Status
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Gene information
General information
TC012374 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5823867..5827845
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012374
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012374 RA
      MGSLASRQH AGVEEVDVV SNHAYKYPP RSGNYFGSH 
      FIMGGERFD TPQPESYLF GENSDLNFL GSRPTPFPY 
      PPPQPNEPT KTLKSLVNI RKESLRFVR APADYSKPA 
      EKNERDIRD IELGKSSTF NIEFTFDCD VRCAITIYY 
      FCTEEFTPS GVTYLPRDP NTTSETFHY KRGANQQFC 
      QMTHVFDPS KYTEEELLY DVDREIIPI AIHCVAEEG 
      AEEMRQSHT TIATAEKLS DGTYVLKAL KQKLFVDGL 
      CYLLQEIYG IENKNNDKQ SGDDETEDN GSECVICMC 
      DVRDTLILP CRHLCLCNS CADSLRYQA NNCPICRAP 
      FRALLQIRA LQKCANPNL AQINPPDGS CDNIPPGYE 
      AVSLIEALN GPCIPRQPP SAMPDLVET PESEHAIQA 
      AQILNRSAD RTPLKSKGS DPTTPEYGV LMTTRVEED 
      TKETPKCPL LEQKISSPR LKHRIHDAV KHVNEKSEK 
      DENGDEDSD AEKLSPLLQ NSENLRNGS LALNMVDVV 
      EPIDDTDTD HEDIASRPA SEKTKTYRS NGEERGDES 
      DYYTPEEPA PTPGPLYES TGATSSVES TPQKLPGTP 
      MSHASARSS GDSYTSGSS TRHLLAVVA HNGLNERAV 
      HI
    CDS sequence
    • >TC012374 RA
      atgggttcg ttggctagc agacaacac gcaggggtc 
      gaggaagtg gacgtggtg tcaaaccac gcgtacaag 
      tacccgccc aggtctggg aactacttc gggagccac 
      tttataatg gggggcgag cgcttcgac accccccag 
      cccgagtct tacctcttc ggcgagaac tccgatttg 
      aatttcctg ggcagtaga ccaacccct tttccctac 
      cccccgccc cagcctaat gagcccaca aaaacgctc 
      aaaagtctg gtgaacatc cggaaagag tcgctgcgg 
      tttgtccgc gcccctgct gattattcc aaaccggcg 
      gaaaagaac gagcgggac atcagggat attgagctg 
      ggcaagagc tcgacgttt aatatcgag ttcacgttc 
      gattgtgac gtcaggtgt gcaataaca atctactat 
      ttttgtacc gaggagttt acaccaagt ggagtcacg 
      tacttgccg cgtgaccct aacacgacc tccgagact 
      tttcattac aaacgcggt gcgaaccaa cagttttgc 
      caaatgact cacgttttt gatccctcg aagtacaca 
      gaagaggag ttgttgtat gatgttgac cgggagatt 
      attccaatt gccattcac tgtgtggcg gaggaaggc 
      gctgaggaa atgagacaa tcacacaca actatcgcc 
      acagccgaa aaattgtcc gacggtact tatgtttta 
      aaagctctc aaacagaaa ttattcgtt gacggtctt 
      tgctacctc ctgcaggaa atatacggc attgagaac 
      aaaaataac gacaagcaa tccggcgac gacgagact 
      gaagataac ggttcggaa tgcgttatt tgcatgtgc 
      gacgttcga gacactctg atcctacct tgccgccac 
      ctgtgtttg tgtaattca tgcgctgac tcgctccgt 
      tatcaagca aataattgc ccgatttgt cgcgccccc 
      ttccgggcg cttttacaa attcgggca ttgcaaaag 
      tgcgccaac ccgaatctg gctcaaatc aatccacca 
      gacggaagt tgcgataat atcccgcca ggttacgag 
      gcagtttcg ctgattgag gccttgaac ggtccgtgt 
      attccccga cagccccca tcggcaatg cccgacttg 
      gtcgagact cccgaaagt gagcatgcc attcaagct 
      gcccagatt ttaaaccgc agtgctgat cgcacgcca 
      ctgaagagc aaggggtcg gaccctacg actcctgag 
      tatggggtt ttgatgacg actcgtgtg gaggaggac 
      actaaggag acccccaaa tgtcccctc cttgagcag 
      aaaatttca agtccgcgt ctgaagcac cgcatccac 
      gatgcagtt aaacacgtt aatgagaag tctgaaaaa 
      gatgaaaat ggtgatgaa gacagtgat gctgaaaaa 
      ctgtctcct ttgttgcaa aacagcgaa aatctgaga 
      aatgggagt ttggcgttg aatatggtg gatgtggtt 
      gagcctatt gatgataca gacacggat catgaagat 
      attgcttca aggccggct tcggaaaaa acaaaaaca 
      tatcggagc aatggtgaa gagcgagga gacgagtct 
      gattattac actcctgaa gagccagcc ccgactcct 
      gggccacta tacgagagt actggtgct actagttct 
      gttgaaagt actccgcaa aaattaccg gggacgccg 
      atgtcgcat gctagtgct agaagtagt ggggatagc 
      tatactagc gggtcaagc actagacat ttgttggct 
      gttgtggct cataatggg ttaaatgaa agagctgta 
      catatttaa
    mRNA sequence
    • >TC012374 RA
      taatttctg attttggaa aaacttggc aatttcaag 
      cccgcagaa tgggttcgt tggctagca gacaacacg 
      caggggtcg aggaagtgg acgtggtgt caaaccacg 
      cgtacaagt acccgccca ggtctggga actacttcg 
      ggagccact ttataatgg ggggcgagc gcttcgaca 
      ccccccagc ccgagtctt acctcttcg gcgagaact 
      ccgatttga atttcctgg gcagtagac caacccctt 
      ttccctacc ccccgcccc agcctaatg agcccacaa 
      aaacgctca aaagtctgg tgaacatcc ggaaagagt 
      cgctgcggt ttgtccgcg cccctgctg attattcca 
      aaccggcgg aaaagaacg agcgggaca tcagggata 
      ttgagctgg gcaagagct cgacgttta atatcgagt 
      tcacgttcg attgtgacg tcaggtgtg caataacaa 
      tctactatt tttgtaccg aggagttta caccaagtg 
      gagtcacgt acttgccgc gtgacccta acacgacct 
      ccgagactt ttcattaca aacgcggtg cgaaccaac 
      agttttgcc aaatgactc acgtttttg atccctcga 
      agtacacag aagaggagt tgttgtatg atgttgacc 
      gggagatta ttccaattg ccattcact gtgtggcgg 
      aggaaggcg ctgaggaaa tgagacaat cacacacaa 
      ctatcgcca cagccgaaa aattgtccg acggtactt 
      atgttttaa aagctctca aacagaaat tattcgttg 
      acggtcttt gctacctcc tgcaggaaa tatacggca 
      ttgagaaca aaaataacg acaagcaat ccggcgacg 
      acgagactg aagataacg gttcggaat gcgttattt 
      gcatgtgcg acgttcgag acactctga tcctacctt 
      gccgccacc tgtgtttgt gtaattcat gcgctgact 
      cgctccgtt atcaagcaa ataattgcc cgatttgtc 
      gcgccccct tccgggcgc ttttacaaa ttcgggcat 
      tgcaaaagt gcgccaacc cgaatctgg ctcaaatca 
      atccaccag acggaagtt gcgataata tcccgccag 
      gttacgagg cagtttcgc tgattgagg ccttgaacg 
      gtccgtgta ttccccgac agcccccat cggcaatgc 
      ccgacttgg tcgagactc ccgaaagtg agcatgcca 
      ttcaagctg cccagattt taaaccgca gtgctgatc 
      gcacgccac tgaagagca aggggtcgg accctacga 
      ctcctgagt atggggttt tgatgacga ctcgtgtgg 
      aggaggaca ctaaggaga cccccaaat gtcccctcc 
      ttgagcaga aaatttcaa gtccgcgtc tgaagcacc 
      gcatccacg atgcagtta aacacgtta atgagaagt 
      ctgaaaaag atgaaaatg gtgatgaag acagtgatg 
      ctgaaaaac tgtctcctt tgttgcaaa acagcgaaa 
      atctgagaa atgggagtt tggcgttga atatggtgg 
      atgtggttg agcctattg atgatacag acacggatc 
      atgaagata ttgcttcaa ggccggctt cggaaaaaa 
      caaaaacat atcggagca atggtgaag agcgaggag 
      acgagtctg attattaca ctcctgaag agccagccc 
      cgactcctg ggccactat acgagagta ctggtgcta 
      ctagttctg ttgaaagta ctccgcaaa aattaccgg 
      ggacgccga tgtcgcatg ctagtgcta gaagtagtg 
      gggatagct atactagcg ggtcaagca ctagacatt 
      tgttggctg ttgtggctc ataatgggt taaatgaaa 
      gagctgtac atatttaat tatgtacat tttaatttt 
      attggtaat tacgggcac acaattatt ttatttgta 
      ttatttgag cacggtttt accagtttg tgacttgac 
      attaggtaa cgagattca tttttttaa ccaaaatac 
      tcatttgtg ataaattga ggcatttaa acgtcccaa 
      atcaatgga ttttaatgc ggaaatatc gaatttttc 
      atttatgta tatattgtg cagttgtac ctacaattt 
      agtcttaat taactctac aggtttgga taatcaagt 
      aatttatat tttaaaggt gatattggt tttgtatat 
      aatttgtta aaatatatg tctatcaaa tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY