNMLETI SDNEFGNLV 
      NVPPKVPLY AVTLDTLKF FSEAIHLIG NYLKYSRDL 
      FQVQNPFNT ASLDFSIET IIVDAVRNV ASDFKDAVF 
      KASGFDDNN IRVLGSGRT FHLQLNDPK FESVSKKQC 
      QVIEGIIKR SKLMAVRNL RECDKRDIC ILLDNEQRG 
      ARSYKALCM VYKCKNIDY CVNAVNMYE SLNVCQKIP 
      LKVFHKRKF YKKRRKIFQ IKARKLKGN LVELDLCTQ 
      CGFSVPEFV NGDLGRTKP SLCDIMNAK VDVLAVDIV 
      DFQPALHCD GEEDVII
    CDS sequence
    • >TC034869 RA
      atgatggtg gaagaagca gaactcgaa atatacaat 
      gaaataata aataaaggg tgttgcaaa cgatgtgtt 
      ttgcggtat ttggggcaa tgcaaaact ctgctgact 
      ttcgaacac ccaaacact tgcctagtg aattttgga 
      tacatggac cctattgag gaatttgaa gaagaacga 
      aaggcgaaa attcgtaaa agtaacccg tgttcagtt 
      tgcttgggc cttttgcaa gatcccgcc atcgaggaa 
      atgtttgca tgtgaaggc ttgaataac atttctgag 
      tacctgagt cagactttt gtggcctat attactttc 
      ccgacatgc attttaata agggatcat agtatgaaa 
      ctgcacttg aagcgctta tttccaaat cggtttgac 
      tgttccaaa gttataaaa gtgaacaat gcctggcga 
      tatgctgtt gaagaacga ctgtctcaa accttgaaa 
      aagtcttac agtcattta tcgaaatta actttgcat 
      ttttacacc aagtatcag ctggaagat gatgaactg 
      gaggcagta cagcgtgtt ctgaaaaac ctcccaaaa 
      aacaattta agtaaacac tgcgtttac aacatgctg 
      gagacaata tcagataat gaatttgga aacctggtc 
      aacgtcccc cccaaagtg cccctttat gccgtaacg 
      ctggacaca ttgaaattt ttctcagaa gctattcat 
      ctaatagga aattatttg aaatattct agggattta 
      tttcaagtc cagaatccg ttcaatact gcttcacta 
      gattttagc atagaaaca ataattgta gatgcagta 
      agaaacgtg gcaagtgat ttcaaagac gccgtcttt 
      aaagcatct ggttttgac gataacaac attagagtc 
      cttggaagc ggcagaacc ttccacttg cagctaaat 
      gaccctaag tttgagtca gtttcgaaa aaacagtgc 
      caagtaatt gaaggaatc attaagcgt agcaaactt 
      atggctgtg cgaaatttg cgggaatgc gacaagcga 
      gacatttgc atcctcttg gacaacgaa caacgcggt 
      gccaggagt tataaagcg ttgtgtatg gtgtacaaa 
      tgtaagaat atcgactat tgtgtaaat gcggtaaac 
      atgtatgaa tctttgaac gtttgccaa aaaattcca 
      ctaaaggtc tttcataag cggaaattt tataaaaag 
      cgaagaaag atatttcag attaaagct cggaaattg 
      aaaggtaat ttggttgag ttagacttg tgtacacag 
      tgcggtttc agcgtccct gaattcgtc aatggggac 
      cttggaagg actaaacct tcgctctgt gatatcatg 
      aacgctaaa gttgacgtt ctggcggtg gatattgtt 
      gatttccag cccgcactg cattgcgat ggagaggag 
      gacgtaata atttag
    mRNA sequence
    • >TC034869 RA
      cgacttgtc aaacaccat gatggtgga agaagcaga 
      actcgaaat atacaatga aataataaa taaagggtg 
      ttgcaaacg atgtgtttt gcggtattt ggggcaatg 
      caaaactct gctgacttt cgaacaccc aaacacttg 
      cctagtgaa ttttggata catggaccc tattgagga 
      atttgaaga agaacgaaa ggcgaaaat tcgtaaaag 
      taacccgtg ttcagtttg cttgggcct tttgcaaga 
      tcccgccat cgaggaaat gtttgcatg tgaaggctt 
      gaataacat ttctgagta cctgagtca gacttttgt 
      ggcctatat tactttccc gacatgcat tttaataag 
      ggatcatag tatgaaact gcacttgaa gcgcttatt 
      tccaaatcg gtttgactg ttccaaagt tataaaagt 
      gaacaatgc ctggcgata tgctgttga agaacgact 
      gtctcaaac cttgaaaaa gtcttacag tcatttatc 
      gaaattaac tttgcattt ttacaccaa gtatcagct 
      ggaagatga tgaactgga ggcagtaca gcgtgttct 
      gaaaaacct cccaaaaaa caatttaag taaacactg 
      cgtttacaa catgctgga gacaatatc agataatga 
      atttggaaa cctggtcaa cgtcccccc caaagtgcc 
      cctttatgc cgtaacgct ggacacatt gaaattttt 
      ctcagaagc tattcatct aataggaaa ttatttgaa 
      atattctag ggatttatt tcaagtcca gaatccgtt 
      caatactgc ttcactaga ttttagcat agaaacaat 
      aattgtaga tgcagtaag aaacgtggc aagtgattt 
      caaagacgc cgtctttaa agcatctgg ttttgacga 
      taacaacat tagagtcct tggaagcgg cagaacctt 
      ccacttgca gctaaatga ccctaagtt tgagtcagt 
      ttcgaaaaa acagtgcca agtaattga aggaatcat 
      taagcgtag caaacttat ggctgtgcg aaatttgcg 
      ggaatgcga caagcgaga catttgcat cctcttgga 
      caacgaaca acgcggtgc caggagtta taaagcgtt 
      gtgtatggt gtacaaatg taagaatat cgactattg 
      tgtaaatgc ggtaaacat gtatgaatc tttgaacgt 
      ttgccaaaa aattccact aaaggtctt tcataagcg 
      gaaatttta taaaaagcg aagaaagat atttcagat 
      taaagctcg gaaattgaa aggtaattt ggttgagtt 
      agacttgtg tacacagtg cggtttcag cgtccctga 
      attcgtcaa tggggacct tggaaggac taaaccttc 
      gctctgtga tatcatgaa cgctaaagt tgacgttct 
      ggcggtgga tattgttga tttccagcc cgcactgca 
      ttgcgatgg agaggagga cgtaataat ttaggattt 
      ctaactttt atttttgta caaagaaga attaaatac 
      taatttata aaagttgta aacacgtga taataaacg 
      agagaaccc ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY