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Gene information
General information
TC012415 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7001652..7004098
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012415
    iBeetle screen iB_09461

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012415 RA
      MSRLGPEIH PLLTEDVVK ALHGRKVWT VGDFVKVDT 
      QQIIKIARL NFREVRAVK NYLLKKFSA TPVNGFDFY 
      KNVLKNTAI IPTGIKGVD QLLNGGLFT GNIYELCGP 
      PASGKTHFV LTLIKNVIL NMDQNVHIF DTKNDFSAV 
      KMKQMLKNC DEDRRTKSL GKIIVNRCY TRYDLINSL 
      YEIKNDLEN NMKLRLIIV DSLPGVILN SNDHLTNNL 
      YLNHIANIM RYIATEHHV AFLVTNLIT TWTDGGFKT 
      QQETSETIT CGKYWSSVP NTRLRIEKM ENSGGCKLS 
      VLRSDTLNQ KVNSCADIV PTEDGFL
    CDS sequence
    • >TC012415 RA
      atgagtcgt ttagggccc gagatccac cccttgctc 
      accgaggac gtcgtcaag gcgctgcac gggaggaaa 
      gtgtggacg gtgggggac ttcgtcaag gtggacacg 
      cagcagatt atcaaaatc gcacgactc aatttcaga 
      gaagtgcga gcggtaaaa aactatctc ctgaaaaag 
      ttttcagcc acacctgtg aacggattc gatttctat 
      aaaaacgta ctcaaaaat acggccatt atccccaca 
      ggaattaaa ggcgtggac cagttgcta aatggagga 
      ttatttact ggaaatatt tacgagtta tgtgggccc 
      ccagcttcc ggcaaaacc catttcgtt ttaacttta 
      atcaaaaat gtgatcttg aatatggac caaaatgtt 
      cacattttc gacacgaaa aacgacttc tcggctgta 
      aaaatgaag caaatgttg aagaactgt gatgaggac 
      cgacgaact aaaagtttg ggcaagata atagtaaac 
      cgctgttac acaagatac gatttaatc aatagttta 
      tacgaaatt aaaaacgac ttggaaaac aatatgaaa 
      ctacggttg attatcgtc gattctctt cccggcgtg 
      atcttaaat tcaaacgac cacctaaca aacaattta 
      tatctcaat catatcgca aacataatg agatatatt 
      gcaactgag caccatgtc gcattttta gtcacgaat 
      ttaataaca acatggact gacggtggc ttcaagacc 
      cagcaagaa acttccgag actattacg tgtgggaaa 
      tactggtcc agtgtcccc aacactcgc ctcagaatt 
      gaaaaaatg gaaaattca ggcgggtgc aagttgtct 
      gtgttaagg agtgacact ttgaaccaa aaagtgaac 
      agttgtgcg gacattgtg cccactgaa gatggattt 
      ttatga
    mRNA sequence
    • >TC012415 RA
      ggcgcgcgg ccgctggtg cgcatgcgc cgcgccgtt 
      tttcaaacg gggtttcgc tgtttttcc acttttgca 
      ggtagatga gtcgtttag ggcccgaga tccacccct 
      tgctcaccg aggacgtcg tcaaggcgc tgcacggga 
      ggaaagtgt ggacggtgg gggacttcg tcaaggtgg 
      acacgcagc agattatca aaatcgcac gactcaatt 
      tcagagaag tgcgagcgg taaaaaact atctcctga 
      aaaagtttt cagccacac ctgtgaacg gattcgatt 
      tctataaaa acgtactca aaaatacgg ccattatcc 
      ccacaggaa ttaaaggcg tggaccagt tgctaaatg 
      gaggattat ttactggaa atatttacg agttatgtg 
      ggcccccag cttccggca aaacccatt tcgttttaa 
      ctttaatca aaaatgtga tcttgaata tggaccaaa 
      atgttcaca ttttcgaca cgaaaaacg acttctcgg 
      ctgtaaaaa tgaagcaaa tgttgaaga actgtgatg 
      aggaccgac gaactaaaa gtttgggca agataatag 
      taaaccgct gttacacaa gatacgatt taatcaata 
      gtttatacg aaattaaaa acgacttgg aaaacaata 
      tgaaactac ggttgatta tcgtcgatt ctcttcccg 
      gcgtgatct taaattcaa acgaccacc taacaaaca 
      atttatatc tcaatcata tcgcaaaca taatgagat 
      atattgcaa ctgagcacc atgtcgcat ttttagtca 
      cgaatttaa taacaacat ggactgacg gtggcttca 
      agacccagc aagaaactt ccgagacta ttacgtgtg 
      ggaaatact ggtccagtg tccccaaca ctcgcctca 
      gaattgaaa aaatggaaa attcaggcg ggtgcaagt 
      tgtctgtgt taaggagtg acactttga accaaaaag 
      tgaacagtt gtgcggaca ttgtgccca ctgaagatg 
      gatttttat gatctagaa atttatttt aatacaact 
      gtcgcgttt tatcatgta gggatcgat tttaattaa 
      atttttaca gcgcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY