| General information | |||
| TC001750 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 7986862..7994207 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001750 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09437 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
mature egg not present - Number of animals showing the phenotype: 4
vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
egg chamber stage sequence irregular - Number of animals showing the phenotype: 4
egg chamber potentially number decreased - Number of animals showing the phenotype: 4
vitellarium potentially size decreased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001750 RA
MACYLNENV YTHTFTPRE YQVELLDSA KKRNTIVCS SASSAKAFI TIKLLQEFS HKMRVPHGK QALFVLDGP NVPIMTSHV KLLTDLTVT SIDKEENPP SLKASNVIV TTAEVCVLL CKKNFVHLD SYALIVIDC LYGGQQSLV REIMARYQA IQAPRPRIL GLTAGLLGS EMQPDRLEA ELQRLEKLL SSSVDTSSE ILTLIRLSC RPRERIVEC FKPIPSPLQ DKIKATITS CQDFLKDHR YDPSEIYDD DLLEEFKQV PDPKEQPLS FFDDFLEIL DDLGPWSAD RAAYGMLIK IEKLKVKVP YERHYLLLC VASSVLVSI RALCELEFQ DYTDKEKVF RFSTPKVLR FLQVLKQFK PTGDKPETC DKLPDLKDP KKGKGKNYK GPRRPYISR AQSDEMLCA LVFVKNRYK AEALFALLC VMSKSDEEY WWVSVSFSV NKIADPVRE PREAESEHK RQEEVLRKY RSHECNIMI ATSALEQGC DLPKCNLVI RFDLPQSFH SYIHSKARA RANEAHFLL LANENEVSD FVENLAEYN EVENTLLKR CYSLEPDKN EELVADASS LQCRPYQPS AEPGANSVS LSNAIALVN RYCAKLPSD TFTRLTPIW HEEKVENGY ICSIRLPIN SPVKKTVTS PPMINTLLA RRAAAFMIC QLLHKAGEL DDNLQPIGK ENFKVNEED WNSSALEES DEENLDPRP GTTKRRQYY YKKVADALL DCHPIIGQP TYFYKIVMK LTCPLPEEQ NTRGRKIYP PEDSPQGFG ILTSKEIPK ISAFPIFTR SGEVSVDLQ LCSQLIVTE NQICKIREF LNYTFTSVL RLQKYLTLF NPDASANSY LIVPTIDGA TTTVDWDFI DLIYANLTV LPEIIPEEV RKSYEFDPE KYRDAVVMP WYRNQDQPQ YFYVAEICS NLNPASDFP GSDYATFEE YYLRKYSIQ IQNKSQHLL DVDHTSARL NFLTPRYVN RKGVALPTS SEATKRAKR EKLEQKQIL VPELCAIHP FSASLWRKA VCLPCILYR INALLLADQ IRRTVALEL NLGKIELDS EFKWPPLNF GWSLADVLK KSKDEEKKK QEKIEPVIE EIPCTEIAK IEDFDQDDD EEEMIEIGT WSNDMAQLN SDQEFPVVR YASPTSWMD LQNTYDDSS FSDSDYSGD ESESEWGGL RIEFTGDNV AEAVDDENK KDDDFELVD YSNVWKVED ESEITQTLR KQFHDACAR NKDHILSSG ILVSKSEQF QKCSDCDNT TTKDSQVAN SYDFDFGKL FIELDQHKA LQIDLAPQD ARNEYDISE TMTFKFDEQ PNLVEHPGP SPNALQIDL APQDARNEY DISETMTFK FDEQPNLVE HPGPSPNVL LQALTMSNA NDGINLERL ETIGDSFLK YAITNYLYS KYENVHEGK LSHLRSKQV SNLNLYRLG RRKGLGEYM IATKFDPHD NWLPPCFYV PKELEEALI DAQFPANCW TVADMAATR DMTLDDICS MVRQRGESL SLSNIIPYN LVTQHSIPD KSIADCVEA LIGAYLIEC GPRGALLFM AWLGIRVLP QLEDGTYGE IELPKSPLS NHLTYPREE LDMLLDGYD QFERHIGYK FRDRSYLLQ ALTHASFSP NTLTDCYQR LEFLGDAVL DYLITRHLY EDTRMHSPG ALTDLRSAL VNNTIFASL AVRNGFHRY FRNLSPSLN EVVEKFVRL QEDSGHTLV DELYLVVET EEVEDVEVP KALGDVFES VAGAIFLDS GMSLDAVWK VYYNMMKSE IEQFSNKVP KSPIRELLE LEPETAKFG KPEKLADGR RVRVTVEVF GKGVFKGIG RNYRIAKCT AAKCALKNL KKRGLIKKI DES
- >TC001750 RA
atggcttgt tatttaaac gaaaatgtt tacacgcac acgtttacc ccacgagag tatcaagtg gagctgctg gacagcgct aagaagcgt aatacaatc gtatgttcg agtgcgagt tcggcgaaa gcgtttata acaataaaa ctgctgcaa gagttctcc cacaagatg cgggtgccc cacggaaag caggcttta ttcgtgctg gacggcccg aacgtcccg ataatgacg tcgcacgtg aagctgctg accgatctg accgttacg agcatagat aaagaggaa aaccccccg tcgttgaag gcaagcaat gtgattgtg accacagcc gaagtctgt gtgcttcta tgtaagaag aacttcgtt catttggac agttacgct ctaatcgtg atagactgc ttatacggg gggcaacag tcgttagtt agggaaatt atggcccgg tatcaggcg attcaagcg cccagacct cgaattttg ggcctgact gccggtctt ttgggctcc gagatgcaa cccgatcgt ctagaagcc gaattacaa cgcttagaa aaattactt agttcctcg gtagacact tctagtgaa attctcacg ctgatacgc ttatcttgt cggccgcgc gaacgcata gtcgagtgc tttaaaccc attccaagc cctttgcag gacaaaatc aaggccact ataacctcc tgtcaggat ttcctcaag gaccaccgc tacgatccg agcgaaatc tacgacgat gacttgctc gaggaattc aagcaagtc ccagacccc aaggaacag cctttgagc ttcttcgac gacttcctc gaaattctg gacgatttg gggccctgg tcggccgac agagccgcc tatggcatg cttatcaag atcgagaag ctcaaagtc aaagttcct tacgaaaga cattattta ttgttatgc gttgcttca tcagtatta gtttcgata agggctctc tgcgaactc gaattccaa gactacaca gataaggaa aaggtgttt cgtttctca acgccgaaa gtactcaga tttctccaa gttctaaag cagtttaaa ccgaccggt gataagccg gaaacttgc gacaaacta ccagacctc aaagacccc aagaaggga aaagggaaa aactacaaa gggccccga aggccgtac atctcgcgc gcgcaaagc gacgagatg ctctgcgca ctcgttttc gtcaaaaat cgctacaag gcggaggcc ctcttcgcc ctactgtgt gtgatgtca aagagcgac gaggagtat tggtgggtg tcggtttcg ttctccgtg aataagatc gcagatccg gtgcgcgag cctcgggag gcggaatcc gagcataaa aggcaggag gaagttttg cggaagtac agaagtcac gaatgcaat ataatgata gcaacgtcg gcgttagaa caaggctgt gatcttcct aaatgtaat ttagtgata cgtttcgat ttacctcag tcgttccat agctacatt catagtaaa gcgcgagcg cgcgcaaac gaagctcat tttcttttg ctagcgaac gagaacgaa gttagcgat tttgttgag aatttagcc gagtacaac gaagttgag aatacgcta ttgaagagg tgttacagt ttggaacct gataagaat gaggagctg gttgctgat gcttcgtct ctgcaatgt agaccgtat cagccgtcg gccgaaccg ggggctaac tcagttagt ctctcgaat gctatagct ttagtcaac cggtattgt gcaaaattg cccagcgat acgttcacc cgcttgacg ccaatttgg catgaggag aaggtcgag aatggttat atttgttcc attcggtta cctatcaat tctccggtg aagaaaacg gtgacaagc cctccaatg attaatact ttactggca cgcagagct gcagctttt atgatttgc caactccta cacaaagcg ggggaatta gacgataat ctgcaacca ataggcaaa gagaatttc aaagtgaac gaagaagac tggaatagc tcggcgctt gaagaatca gatgaagaa aatctagac ccgagaccg ggaactacg aaacggcgg cagtattac tataaaaaa gttgccgat gctctactt gactgtcac ccaattatt ggccaaccg acttacttt tacaaaatc gtaatgaaa ctgacctgc cctttgccc gaagaacaa aacaccagg gggcggaaa atataccct ccggaggac tctcctcaa gggtttgga atactgacc tccaaagaa ataccaaag ataagtgcc tttccaatt ttcacaaga tcgggtgaa gtaagtgtt gatttacaa ttgtgttca cagttaatt gtaacggag aatcaaatt tgcaaaatt agggaattt ttaaattac acgtttact agtgtttta cgtttacaa aagtaccta acccttttc aatcctgac gcttcggcc aattcgtac ttaatcgtg ccaacgata gatggtgcc accaccacc gtcgactgg gacttcatc gacttaatc tacgccaac ttgacggta ctccccgag ataattccg gaagaagtc cgaaaaagc tacgagttc gatcccgaa aagtaccga gacgcggtt gtcatgccc tggtaccgc aaccaagat caaccccag tatttctac gtcgcggaa atttgctcc aacttaaac ccggcctcg gactttcca ggctccgac tacgccact ttcgaggag tactacttg aggaaatac agcatacag atacagaat aaaagccaa cacttgctg gacgttgac catacgtcg gccaggttg aatttccta accccgagg tatgtcaac aggaaggga gtggcttta ccgaccagt agcgaggcc acgaaacga gcaaaacgc gagaaactg gaacagaag caaattctc gtccccgaa ttgtgcgcc atccacccg ttttctgcc tctttgtgg cgaaaagcc gtatgttta ccctgcatc ttgtacagg atcaacgca ctgctattg gctgatcaa atcaggcga acagttgcg ttggagctg aatttgggc aagatcgag ctggattca gagttcaaa tggccaccg ttaaacttc ggatggtcc ttggccgac gtcttgaag aagtccaaa gacgaggag aagaagaaa caggagaaa atcgaacca gtcattgaa gaaatccca tgtactgag atagcaaag atcgaggat tttgaccaa gatgacgac gaggaagaa atgattgag attgggact tggtcgaat gacatggct caattaaac agcgaccaa gagtttccc gttgtgagg tacgcctca ccgaccagt tggatggac ctccaaaac acttacgac gattcgtcc ttttcggac tcggactac tccggcgat gagtccgag tccgaatgg ggcggactg cgtatcgaa ttcacgggt gataacgta gccgaagcc gtcgatgac gagaacaag aaagacgac gatttcgaa ctcgtcgac tactccaat gtatggaaa gtcgaagac gagagcgaa atcacgcaa acactgcgg aagcagttc catgacgcg tgtgcccgt aacaaagat cacattctc tcttcaggc attctagtt tcaaagtcg gaacagttc cagaaatgt tccgactgt gacaacacc acgacgaaa gattcgcaa gttgcgaat agttacgac tttgacttc ggtaaatta tttatcgag ctcgatcaa cacaaggcg ttacaaatc gacttggcg ccgcaagat gccaggaat gagtacgac atatccgaa accatgact tttaagttc gacgaacaa ccgaatttg gtcgaacat cccggacca agtccgaac gcgttacaa atcgacttg gcgccgcaa gatgccagg aatgagtac gacatatcc gaaaccatg acttttaag ttcgacgaa caaccgaat ttggtcgaa catcccgga ccaagtccg aacgtacta ctgcaagca ctgacgatg tcgaatgcc aacgatggg attaactta gagcgactg gaaacgatc ggtgactcg ttcctcaaa tacgcaatc acgaactac ttgtactcg aagtacgag aatgtgcac gagggcaaa ttgagtcac cttaggtcg aagcaagtg agtaacctt aatttgtac cgactgggg cggcgaaaa ggcttgggg gaatacatg atcgcgact aagttcgac cctcatgac aattggctg ccgccgtgc ttctacgtc ccgaaggag ctcgaagag gcgttgatt gatgcccag ttccctgcc aactgctgg acggtggcg gacatggcc gctacgaga gacatgact ttggatgat atatgttcg atggttcgc caaagaggc gaaagtctt tcgcttagt aacataatc ccgtacaat ttggtgacg cagcacagt atccccgat aagagcatc gccgattgt gtcgaggct ctaatcggg gcgtatctg atcgaatgt gggcctcgc ggagctttg ctttttatg gcgtggtta ggaatacga gttttgcca cagttggaa gacggcact tatggcgag attgaatta ccgaagtcg cccctgtcg aatcacttg acatatccg cgcgaggag ttggacatg cttttggac ggctatgac cagtttgaa cgacacata gggtataag ttccgcgac cggtcgtac ctactccaa gctttaacc catgcttcg ttctcacca aatactctg actgactgt tatcaaaga cttgagttt ctcggtgat gccgttctt gactactta atcacccgg catttgtac gaagacact aggatgcat tcaccggga gctttgact gatttaagg tccgcctta gtaaacaat acgatattc gcgtcgttg gcagttcgc aacggcttc caccgctac ttccgcaac ctatcgcca agcctgaac gaagtggtc gaaaagttc gtccgactg caagaagac agcggccac accctcgtc gacgaactg tacctagtt gtggagacc gaagaggtc gaagacgtc gaagtcccg aaagccttg ggcgacgtg ttcgagtcc gtcgccggc gcgatattc ctcgactcg ggaatgtcg ctggacgcc gtttggaag gtttactac aacatgatg aagagcgag atcgagcag tttagcaac aaagtcccc aagtcgccg atccgcgaa ctgctcgag ctcgaaccg gagacggcg aagttcgga aaaccggag aaactggct gacggacga cgcgtccgc gtcacggtg gaagtcttc gggaagggg gtgttcaaa ggcatcggg aggaactac cgaatcgcc aaatgcaca gctgcgaaa tgtgcgctc aaaaatttg aaaaaacga ggcttgatc aaaaaaatt gacgaaagt taa
- >TC001750 RA
tgttgtttg aatctaaac tggtatttt tgtgataat ttgtatatt ccaagtgag aaaagatgt atcccgttg gtggtgaga cgtctctaa gttagttca atcataatc agcaataat agcaacgaa aaatgctta gataagtcc tccgttgtg tacagcacc cctacatgg cttgttatt taaacgaaa atgtttaca cgcacacgt ttaccccac gagagtatc aagtggagc tgctggaca gcgctaaga agcgtaata caatcgtat gttcgagtg cgagttcgg cgaaagcgt ttataacaa taaaactgc tgcaagagt tctcccaca agatgcggg tgccccacg gaaagcagg ctttattcg tgctggacg gcccgaacg tcccgataa tgacgtcgc acgtgaagc tgctgaccg atctgaccg ttacgagca tagataaag aggaaaacc ccccgtcgt tgaaggcaa gcaatgtga ttgtgacca cagccgaag tctgtgtgc ttctatgta agaagaact tcgttcatt tggacagtt acgctctaa tcgtgatag actgcttat acggggggc aacagtcgt tagttaggg aaattatgg cccggtatc aggcgattc aagcgccca gacctcgaa ttttgggcc tgactgccg gtcttttgg gctccgaga tgcaacccg atcgtctag aagccgaat tacaacgct tagaaaaat tacttagtt cctcggtag acacttcta gtgaaattc tcacgctga tacgcttat cttgtcggc cgcgcgaac gcatagtcg agtgcttta aacccattc caagccctt tgcaggaca aaatcaagg ccactataa cctcctgtc aggatttcc tcaaggacc accgctacg atccgagcg aaatctacg acgatgact tgctcgagg aattcaagc aagtcccag accccaagg aacagcctt tgagcttct tcgacgact tcctcgaaa ttctggacg atttggggc cctggtcgg ccgacagag ccgcctatg gcatgctta tcaagatcg agaagctca aagtcaaag ttccttacg aaagacatt atttattgt tatgcgttg cttcatcag tattagttt cgataaggg ctctctgcg aactcgaat tccaagact acacagata aggaaaagg tgtttcgtt tctcaacgc cgaaagtac tcagatttc tccaagttc taaagcagt ttaaaccga ccggtgata agccggaaa cttgcgaca aactaccag acctcaaag accccaaga agggaaaag ggaaaaact acaaagggc cccgaaggc cgtacatct cgcgcgcgc aaagcgacg agatgctct gcgcactcg ttttcgtca aaaatcgct acaaggcgg aggccctct tcgccctac tgtgtgtga tgtcaaaga gcgacgagg agtattggt gggtgtcgg tttcgttct ccgtgaata agatcgcag atccggtgc gcgagcctc gggaggcgg aatccgagc ataaaaggc aggaggaag ttttgcgga agtacagaa gtcacgaat gcaatataa tgatagcaa cgtcggcgt tagaacaag gctgtgatc ttcctaaat gtaatttag tgatacgtt tcgatttac ctcagtcgt tccatagct acattcata gtaaagcgc gagcgcgcg caaacgaag ctcattttc ttttgctag cgaacgaga acgaagtta gcgattttg ttgagaatt tagccgagt acaacgaag ttgagaata cgctattga agaggtgtt acagtttgg aacctgata agaatgagg agctggttg ctgatgctt cgtctctgc aatgtagac cgtatcagc cgtcggccg aaccggggg ctaactcag ttagtctct cgaatgcta tagctttag tcaaccggt attgtgcaa aattgccca gcgatacgt tcacccgct tgacgccaa tttggcatg aggagaagg tcgagaatg gttatattt gttccattc ggttaccta tcaattctc cggtgaaga aaacggtga caagccctc caatgatta atactttac tggcacgca gagctgcag cttttatga tttgccaac tcctacaca aagcggggg aattagacg ataatctgc aaccaatag gcaaagaga atttcaaag tgaacgaag aagactgga atagctcgg cgcttgaag aatcagatg aagaaaatc tagacccga gaccgggaa ctacgaaac ggcggcagt attactata aaaaagttg ccgatgctc tacttgact gtcacccaa ttattggcc aaccgactt acttttaca aaatcgtaa tgaaactga cctgccctt tgcccgaag aacaaaaca ccagggggc ggaaaatat accctccgg aggactctc ctcaagggt ttggaatac tgacctcca aagaaatac caaagataa gtgcctttc caattttca caagatcgg gtgaagtaa gtgttgatt tacaattgt gttcacagt taattgtaa cggagaatc aaatttgca aaattaggg aatttttaa attacacgt ttactagtg ttttacgtt tacaaaagt acctaaccc ttttcaatc ctgacgctt cggccaatt cgtacttaa tcgtgccaa cgatagatg gtgccacca ccaccgtcg actgggact tcatcgact taatctacg ccaacttga cggtactcc ccgagataa ttccggaag aagtccgaa aaagctacg agttcgatc ccgaaaagt accgagacg cggttgtca tgccctggt accgcaacc aagatcaac cccagtatt tctacgtcg cggaaattt gctccaact taaacccgg cctcggact ttccaggct ccgactacg ccactttcg aggagtact acttgagga aatacagca tacagatac agaataaaa gccaacact tgctggacg ttgaccata cgtcggcca ggttgaatt tcctaaccc cgaggtatg tcaacagga agggagtgg ctttaccga ccagtagcg aggccacga aacgagcaa aacgcgaga aactggaac agaagcaaa ttctcgtcc ccgaattgt gcgccatcc acccgtttt ctgcctctt tgtggcgaa aagccgtat gtttaccct gcatcttgt acaggatca acgcactgc tattggctg atcaaatca ggcgaacag ttgcgttgg agctgaatt tgggcaaga tcgagctgg attcagagt tcaaatggc caccgttaa acttcggat ggtccttgg ccgacgtct tgaagaagt ccaaagacg aggagaaga agaaacagg agaaaatcg aaccagtca ttgaagaaa tcccatgta ctgagatag caaagatcg aggattttg accaagatg acgacgagg aagaaatga ttgagattg ggacttggt cgaatgaca tggctcaat taaacagcg accaagagt ttcccgttg tgaggtacg cctcaccga ccagttgga tggacctcc aaaacactt acgacgatt cgtcctttt cggactcgg actactccg gcgatgagt ccgagtccg aatggggcg gactgcgta tcgaattca cgggtgata acgtagccg aagccgtcg atgacgaga acaagaaag acgacgatt tcgaactcg tcgactact ccaatgtat ggaaagtcg aagacgaga gcgaaatca cgcaaacac tgcggaagc agttccatg acgcgtgtg cccgtaaca aagatcaca ttctctctt caggcattc tagtttcaa agtcggaac agttccaga aatgttccg actgtgaca acaccacga cgaaagatt cgcaagttg cgaatagtt acgactttg acttcggta aattattta tcgagctcg atcaacaca aggcgttac aaatcgact tggcgccgc aagatgcca ggaatgagt acgacatat ccgaaacca tgactttta agttcgacg aacaaccga atttggtcg aacatcccg gaccaagtc cgaacgcgt tacaaatcg acttggcgc cgcaagatg ccaggaatg agtacgaca tatccgaaa ccatgactt ttaagttcg acgaacaac cgaatttgg tcgaacatc ccggaccaa gtccgaacg tactactgc aagcactga cgatgtcga atgccaacg atgggatta acttagagc gactggaaa cgatcggtg actcgttcc tcaaatacg caatcacga actacttgt actcgaagt acgagaatg tgcacgagg gcaaattga gtcacctta ggtcgaagc aagtgagta accttaatt tgtaccgac tggggcggc gaaaaggct tgggggaat acatgatcg cgactaagt tcgaccctc atgacaatt ggctgccgc cgtgcttct acgtcccga aggagctcg aagaggcgt tgattgatg cccagttcc ctgccaact gctggacgg tggcggaca tggccgcta cgagagaca tgactttgg atgatatat gttcgatgg ttcgccaaa gaggcgaaa gtctttcgc ttagtaaca taatcccgt acaatttgg tgacgcagc acagtatcc ccgataaga gcatcgccg attgtgtcg aggctctaa tcggggcgt atctgatcg aatgtgggc ctcgcggag ctttgcttt ttatggcgt ggttaggaa tacgagttt tgccacagt tggaagacg gcacttatg gcgagattg aattaccga agtcgcccc tgtcgaatc acttgacat atccgcgcg aggagttgg acatgcttt tggacggct atgaccagt ttgaacgac acatagggt ataagttcc gcgaccggt cgtacctac tccaagctt taacccatg cttcgttct caccaaata ctctgactg actgttatc aaagacttg agtttctcg gtgatgccg ttcttgact acttaatca cccggcatt tgtacgaag acactagga tgcattcac cgggagctt tgactgatt taaggtccg ccttagtaa acaatacga tattcgcgt cgttggcag ttcgcaacg gcttccacc gctacttcc gcaacctat cgccaagcc tgaacgaag tggtcgaaa agttcgtcc gactgcaag aagacagcg gccacaccc tcgtcgacg aactgtacc tagttgtgg agaccgaag aggtcgaag acgtcgaag tcccgaaag ccttgggcg acgtgttcg agtccgtcg ccggcgcga tattcctcg actcgggaa tgtcgctgg acgccgttt ggaaggttt actacaaca tgatgaaga gcgagatcg agcagttta gcaacaaag tccccaagt cgccgatcc gcgaactgc tcgagctcg aaccggaga cggcgaagt tcggaaaac cggagaaac tggctgacg gacgacgcg tccgcgtca cggtggaag tcttcggga agggggtgt tcaaaggca tcgggagga actaccgaa tcgccaaat gcacagctg cgaaatgtg cgctcaaaa atttgaaaa aacgaggct tgatcaaaa aaattgacg aaagttaag tgccatcgg caaatacaa caaacaatt ctagtcatt atttcgcgg tgaatttta gtgtgcaaa ataggcctt aaaaatttc cagtaatct gtgatgtag tcgcacaaa atcgcaatg gcagctgag ttttatcgt tttaattgg ttcgactcg cagatttat ctttacgat gtgcatata aagatcgtt tatcactta accaagaaa tgtttgttc aaaaatgtg atataactg ctcgttgtg atagaaacg aagataaat aggttatcc actatagtg tagagtttc tattatttt ttctccgtt tgtaaatat tgcgttttg gatgtaata cagactctt cctaatg
