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Gene information
General information
TC012214 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4628709..4632995
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012214
    iBeetle screen iB_09436

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012214 RA
      MKWQSNSWI RCGYILVCL LGTALAELG NPYEILGVH 
      RKASQSEIK KAYRQLAKE WHPDKTKDP EAEEKFVKI 
      KQAYELLSD AERRNKFDN KGITEDDFY NNPEHPPFH 
      TSNPFDDIF TAHGAHFNF QENDITFFH KLSISTRQY 
      DKLIVPKSE RTPHLIFFY TDWCFACLQ TAPYCRKLV 
      DKLEPLGVN FVTVHSGRE PALARRLNI HALPCIVLL 
      LDGNVFVYK ETITSIQRI IEFLKNKLP YKLVPKLKH 
      EDVEGFLNG WEDNRVRGL IFEPRQNIR LRYLITAYH 
      FRHRVAFGF VDSLEIRRK YQVPQDMDT VLLFNENTS 
      SPMASLSMK DIPTSTLHH VVSLNQYLA LPRLSSQEV 
      LEALCPCEW NKPRKRLCV VLVTKASTS HDPHRQSFR 
      IYAQNSPYN VEKVRFAYI YHDKQTEFV NSLIPEGED 
      VEPLLRIVI LWRRDTSHV KYEWVNDKW EWENNLNQT 
      TQKLESTIS RLLRSSEAL TYEALVNDL FDEHAKGIF 
      GRIASKVGT FVESVYDGL SKDQILPAL SIVGTIIFI 
      LAVGYFMAY LVRLEEESI QQQKAKAKS GNNNNDSNS 
      SYQPELRLH ELRAEKYNG LVRLLKPGC RTILLVVDM 
      HSCRQLIPP FHKAVWPYR KNKTLMFSY MYIERGLSW 
      YKELLQLSL PEERELNIN PRNCVGTVL SLNGHRKYF 
      CMFHAKHTE RNARKQRSK MPTKFDEKD CESGTFIGF 
      DSDSSESEE ELLLQENLL DGLPNWLDR LFEGTTQRY 
      HINYWPDFP NK
    CDS sequence
    • >TC012214 RA
      atgaaatgg cagtccaat tcgtggata cgttgtggt 
      tatattttg gtttgttta ctcgggact gcgctcgcg 
      gaattaggt aacccctac gaaattctg ggtgttcac 
      agaaaggcg tcacagtca gagataaag aaggcttac 
      aggcagctg gcgaaggaa tggcacccc gacaaaacc 
      aaagacccg gaggctgag gaaaagttt gtgaaaata 
      aaacaggcg tatgagctg ttgtctgac gcggagcgg 
      cggaacaaa ttcgataat aaggggatc acagaagac 
      gatttttac aacaaccct gaacaccct cccttccac 
      actagcaac ccgtttgac gacattttc accgcccat 
      ggggcgcat ttcaacttt caagagaac gatattacg 
      tttttccac aaattgtcg atatccaca aggcaatat 
      gataagtta attgtccca aagagtgag agaacgccc 
      cacttgata ttcttctac accgactgg tgttttgct 
      tgccttcag actgcacca tactgccga aagttggtg 
      gacaagttg gaaccactg ggtgttaat tttgtcact 
      gttcattct ggcagggaa ccagctctg gccagacgc 
      ctcaacatc cacgcttta ccctgcatt gttctgcta 
      cttgatggc aacgtgttt gtctacaag gaaaccatc 
      acaagcatt cagagaatt attgaattc ctcaaaaat 
      aagttaccc tacaagtta gtcccaaag ttgaaacac 
      gaagatgtg gaggggttt ctcaacggt tgggaggac 
      aacagagtt cgggggcta atttttgaa ccgcggcaa 
      aacatcagg ttgcgatat ttgataact gcctaccat 
      ttcagacac cgagttgct tttggtttt gtagattcg 
      cttgaaatt cggcgcaaa tatcaagtc ccgcaagac 
      atggacaca gttttgtta tttaatgaa aatacatcg 
      tctcctatg gccagtctc tcaatgaaa gatatacca 
      acttccact ttacaccac gttgtctcg ttgaatcag 
      taccttgcc ctgccaagg ctgtcgtct caggaagtc 
      ttggaggct ctgtgcccc tgcgagtgg aacaagccc 
      cggaagcgc ctgtgcgtt gttttggtg acgaaggca 
      tcaaccagt catgacccc cacagacaa tctttccga 
      atttacgca caaaattcg ccctacaat gtggaaaaa 
      gtccgtttt gcgtatatc tatcacgac aaacaaaca 
      gagtttgtt aattcttta atcccagaa ggtgaggac 
      gtcgagccg ttgcttcgt atcgtaatt ttgtggcgt 
      cgagacacg tcgcatgtt aaatacgaa tgggttaat 
      gacaaatgg gaatgggaa aataatctt aatcaaaca 
      acacagaaa cttgaaagt acaataagc cgtctgttg 
      aggtctagc gaagctctg acttatgaa gctctagtt 
      aatgattta ttcgatgag cacgcgaag ggaattttc 
      ggccggatt gcgagcaaa gtgggcact tttgtcgag 
      tcggtttat gatggtttg agcaaggat caaatttta 
      ccagcgctg tcgatagtc gggacgatt atttttatc 
      ctggctgtt ggttatttc atggcgtat ttggtgcgg 
      ttggaagaa gagagcatc cagcagcag aaagccaaa 
      gcaaaatct ggaaacaac aataacgat tctaactcg 
      agctaccaa cccgagctg cgtttgcac gaactgcgc 
      gccgagaaa tacaacgga ctggtaaga cttttaaaa 
      cccggttgc cggactatt ctcctagtg gttgatatg 
      cactcatgc cggcaacta atcccaccc ttccataaa 
      gcggtatgg ccataccgg aaaaacaaa actttgatg 
      ttttcctac atgtacatt gaacggggc cttagctgg 
      tacaaggag ttgctgcaa ttgtccttg ccagaggag 
      cgcgaactg aacataaac ccgcgcaac tgcgttggc 
      acagtcttg tccttgaac ggccacaga aaatacttc 
      tgcatgttt cacgccaaa cacactgaa cgcaatgcg 
      aggaagcag agatccaag atgccgacg aaatttgac 
      gaaaaagac tgcgagagt gggactttc atcggcttc 
      gattccgac tcgtctgaa tccgaagag gagcttctg 
      ctacaggag aatctattg gacggtctg ccgaactgg 
      ctcgatcgg ctatttgag ggaacaaca caacgttat 
      cacatcaac tattggcca gattttcca aataaataa
    mRNA sequence
    • >TC012214 RA
      tcaccagtt tgacaattg cctgacatt tgccagtgt 
      gttttcctt gaaaatcaa tattttcac aaatttcgt 
      gctaatttt cgtcaatgt gttgtaact cggtcacat 
      ctcagcatt atatgagct ttaattgac aatgaaatg 
      gcagtccaa ttcgtggat acgttgtgg ttatatttt 
      ggtttgttt actcgggac tgcgctcgc ggaattagg 
      taaccccta cgaaattct gggtgttca cagaaaggc 
      gtcacagtc agagataaa gaaggctta caggcagct 
      ggcgaagga atggcaccc cgacaaaac caaagaccc 
      ggaggctga ggaaaagtt tgtgaaaat aaaacaggc 
      gtatgagct gttgtctga cgcggagcg gcggaacaa 
      attcgataa taaggggat cacagaaga cgattttta 
      caacaaccc tgaacaccc tcccttcca cactagcaa 
      cccgtttga cgacatttt caccgccca tggggcgca 
      tttcaactt tcaagagaa cgatattac gtttttcca 
      caaattgtc gatatccac aaggcaata tgataagtt 
      aattgtccc aaagagtga gagaacgcc ccacttgat 
      attcttcta caccgactg gtgttttgc ttgccttca 
      gactgcacc atactgccg aaagttggt ggacaagtt 
      ggaaccact gggtgttaa ttttgtcac tgttcattc 
      tggcaggga accagctct ggccagacg cctcaacat 
      ccacgcttt accctgcat tgttctgct acttgatgg 
      caacgtgtt tgtctacaa ggaaaccat cacaagcat 
      tcagagaat tattgaatt cctcaaaaa taagttacc 
      ctacaagtt agtcccaaa gttgaaaca cgaagatgt 
      ggaggggtt tctcaacgg ttgggagga caacagagt 
      tcgggggct aatttttga accgcggca aaacatcag 
      gttgcgata tttgataac tgcctacca tttcagaca 
      ccgagttgc ttttggttt tgtagattc gcttgaaat 
      tcggcgcaa atatcaagt cccgcaaga catggacac 
      agttttgtt atttaatga aaatacatc gtctcctat 
      ggccagtct ctcaatgaa agatatacc aacttccac 
      tttacacca cgttgtctc gttgaatca gtaccttgc 
      cctgccaag gctgtcgtc tcaggaagt cttggaggc 
      tctgtgccc ctgcgagtg gaacaagcc ccggaagcg 
      cctgtgcgt tgttttggt gacgaaggc atcaaccag 
      tcatgaccc ccacagaca atctttccg aatttacgc 
      acaaaattc gccctacaa tgtggaaaa agtccgttt 
      tgcgtatat ctatcacga caaacaaac agagtttgt 
      taattcttt aatcccaga aggtgagga cgtcgagcc 
      gttgcttcg tatcgtaat tttgtggcg tcgagacac 
      gtcgcatgt taaatacga atgggttaa tgacaaatg 
      ggaatggga aaataatct taatcaaac aacacagaa 
      acttgaaag tacaataag ccgtctgtt gaggtctag 
      cgaagctct gacttatga agctctagt taatgattt 
      attcgatga gcacgcgaa gggaatttt cggccggat 
      tgcgagcaa agtgggcac ttttgtcga gtcggttta 
      tgatggttt gagcaagga tcaaatttt accagcgct 
      gtcgatagt cgggacgat tatttttat cctggctgt 
      tggttattt catggcgta tttggtgcg gttggaaga 
      agagagcat ccagcagca gaaagccaa agcaaaatc 
      tggaaacaa caataacga ttctaactc gagctacca 
      acccgagct gcgtttgca cgaactgcg cgccgagaa 
      atacaacgg actggtaag acttttaaa acccggttg 
      ccggactat tctcctagt ggttgatat gcactcatg 
      ccggcaact aatcccacc cttccataa agcggtatg 
      gccataccg gaaaaacaa aactttgat gttttccta 
      catgtacat tgaacgggg ccttagctg gtacaagga 
      gttgctgca attgtcctt gccagagga gcgcgaact 
      gaacataaa cccgcgcaa ctgcgttgg cacagtctt 
      gtccttgaa cggccacag aaaatactt ctgcatgtt 
      tcacgccaa acacactga acgcaatgc gaggaagca 
      gagatccaa gatgccgac gaaatttga cgaaaaaga 
      ctgcgagag tgggacttt catcggctt cgattccga 
      ctcgtctga atccgaaga ggagcttct gctacagga 
      gaatctatt ggacggtct gccgaactg gctcgatcg 
      gctatttga gggaacaac acaacgtta tcacatcaa 
      ctattggcc agattttcc aaataaata aacgttaac 
      acatagcct agcctagaa gttgcatag ctcatgtta 
      tttatctgt ttgttgtat ttatacata tctgtaatg 
      tttgtataa ttccatgca tttcaagtg cttgtttct 
      actgttgtg ttgatgtga agatggatc tgttgtgtt 
      tttcattaa ttctagtca catattagg atagccaac 
      tgctgaggg ttgaatgca tccaatttc atattgaaa 
      tttttcaag tggattgtt agcgtagtt tcgaattta 
      tacttcaaa cttacagtc aaaattacg ggagtgctt 
      cgttttggt tttcaaact ccccttgtt aatactcat 
      agcgcctat taaattaaa aaacattta cactagttg 
      gttagtgga cgctataga ttattttaa agtcgtcta 
      tatacgtat ttatttatt tgtgccata tttattaat 
      attttaaga gtaatagtc caaaagaca gtggtgtat 
      tacctcgtg attcaaatt tacaaataa agttgtttt 
      gattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY