Status
Please wait ...
Gene information
General information
TC012008 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8722292..8725532
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012008
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09466

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012008 RA
      MDWSLWRPR EETLATIKE LLQDALIPD SEVQKDVQT 
      KLKNLEIVD DFTYYLLYI VGQAHFSEE IRSLSGILL 
      KNNIAAVYN KLPEDSIIK IRQLCLMLL RDPCRDVRT 
      SISNVIYTL AKYNLNTWP ELIPFLVKS FETPDEYSE 
      VALTTLFSV CEEMMNLEK SEEEICTIT KEVLPKFVD 
      CLVDEKCDK KQSIIKLIN QFLQDHYKV MTQSIDLTR 
      YLRNVIQLA DTDDLDMQK YICHTFVMY IEYREECLL 
      PHLHDVIMY LLVKTQHED LEVALGACE FWLAATKLA 
      NCKELLTPY IDKLVPVLL KNMKYSSFE LIALKDTLR 
      VEEQNDDPS KETFPFHMH DKRITRDDD TFSNEEGDF 
      LGNCDDVDD FYVGWTLRK CSAASLDAI AVKFGEDIL 
      PLMIPFLNE LLYHQDFLI KESAILALG AISEGCING 
      LKPHLPYLV QYLIHSMND DHSMVRVIT CWTLSRYVC 
      WIINSQPSH SVYFIPVMT ILLKHFMDE NKRVQRAAI 
      SAFCVFQEE AQMQLIPYI DLILEGFQL GFQKFNYRS 
      LYLLYDAIN VLALSVGSE LSKPEYIEK LMPPLIQKL 
      NEYNNYSDD QFIAVLECL ANIIPSLDA GFLPYSEVL 
      YSQCMEIIM DTFMADANF QENLNGFDP PDKEPMHVA 
      LDVLYSMAV GLKSYFYKY VANSNLVCL LYTTMQDCS 
      FLIRQSSIA LYGELVLIC YPYLSSSVD DYIKLIIKN 
      LDECYEGVC KNAAWVIGK LTTVMGAEI RPYVPEILA 
      AFINISRNP TVSRAMHQT VSVSLCTLC CVCPDIALT 
      DADTVLKNC CLSLRSLKD SDEKDLAFR GLCQVVVRH 
      PDFCQNNFM YFCDAVASW NSVKPDLKE MIKNILVSF 
      KEQCGEMNW MQFYGQFPE LLKLQLFNL YGV
    CDS sequence
    • >TC012008 RA
      atggactgg tcattatgg aggcctcgc gaagagacc 
      ctggccacg atcaaggaa ctgttacaa gacgctctg 
      atccccgac agcgaggtc caaaaagac gtccagacc 
      aagctgaaa aacctggaa attgtggac gacttcacc 
      tactatttg ttgtacatc gtcggccag gcgcacttc 
      tcggaggaa atccgctcg ttgagcggc attctcctc 
      aagaacaac atcgcggcg gtgtacaac aaattgcca 
      gaggacagt attattaag ataagacag ttgtgtttg 
      atgttgttg cgtgatccg tgtcgcgac gtccgcaca 
      agcatctcc aacgtgatt tacaccttg gccaagtac 
      aacctcaac acgtggccc gaactgatc ccgttcctc 
      gtcaagtcc ttcgagaca cccgacgag tacagcgag 
      gttgcccta acgacgctg ttcagcgtt tgcgaagaa 
      atgatgaac ttggagaag tccgaagag gaaatttgc 
      accatcact aaggaggtg ctcccgaaa ttcgtggat 
      tgtctagtc gacgaaaag tgcgacaag aaacagagc 
      ataatcaag ctgatcaac cagttcttg caagaccac 
      tacaaggtc atgacacaa tcgattgac ctgactagg 
      tatctgcga aacgtgata cagctcgcc gacaccgac 
      gacttggac atgcaaaag tacatttgc cataccttc 
      gttatgtac attgagtat cgcgaggag tgcctcctg 
      ccgcacctg cacgacgtg atcatgtac ctcctggtg 
      aagacgcaa cacgaggac ctggaggtg gccctaggt 
      gcgtgcgag ttttggtta gcggccacc aaattggcc 
      aactgtaag gagctccta accccttac atcgacaaa 
      ctcgtgccg gttttgctc aaaaacatg aaatattcg 
      tcttttgag ctgatcgcc ctcaaggac acgctaagg 
      gtggaggag caaaatgac gatccttcg aaggagacc 
      ttcccgttt catatgcac gacaagagg atcacccgc 
      gatgacgat actttctcg aacgaggag ggcgacttc 
      ctgggcaat tgcgacgac gtggacgac ttctacgtt 
      ggttggacg ttacggaag tgcagcgcg gcgtcgctg 
      gacgccatc gccgtcaag ttcggggag gatatcctc 
      ccgttgatg attcccttc ctgaatgag ttgctctac 
      caccaggac tttctgatc aaggagagc gccattcta 
      gccttgggc gccatctcc gaagggtgc atcaacggg 
      ctaaagccg cacttgccc tacttggtc cagtacctg 
      atccatagt atgaacgat gatcattcc atggtcagg 
      gtcatcacg tgttggacg ctgagtcgt tatgtctgt 
      tggatcata aactcgcaa ccttcacat tcggtttat 
      ttcattccg gtgatgacg attcttttg aagcatttc 
      atggacgag aataagcgg gtgcaaagg gcggccatt 
      tcggccttt tgtgtgttt caagaggag gcgcagatg 
      cagttgatt ccctacatt gatttgatt ctggaaggg 
      tttcaatta gggttccag aagtttaat taccgcagt 
      ctttatttg ttgtatgat gctataaac gtgttggct 
      ttatcggtc gggagcgag ttgagcaaa ccggaatat 
      atcgaaaaa ctgatgccg cctctaata caaaagctg 
      aacgaatat aataactat tcggacgat cagttcatt 
      gcggttctt gaatgtttg gcaaacata ataccgtca 
      ttggatgcc ggtttcttg ccttacagc gaagtgctt 
      tattcgcaa tgtatggag atcatcatg gatacattt 
      atggcagat gctaatttc caggagaat ttaaacggg 
      tttgatccg cccgataaa gaaccgatg catgtcgct 
      ttggacgtc ctgtacagc atggcagtc ggtttgaaa 
      tcgtacttt tacaaatat gtcgctaat agtaacctg 
      gtctgtctc ctgtatact acgatgcaa gactgttcg 
      tttttaata cgacagtcg tccatcgct ctgtacggc 
      gagttggtc ctgatctgc tacccgtac ttgtcatca 
      agcgtcgat gactacatt aaactgata atcaagaac 
      ctggacgag tgttacgaa ggcgtgtgc aaaaacgcg 
      gcttgggtc attgggaaa ctgacaaca gttatgggt 
      gcggaaatc cgcccctac gttccggaa atcctcgcc 
      gctttcatc aacatttca agaaacccg actgtatct 
      cgcgcgatg catcagact gtttcagtc tcgctctgt 
      acgctttgc tgcgtttgt cccgacatt gccctgacc 
      gacgcggat acagttttg aaaaattgc tgcttgtcg 
      ctgcggagt ttgaaggat tcggacgag aaggatttg 
      gcgtttcgg gggttgtgc caggtggtg gtgcggcac 
      ccggatttc tgtcagaac aactttatg tacttttgc 
      gacgcggtg gcgtcgtgg aactccgtc aagcccgat 
      ttgaaagag atgattaag aatattctg gtgtcgttt 
      aaggaacag tgcggggag atgaattgg atgcagttc 
      tacggccag tttcccgaa ttgttgaaa ttgcagttg 
      tttaatctg tatggggtt tga
    mRNA sequence
    • >TC012008 RA
      cgattatca gctggcatc ggtttttgt gaatcaccg 
      gctgtcaaa tcggcgttt ttgacgggt gagaacgat 
      ggactggtc attatggag gcctcgcga agagaccct 
      ggccacgat caaggaact gttacaaga cgctctgat 
      ccccgacag cgaggtcca aaaagacgt ccagaccaa 
      gctgaaaaa cctggaaat tgtggacga cttcaccta 
      ctatttgtt gtacatcgt cggccaggc gcacttctc 
      ggaggaaat ccgctcgtt gagcggcat tctcctcaa 
      gaacaacat cgcggcggt gtacaacaa attgccaga 
      ggacagtat tattaagat aagacagtt gtgtttgat 
      gttgttgcg tgatccgtg tcgcgacgt ccgcacaag 
      catctccaa cgtgattta caccttggc caagtacaa 
      cctcaacac gtggcccga actgatccc gttcctcgt 
      caagtcctt cgagacacc cgacgagta cagcgaggt 
      tgccctaac gacgctgtt cagcgtttg cgaagaaat 
      gatgaactt ggagaagtc cgaagagga aatttgcac 
      catcactaa ggaggtgct cccgaaatt cgtggattg 
      tctagtcga cgaaaagtg cgacaagaa acagagcat 
      aatcaagct gatcaacca gttcttgca agaccacta 
      caaggtcat gacacaatc gattgacct gactaggta 
      tctgcgaaa cgtgataca gctcgccga caccgacga 
      cttggacat gcaaaagta catttgcca taccttcgt 
      tatgtacat tgagtatcg cgaggagtg cctcctgcc 
      gcacctgca cgacgtgat catgtacct cctggtgaa 
      gacgcaaca cgaggacct ggaggtggc cctaggtgc 
      gtgcgagtt ttggttagc ggccaccaa attggccaa 
      ctgtaagga gctcctaac cccttacat cgacaaact 
      cgtgccggt tttgctcaa aaacatgaa atattcgtc 
      ttttgagct gatcgccct caaggacac gctaagggt 
      ggaggagca aaatgacga tccttcgaa ggagacctt 
      cccgtttca tatgcacga caagaggat cacccgcga 
      tgacgatac tttctcgaa cgaggaggg cgacttcct 
      gggcaattg cgacgacgt ggacgactt ctacgttgg 
      ttggacgtt acggaagtg cagcgcggc gtcgctgga 
      cgccatcgc cgtcaagtt cggggagga tatcctccc 
      gttgatgat tcccttcct gaatgagtt gctctacca 
      ccaggactt tctgatcaa ggagagcgc cattctagc 
      cttgggcgc catctccga agggtgcat caacgggct 
      aaagccgca cttgcccta cttggtcca gtacctgat 
      ccatagtat gaacgatga tcattccat ggtcagggt 
      catcacgtg ttggacgct gagtcgtta tgtctgttg 
      gatcataaa ctcgcaacc ttcacattc ggtttattt 
      cattccggt gatgacgat tcttttgaa gcatttcat 
      ggacgagaa taagcgggt gcaaagggc ggccatttc 
      ggccttttg tgtgtttca agaggaggc gcagatgca 
      gttgattcc ctacattga tttgattct ggaagggtt 
      tcaattagg gttccagaa gtttaatta ccgcagtct 
      ttatttgtt gtatgatgc tataaacgt gttggcttt 
      atcggtcgg gagcgagtt gagcaaacc ggaatatat 
      cgaaaaact gatgccgcc tctaataca aaagctgaa 
      cgaatataa taactattc ggacgatca gttcattgc 
      ggttcttga atgtttggc aaacataat accgtcatt 
      ggatgccgg tttcttgcc ttacagcga agtgcttta 
      ttcgcaatg tatggagat catcatgga tacatttat 
      ggcagatgc taatttcca ggagaattt aaacgggtt 
      tgatccgcc cgataaaga accgatgca tgtcgcttt 
      ggacgtcct gtacagcat ggcagtcgg tttgaaatc 
      gtactttta caaatatgt cgctaatag taacctggt 
      ctgtctcct gtatactac gatgcaaga ctgttcgtt 
      tttaatacg acagtcgtc catcgctct gtacggcga 
      gttggtcct gatctgcta cccgtactt gtcatcaag 
      cgtcgatga ctacattaa actgataat caagaacct 
      ggacgagtg ttacgaagg cgtgtgcaa aaacgcggc 
      ttgggtcat tgggaaact gacaacagt tatgggtgc 
      ggaaatccg cccctacgt tccggaaat cctcgccgc 
      tttcatcaa catttcaag aaacccgac tgtatctcg 
      cgcgatgca tcagactgt ttcagtctc gctctgtac 
      gctttgctg cgtttgtcc cgacattgc cctgaccga 
      cgcggatac agttttgaa aaattgctg cttgtcgct 
      gcggagttt gaaggattc ggacgagaa ggatttggc 
      gtttcgggg gttgtgcca ggtggtggt gcggcaccc 
      ggatttctg tcagaacaa ctttatgta cttttgcga 
      cgcggtggc gtcgtggaa ctccgtcaa gcccgattt 
      gaaagagat gattaagaa tattctggt gtcgtttaa 
      ggaacagtg cggggagat gaattggat gcagttcta 
      cggccagtt tcccgaatt gttgaaatt gcagttgtt 
      taatctgta tggggtttg atcacagtt gggattacg 
      aggggatcg aattttttg ggtgggata gcttgagtg 
      cacctgcct tgagttaaa tcgcgtcat atcgtccgt 
      tgggaggga tcgcggcta tcataacaa tcgagaaaa 
      aattcaata tcacagtca ggggattca ttgattatc 
      gtggaatat gatttacat caatgcatt ttagaaata 
      catttttta atcgttacg tgtttttct tgtttttcg 
      taataagca tgctacgtt ttgtacatt atgttgaat 
      ggttggttt gcaactgtt acactactt ttcatgctg 
      tattgttca ttgtggtta tcaataaat gcgtaaata 
      agaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY