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Gene information
General information
TC010662 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8230041..8235905
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010662
    iBeetle screen iB_09468

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010662 RA
      MWKSSKMDP VNEKNNIFH DLLEACKYS IPGSRLSYS 
      LRNRLEKYP PNVRREIVN RVKVGCSPL FLACKKGQT 
      EIVEYLITV CQADIEQRG LYEVADDRS THVVTPLWC 
      AAVSGKLSV VEILLRHNA DINAVSDTG STPVRSACF 
      MTHFDIVKY LVEHGADIN RPNYNGGTC LINSVQSAP 
      LCDFLLKHG ADVNARDIQ NKTALHYAI QEHRLETTK 
      LLLKNGANY NAKSRYGDD ALQMACLKG AARIFDYLT 
      VNICYSSDK LANAHELMG ATFLEEHND AVVALFHWR 
      EGLACRLNH GLLPKQPVQ QPKEGYRFQ REFETLEDL 
      DNIATDLDL IRTQSLIIA ERILGPHHK DTIFRLMYR 
      GAAYADMLR YQRCIDLWR RALEIRVEK DSILYTDTC 
      FTAQALVRQ MVDYNEKTV PKNEDDAKQ RFHDVVSTF 
      KLITKDILG IRQLLTIRP VYKRQIDFF DKILKCVTH 
      LIYLMIETA KTSEQIETV VKLIGNLVK MDPRSVLTE 
      DSLLHLCVS KCNTIRSSY FVDDDTIVI FPKPSVIKF 
      LLECGAHVN AKNELGSTP LHIATEPDN YSDWLIKLL 
      LQYGAHIDQ PNSRGICPS DYISDVSKR SEVVDVHIL 
      DYINLKCLC ATVISKFQI PYKNQIPKT LEFFVKLHE 
      P
    CDS sequence
    • >TC010662 RA
      atgtggaaa tcatcgaaa atggaccct gttaacgaa 
      aaaaacaat atatttcac gacctcctc gaggcctgc 
      aaatattcg atcccaggt tcgcgtctg tcctattct 
      ttgcgaaac cgtctggag aagtatccc ccgaacgtg 
      cgtcgcgaa atcgtaaat cgagtgaaa gtgggttgt 
      tccccctta tttctagcc tgcaaaaag ggccaaaca 
      gaaatcgtc gaatatctc ataacagtg tgtcaggct 
      gatatcgag caacgcggg ttgtacgaa gtggcggac 
      gataggtcg acgcacgtc gtgaccccc ctttggtgc 
      gccgcagtt tccgggaaa ttatcagta gttgagatt 
      ttgctccgc cacaatgct gatatcaac gctgtctcg 
      gacacgggc tcaaccccc gtgcgctcc gcctgcttc 
      atgacacat tttgacatt gtcaagtat ctagtcgag 
      cacggggcc gatatcaac cgccccaat tacaacggg 
      ggcacttgt ctcatcaac tcggtacag agtgccccc 
      ctgtgcgac tttctcctg aagcacgga gctgacgtc 
      aacgcacgc gacatccag aacaaaact gccctacat 
      tacgcgatc caggaacac agacttgaa acgaccaaa 
      ttgctgctc aaaaatggg gctaactac aacgccaag 
      tcacgttac ggtgacgac gcgctccaa atggcttgt 
      ctcaagggg gctgcccgc attttcgac tacctgact 
      gtaaatatc tgctactct agtgataag ctcgcaaac 
      gcgcatgaa ttaatgggg gccacgttc ctcgaagag 
      cacaacgac gccgtcgtt gcgttgttc cactggcgg 
      gagggctta gcttgtcgt ttaaaccac ggcctgttg 
      cccaaacaa cccgtgcaa caacccaag gaggggtac 
      cgtttccaa cgcgagttt gagactttg gaggacttg 
      gataatatc gcgactgat ttggatttg attcggacg 
      cagagtttg atcattgct gagcgaatt ttggggccg 
      catcacaag gacacgatt ttccgtttg atgtacagg 
      ggggctgcc tatgccgac atgttgagg taccagcgt 
      tgcatcgat ttgtggcgg cgggccttg gagatcagg 
      gttgagaaa gactcgatt ttgtataca gatacctgt 
      tttaccgcc caggcttta gtcagacaa atggtcgat 
      tataatgaa aaaacggtg ccgaaaaac gaagatgat 
      gcgaaacag aggttccac gacgttgta tccacattt 
      aaattgatt acgaaagac atcttagga ataagacaa 
      ctcttaact attaggcca gtatataaa cgtcaaatt 
      gactttttc gataagatc cttaagtgc gtgacccat 
      ttgatttat ttaatgatc gaaactgcg aaaactagt 
      gaacaaatt gagactgtt gtgaaatta ataggcaat 
      ttagtcaaa atggaccca agaagtgtc ctcacagaa 
      gactcgtta cttcaccta tgtgtctca aagtgtaat 
      acaatacga tctagttac ttcgtagac gacgataca 
      atcgtgata ttcccaaag cctagcgtg attaaattc 
      ttattggag tgtggcgct cacgtgaac gccaagaac 
      gagttaggc tccaccccc ttacacatc gccacagag 
      cccgacaac tactctgat tggctgatc aagttgctg 
      ctgcaatac ggagctcat atagatcaa ccgaattca 
      cgtggtatt tgtccgtcg gactacatc tctgacgta 
      agcaaacgc tcagaagtt gttgacgta cacattttg 
      gattatatt aatttgaaa tgtttatgc gctacggtc 
      attagtaaa tttcaaata ccgtataaa aatcagata 
      cccaaaacg ctcgaattt ttcgtcaaa ttacacgag 
      ccgtag
    mRNA sequence
    • >TC010662 RA
      gtgaggttg gcagcacgg tgaaagttt atcccgacc 
      tgtcaacgg tggtgtcaa aataaaaac aattattta 
      aatattgca aacagcttt aaaagatgt ggaaatcat 
      cgaaaatgg accctgtta acgaaaaaa acaatatat 
      ttcacgacc tcctcgagg cctgcaaat attcgatcc 
      caggttcgc gtctgtcct attctttgc gaaaccgtc 
      tggagaagt atcccccga acgtgcgtc gcgaaatcg 
      taaatcgag tgaaagtgg gttgttccc ccttatttc 
      tagcctgca aaaagggcc aaacagaaa tcgtcgaat 
      atctcataa cagtgtgtc aggctgata tcgagcaac 
      gcgggttgt acgaagtgg cggacgata ggtcgacgc 
      acgtcgtga ccccccttt ggtgcgccg cagtttccg 
      ggaaattat cagtagttg agattttgc tccgccaca 
      atgctgata tcaacgctg tctcggaca cgggctcaa 
      cccccgtgc gctccgcct gcttcatga cacattttg 
      acattgtca agtatctag tcgagcacg gggccgata 
      tcaaccgcc ccaattaca acgggggca cttgtctca 
      tcaactcgg tacagagtg cccccctgt gcgactttc 
      tcctgaagc acggagctg acgtcaacg cacgcgaca 
      tccagaaca aaactgccc tacattacg cgatccagg 
      aacacagac ttgaaacga ccaaattgc tgctcaaaa 
      atggggcta actacaacg ccaagtcac gttacggtg 
      acgacgcgc tccaaatgg cttgtctca agggggctg 
      cccgcattt tcgactacc tgactgtaa atatctgct 
      actctagtg ataagctcg caaacgcgc atgaattaa 
      tgggggcca cgttcctcg aagagcaca acgacgccg 
      tcgttgcgt tgttccact ggcgggagg gcttagctt 
      gtcgtttaa accacggcc tgttgccca aacaacccg 
      tgcaacaac ccaaggagg ggtaccgtt tccaacgcg 
      agtttgaga ctttggagg acttggata atatcgcga 
      ctgatttgg atttgattc ggacgcaga gtttgatca 
      ttgctgagc gaattttgg ggccgcatc acaaggaca 
      cgattttcc gtttgatgt acagggggg ctgcctatg 
      ccgacatgt tgaggtacc agcgttgca tcgatttgt 
      ggcggcggg ccttggaga tcagggttg agaaagact 
      cgattttgt atacagata cctgtttta ccgcccagg 
      ctttagtca gacaaatgg tcgattata atgaaaaaa 
      cggtgccga aaaacgaag atgatgcga aacagaggt 
      tccacgacg ttgtatcca catttaaat tgattacga 
      aagacatct taggaataa gacaactct taactatta 
      ggccagtat ataaacgtc aaattgact ttttcgata 
      agatcctta agtgcgtga cccatttga tttatttaa 
      tgatcgaaa ctgcgaaaa ctagtgaac aaattgaga 
      ctgttgtga aattaatag gcaatttag tcaaaatgg 
      acccaagaa gtgtcctca cagaagact cgttacttc 
      acctatgtg tctcaaagt gtaatacaa tacgatcta 
      gttacttcg tagacgacg atacaatcg tgatattcc 
      caaagccta gcgtgatta aattcttat tggagtgtg 
      gcgctcacg tgaacgcca agaacgagt taggctcca 
      cccccttac acatcgcca cagagcccg acaactact 
      ctgattggc tgatcaagt tgctgctgc aatacggag 
      ctcatatag atcaaccga attcacgtg gtatttgtc 
      cgtcggact acatctctg acgtaagca aacgctcag 
      aagttgttg acgtacaca ttttggatt atattaatt 
      tgaaatgtt tatgcgcta cggtcatta gtaaatttc 
      aaataccgt ataaaaatc agataccca aaacgctcg 
      aatttttcg tcaaattac acgagccgt agatttttt 
      cgaatgacg aaccgcaaa gactcgaaa aagtgatat 
      tctaattta agtattttt atatataaa atagtaacc 
      tatttattg agagatacg tatttattg ttttgtgtg 
      ggcggggct tgcccgaag tattgcata aataaaact 
      agaactgac atgacagta attcggctt cagccaagt 
      ccccaatga agttttgtt cctaagtag gtgttgcta 
      atttgatta a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY