Status
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Gene information
General information
TC012343 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5118338..5125110
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012343
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09454

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012343 RA
      MVVKIQMKR NVRILLVGD RGVGKTSLI LSLVSEEFP 
      ENVPSKAEE ITIPADVTP EQVPTNIVD YSAAEQSDE 
      QLNEQIKKA NVICVVYAV DDDDSIDRI SSHWMPLIR 
      QNHPDKPCP VVLVGNKID LVDYSTIDG VFQVMDEFS 
      EIETCIECS AKTLKNISE MFYYAQKAV LHPTPPIYS 
      VEKADLTDA CKKALIRIF KICDIDCDG LLNDIELNN 
      FQSRCFNAP LQPQVLDDV KAVLRKNID DGVSHNCVT 
      LKGFLYLHT LFIQRGRNE TTWTVLRKF GYNDNLNMC 
      KEYLFPNLK VPSGCTTEL SHKGYQFLT HIFERFDKD 
      RDHALSPSE YTELFSTCP TPAWPPDVS AMVPTNEKG 
      WITYQGYMC QWALMTLVD LPRTFEYLA YLGYNIYEN 
      ESQVSAVQV TREKKIDLA KKQSSRNVY QCHVIGPMG 
      AGKSSFCRS FIRSPTEQK SPHLENTGT PTCTVNVVQ 
      VYGQEKIMV LRDINVRNV SDPLLPHEV QCDVACLVY 
      DNNNPKSFE YIARIYIKY FAESKIPVL VVACKSDLE 
      EVKQEYLLQ PLTFCQKYK VLPPQNFSV KGHMRKDVF 
      VKLATMAAF PNLREFGIL PTDTRTWWK AGLSLAAVT 
      AVGLIVAKV LHSSDKVR
    CDS sequence
    • >TC012343 RA
      atggttgtt aagatccag atgaagcgc aacgttcgc 
      atcctccta gtcggggac cgcggcgtc ggcaagacc 
      tcgctcatt ctctcgctg gtcagtgaa gagttcccg 
      gaaaatgtg cccagcaag gcggaggaa attaccatc 
      ccggccgac gtcaccccc gagcaagtg cccacgaat 
      atcgtcgat tactcggcg gctgagcaa agcgacgag 
      cagctgaac gagcaaatt aagaaagcc aacgttatt 
      tgcgtggtt tacgccgtg gacgatgac gacagcatt 
      gacaggatt tcctcgcac tggatgccc ctcatccgg 
      caaaaccac ccagacaag ccctgccct gtggtcctc 
      gtgggcaac aagatcgac ctggtggac tattccacg 
      attgacggc gttttccaa gtcatggac gaattttcc 
      gaaatagaa acatgtata gagtgttca gctaaaact 
      ttgaaaaac atctcggag atgttttac tatgcacag 
      aaggccgtg cttcaccca acgccccct atttactcc 
      gtggaaaag gctgatctg actgacgcg tgcaaaaag 
      gctttaatt cggattttt aaaatctgc gatattgat 
      tgcgacgga cttttgaat gatattgaa ctgaataat 
      ttccaaagt cggtgtttt aatgcccct ttacagccc 
      caagtcctt gatgacgtc aaagccgtc ttgaggaaa 
      aacatcgac gatggggtt agtcataat tgtgtcacg 
      ttgaagggc ttcttgtat ttgcacacg ctgttcata 
      caaaggggg cgaaatgag accacctgg acagtttta 
      agaaagttt ggttacaac gacaacttg aatatgtgt 
      aaagaatat ctgtttcca aatttgaag gtaccgtct 
      ggctgtacc acggaactg tcccataaa gggtaccaa 
      ttcctgacc cacatattt gaaaggttt gataaagac 
      cgcgaccat gcgttatca ccctctgaa tacactgaa 
      cttttctcg acttgtccg acccctgcg tggcccccc 
      gatgtgagt gcaatggtc ccaaccaac gaaaagggt 
      tggatcaca taccagggt tatatgtgt caatgggcg 
      ctcatgact ttggtagac ctgccccga accttcgag 
      tatttagct tacttaggt tacaacatt tacgagaat 
      gaaagtcag gtatcagct gtacaagta acgagagaa 
      aagaaaatt gatttggcg aaaaaacag tcgagtcga 
      aatgtgtat cagtgtcac gtgattgga ccaatgggg 
      gcggggaag tccagtttc tgtcggagt tttattaga 
      tcgcctact gaacaaaaa agtccccac ttggaaaat 
      acaggaacg cccacttgt acagttaat gtcgtgcag 
      gtctacggt caggaaaaa ataatggtg ctacgtgac 
      attaatgtg aggaacgtg tctgacccc ttattgcct 
      catgaggtt cagtgtgat gttgcttgt ttggtctat 
      gacaacaat aatccaaag tcgtttgaa tatatcgca 
      cgtatttat attaaatat tttgccgag agtaaaata 
      ccggttttg gtagtcgca tgtaaaagc gatttggag 
      gaagtaaag caggagtat ttattacaa ccgcttaca 
      ttttgtcaa aaatacaaa gtactgcca cctcagaat 
      ttttcagtt aaagggcac atgagaaag gatgtgttt 
      gtcaagttg gcaacaatg gctgctttc ccgaatttg 
      agagaattc ggaatactt cctacggat acgaggacg 
      tggtggaag gcgggattg agtctagcg gcagttacg 
      gcagtcggt ctaattgtc gcgaaagtt ttgcatagt 
      tctgataaa gtcagatag
    mRNA sequence
    • >TC012343 RA
      cggtttggg tgtgagtga caaagtgtc ccaatccgg 
      ccctggtaa aaattctaa gatggttgt taagatcca 
      gatgaagcg caacgttcg catcctcct agtcgggga 
      ccgcggcgt cggcaagac ctcgctcat tctctcgct 
      ggtcagtga agagttccc ggaaaatgt gcccagcaa 
      ggcggagga aattaccat cccggccga cgtcacccc 
      cgagcaagt gcccacgaa tatcgtcga ttactcggc 
      ggctgagca aagcgacga gcagctgaa cgagcaaat 
      taagaaagc caacgttat ttgcgtggt ttacgccgt 
      ggacgatga cgacagcat tgacaggat ttcctcgca 
      ctggatgcc cctcatccg gcaaaacca cccagacaa 
      gccctgccc tgtggtcct cgtgggcaa caagatcga 
      cctggtgga ctattccac gattgacgg cgttttcca 
      agtcatgga cgaattttc cgaaataga aacatgtat 
      agagtgttc agctaaaac tttgaaaaa catctcgga 
      gatgtttta ctatgcaca gaaggccgt gcttcaccc 
      aacgccccc tatttactc cgtggaaaa ggctgatct 
      gactgacgc gtgcaaaaa ggctttaat tcggatttt 
      taaaatctg cgatattga ttgcgacgg acttttgaa 
      tgatattga actgaataa tttccaaag tcggtgttt 
      taatgcccc tttacagcc ccaagtcct tgatgacgt 
      caaagccgt cttgaggaa aaacatcga cgatggggt 
      tagtcataa ttgtgtcac gttgaaggg cttcttgta 
      tttgcacac gctgttcat acaaagggg gcgaaatga 
      gaccacctg gacagtttt aagaaagtt tggttacaa 
      cgacaactt gaatatgtg taaagaata tctgtttcc 
      aaatttgaa ggtaccgtc tggctgtac cacggaact 
      gtcccataa agggtacca attcctgac ccacatatt 
      tgaaaggtt tgataaaga ccgcgacca tgcgttatc 
      accctctga atacactga acttttctc gacttgtcc 
      gacccctgc gtggccccc cgatgtgag tgcaatggt 
      cccaaccaa cgaaaaggg ttggatcac ataccaggg 
      ttatatgtg tcaatgggc gctcatgac tttggtaga 
      cctgccccg aaccttcga gtatttagc ttacttagg 
      ttacaacat ttacgagaa tgaaagtca ggtatcagc 
      tgtacaagt aacgagaga aaagaaaat tgatttggc 
      gaaaaaaca gtcgagtcg aaatgtgta tcagtgtca 
      cgtgattgg accaatggg ggcggggaa gtccagttt 
      ctgtcggag ttttattag atcgcctac tgaacaaaa 
      aagtcccca cttggaaaa tacaggaac gcccacttg 
      tacagttaa tgtcgtgca ggtctacgg tcaggaaaa 
      aataatggt gctacgtga cattaatgt gaggaacgt 
      gtctgaccc cttattgcc tcatgaggt tcagtgtga 
      tgttgcttg tttggtcta tgacaacaa taatccaaa 
      gtcgtttga atatatcgc acgtattta tattaaata 
      ttttgccga gagtaaaat accggtttt ggtagtcgc 
      atgtaaaag cgatttgga ggaagtaaa gcaggagta 
      tttattaca accgcttac attttgtca aaaatacaa 
      agtactgcc acctcagaa tttttcagt taaagggca 
      catgagaaa ggatgtgtt tgtcaagtt ggcaacaat 
      ggctgcttt cccgaattt gagagaatt cggaatact 
      tcctacgga tacgaggac gtggtggaa ggcgggatt 
      gagtctagc ggcagttac ggcagtcgg tctaattgt 
      cgcgaaagt tttgcatag ttctgataa agtcagata 
      gtttgaaag gcacgaata ttttcttgt accaaataa 
      aggcggaaa tgatcagtg aagcatttt gtttatttt 
      agttttgtg cggatgcaa gaaacaaat tagttacaa 
      tagtgtgcg taatcattt acaagtaca agtttgtat 
      aaattgttt gtgcaattt ttctcaagt caaaagttc 
      gtttgtgat tcagtattg atcttagta aatccaaga 
      ctgtaaata ataaaaata gagtgtatt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY