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Gene information
General information
TC011183 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3143031..3144182
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011183
    iBeetle screen iB_09445

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011183 RA
      MPKSKRDKK ISLTKTDKK GLALKQKIV 
      EDVRNCVEK FSSIYVFTY RNMRNELMK 
      DVREQWKPS RFFFGKNKV IGVGLGRNK 
      EEEVADDLH KLSRCLKGQ CALLFTDSP 
      KDEVIEWFE SYSCEDFAR SGCVVDKTV 
      TLPEGALKQ FPHSMEPYL RQLGMPTKL 
      ERGVVTLIK EFEVCKEGG VLTPEQAKI 
      LEFLGHRLA VFKLDLRAA WIKGSGFER 
      LDGKSEDND ENMNEDEDN
    CDS sequence
    • >TC011183 RA
      atgcctaaa tcaaaacga gacaagaaa atttcttta 
      accaagact gataaaaaa gggctcgcc ctcaagcag 
      aaaatcgtc gaagatgtg cgaaattgc gtcgagaag 
      ttctccagc atttacgtc ttcacttac agaaacatg 
      cggaatgaa ttaatgaaa gatgttagg gaacagtgg 
      aagcctagt aggtttttc tttggaaaa aataaagtt 
      attggtgtg ggtttgggc cgaaacaag gaggaagaa 
      gtggcggat gatttacac aaactttca cgttgtttg 
      aaaggccaa tgtgccctt ctttttact gactctcca 
      aaagacgaa gttattgag tggtttgag agttacagt 
      tgtgaggat tttgcgaga tctgggtgc gtcgttgac 
      aaaactgta actttgccc gagggcgcc cttaagcaa 
      tttccacac tcaatggaa ccgtatttg agacagtta 
      gggatgccg actaaacta gaaagagga gttgtaaca 
      ttaataaaa gagtttgaa gtgtgcaaa gaggggggc 
      gttttgacg ccagaacag gcaaaaatt ttagaattt 
      ttaggacac agattggcc gtttttaaa ctcgacttg 
      agagcggcc tggatcaag ggctcagga ttcgaaaga 
      ctggacggg aagagcgaa gataatgat gaaaacatg 
      aatgaagat gaagataat taa
    mRNA sequence
    • >TC011183 RA
      ccgtgctaa tttttccaa tttttaaat taattttaa 
      gtggaatag agccatcga acatttcag caaaaaaac 
      acccttatt tcaatttgt ttcaatgaa taacactat 
      taattatca gttttttac aattcgtga tttcgggaa 
      cgcttgagc gaaatctgg caaagccga aactcaact 
      agacacacg tgttttggt tttggttat aaaattgtt 
      ttaaataaa ttaggcaca attatttac gtgtagcag 
      agatgccta aatcaaaac gagacaaga aaatttctt 
      taaccaaga ctgataaaa aagggctcg ccctcaagc 
      agaaaatcg tcgaagatg tgcgaaatt gcgtcgaga 
      agttctcca gcatttacg tcttcactt acagaaaca 
      tgcggaatg aattaatga aagatgtta gggaacagt 
      ggaagccta gtaggtttt tctttggaa aaaataaag 
      ttattggtg tgggtttgg gccgaaaca aggaggaag 
      aagtggcgg atgatttac acaaacttt cacgttgtt 
      tgaaaggcc aatgtgccc ttcttttta ctgactctc 
      caaaagacg aagttattg agtggtttg agagttaca 
      gttgtgagg attttgcga gatctgggt gcgtcgttg 
      acaaaactg taactttgc ccgagggcg cccttaagc 
      aatttccac actcaatgg aaccgtatt tgagacagt 
      tagggatgc cgactaaac tagaaagag gagttgtaa 
      cattaataa aagagtttg aagtgtgca aagaggggg 
      gcgttttga cgccagaac aggcaaaaa ttttagaat 
      ttttaggac acagattgg ccgttttta aactcgact 
      tgagagcgg cctggatca agggctcag gattcgaaa 
      gactggacg ggaagagcg aagataatg atgaaaaca 
      tgaatgaag atgaagata attaattgt tattttatt 
      actaattaa taaactcat aatataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY