Status
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Gene information
General information
TC011181 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3149921..3151222
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011181
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09464

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber sometimes latenumber increased - Number of animals showing the phenotype: 2

    vitellogenic egg chamber late sometimesstructure irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    3
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011181 RA
      MSSELDQWI EIVKDCKYL TENDLKKLC SIVCDILLE 
ESNVQPVST PVTVCGDIH GQFYDLEEL FRQGGQVPD 
TNYIFLGDF VDRGYYSLE TFTRLLTLK AAYPNKITL 
LRGNHESRQ ITQVYGFYD ECLTKYGNA NAWKYCCSV 
FDLLTVAAL IDEKVLCVH GGLSPDIKT VDQIRTLER 
NQEIPHKGA FCDLVWSDP EDVDTWASS PRGAGYLFG 
AKVTHEFMQ INALTLICR AHQLVHEGY KYMFDDKLI 
TVWSAPNYC YRCGNIASI LEFQTTDKV EPKLFNAVP 
DNERVIPSR SVTPYFL
    CDS sequence
    • >TC011181 RA
      atgtcttca gagctggat caatggatt gaaatcgtg 
aaagactgc aagtatttg accgaaaac gacttgaag 
aaattgtgc agtatcgta tgtgatatt cttttggag 
gagtcgaac gtgcagccc gtctccact cctgtgacg 
gtctgtggg gacatccac ggccagttt tatgacctg 
gaggagctg ttccggcaa gggggccaa gtccctgac 
accaattac atattttta ggcgatttt gtcgacagg 
gggtattac agtcttgag acttttact cgattgctc 
actttgaag gcggcttat ccgaacaaa attactttg 
ttaaggggg aaccatgag agtcgacag attacgcaa 
gtttacggt ttttacgac gagtgtctg actaaatat 
ggcaatgct aacgcttgg aaatactgc tgttctgtg 
tttgatctg cttactgtc gctgcgtta attgacgag 
aaagtgctt tgtgtacat gggggctta agtccggat 
attaaaacg gttgaccag attcggact ttggagaga 
aatcaggaa attccgcat aaaggtgcc ttctgtgat 
ttagtttgg tcggaccct gaggatgtg gacacctgg 
gcgtcgagt cccaggggg gctgggtac ttgttcggg 
gcgaaggtc actcatgaa ttcatgcaa attaacgcg 
ctcacatta atttgcaga gctcatcag ttagtccac 
gaaggttat aaatacatg tttgatgat aagctgata 
acggtttgg tcggccccc aattattgc taccgttgt 
ggcaacatt gcaagtatt ttggaattc cagaccacg 
gataaagtc gaaccgaaa ttattcaac gccgtaccc 
gacaatgaa cgagttatt ccaagtaga agtgttact 
ccgtatttt ttgtaa
    mRNA sequence
    • >TC011181 RA
      aatttcagt ttagttaat ttttcaatt ttcgtcgaa 
atttaatta attcattcg gtgggttcg tccgtttgt 
acgtctgtg gttttcaaa ttgtcattt gtcacttgc 
aaactgtca catcttttt ggttatctt cactccaat 
ttaccaaat ttcggcctt tttgctatg ttttatggc 
attatttgc cgcaagacg ttgattatt gccgggctt 
tgacgttca attggtgtt ggaaaagtc gctagacca 
acatgtctt cagagctgg atcaatgga ttgaaatcg 
tgaaagact gcaagtatt tgaccgaaa acgacttga 
agaaattgt gcagtatcg tatgtgata ttcttttgg 
aggagtcga acgtgcagc ccgtctcca ctcctgtga 
cggtctgtg gggacatcc acggccagt tttatgacc 
tggaggagc tgttccggc aagggggcc aagtccctg 
acaccaatt acatatttt taggcgatt ttgtcgaca 
gggggtatt acagtcttg agactttta ctcgattgc 
tcactttga aggcggctt atccgaaca aaattactt 
tgttaaggg ggaaccatg agagtcgac agattacgc 
aagtttacg gtttttacg acgagtgtc tgactaaat 
atggcaatg ctaacgctt ggaaatact gctgttctg 
tgtttgatc tgcttactg tcgctgcgt taattgacg 
agaaagtgc tttgtgtac atgggggct taagtccgg 
atattaaaa cggttgacc agattcgga ctttggaga 
gaaatcagg aaattccgc ataaaggtg ccttctgtg 
atttagttt ggtcggacc ctgaggatg tggacacct 
gggcgtcga gtcccaggg gggctgggt acttgttcg 
gggcgaagg tcactcatg aattcatgc aaattaacg 
cgctcacat taatttgca gagctcatc agttagtcc 
acgaaggtt ataaataca tgtttgatg ataagctga 
taacggttt ggtcggccc ccaattatt gctaccgtt 
gtggcaaca ttgcaagta ttttggaat tccagacca 
cggataaag tcgaaccga aattattca acgccgtac 
ccgacaatg aacgagtta ttccaagta gaagtgtta 
ctccgtatt ttttgtaat tttatgacg ctagtatca 
ataaacaat tttcaaatt t

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY