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Gene information
General information
TC012179 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3890273..3892700
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012179
    iBeetle screen iB_09442

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012179 RA
      MFDKSEMDQ VILATGGYD HTIKLWHTH SGICYRTMQ 
      HAESQVNAL EITPDKQLL AAASYQHIY MYDLNSNNS 
      NPIVNYDGI TKNVTDVGF EVDGRWMYS GGEDCRVRL 
      WDLKSKGTQ CPKIFEVQA PVTCVCLHP NQVELFVGD 
      QSGIIYRWD LKTDHNEQL IPESDAMIL DIAIDPLGT 
      HMAAVNNKG RCYIWSLKS GQIDEPTKM NPKHKFDAH 
      KRHALKCEF SPDSRYLVT TSADQTAKI WNTSDFSLR 
      QELKQENQR WVWDAAFSS DSQYVFTAS SDYTAKIWN 
      IEKGTMEKE YAGHQKAVT ALAFRDCAV
    CDS sequence
    • >TC012179 RA
      atgtttgat aagtctgag atggaccaa gtgatctta 
      gcgaccggg ggctacgac cacaccatc aaattatgg 
      cacacacat tccggtatt tgttacagg acaatgcaa 
      catgccgaa tctcaagtc aacgctctg gaaataacc 
      cccgataag caactgcta gcagccgcg agttaccaa 
      cacatttac atgtacgac ttaaactca aataactcg 
      aaccccatt gtaaactac gacggaatc actaaaaac 
      gtgacggat gtgggtttc gaggtcgac ggtaggtgg 
      atgtactcc gggggtgaa gactgcagg gtgcgtctg 
      tgggacttg aaatcgaaa ggcacccag tgtcccaaa 
      attttcgaa gtccaggcc cctgtgacc tgtgtttgc 
      ctgcacccc aaccaggtg gagttgttc gtaggcgac 
      cagagcggg attatatac aggtgggac ttgaaaacc 
      gaccacaat gaacaacta attcctgaa agcgacgct 
      atgattctg gacattgcg attgaccct ctggggacg 
      cacatggcg gctgtgaac aacaaaggc cgctgttac 
      atctggagc ctcaagtcg gggcagatc gacgaaccg 
      accaaaatg aaccccaaa cacaagttt gacgctcat 
      aaaagacac gcactgaag tgcgaattt agcccagat 
      tcgaggtat ttagtaacc acatcagcg gaccaaacc 
      gccaaaatt tggaacact agtgatttt agtttgaga 
      caggaactg aagcaggag aatcagaga tgggtgtgg 
      gacgctgcc ttcagctcg gactcgcaa tacgttttc 
      acagcttcc tctgactat actgcaaaa atatggaac 
      atagagaag ggtactatg gaaaaggag tacgcaggg 
      caccaaaag gcggtcaca gccttggca tttagggat 
      tgtgcagtg tga
    mRNA sequence
    • >TC012179 RA
      cgatgtagg aaaattata aattcgaat ttagttttt 
      gtgatgatt ttttatggc aacatctgg atcctctga 
      tccattttt ctcctcttt cccatactt caaaacgtc 
      aaagtgaca agaaaccca acctcacat tttcgcaca 
      aattgtgtt tatttcctt caaactaag aaaatacgt 
      gattctgat gtgttaaac tgacgcact taatcatgt 
      ttgataagt ctgagatgg accaagtga tcttagcga 
      ccgggggct acgaccaca ccatcaaat tatggcaca 
      cacattccg gtatttgtt acaggacaa tgcaacatg 
      ccgaatctc aagtcaacg ctctggaaa taacccccg 
      ataagcaac tgctagcag ccgcgagtt accaacaca 
      tttacatgt acgacttaa actcaaata actcgaacc 
      ccattgtaa actacgacg gaatcacta aaaacgtga 
      cggatgtgg gtttcgagg tcgacggta ggtggatgt 
      actccgggg gtgaagact gcagggtgc gtctgtggg 
      acttgaaat cgaaaggca cccagtgtc ccaaaattt 
      tcgaagtcc aggcccctg tgacctgtg tttgcctgc 
      accccaacc aggtggagt tgttcgtag gcgaccaga 
      gcgggatta tatacaggt gggacttga aaaccgacc 
      acaatgaac aactaattc ctgaaagcg acgctatga 
      ttctggaca ttgcgattg accctctgg ggacgcaca 
      tggcggctg tgaacaaca aaggccgct gttacatct 
      ggagcctca agtcggggc agatcgacg aaccgacca 
      aaatgaacc ccaaacaca agtttgacg ctcataaaa 
      gacacgcac tgaagtgcg aatttagcc cagattcga 
      ggtatttag taaccacat cagcggacc aaaccgcca 
      aaatttgga acactagtg attttagtt tgagacagg 
      aactgaagc aggagaatc agagatggg tgtgggacg 
      ctgccttca gctcggact cgcaatacg ttttcacag 
      cttcctctg actatactg caaaaatat ggaacatag 
      agaagggta ctatggaaa aggagtacg cagggcacc 
      aaaaggcgg tcacagcct tggcattta gggattgtg 
      cagtgtgat ttcttgtgt aaattgtta cttgagtta 
      ttaaaacaa caaaatgag t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY