Status
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Gene information
General information
TC001750 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7986862..7994207
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001750
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09437

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    tropharium structure irregular - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    egg chamber stage sequence irregular - Number of animals showing the phenotype: 4

    egg chamber potentially number decreased - Number of animals showing the phenotype: 4
    vitellarium potentially size decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001750 RA
      MACYLNENV YTHTFTPRE YQVELLDSA KKRNTIVCS 
      SASSAKAFI TIKLLQEFS HKMRVPHGK QALFVLDGP 
      NVPIMTSHV KLLTDLTVT SIDKEENPP SLKASNVIV 
      TTAEVCVLL CKKNFVHLD SYALIVIDC LYGGQQSLV 
      REIMARYQA IQAPRPRIL GLTAGLLGS EMQPDRLEA 
      ELQRLEKLL SSSVDTSSE ILTLIRLSC RPRERIVEC 
      FKPIPSPLQ DKIKATITS CQDFLKDHR YDPSEIYDD 
      DLLEEFKQV PDPKEQPLS FFDDFLEIL DDLGPWSAD 
      RAAYGMLIK IEKLKVKVP YERHYLLLC VASSVLVSI 
      RALCELEFQ DYTDKEKVF RFSTPKVLR FLQVLKQFK 
      PTGDKPETC DKLPDLKDP KKGKGKNYK GPRRPYISR 
      AQSDEMLCA LVFVKNRYK AEALFALLC VMSKSDEEY 
      WWVSVSFSV NKIADPVRE PREAESEHK RQEEVLRKY 
      RSHECNIMI ATSALEQGC DLPKCNLVI RFDLPQSFH 
      SYIHSKARA RANEAHFLL LANENEVSD FVENLAEYN 
      EVENTLLKR CYSLEPDKN EELVADASS LQCRPYQPS 
      AEPGANSVS LSNAIALVN RYCAKLPSD TFTRLTPIW 
      HEEKVENGY ICSIRLPIN SPVKKTVTS PPMINTLLA 
      RRAAAFMIC QLLHKAGEL DDNLQPIGK ENFKVNEED 
      WNSSALEES DEENLDPRP GTTKRRQYY YKKVADALL 
      DCHPIIGQP TYFYKIVMK LTCPLPEEQ NTRGRKIYP 
      PEDSPQGFG ILTSKEIPK ISAFPIFTR SGEVSVDLQ 
      LCSQLIVTE NQICKIREF LNYTFTSVL RLQKYLTLF 
      NPDASANSY LIVPTIDGA TTTVDWDFI DLIYANLTV 
      LPEIIPEEV RKSYEFDPE KYRDAVVMP WYRNQDQPQ 
      YFYVAEICS NLNPASDFP GSDYATFEE YYLRKYSIQ 
      IQNKSQHLL DVDHTSARL NFLTPRYVN RKGVALPTS 
      SEATKRAKR EKLEQKQIL VPELCAIHP FSASLWRKA 
      VCLPCILYR INALLLADQ IRRTVALEL NLGKIELDS 
      EFKWPPLNF GWSLADVLK KSKDEEKKK QEKIEPVIE 
      EIPCTEIAK IEDFDQDDD EEEMIEIGT WSNDMAQLN 
      SDQEFPVVR YASPTSWMD LQNTYDDSS FSDSDYSGD 
      ESESEWGGL RIEFTGDNV AEAVDDENK KDDDFELVD 
      YSNVWKVED ESEITQTLR KQFHDACAR NKDHILSSG 
      ILVSKSEQF QKCSDCDNT TTKDSQVAN SYDFDFGKL 
      FIELDQHKA LQIDLAPQD ARNEYDISE TMTFKFDEQ 
      PNLVEHPGP SPNALQIDL APQDARNEY DISETMTFK 
      FDEQPNLVE HPGPSPNVL LQALTMSNA NDGINLERL 
      ETIGDSFLK YAITNYLYS KYENVHEGK LSHLRSKQV 
      SNLNLYRLG RRKGLGEYM IATKFDPHD NWLPPCFYV 
      PKELEEALI DAQFPANCW TVADMAATR DMTLDDICS 
      MVRQRGESL SLSNIIPYN LVTQHSIPD KSIADCVEA 
      LIGAYLIEC GPRGALLFM AWLGIRVLP QLEDGTYGE 
      IELPKSPLS NHLTYPREE LDMLLDGYD QFERHIGYK 
      FRDRSYLLQ ALTHASFSP NTLTDCYQR LEFLGDAVL 
      DYLITRHLY EDTRMHSPG ALTDLRSAL VNNTIFASL 
      AVRNGFHRY FRNLSPSLN EVVEKFVRL QEDSGHTLV 
      DELYLVVET EEVEDVEVP KALGDVFES VAGAIFLDS 
      GMSLDAVWK VYYNMMKSE IEQFSNKVP KSPIRELLE 
      LEPETAKFG KPEKLADGR RVRVTVEVF GKGVFKGIG 
      RNYRIAKCT AAKCALKNL KKRGLIKKI DES
    CDS sequence
    • >TC001750 RA
      atggcttgt tatttaaac gaaaatgtt tacacgcac 
      acgtttacc ccacgagag tatcaagtg gagctgctg 
      gacagcgct aagaagcgt aatacaatc gtatgttcg 
      agtgcgagt tcggcgaaa gcgtttata acaataaaa 
      ctgctgcaa gagttctcc cacaagatg cgggtgccc 
      cacggaaag caggcttta ttcgtgctg gacggcccg 
      aacgtcccg ataatgacg tcgcacgtg aagctgctg 
      accgatctg accgttacg agcatagat aaagaggaa 
      aaccccccg tcgttgaag gcaagcaat gtgattgtg 
      accacagcc gaagtctgt gtgcttcta tgtaagaag 
      aacttcgtt catttggac agttacgct ctaatcgtg 
      atagactgc ttatacggg gggcaacag tcgttagtt 
      agggaaatt atggcccgg tatcaggcg attcaagcg 
      cccagacct cgaattttg ggcctgact gccggtctt 
      ttgggctcc gagatgcaa cccgatcgt ctagaagcc 
      gaattacaa cgcttagaa aaattactt agttcctcg 
      gtagacact tctagtgaa attctcacg ctgatacgc 
      ttatcttgt cggccgcgc gaacgcata gtcgagtgc 
      tttaaaccc attccaagc cctttgcag gacaaaatc 
      aaggccact ataacctcc tgtcaggat ttcctcaag 
      gaccaccgc tacgatccg agcgaaatc tacgacgat 
      gacttgctc gaggaattc aagcaagtc ccagacccc 
      aaggaacag cctttgagc ttcttcgac gacttcctc 
      gaaattctg gacgatttg gggccctgg tcggccgac 
      agagccgcc tatggcatg cttatcaag atcgagaag 
      ctcaaagtc aaagttcct tacgaaaga cattattta 
      ttgttatgc gttgcttca tcagtatta gtttcgata 
      agggctctc tgcgaactc gaattccaa gactacaca 
      gataaggaa aaggtgttt cgtttctca acgccgaaa 
      gtactcaga tttctccaa gttctaaag cagtttaaa 
      ccgaccggt gataagccg gaaacttgc gacaaacta 
      ccagacctc aaagacccc aagaaggga aaagggaaa 
      aactacaaa gggccccga aggccgtac atctcgcgc 
      gcgcaaagc gacgagatg ctctgcgca ctcgttttc 
      gtcaaaaat cgctacaag gcggaggcc ctcttcgcc 
      ctactgtgt gtgatgtca aagagcgac gaggagtat 
      tggtgggtg tcggtttcg ttctccgtg aataagatc 
      gcagatccg gtgcgcgag cctcgggag gcggaatcc 
      gagcataaa aggcaggag gaagttttg cggaagtac 
      agaagtcac gaatgcaat ataatgata gcaacgtcg 
      gcgttagaa caaggctgt gatcttcct aaatgtaat 
      ttagtgata cgtttcgat ttacctcag tcgttccat 
      agctacatt catagtaaa gcgcgagcg cgcgcaaac 
      gaagctcat tttcttttg ctagcgaac gagaacgaa 
      gttagcgat tttgttgag aatttagcc gagtacaac 
      gaagttgag aatacgcta ttgaagagg tgttacagt 
      ttggaacct gataagaat gaggagctg gttgctgat 
      gcttcgtct ctgcaatgt agaccgtat cagccgtcg 
      gccgaaccg ggggctaac tcagttagt ctctcgaat 
      gctatagct ttagtcaac cggtattgt gcaaaattg 
      cccagcgat acgttcacc cgcttgacg ccaatttgg 
      catgaggag aaggtcgag aatggttat atttgttcc 
      attcggtta cctatcaat tctccggtg aagaaaacg 
      gtgacaagc cctccaatg attaatact ttactggca 
      cgcagagct gcagctttt atgatttgc caactccta 
      cacaaagcg ggggaatta gacgataat ctgcaacca 
      ataggcaaa gagaatttc aaagtgaac gaagaagac 
      tggaatagc tcggcgctt gaagaatca gatgaagaa 
      aatctagac ccgagaccg ggaactacg aaacggcgg 
      cagtattac tataaaaaa gttgccgat gctctactt 
      gactgtcac ccaattatt ggccaaccg acttacttt 
      tacaaaatc gtaatgaaa ctgacctgc cctttgccc 
      gaagaacaa aacaccagg gggcggaaa atataccct 
      ccggaggac tctcctcaa gggtttgga atactgacc 
      tccaaagaa ataccaaag ataagtgcc tttccaatt 
      ttcacaaga tcgggtgaa gtaagtgtt gatttacaa 
      ttgtgttca cagttaatt gtaacggag aatcaaatt 
      tgcaaaatt agggaattt ttaaattac acgtttact 
      agtgtttta cgtttacaa aagtaccta acccttttc 
      aatcctgac gcttcggcc aattcgtac ttaatcgtg 
      ccaacgata gatggtgcc accaccacc gtcgactgg 
      gacttcatc gacttaatc tacgccaac ttgacggta 
      ctccccgag ataattccg gaagaagtc cgaaaaagc 
      tacgagttc gatcccgaa aagtaccga gacgcggtt 
      gtcatgccc tggtaccgc aaccaagat caaccccag 
      tatttctac gtcgcggaa atttgctcc aacttaaac 
      ccggcctcg gactttcca ggctccgac tacgccact 
      ttcgaggag tactacttg aggaaatac agcatacag 
      atacagaat aaaagccaa cacttgctg gacgttgac 
      catacgtcg gccaggttg aatttccta accccgagg 
      tatgtcaac aggaaggga gtggcttta ccgaccagt 
      agcgaggcc acgaaacga gcaaaacgc gagaaactg 
      gaacagaag caaattctc gtccccgaa ttgtgcgcc 
      atccacccg ttttctgcc tctttgtgg cgaaaagcc 
      gtatgttta ccctgcatc ttgtacagg atcaacgca 
      ctgctattg gctgatcaa atcaggcga acagttgcg 
      ttggagctg aatttgggc aagatcgag ctggattca 
      gagttcaaa tggccaccg ttaaacttc ggatggtcc 
      ttggccgac gtcttgaag aagtccaaa gacgaggag 
      aagaagaaa caggagaaa atcgaacca gtcattgaa 
      gaaatccca tgtactgag atagcaaag atcgaggat 
      tttgaccaa gatgacgac gaggaagaa atgattgag 
      attgggact tggtcgaat gacatggct caattaaac 
      agcgaccaa gagtttccc gttgtgagg tacgcctca 
      ccgaccagt tggatggac ctccaaaac acttacgac 
      gattcgtcc ttttcggac tcggactac tccggcgat 
      gagtccgag tccgaatgg ggcggactg cgtatcgaa 
      ttcacgggt gataacgta gccgaagcc gtcgatgac 
      gagaacaag aaagacgac gatttcgaa ctcgtcgac 
      tactccaat gtatggaaa gtcgaagac gagagcgaa 
      atcacgcaa acactgcgg aagcagttc catgacgcg 
      tgtgcccgt aacaaagat cacattctc tcttcaggc 
      attctagtt tcaaagtcg gaacagttc cagaaatgt 
      tccgactgt gacaacacc acgacgaaa gattcgcaa 
      gttgcgaat agttacgac tttgacttc ggtaaatta 
      tttatcgag ctcgatcaa cacaaggcg ttacaaatc 
      gacttggcg ccgcaagat gccaggaat gagtacgac 
      atatccgaa accatgact tttaagttc gacgaacaa 
      ccgaatttg gtcgaacat cccggacca agtccgaac 
      gcgttacaa atcgacttg gcgccgcaa gatgccagg 
      aatgagtac gacatatcc gaaaccatg acttttaag 
      ttcgacgaa caaccgaat ttggtcgaa catcccgga 
      ccaagtccg aacgtacta ctgcaagca ctgacgatg 
      tcgaatgcc aacgatggg attaactta gagcgactg 
      gaaacgatc ggtgactcg ttcctcaaa tacgcaatc 
      acgaactac ttgtactcg aagtacgag aatgtgcac 
      gagggcaaa ttgagtcac cttaggtcg aagcaagtg 
      agtaacctt aatttgtac cgactgggg cggcgaaaa 
      ggcttgggg gaatacatg atcgcgact aagttcgac 
      cctcatgac aattggctg ccgccgtgc ttctacgtc 
      ccgaaggag ctcgaagag gcgttgatt gatgcccag 
      ttccctgcc aactgctgg acggtggcg gacatggcc 
      gctacgaga gacatgact ttggatgat atatgttcg 
      atggttcgc caaagaggc gaaagtctt tcgcttagt 
      aacataatc ccgtacaat ttggtgacg cagcacagt 
      atccccgat aagagcatc gccgattgt gtcgaggct 
      ctaatcggg gcgtatctg atcgaatgt gggcctcgc 
      ggagctttg ctttttatg gcgtggtta ggaatacga 
      gttttgcca cagttggaa gacggcact tatggcgag 
      attgaatta ccgaagtcg cccctgtcg aatcacttg 
      acatatccg cgcgaggag ttggacatg cttttggac 
      ggctatgac cagtttgaa cgacacata gggtataag 
      ttccgcgac cggtcgtac ctactccaa gctttaacc 
      catgcttcg ttctcacca aatactctg actgactgt 
      tatcaaaga cttgagttt ctcggtgat gccgttctt 
      gactactta atcacccgg catttgtac gaagacact 
      aggatgcat tcaccggga gctttgact gatttaagg 
      tccgcctta gtaaacaat acgatattc gcgtcgttg 
      gcagttcgc aacggcttc caccgctac ttccgcaac 
      ctatcgcca agcctgaac gaagtggtc gaaaagttc 
      gtccgactg caagaagac agcggccac accctcgtc 
      gacgaactg tacctagtt gtggagacc gaagaggtc 
      gaagacgtc gaagtcccg aaagccttg ggcgacgtg 
      ttcgagtcc gtcgccggc gcgatattc ctcgactcg 
      ggaatgtcg ctggacgcc gtttggaag gtttactac 
      aacatgatg aagagcgag atcgagcag tttagcaac 
      aaagtcccc aagtcgccg atccgcgaa ctgctcgag 
      ctcgaaccg gagacggcg aagttcgga aaaccggag 
      aaactggct gacggacga cgcgtccgc gtcacggtg 
      gaagtcttc gggaagggg gtgttcaaa ggcatcggg 
      aggaactac cgaatcgcc aaatgcaca gctgcgaaa 
      tgtgcgctc aaaaatttg aaaaaacga ggcttgatc 
      aaaaaaatt gacgaaagt taa
    mRNA sequence
    • >TC001750 RA
      tgttgtttg aatctaaac tggtatttt tgtgataat 
      ttgtatatt ccaagtgag aaaagatgt atcccgttg 
      gtggtgaga cgtctctaa gttagttca atcataatc 
      agcaataat agcaacgaa aaatgctta gataagtcc 
      tccgttgtg tacagcacc cctacatgg cttgttatt 
      taaacgaaa atgtttaca cgcacacgt ttaccccac 
      gagagtatc aagtggagc tgctggaca gcgctaaga 
      agcgtaata caatcgtat gttcgagtg cgagttcgg 
      cgaaagcgt ttataacaa taaaactgc tgcaagagt 
      tctcccaca agatgcggg tgccccacg gaaagcagg 
      ctttattcg tgctggacg gcccgaacg tcccgataa 
      tgacgtcgc acgtgaagc tgctgaccg atctgaccg 
      ttacgagca tagataaag aggaaaacc ccccgtcgt 
      tgaaggcaa gcaatgtga ttgtgacca cagccgaag 
      tctgtgtgc ttctatgta agaagaact tcgttcatt 
      tggacagtt acgctctaa tcgtgatag actgcttat 
      acggggggc aacagtcgt tagttaggg aaattatgg 
      cccggtatc aggcgattc aagcgccca gacctcgaa 
      ttttgggcc tgactgccg gtcttttgg gctccgaga 
      tgcaacccg atcgtctag aagccgaat tacaacgct 
      tagaaaaat tacttagtt cctcggtag acacttcta 
      gtgaaattc tcacgctga tacgcttat cttgtcggc 
      cgcgcgaac gcatagtcg agtgcttta aacccattc 
      caagccctt tgcaggaca aaatcaagg ccactataa 
      cctcctgtc aggatttcc tcaaggacc accgctacg 
      atccgagcg aaatctacg acgatgact tgctcgagg 
      aattcaagc aagtcccag accccaagg aacagcctt 
      tgagcttct tcgacgact tcctcgaaa ttctggacg 
      atttggggc cctggtcgg ccgacagag ccgcctatg 
      gcatgctta tcaagatcg agaagctca aagtcaaag 
      ttccttacg aaagacatt atttattgt tatgcgttg 
      cttcatcag tattagttt cgataaggg ctctctgcg 
      aactcgaat tccaagact acacagata aggaaaagg 
      tgtttcgtt tctcaacgc cgaaagtac tcagatttc 
      tccaagttc taaagcagt ttaaaccga ccggtgata 
      agccggaaa cttgcgaca aactaccag acctcaaag 
      accccaaga agggaaaag ggaaaaact acaaagggc 
      cccgaaggc cgtacatct cgcgcgcgc aaagcgacg 
      agatgctct gcgcactcg ttttcgtca aaaatcgct 
      acaaggcgg aggccctct tcgccctac tgtgtgtga 
      tgtcaaaga gcgacgagg agtattggt gggtgtcgg 
      tttcgttct ccgtgaata agatcgcag atccggtgc 
      gcgagcctc gggaggcgg aatccgagc ataaaaggc 
      aggaggaag ttttgcgga agtacagaa gtcacgaat 
      gcaatataa tgatagcaa cgtcggcgt tagaacaag 
      gctgtgatc ttcctaaat gtaatttag tgatacgtt 
      tcgatttac ctcagtcgt tccatagct acattcata 
      gtaaagcgc gagcgcgcg caaacgaag ctcattttc 
      ttttgctag cgaacgaga acgaagtta gcgattttg 
      ttgagaatt tagccgagt acaacgaag ttgagaata 
      cgctattga agaggtgtt acagtttgg aacctgata 
      agaatgagg agctggttg ctgatgctt cgtctctgc 
      aatgtagac cgtatcagc cgtcggccg aaccggggg 
      ctaactcag ttagtctct cgaatgcta tagctttag 
      tcaaccggt attgtgcaa aattgccca gcgatacgt 
      tcacccgct tgacgccaa tttggcatg aggagaagg 
      tcgagaatg gttatattt gttccattc ggttaccta 
      tcaattctc cggtgaaga aaacggtga caagccctc 
      caatgatta atactttac tggcacgca gagctgcag 
      cttttatga tttgccaac tcctacaca aagcggggg 
      aattagacg ataatctgc aaccaatag gcaaagaga 
      atttcaaag tgaacgaag aagactgga atagctcgg 
      cgcttgaag aatcagatg aagaaaatc tagacccga 
      gaccgggaa ctacgaaac ggcggcagt attactata 
      aaaaagttg ccgatgctc tacttgact gtcacccaa 
      ttattggcc aaccgactt acttttaca aaatcgtaa 
      tgaaactga cctgccctt tgcccgaag aacaaaaca 
      ccagggggc ggaaaatat accctccgg aggactctc 
      ctcaagggt ttggaatac tgacctcca aagaaatac 
      caaagataa gtgcctttc caattttca caagatcgg 
      gtgaagtaa gtgttgatt tacaattgt gttcacagt 
      taattgtaa cggagaatc aaatttgca aaattaggg 
      aatttttaa attacacgt ttactagtg ttttacgtt 
      tacaaaagt acctaaccc ttttcaatc ctgacgctt 
      cggccaatt cgtacttaa tcgtgccaa cgatagatg 
      gtgccacca ccaccgtcg actgggact tcatcgact 
      taatctacg ccaacttga cggtactcc ccgagataa 
      ttccggaag aagtccgaa aaagctacg agttcgatc 
      ccgaaaagt accgagacg cggttgtca tgccctggt 
      accgcaacc aagatcaac cccagtatt tctacgtcg 
      cggaaattt gctccaact taaacccgg cctcggact 
      ttccaggct ccgactacg ccactttcg aggagtact 
      acttgagga aatacagca tacagatac agaataaaa 
      gccaacact tgctggacg ttgaccata cgtcggcca 
      ggttgaatt tcctaaccc cgaggtatg tcaacagga 
      agggagtgg ctttaccga ccagtagcg aggccacga 
      aacgagcaa aacgcgaga aactggaac agaagcaaa 
      ttctcgtcc ccgaattgt gcgccatcc acccgtttt 
      ctgcctctt tgtggcgaa aagccgtat gtttaccct 
      gcatcttgt acaggatca acgcactgc tattggctg 
      atcaaatca ggcgaacag ttgcgttgg agctgaatt 
      tgggcaaga tcgagctgg attcagagt tcaaatggc 
      caccgttaa acttcggat ggtccttgg ccgacgtct 
      tgaagaagt ccaaagacg aggagaaga agaaacagg 
      agaaaatcg aaccagtca ttgaagaaa tcccatgta 
      ctgagatag caaagatcg aggattttg accaagatg 
      acgacgagg aagaaatga ttgagattg ggacttggt 
      cgaatgaca tggctcaat taaacagcg accaagagt 
      ttcccgttg tgaggtacg cctcaccga ccagttgga 
      tggacctcc aaaacactt acgacgatt cgtcctttt 
      cggactcgg actactccg gcgatgagt ccgagtccg 
      aatggggcg gactgcgta tcgaattca cgggtgata 
      acgtagccg aagccgtcg atgacgaga acaagaaag 
      acgacgatt tcgaactcg tcgactact ccaatgtat 
      ggaaagtcg aagacgaga gcgaaatca cgcaaacac 
      tgcggaagc agttccatg acgcgtgtg cccgtaaca 
      aagatcaca ttctctctt caggcattc tagtttcaa 
      agtcggaac agttccaga aatgttccg actgtgaca 
      acaccacga cgaaagatt cgcaagttg cgaatagtt 
      acgactttg acttcggta aattattta tcgagctcg 
      atcaacaca aggcgttac aaatcgact tggcgccgc 
      aagatgcca ggaatgagt acgacatat ccgaaacca 
      tgactttta agttcgacg aacaaccga atttggtcg 
      aacatcccg gaccaagtc cgaacgcgt tacaaatcg 
      acttggcgc cgcaagatg ccaggaatg agtacgaca 
      tatccgaaa ccatgactt ttaagttcg acgaacaac 
      cgaatttgg tcgaacatc ccggaccaa gtccgaacg 
      tactactgc aagcactga cgatgtcga atgccaacg 
      atgggatta acttagagc gactggaaa cgatcggtg 
      actcgttcc tcaaatacg caatcacga actacttgt 
      actcgaagt acgagaatg tgcacgagg gcaaattga 
      gtcacctta ggtcgaagc aagtgagta accttaatt 
      tgtaccgac tggggcggc gaaaaggct tgggggaat 
      acatgatcg cgactaagt tcgaccctc atgacaatt 
      ggctgccgc cgtgcttct acgtcccga aggagctcg 
      aagaggcgt tgattgatg cccagttcc ctgccaact 
      gctggacgg tggcggaca tggccgcta cgagagaca 
      tgactttgg atgatatat gttcgatgg ttcgccaaa 
      gaggcgaaa gtctttcgc ttagtaaca taatcccgt 
      acaatttgg tgacgcagc acagtatcc ccgataaga 
      gcatcgccg attgtgtcg aggctctaa tcggggcgt 
      atctgatcg aatgtgggc ctcgcggag ctttgcttt 
      ttatggcgt ggttaggaa tacgagttt tgccacagt 
      tggaagacg gcacttatg gcgagattg aattaccga 
      agtcgcccc tgtcgaatc acttgacat atccgcgcg 
      aggagttgg acatgcttt tggacggct atgaccagt 
      ttgaacgac acatagggt ataagttcc gcgaccggt 
      cgtacctac tccaagctt taacccatg cttcgttct 
      caccaaata ctctgactg actgttatc aaagacttg 
      agtttctcg gtgatgccg ttcttgact acttaatca 
      cccggcatt tgtacgaag acactagga tgcattcac 
      cgggagctt tgactgatt taaggtccg ccttagtaa 
      acaatacga tattcgcgt cgttggcag ttcgcaacg 
      gcttccacc gctacttcc gcaacctat cgccaagcc 
      tgaacgaag tggtcgaaa agttcgtcc gactgcaag 
      aagacagcg gccacaccc tcgtcgacg aactgtacc 
      tagttgtgg agaccgaag aggtcgaag acgtcgaag 
      tcccgaaag ccttgggcg acgtgttcg agtccgtcg 
      ccggcgcga tattcctcg actcgggaa tgtcgctgg 
      acgccgttt ggaaggttt actacaaca tgatgaaga 
      gcgagatcg agcagttta gcaacaaag tccccaagt 
      cgccgatcc gcgaactgc tcgagctcg aaccggaga 
      cggcgaagt tcggaaaac cggagaaac tggctgacg 
      gacgacgcg tccgcgtca cggtggaag tcttcggga 
      agggggtgt tcaaaggca tcgggagga actaccgaa 
      tcgccaaat gcacagctg cgaaatgtg cgctcaaaa 
      atttgaaaa aacgaggct tgatcaaaa aaattgacg 
      aaagttaag tgccatcgg caaatacaa caaacaatt 
      ctagtcatt atttcgcgg tgaatttta gtgtgcaaa 
      ataggcctt aaaaatttc cagtaatct gtgatgtag 
      tcgcacaaa atcgcaatg gcagctgag ttttatcgt 
      tttaattgg ttcgactcg cagatttat ctttacgat 
      gtgcatata aagatcgtt tatcactta accaagaaa 
      tgtttgttc aaaaatgtg atataactg ctcgttgtg 
      atagaaacg aagataaat aggttatcc actatagtg 
      tagagtttc tattatttt ttctccgtt tgtaaatat 
      tgcgttttg gatgtaata cagactctt cctaatg
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