Status
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Gene information
General information
TC001758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7926182..7928918
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001758
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09451

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001758 RA
      MEEAPEIKQ TWQQKLLFY TTAFFVLLG TFSLFSFLF 
      LVPFVIDPA FTTIFMEFD ETPVLCMTV RVDRRLGVS 
      NCSWTSCRE GCTKDIYDC TQILVHYKK VPPDYNLTA 
      PPDIRTPRE ERSIPEEYD YEKTFSEEE EEETGLVDD 
      EEDSEWYYR EARLLPNVK GCGYPPMLN CSIFLKLYK 
      GIGTNYTCY YSRVDPSMV ISHLDMWQV YMNLVYAMA 
      IPIPSFILS VIYLAIAYF KIYNDDEET APLEKNAEA 
      MDVEGESGP DATPVQPAS GGITPASEA FREDLASFG 
      HHFKVAMVD EMSRDSFTE GVPYSCSIG GNLGKTMTT 
      SISTTGGPI ADI
    CDS sequence
    • >TC001758 RA
      atggaggag gcgccagag atcaagcaa acttggcaa 
      caaaagtta ctcttctac actaccgca ttcttcgtg 
      ttgcttggc acttttagc ttgttttca tttctattc 
      cttgtgcct ttcgtcatt gatccggct tttacgact 
      atttttatg gagtttgat gagactccc gtgttatgt 
      atgacagta agagtggat agacggtta ggtgtctct 
      aattgtagt tggacttcg tgtcgtgaa ggatgcacc 
      aaagatatt tatgactgc acgcaaatc cttgttcat 
      tataagaaa gtacctccc gattacaac ctgacagca 
      cctcccgat atccgaacg cccagagaa gaacgaagc 
      ataccggaa gagtacgac tacgagaag accttctcc 
      gaagaggag gaggaagag acggggctt gtggatgat 
      gaagaagac tccgagtgg tactaccgt gaagcgaga 
      ttactcccg aacgtcaag ggttgtggc tatcctccg 
      atgctcaat tgctccata tttctaaag ttatacaaa 
      ggcataggt actaattac acgtgctat tacagtcgc 
      gtagatcct agtatggtg atcagccac ttggatatg 
      tggcaggtc tatatgaac ttggtctat gctatggca 
      attccaatt ccctccttt atcttgtcc gtaatatat 
      ttggcgatt gcttatttt aaaatatac aatgacgac 
      gaagagact gccccactg gaaaaaaat gctgaagct 
      atggatgta gaaggggaa agtggacca gatgctact 
      cctgtccaa cccgcaagt ggcggaata accccggct 
      agtgaagcg ttccgagag gatctcgcc agttttgga 
      catcacttc aaagtggcg atggtcgac gaaatgagc 
      cgtgatagc tttaccgaa ggcgtgcca tactcgtgt 
      tctatagga gggaatttg ggaaaaaca atgactaca 
      agcatctcg accacagga ggtcccata gctgatatt 
      taa
    mRNA sequence
    • >TC001758 RA
      gcagaagtt gtaaaaaca gctcgaaac cggcgaaat 
      taagcacga agggagtaa agcaacgtg aagggatgt 
      gtttgtgaa ccgttggag tgaaaatac ttacgtgga 
      agggcggtt tcgggtcgg aagtggcac ctcacatgg 
      aggacggcg tgctagtgg tgtttaggt cgtctgacg 
      gggaccggc gccaaaatc gatactgta aatacggac 
      tgcagtgtt cagagcagc agcacttcg tgcatgtgc 
      tccaagtgg ccaagacga gtcaccacg agcaagtgt 
      taaataatg tagactgaa atcgtcgag agtgtttaa 
      tcggataat gtgcaatgt ctgctatgt agtcgaaat 
      caaaaaacg ttggggttg agcggttcc cggttttgc 
      tgtgagctg gccgcagcc gctcgcgcc tgagtgtgg 
      atgcagatg gtggggtcc agggagtcg ttttatctt 
      gcaccatgg aggaggcgc cagagatca agcaaactt 
      ggcaacaaa agttactct tctacacta ccgcattct 
      tcgtgttgc ttggcactt ttagcttgt tttcatttc 
      tattccttg tgcctttcg tcattgatc cggctttta 
      cgactattt ttatggagt ttgatgaga ctcccgtgt 
      tatgtatga cagtaagag tggatagac ggttaggtg 
      tctctaatt gtagttgga cttcgtgtc gtgaaggat 
      gcaccaaag atatttatg actgcacgc aaatccttg 
      ttcattata agaaagtac ctcccgatt acaacctga 
      cagcacctc ccgatatcc gaacgccca gagaagaac 
      gaagcatac cggaagagt acgactacg agaagacct 
      tctccgaag aggaggagg aagagacgg ggcttgtgg 
      atgatgaag aagactccg agtggtact accgtgaag 
      cgagattac tcccgaacg tcaagggtt gtggctatc 
      ctccgatgc tcaattgct ccatatttc taaagttat 
      acaaaggca taggtacta attacacgt gctattaca 
      gtcgcgtag atcctagta tggtgatca gccacttgg 
      atatgtggc aggtctata tgaacttgg tctatgcta 
      tggcaattc caattccct cctttatct tgtccgtaa 
      tatatttgg cgattgctt attttaaaa tatacaatg 
      acgacgaag agactgccc cactggaaa aaaatgctg 
      aagctatgg atgtagaag gggaaagtg gaccagatg 
      ctactcctg tccaacccg caagtggcg gaataaccc 
      cggctagtg aagcgttcc gagaggatc tcgccagtt 
      ttggacatc acttcaaag tggcgatgg tcgacgaaa 
      tgagccgtg atagcttta ccgaaggcg tgccatact 
      cgtgttcta taggaggga atttgggaa aaacaatga 
      ctacaagca tctcgacca caggaggtc ccatagctg 
      atatttaat tgctgtcac gtcttgaca tttctcagg 
      cttgtgtgt tttatctta atttgataa ggcttagga 
      taaacatac gaaataaat tttttaaaa tggatgtgg 
      ctgtctctc cgccacaat caacgtatg tttacgtgg 
      cttcaagaa atatttttt aaaagtagt gttttaggc 
      cgctacaaa tgaatatga ataacagct tcaattatc 
      caaaggagc gaatctgat ttcatatta cgagcaaac 
      gtgtataaa ttatcaaaa aagacttaa tggcaacat 
      caaccgggt ctcatgtaa ttgcacatt caacgaaat 
      tttaccaaa ttttatttc taagtggtt aaatttcta 
      gttgtgtaa tcacgtaat tcaattcca attcgttaa 
      cgaaattta aagcaatac tgtgcattc gtttgtgga 
      gtttcagtg aaattggct cctctggtt gtgctacag 
      cgcatttat ctgttatca atctcatta catccatag 
      tttaacacg tccgtgcgt ataccacgt cattttgat 
      tgcattttt ttcctcgat attactgta aatatatag 
      attgtcttt aatattgtt attattaat cacattgtt 
      aaaatttat aagaaaatg ttttattga tttgtatat 
      ttcatgtac agttcgact tatgaaatt ttacctaat 
      cgtgcagtg aaaacgtgt ttattgaac attgttcaa 
      gattttgcc ttcttattc ttgtaatac gcatttgta 
      atctaatat ttttaatac acataacat agatgatac 
      aatcaagtc aactcttct atatgttta caatgcatg 
      ttttcagaa taaggcaat tgtcgcgtg aactttatc 
      aaataattc aatcagatc gattctttt tcttttatt 
      agaaaggga ccaaggcat attgcacgg ggattgtcg 
      caagcatcg acatattga actattttc gagcacatg 
      cagttgtca acatataca ttacttgtg aacttgtgt 
      tacaactat taccctata taattttag gcgagtgtc 
      aatatcggc aatgttctt caccactct atagagtcg 
      atagcttga atcattatg actcactgc tgatatatc 
      aatttgttt taccttgtt accaccgat ttatttaac 
      tcatttgtg atacttaac actgctcat ctaggaata 
      aaatagatt tcagtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY