Status
Please wait ...
Gene information
General information
TC012377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5851567..5855835
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012377
    iBeetle screen iB_09458

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012377 RA
      MVSGGMTCV KYLLFVFNL LFFVSGVVI 
      LAIGAVIYV VYGHYYNFV YDSFQSAPL 
      VLMIVGVII FLVSFFGCC GACKENHCM 
      IITFSVLLL VIFTMEIGT GIAGYVRRN 
      EVEVMLENK LNSTMYEYY SNEKIKETW 
      DIVQHEAKC CGMNGYEDW DKVNHNGSL 
      PHTCCPDTP DDGSCTQKS TNIYKDSCF 
      VILKEVFTK YGSIIGGVG IGIAVCQLM 
      GVIFACCLA RSIRQGYAT V
    CDS sequence
    • >TC012377 RA
      atggtgtct ggaggaatg acgtgtgtt aaatatttg 
      ctgtttgtg ttcaacctc ttgtttttc gtctcaggt 
      gttgtgatt ttggcgatt ggggctgtt atttacgtg 
      gtgtatggg cactattac aatttcgtt tatgacagc 
      ttccagtcg gcgcccctt gtgttgatg atcgtgggg 
      gttatcatt ttcctcgtt tcgttcttc ggctgctgt 
      ggggcgtgc aaggagaac cactgtatg atcatcacg 
      ttctccgtg ttgttgcta gtgatattt acgatggag 
      ataggcact ggcattgca ggctatgtt aggaggaac 
      gaagttgag gtcatgttg gagaacaaa ctcaactcg 
      acaatgtac gaatattac agcaatgag aaaattaaa 
      gaaacgtgg gacattgtc caacatgag gccaaatgt 
      tgcgggatg aatggctac gaagattgg gataaggtg 
      aaccacaat ggcagcctc ccgcacacc tgctgtccc 
      gataccccc gacgatggt tcctgtact caaaaatcg 
      accaacatt tacaaagac tcttgtttc gtaatactc 
      aaggaagtt ttcaccaag tacggttca attattggc 
      ggtgtgggg attggaatc gccgtttgt cagctcatg 
      ggggttatt ttcgcctgt tgcttggcg cgttccatc 
      cgccagggg tatgcaaca gtgtaa
    mRNA sequence
    • >TC012377 RA
      cgtcattac tcaaattag gccccaaat caaaaaatt 
      ggtattttt agagcctgt aaagtttat tttggtgtg 
      ttggcccac ctgccgtca atattttga accggcggt 
      acacaaatc cagtggtgg gggcatctc gcttgtgat 
      tggcggaat ctcgtttca ggcatttca ggtatcacg 
      tttgcattt cttattgtg gttgtcctg aaagaggac 
      gaagcgaag cgaggaaag tgttaatga agtgtttgt 
      tgacaatac cacgtctgt tcaccttac gaagatttt 
      ccaataaat tttatcaaa atggtgtct ggaggaatg 
      acgtgtgtt aaatatttg ctgtttgtg ttcaacctc 
      ttgtttttc gtctcaggt gttgtgatt ttggcgatt 
      ggggctgtt atttacgtg gtgtatggg cactattac 
      aatttcgtt tatgacagc ttccagtcg gcgcccctt 
      gtgttgatg atcgtgggg gttatcatt ttcctcgtt 
      tcgttcttc ggctgctgt ggggcgtgc aaggagaac 
      cactgtatg atcatcacg ttctccgtg ttgttgcta 
      gtgatattt acgatggag ataggcact ggcattgca 
      ggctatgtt aggaggaac gaagttgag gtcatgttg 
      gagaacaaa ctcaactcg acaatgtac gaatattac 
      agcaatgag aaaattaaa gaaacgtgg gacattgtc 
      caacatgag gccaaatgt tgcgggatg aatggctac 
      gaagattgg gataaggtg aaccacaat ggcagcctc 
      ccgcacacc tgctgtccc gataccccc gacgatggt 
      tcctgtact caaaaatcg accaacatt tacaaagac 
      tcttgtttc gtaatactc aaggaagtt ttcaccaag 
      tacggttca attattggc ggtgtgggg attggaatc 
      gccgtttgt cagctcatg ggggttatt ttcgcctgt 
      tgcttggcg cgttccatc cgccagggg tatgcaaca 
      gtgtaagca ttgtaatct cacttaaag tttttatgt 
      ttttattta tcgtattgt tgtattttt gagattagt 
      ttattactc aagtttttt aaataatgg gtctgatgt 
      tctcgaata caatcttga atcactttc acttctgac 
      tgatttatc gcacttttc tcaactttg ctgaaagat 
      actattttt tctacagct tttcgtata ggtaagttt 
      ttaaaatat ggcatctat aatcgagta ttgtttgaa 
      caaattcta ttgtacatt aagaagaaa aataacaaa 
      atgtatttt tctgagcca aatttcgta cgaggggag 
      tcaataaat aactttcta tggattttt gataagaaa 
      aatatttat ttgtatgtc ttatgctct ttgtatgta 
      agatattga actgaattt ttacaatag aggctacta 
      gagagacac ttaagttag cgaaatgtg tatttatct 
      ctgtttgtt gtaaaggtt tggttatcc tttctatat 
      tgtgactgt gacagtata tcgtctaga atctataaa 
      ccacagttg agctctatg tctagttgt acactcagt 
      gataaatac ctattaatt attgatgtt atattatta 
      taattgtaa taaagttga tgaaaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY