Status
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Gene information
General information
TC012291 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3824493..3827199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012291
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09441

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012291 RA
      MIMQKKQQT GLRHMFAGT GPQILASVA ATISAMNDG 
      MHYGWSAPV IPILQADNT PIQISKVDE SWLEAMYLV 
      GGIAGLPVT IFLVNKIGR KNSTMVSSV TSLISWILI 
      ALASNVTLL YVARFLSGL AGDMAFVAT PMYVAEIAD 
      QKIRGLLSC LIYLMMLFG ILLIYAIAP FTPFYVPSV 
      VGGALLVTQ LASFTFMPK SPYYLITKN KMDKARKAL 
      NRLRTTKDN EAELEEISR AIERQRKEK GRPQDLLLV 
      NSNRKALIV ITMLNGAQH FTGISVMLM NMHTILTKA 
      GSVYLTSNA TAIIFSSCM LIAATISSF AVDKYGRKF 
      LLISSSILT GICLLVLAV YFNLQYSGY DVRAVSWIP 
      IGCVMAYAA TFKMGLGMV PIVISAEIF SNKVKAMGM 
      TIADAMYLV FGILSIELY KHLSESYGY HVPFYIFAC 
      FSFITTVFV VTIVPETKG KSLEEIQLL LKGQKAVVS 
      HEDKDVTTS LTKV
    CDS sequence
    • >TC012291 RA
      atgatcatg cagaagaag cagcaaacc gggctcagg 
      cacatgttc gccggcacg ggcccccag atcctggcc 
      tcggtcgct gcgacgatc agcgcaatg aacgacggc 
      atgcactac ggctggtcc gcgcccgtc atccccata 
      ctccaggcc gacaacacg cccatccag atatcgaaa 
      gtcgacgag agctggctg gaggccatg tacctggtc 
      ggcggcatc gccggcctc cccgtcacc atcttcctc 
      gtcaacaaa atcggccgc aaaaactcc accatggtg 
      tcgtcggtg acgagtctg atatcgtgg attttaata 
      gccttggca tcgaacgtg acgctgctg tacgttgcc 
      agatttctg tccggcctg gccggggac atggcgttc 
      gtggccacc cctatgtac gtggcggag atcgccgac 
      cagaagatc cgggggctg ctctcatgc ctcatctac 
      ctcatgatg ctgttcggg atcctgctc atctacgcc 
      atcgcccct ttcaccccg ttctacgtg ccgtcggtg 
      gtgggtggg gcgctcttg gtgacccag ctggcctcc 
      ttcaccttc atgcccaag tcgccgtac tacctcatc 
      accaagaac aagatggac aaggcccgc aaggccctg 
      aaccggctg cggacgacc aaggacaac gaagccgag 
      ctggaggag atcagcagg gcgatcgaa cgccagcgg 
      aaggagaaa gggcggccg caggacctg ctcctcgtc 
      aacagcaac cgcaaggcg ctgattgtc ataaccatg 
      ctcaacggc gcccagcac ttcaccggg atcagcgtc 
      atgctgatg aacatgcac acgattctg accaaagcc 
      gggtcggtc tatctcacg tcgaacgcc acggcgatc 
      atcttctcg agctgtatg ctcatagcg gcgacgatt 
      tcctcgttt gccgtggac aagtacgga agaaagttc 
      ctcctaatc tcctccagt attctcacc gggatttgt 
      ctcctggtt ttggctgtc tattttaat ttgcagtac 
      agcgggtac gacgtgcgt gccgtgagc tggatcccc 
      atcggctgt gttatggca tacgctgcc acgtttaaa 
      atgggcttg gggatggta ccgattgtg ataagtgcg 
      gagattttt tcaaataag gttaaggcc atggggatg 
      accatagcc gatgctatg tatctcgtt ttcgggatt 
      ctttcgatt gaattgtac aaacaccta agtgagtct 
      tatgggtac catgtaccc ttttatatt ttcgcgtgt 
      ttttctttc attaccaca gtatttgtt gttactatt 
      gtacctgaa acgaaggga aaatcgctg gaggaaatc 
      cagctgcta cttaaggga caaaaagcc gtcgttagc 
      cacgaagac aaagatgtg acgactagt cttacgaaa 
      gtgtga
    mRNA sequence
    • >TC012291 RA
      cggtacata aaaaatatt tcctaccag tggcggcgc 
      gccagatgt tgtcatcgt ttgaacctt tattatttt 
      caatttcaa acttgtaac gcatttcct attcaaggg 
      aatttccca taacatgtg tcataacat gtagctctt 
      gtttaggag ttttagcag ggaattata tcattttgt 
      gatatggag ttaaaaatg taatgtaga ggccatgac 
      agctgcaaa aaacttgct ttgtaaata tggtattac 
      atagctctt tatcttatt tgtgaactc atgagccgc 
      cactgttga aatccagct gataaaagt ccagtgata 
      agtaattct catttcgca ggaggtaag tttgagtca 
      atataacgg acctaacac acgcaacgt gctcccgac 
      cgaaaattc gcgaaactg tgacgtaac tcatgaaga 
      attccgcgt atttaacat ccacgtcta tttataaat 
      caagatgat gtagcaaaa atggaagaa accattcca 
      tcaacgcac ccagcaatt ttttccacc aagccaatt 
      tgaaatatt gtacctgtt acgtgtcaa caataattt 
      agatatagt ttattatca ttctatgta gcgtttaat 
      gtacaagtg gatactcgt gcgaaacgt gtgctcggt 
      gtttgccag ccccgatct atttccaca ccagtcaat 
      catctccat gatcccgac ttcttacct gtccacgct 
      cgtctcgac ctccactcc acgtcatta gctgtcatt 
      ccatgatca tgcagaaga agcagcaaa ccgggctca 
      ggcacatgt tcgccggca cgggccccc agatcctgg 
      cctcggtcg ctgcgacga tcagcgcaa tgaacgacg 
      gcatgcact acggctggt ccgcgcccg tcatcccca 
      tactccagg ccgacaaca cgcccatcc agatatcga 
      aagtcgacg agagctggc tggaggcca tgtacctgg 
      tcggcggca tcgccggcc tccccgtca ccatcttcc 
      tcgtcaaca aaatcggcc gcaaaaact ccaccatgg 
      tgtcgtcgg tgacgagtc tgatatcgt ggattttaa 
      tagccttgg catcgaacg tgacgctgc tgtacgttg 
      ccagatttc tgtccggcc tggccgggg acatggcgt 
      tcgtggcca cccctatgt acgtggcgg agatcgccg 
      accagaaga tccgggggc tgctctcat gcctcatct 
      acctcatga tgctgttcg ggatcctgc tcatctacg 
      ccatcgccc ctttcaccc cgttctacg tgccgtcgg 
      tggtgggtg gggcgctct tggtgaccc agctggcct 
      ccttcacct tcatgccca agtcgccgt actacctca 
      tcaccaaga acaagatgg acaaggccc gcaaggccc 
      tgaaccggc tgcggacga ccaaggaca acgaagccg 
      agctggagg agatcagca gggcgatcg aacgccagc 
      ggaaggaga aagggcggc cgcaggacc tgctcctcg 
      tcaacagca accgcaagg cgctgattg tcataacca 
      tgctcaacg gcgcccagc acttcaccg ggatcagcg 
      tcatgctga tgaacatgc acacgattc tgaccaaag 
      ccgggtcgg tctatctca cgtcgaacg ccacggcga 
      tcatcttct cgagctgta tgctcatag cggcgacga 
      tttcctcgt ttgccgtgg acaagtacg gaagaaagt 
      tcctcctaa tctcctcca gtattctca ccgggattt 
      gtctcctgg ttttggctg tctatttta atttgcagt 
      acagcgggt acgacgtgc gtgccgtga gctggatcc 
      ccatcggct gtgttatgg catacgctg ccacgttta 
      aaatgggct tggggatgg taccgattg tgataagtg 
      cggagattt tttcaaata aggttaagg ccatgggga 
      tgaccatag ccgatgcta tgtatctcg ttttcggga 
      ttctttcga ttgaattgt acaaacacc taagtgagt 
      cttatgggt accatgtac ccttttata ttttcgcgt 
      gtttttctt tcattacca cagtatttg ttgttacta 
      ttgtacctg aaacgaagg gaaaatcgc tggaggaaa 
      tccagctgc tacttaagg gacaaaaag ccgtcgtta 
      gccacgaag acaaagatg tgacgacta gtcttacga 
      aagtgtgat gtttacaac ttgtagatt ataaagaca 
      aataattat ttttgataa agaatgttc ctttagata 
      tgcagattc gtatctcgt ttgatttgt agtatagat 
      agtcaatta ttttgtgaa tttgtagga aaatataca 
      ttgatatat gtaggcggg aaagtagtt acatggatt 
      attttttga cttagcttt aattgcaat tcttacatg 
      acggagttc ccagtttcc caaaagtat aattaaaat 
      gatttacgt caagtacca gtgttacta tgctaatat 
      gtatttatc ggaccgatg accttatcg aacccgtat 
      acgcttcct agtaactgg ggaatcaac agttggcaa 
      aatgaagaa actgtttta tcttatctg aaaaataaa 
      actctcata ccaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY