Status
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Gene information
General information
TC012221 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 180343..195405
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012221
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09435

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature dorsal lateralthinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09569

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012221 RA
      MATQEKLLK SLEVLKTLS APNQTFDSS KKKDKIQHC 
      HIVTEAISN STIKIAPSF PTYLSITIE VLLQLCDDQ 
      ESDVRMTAG DCLNKVIRA MNDGNIGKV QIELHKGIK 
      QNGSARSLR AALKRFSQL AHLIRPHKG KPYVVNLFP 
      TLIKVSQRP EEQIHETLA NSLAPIMRV LGSFTSEVE 
      IKDLLKGFL KNISHSSPV IRRSATTCV LNVCLNCRK 
      PSIFMAYVI NYLLDLLIP VSDNQSSFL ILGVLACLK 
      NLLQHLNDN QNGDNASLS VDKLLQIYE LCLHYFSNK 
      DHNVVNAAL ETLNVLLQN CSLELRQIL LSESGVDKS 
      RISLPTSYS MSALRSPSQ LSVATSAAN DDLFLESEL 
      TDTIRSDIE KWIDESKLS VMNVPCAKT NELEKSLSS 
      ESKNSSLDV SLAQLDSFD YKISEYVGK SHSVGDNLE 
      RLTISDDSS EKSYSYIED DKNCETHFP DVAIGSFLD 
      TDISLVYCA RLITKSFLL SGQKGVCIP DKSVRVSVK 
      SLALTCLAS IVRIYPKIL LLCLGKKCK SKNHLPIAD 
      LILYENHTD PQLRGVFRT LLSNFITAV LSETHSSYD 
      KWIESNCCD GNFARLQLD SLVKFIIKG LEDESSGCA 
      RQTIISLDV CLEKIAESV NNIHVLPIL NCLPLLATN 
      PYWLVKVAL CELVSKLPY VTICNITGS SDFQNRIID 
      DVLFNFLRN DDARVRSGA ASAIVSVIP FLYHEENAV 
      TAKAIHYKK LFLSNLCDK NTEKDLTKK HLIDNMPSP 
      FSQILHERC DKTEYSLSR IVTKLYHFL LTGTSNHFI 
      YGCLETLSL LSNAYPTTV YTKAWNCKI FQTEKDNSN 
      SDVTLDLFN ICVYLLSHS VICYDLTCH LNLLNLSSN 
      LYAGYALSL IKPISESEP STQPWDMFS SPKFSKLSD 
      HFLSHLVKL LNIFHYVLD DVTPSQLQS KPVLPNLST 
      ASPIKRRKS DLEKKVLSP SKTPEKEDK DKKDSKGAF 
      LHLPHYMKF YEILRQAYT NYKITLESE ACVKFSGLL 
      TQTLHSFAV LMEVGTLVE FHAIAEDIL GYFRFTFTL 
      EASATVECV QQLLKCLFG NNLTSNIGL IREVNQAEV 
      GEQSNGFYH NLYQKPYDG VSECFNSFK NIHKVDCDG 
      DSTLMGYLH RRDAKPLPV RNSDKILAN YIRIFEPMV 
      IKSLKHYTI TSDVRLQCR VLQLLNQLI QLRVNYCLL 
      DSEQIFIGF VLKQFDYIE KGQILLASD LVKKIFQFL 
      VQLSHSKQH SKSIITVPK IIHLCDGLM ASEQSPLTH 
      CIPALEPIV ENIFLTRNK SNTADAKEL ETTRDVVLS 
      MLLRLYEYH EVIDLVALI LDDSKYCSN SEKWLRWST 
      QVTDAILPM LRQNKIRLD SVEALVSLR RLIFVLEPS 
      VFQPVDSVL LLLFQEPLV YDNWQFSYT RRLAKILIG 
      LLVLSPVKE ESLIAKINE LKAEFAPGS IFENCAPTA 
      DPLNVLNSV DIFREVAPE MIFVRFLFR VLNLASRFC 
      VEVTRDGTN DFFIEEISI FLMYCIHIF QSGSHCKIA 
      IFAISNVNV AQLEDISNN FLKVAHEYP IVVFLWSYV 
      LTILNYNEK KFWGQVADL SPVSPSINQ KILSTGSII 
      LFCDYMNQD VLKLDQLSW FLTNYVSQI VHLCDEDPI 
      SEFISSVHR NSATSKIFI DSLMTTNLD SKDPVFVLQ 
      ILKSVENTH PYHTGTLLK LLLPKLLIS KELAVSRLV 
      ANLASRKIE LLLTMSMAE VHEQLAKED LLAIIKKIA 
      ALNLVRKNE ALSSLLNKL CTQYYDISP LEFDQKRSV 
      NPDYIKKLA IDKNWYLAQ IREKCNSKE TAQLLTRLD 
      YEELCNFLR NADFSKVVF KECLRLGLD RNKTVEPLI 
      TATVTCLSE EVTTVCDHI QVFSVPTLE DSERFDSRF 
      LTQIYDLIP SVTFYIDQL PETKVSDNS EVLVKFGVI 
      CLESINYLI DRKNFNLNY IDLAIHCCE SVLKEKKLF 
      GVLNQESHI SWLCSIVNS LYKLVEYLL SDKGQLPAI 
      SSSVLQTTP AGEACYQMY SLVTWLYKN SDPIVPKLL 
      FKPIKNIIV SLSRLSVVN SFVLIPTQV LKSGWTPEL 
      VGTFQTQVP PLPIEYLQE IDVLEEFIF RTTLLGWTS 
      RQQFEETWM SLLSVLCNN FEDKDSLVV QDVVHASSL 
      AVKAITALL VQTLYFPTP GNTNLSQLI HVSRYPNTP 
      KSTISNEKL RKIQDTIER KYREAGYLP KIGNIFEHT 
      NFEKRCKHY SYGQVSVKY LSIAAKIVE IDDKCPTAN 
      IWKHREELL ESHGLDINS CLQFLLDYY TQQLKNQGS 
      TPLRFLHEI VRSTVTISD LFTDKSQFS WMLEVFLEL 
      SKLHTVEDE LLHQYLIVG ISKATAVLT PDLETYETI 
      KKLLTQYLK SSFLPSRMA CLHGLLYLL EGCKLSNIS 
      IGGISEEMQ IILPCATEY VQCNLNSNN EILKRSQEH 
      TILVWTLGF FIVENIEET HLESNFISN VLSTAFTVL 
      LQQKPPNNL QIILMKALE RLVIFKPTL TKGISKQIM 
      SLALEKTKT ENPNVSILG LQLLLSYMY TDCSEQLEA 
      VGSPTNPDH LVQTIEKIS AIFEHIKRG YMSQVEILC 
      HVLPDILND FFSPADILT KVISEFLSP QQPHPQLLS 
      KVVFRVFER AIEEKQLPL LQDWVVFSL SNFTQSLSV 
      GMATWCLTC FFISASSNE WLRLFFPYV QTRVGRYEY 
      EDRKMLCIA GADFYKNLT NQNQKDTFI ENFRKIKEQ 
      PDTLFTDLL SSL
    CDS sequence
    • >TC012221 RA
      atggccacc caggaaaaa ctgctaaaa tcgctggaa 
      gtcctcaag accctctcg gcacctaac caaacattt 
      gactcctca aaaaaaaag gacaagatc cagcactgt 
      cacatcgtc acggaggca atttccaat tcaacgatc 
      aaaatcgcc cccagtttc cccacctac ctcagcata 
      accatcgag gtgcttttg cagttatgc gacgaccag 
      gagtccgac gtgcggatg acagcaggg gactgtctc 
      aataaagtg atcagagcc atgaacgat gggaacatt 
      ggtaaagta caaattgaa ctacataaa gggatcaag 
      caaaacggt tcagctcga tcactgcga gccgccttg 
      aagcggttt tcgcagctg gcacacttg atacggccc 
      cataagggc aagccgtac gtggttaac ctttttccc 
      accttgatt aaggtctcg cagcggcct gaggagcaa 
      attcatgag actttggcc aatagtttg gcgccgata 
      atgcgggtt ttggggagc tttacgtca gaggttgag 
      ataaaagat ttattgaaa ggtttcttg aaaaacatc 
      tcacactct tcgcctgtt atcaggcgg tctgcgacc 
      acttgtgtg ttaaatgtg tgtctgaac tgtagaaaa 
      ccgtctatt tttatggca tacgtaatt aattatttg 
      ttagatttg ttaattcca gtcagtgat aatcaaagt 
      agttttcta atattggga gttttggcg tgtttgaag 
      aatttatta caacacttg aacgataat caaaacgga 
      gataatgct tccttatcc gtggataaa ttgttacaa 
      atttacgag ttgtgtctc cactatttt tctaataag 
      gatcacaac gttgtgaat gcagcttta gaaactttg 
      aatgtttta ctccagaat tgttccctt gaattgagg 
      caaatttta ttgagcgaa agtggcgta gataaaagt 
      aggattagt ttaccaact tcttattca atgtcagca 
      ctgcgttcc ccaagtcag ttaagtgtt gcaacaagt 
      gcagcaaat gacgatttg tttctggag tccgaactt 
      accgatact ataaggtcg gatatagaa aaatggata 
      gatgagtcg aaactttca gtaatgaac gtaccttgt 
      gccaaaact aacgaacta gaaaagtcg ttgtcaagt 
      gagagtaaa aactcctca cttgatgtg tctttggca 
      cagcttgat agcttcgat tataagatt tctgaatac 
      gtaggaaaa agtcacagt gttggtgat aatctggaa 
      cgactcacg ataagtgac gattcctct gaaaaaagc 
      tactcgtat attgaggat gacaagaac tgtgaaaca 
      cattttcca gacgttgct attggtagt tttctagac 
      accgatatt tcgctagtt tattgtgcg agactgatc 
      accaaatcg tttcttttg tcaggccag aagggcgtt 
      tgtattcct gataaatca gtccgagtt tccgtaaaa 
      tccctagct ttgacctgt cttgcctca attgtgcga 
      atctaccca aaaatattg ctcctctgt ttgggtaaa 
      aaatgcaaa tcgaaaaat cacctacca atcgccgac 
      ttgatattg tacgaaaac cacaccgac ccgcaactg 
      cggggcgtt tttaggaca cttttatcc aattttatc 
      acagctgtg ttgagcgaa actcactcg agttacgac 
      aaatggatc gagtcgaat tgttgcgat gggaatttt 
      gcccgactt cagctggat tctctcgtg aaatttata 
      ataaaaggc cttgaggac gaatcgtcc ggttgtgcg 
      cgacaaaca atcataagt ttggatgtt tgtttggaa 
      aaaatcgca gaatcggtt aacaacatt cacgtttta 
      ccgattttg aattgtctg ccactttta gcaaccaat 
      ccgtactgg cttgtgaaa gtcgctttg tgtgaactt 
      gtttcgaag cttccctac gtcactatt tgcaatatc 
      actggttct agtgatttc caaaatcgg ataattgac 
      gatgttttg ttcaatttt ttgcgaaat gatgacgct 
      cgggttagg agtggcgcc gctagtgcc attgtttct 
      gtaattccg tttttgtat cacgaggaa aatgctgtt 
      acggccaaa gcgattcat tacaaaaaa ttgtttttg 
      tcaaacttg tgtgataaa aacacggag aaagactta 
      acaaagaaa caccttatc gataatatg ccgtctccg 
      tttagccaa attttgcac gaacgctgt gataaaacc 
      gaatattct ctatcgaga attgttaca aagttgtat 
      cattttcta ctgaccgga acatcgaat catttcata 
      tatggttgc ttggaaaca ctatcactt ctctcaaac 
      gcatatcct acaacagtc tacactaaa gcctggaac 
      tgtaaaata tttcaaacg gagaaagac aattctaat 
      tcagatgtt acattagac ttatttaac atttgtgtg 
      tatttgctg agccattcc gtgatttgt tacgacttg 
      acgtgccat ttaaacctc ctaaatctc tcgtccaat 
      ctctacgcc gggtacgcc ctcagtctg atcaaaccc 
      atatctgag agtgagccc tcaacccaa ccatgggac 
      atgttctca agccccaaa ttctccaaa ctgagcgac 
      catttcttg tcccatttg gtgaaactc ctcaatatt 
      tttcactac gttcttgac gacgtcacc ccgagtcag 
      ctccaatca aaacctgtc ttacccaac ttgtcgacg 
      gcttcgcca atcaaaagg cgcaaaagt gacttggag 
      aagaaggtt ctaagtccg agtaaaaca ccggagaag 
      gaggataag gacaagaag gactcaaaa ggggcgttt 
      ttgcacctt ccccactac atgaagttt tacgaaatt 
      ctgcgacag gcttatacg aattataag ataacgtta 
      gagagtgaa gcctgtgtg aagttctca gggttgtta 
      acacagacg cttcactcg tttgcggtt ttgatggaa 
      gtggggact ctggttgag tttcacgcg attgccgaa 
      gatattttg gggtatttt cggttcacg ttcacgctg 
      gaggcgtcg gcgacggtt gagtgcgtc cagcagcta 
      ctcaagtgt ttgtttggg aacaacttg acgagtaat 
      attggcctg atcagggaa gtaaaccag gcggaagtg 
      ggcgagcaa tcgaacgga ttttaccac aacttgtac 
      caaaagccg tacgatggt gtgtccgag tgcttcaat 
      tcgttcaaa aacattcac aaagtggac tgtgacggg 
      gacagcact ctcatgggc tacttgcac cggcgggac 
      gccaaaccc ttgcctgtg cgaaactcg gataaaatt 
      ctggccaat tacatccgc atttttgag ccaatggtc 
      atcaagtct ttaaagcat tacactatc acaagcgac 
      gttaggctg cagtgcaga gtgctgcag ttgctcaac 
      cagttgatc cagctcagg gtgaactac tgtctcctc 
      gattcggag caaattttc atcggattt gttctgaag 
      cagttcgac tacatcgaa aaggggcag attctgctt 
      gccagcgat ttggtgaag aaaatcttc caattccta 
      gtccagctt tcccacagt aagcagcac tccaaaagc 
      atcataaca gtcccgaaa ataatccat ttgtgtgac 
      ggtttgatg gccagtgag cagtcgcct ttgacgcac 
      tgcatccca gcgctggag ccaatcgtt gaaaatatt 
      ttcctgaca cgtaacaaa tcaaacaca gccgacgcc 
      aaagaattg gaaacgacg cgagacgtt gtcttgtcg 
      atgctgttg cgactgtat gagtaccac gaggttatc 
      gacttggta gctttgatt ctggacgat agcaaatat 
      tgttccaat tcggaaaag tggctccgg tggtcgacc 
      caagtcacc gatgcgatt ttgccaatg ctgaggcag 
      aataagatt cgactggat agtgtggag gcgttggtt 
      tcgctgcga agactgatt tttgtgctg gaaccgtcg 
      gtgttccag cctgttgac agtgtttta cttttgttg 
      tttcaagaa ccgttggtt tatgataat tggcaattt 
      tcgtatacc cgaagactg gcgaaaatt ctgattggt 
      ttgttggtt ttatcgcca gtgaaggag gaaagtctc 
      attgcgaaa atcaacgaa ttgaaggcg gagttcgca 
      ccagggagt atttttgaa aattgcgca ccgacggct 
      gatcctttg aatgtgcta aacagtgtt gatattttc 
      cgggaggtt gctccggaa atgattttt gttcggttt 
      ttgttccgg gtgttaaac ctcgcatcg aggttttgt 
      gtcgaagtt acgagggat ggaactaat gattttttt 
      attgaggaa atttccatt tttcttatg tattgtatt 
      catattttc cagtcaggg agtcattgt aaaattgca 
      atttttgcg atttctaat gttaatgta gcacagttg 
      gaggatatt agtaataat tttttgaaa gttgcccac 
      gaatatcca attgtagta tttttgtgg tcttacgtt 
      ctcactatt ttgaactac aatgagaag aaattttgg 
      ggtcaagtt gcagatttg agcccggtt tcgccttcc 
      atcaaccag aaaattttg tccaccggc agcattatt 
      ttgttttgt gattatatg aatcaggat gtgctaaaa 
      ttggaccag ttatcctgg tttttgacc aattacgtt 
      tctcaaatc gtccattta tgtgacgag gaccctatt 
      tccgaattt atttcatca gtgcaccgc aacagcgcc 
      accagcaaa atttttata gacagtcta atgaccaca 
      aatttagat tcgaaagac ccggttttt gtgctacag 
      attttaaaa tccgttgaa aacacgcac ccttaccac 
      actggaacc ctcctcaaa cttttacta ccaaaactt 
      ttaatttcg aaagaattg gctgtttct agactggta 
      gcaaattta gcaagtcgt aaaatcgaa ctcctcctc 
      accatgtca atggcggag gtgcacgaa cagttggcc 
      aaagaggat ttgctggca attattaaa aaaatcgca 
      gctttaaat ttagtgaga aaaaacgag gctttatcg 
      agtcttttg aataaattg tgtacacaa tattacgac 
      ataagccca ctggagttt gaccaaaaa cgttcggtc 
      aatcccgac tatatcaaa aaactcgca attgacaaa 
      aactggtat ttggcacaa atccgcgag aaatgtaac 
      agcaaagaa acggcgcag cttttgact cgattggat 
      tacgaagag ttgtgcaat tttttacga aatgcggac 
      ttcagcaaa gttgttttt aaagagtgc ctcaggttg 
      gggttggac agaaataaa acagttgag ccactgatt 
      acagcaacg gttacttgt ttatcggag gaagtgacg 
      acggtctgt gaccatatt caggttttt agtgtgcca 
      acgctcgaa gactccgaa cgttttgat tcgagattt 
      ttgacccaa atttacgac ctgatcccg tctgtaacg 
      ttctacatt gatcagtta cccgaaacc aaagtttcg 
      gacaatagc gaggtcctc gtgaaattt ggtgtaatt 
      tgtttagag tcaataaac tacttaatc gaccgtaaa 
      aattttaat ctaaattac attgacttg gcgatccac 
      tgctgtgag tcggtactt aaggagaaa aaattgttt 
      ggtgtgttg aatcaagag tcccacata tcatggttg 
      tgctccatt gttaatagt ttatataag ttagttgag 
      tatttatta tcagataag ggacagtta cccgcgata 
      tcaagcagt gttttacaa accacccct gcgggtgag 
      gcttgttac caaatgtat agtctagtg acctggttg 
      tacaaaaac tcggaccca atcgttccg aaactttta 
      tttaaaccg atcaaaaat atcatcgtt tcgttgtcg 
      cgcctctcc gttgttaat tccttcgtg ttgataccg 
      acccaagta ttgaaaagt gggtggacc cccgaactc 
      gtcggcacc ttccagacg caggtcccg cccttgccg 
      atcgagtac ctgcaagaa atcgacgtt ctcgaagaa 
      tttatcttc agaacaaca ctcttaggc tggacctct 
      cggcaacaa ttcgaggag acctggatg tctcttttg 
      agcgttctg tgcaacaac ttcgaagac aaggactcg 
      ctagttgtg caagacgtc gtccacgcg tccagttta 
      gcggtcaaa gcaatcacg gcactttta gtacaaact 
      ttgtatttc ccaactcct ggaaacacc aacttatcg 
      caattaatc cacgtttcg cggtatcct aacacaccg 
      aaaagcaca atcagtaac gaaaaattg cggaaaatt 
      caagacaca atcgagcgc aagtatcga gaggcgggt 
      tatcttcca aaaattggg aatattttc gaacacacg 
      aacttcgag aaacggtgc aaacactat tcttacgga 
      caagtgtcg gtgaaatac ctctcaatt gcggcaaaa 
      atcgtcgaa atcgacgac aagtgcccc actgcgaat 
      atctggaaa cacagagag gagttgttg gagagtcac 
      gggttggat attaattcg tgtttgcag tttcttctg 
      gattactac acccaacaa ttgaaaaat caagggtca 
      acaccgctt cgtttcttg cacgaaatc gtcagatct 
      accgtgaca atttcagat ttatttacg gacaagtca 
      cagtttagc tggatgttg gaagtgttt ctagaattg 
      tcaaaattg cacactgtt gaggacgag ttgctccac 
      cagtattta attgtgggg atttcgaaa gccacggct 
      gttttaaca ccggacttg gagacgtac gaaacaata 
      aaaaagttg ttaacgcag tatttgaag agctcgttt 
      ttgccgtcg cgaatggcc tgtttgcac gggcttttg 
      taccttttg gagggttgc aagttgagt aatattagc 
      attgggggg atttcggaa gaaatgcaa attattcta 
      ccctgtgcg acagaatac gtccagtgc aatttaaat 
      tcaaataac gaaatttta aaacgttcg caagaacac 
      acaattcta gtctggact ttgggtttt ttcattgtt 
      gaaaacatc gaagagact catctcgag tcgaatttt 
      atttcaaat gttttgtca actgcgttc acagtgttg 
      ttgcagcaa aagccaccg aataattta caaataatt 
      ttgatgaag gctttggaa cgactggtg atattcaag 
      ccaaccctc acaaaaggc ataagtaaa caaataatg 
      agcctagca ctcgaaaaa acaaaaacc gaaaatccg 
      aacgtatca atcctgggc ctccaactc ctcctcagc 
      tacatgtac accgactgt tccgagcag ctcgaagct 
      gtgggcagc cccacaaac cccgaccac ttagtacaa 
      acaatcgaa aaaatctct gcaattttc gagcacatc 
      aaacgtggc tacatgtcc caagttgaa attttgtgc 
      cacgttctg cccgacata ctcaacgac tttttctcc 
      ccggccgac atcctcacg aaggtgata agcgagttc 
      ctatcgccg cagcagccc catccccag cttttgagc 
      aaagttgtc tttcgggtg ttcgagcga gcgattgag 
      gagaaacag cttccgtta ctacaagac tgggtggtt 
      tttagtctt tccaatttc acgcaatcg ttgtcggtt 
      ggaatggca acgtggtgc ttgacttgc ttcttcata 
      agcgcaagt tcgaacgag tggttgcgg ctatttttc 
      ccttacgtg cagacgcgt gtcggtagg tacgagtac 
      gaggaccgg aaaatgctg tgcattgct ggggcggac 
      ttctacaag aacctgacc aaccagaac cagaaagac 
      acgttcatt gagaatttt cgcaaaatt aaggagcaa 
      cccgacacg ctgttcacc gatttgtta tcgtctctg 
      taa
    mRNA sequence
    • >TC012221 RA
      tctaccacc ccaaagaaa aaaagcagc ggatcattc 
      aaatttctt ctaaataaa cacaaacac gcgaaagct 
      gtgacttta ccacaaaat cggccgatt ttaaagata 
      ttgttgcaa actgaatgc cacaattat taaaatttg 
      gacaattct tggcaaaat aaaatttga catttgttt 
      tgacagtcg acttgactt tcccgtatg tttttcgtg 
      tgatgtgtc taaaaaatg ttaaaataa acaatgcgt 
      ttaaaactg gtggacagc agctaacag ccgattaaa 
      accgtcttg aatcacccc ctttagcta aaaatggcc 
      acccaggaa aaactgcta aaatcgctg gaagtcctc 
      aagaccctc tcggcacct aaccaaaca tttgactcc 
      tcaaaaaaa aaggacaag atccagcac tgtcacatc 
      gtcacggag gcaatttcc aattcaacg atcaaaatc 
      gcccccagt ttccccacc tacctcagc ataaccatc 
      gaggtgctt ttgcagtta tgcgacgac caggagtcc 
      gacgtgcgg atgacagca ggggactgt ctcaataaa 
      gtgatcaga gccatgaac gatgggaac attggtaaa 
      gtacaaatt gaactacat aaagggatc aagcaaaac 
      ggttcagct cgatcactg cgagccgcc ttgaagcgg 
      ttttcgcag ctggcacac ttgatacgg ccccataag 
      ggcaagccg tacgtggtt aaccttttt cccaccttg 
      attaaggtc tcgcagcgg cctgaggag caaattcat 
      gagactttg gccaatagt ttggcgccg ataatgcgg 
      gttttgggg agctttacg tcagaggtt gagataaaa 
      gatttattg aaaggtttc ttgaaaaac atctcacac 
      tcttcgcct gttatcagg cggtctgcg accacttgt 
      gtgttaaat gtgtgtctg aactgtaga aaaccgtct 
      atttttatg gcatacgta attaattat ttgttagat 
      ttgttaatt ccagtcagt gataatcaa agtagtttt 
      ctaatattg ggagttttg gcgtgtttg aagaattta 
      ttacaacac ttgaacgat aatcaaaac ggagataat 
      gcttcctta tccgtggat aaattgtta caaatttac 
      gagttgtgt ctccactat ttttctaat aaggatcac 
      aacgttgtg aatgcagct ttagaaact ttgaatgtt 
      ttactccag aattgttcc cttgaattg aggcaaatt 
      ttattgagc gaaagtggc gtagataaa agtaggatt 
      agtttacca acttcttat tcaatgtca gcactgcgt 
      tccccaagt cagttaagt gttgcaaca agtgcagca 
      aatgacgat ttgtttctg gagtccgaa cttaccgat 
      actataagg tcggatata gaaaaatgg atagatgag 
      tcgaaactt tcagtaatg aacgtacct tgtgccaaa 
      actaacgaa ctagaaaag tcgttgtca agtgagagt 
      aaaaactcc tcacttgat gtgtctttg gcacagctt 
      gatagcttc gattataag atttctgaa tacgtagga 
      aaaagtcac agtgttggt gataatctg gaacgactc 
      acgataagt gacgattcc tctgaaaaa agctactcg 
      tatattgag gatgacaag aactgtgaa acacatttt 
      ccagacgtt gctattggt agttttcta gacaccgat 
      atttcgcta gtttattgt gcgagactg atcaccaaa 
      tcgtttctt ttgtcaggc cagaagggc gtttgtatt 
      cctgataaa tcagtccga gtttccgta aaatcccta 
      gctttgacc tgtcttgcc tcaattgtg cgaatctac 
      ccaaaaata ttgctcctc tgtttgggt aaaaaatgc 
      aaatcgaaa aatcaccta ccaatcgcc gacttgata 
      ttgtacgaa aaccacacc gacccgcaa ctgcggggc 
      gtttttagg acactttta tccaatttt atcacagct 
      gtgttgagc gaaactcac tcgagttac gacaaatgg 
      atcgagtcg aattgttgc gatgggaat tttgcccga 
      cttcagctg gattctctc gtgaaattt ataataaaa 
      ggccttgag gacgaatcg tccggttgt gcgcgacaa 
      acaatcata agtttggat gtttgtttg gaaaaaatc 
      gcagaatcg gttaacaac attcacgtt ttaccgatt 
      ttgaattgt ctgccactt ttagcaacc aatccgtac 
      tggcttgtg aaagtcgct ttgtgtgaa cttgtttcg 
      aagcttccc tacgtcact atttgcaat atcactggt 
      tctagtgat ttccaaaat cggataatt gacgatgtt 
      ttgttcaat tttttgcga aatgatgac gctcgggtt 
      aggagtggc gccgctagt gccattgtt tctgtaatt 
      ccgtttttg tatcacgag gaaaatgct gttacggcc 
      aaagcgatt cattacaaa aaattgttt ttgtcaaac 
      ttgtgtgat aaaaacacg gagaaagac ttaacaaag 
      aaacacctt atcgataat atgccgtct ccgtttagc 
      caaattttg cacgaacgc tgtgataaa accgaatat 
      tctctatcg agaattgtt acaaagttg tatcatttt 
      ctactgacc ggaacatcg aatcatttc atatatggt 
      tgcttggaa acactatca cttctctca aacgcatat 
      cctacaaca gtctacact aaagcctgg aactgtaaa 
      atatttcaa acggagaaa gacaattct aattcagat 
      gttacatta gacttattt aacatttgt gtgtatttg 
      ctgagccat tccgtgatt tgttacgac ttgacgtgc 
      catttaaac ctcctaaat ctctcgtcc aatctctac 
      gccgggtac gccctcagt ctgatcaaa cccatatct 
      gagagtgag ccctcaacc caaccatgg gacatgttc 
      tcaagcccc aaattctcc aaactgagc gaccatttc 
      ttgtcccat ttggtgaaa ctcctcaat atttttcac 
      tacgttctt gacgacgtc accccgagt cagctccaa 
      tcaaaacct gtcttaccc aacttgtcg acggcttcg 
      ccaatcaaa aggcgcaaa agtgacttg gagaagaag 
      gttctaagt ccgagtaaa acaccggag aaggaggat 
      aaggacaag aaggactca aaaggggcg tttttgcac 
      cttccccac tacatgaag ttttacgaa attctgcga 
      caggcttat acgaattat aagataacg ttagagagt 
      gaagcctgt gtgaagttc tcagggttg ttaacacag 
      acgcttcac tcgtttgcg gttttgatg gaagtgggg 
      actctggtt gagtttcac gcgattgcc gaagatatt 
      ttggggtat tttcggttc acgttcacg ctggaggcg 
      tcggcgacg gttgagtgc gtccagcag ctactcaag 
      tgtttgttt gggaacaac ttgacgagt aatattggc 
      ctgatcagg gaagtaaac caggcggaa gtgggcgag 
      caatcgaac ggattttac cacaacttg taccaaaag 
      ccgtacgat ggtgtgtcc gagtgcttc aattcgttc 
      aaaaacatt cacaaagtg gactgtgac ggggacagc 
      actctcatg ggctacttg caccggcgg gacgccaaa 
      cccttgcct gtgcgaaac tcggataaa attctggcc 
      aattacatc cgcattttt gagccaatg gtcatcaag 
      tctttaaag cattacact atcacaagc gacgttagg 
      ctgcagtgc agagtgctg cagttgctc aaccagttg 
      atccagctc agggtgaac tactgtctc ctcgattcg 
      gagcaaatt ttcatcgga tttgttctg aagcagttc 
      gactacatc gaaaagggg cagattctg cttgccagc 
      gatttggtg aagaaaatc ttccaattc ctagtccag 
      ctttcccac agtaagcag cactccaaa agcatcata 
      acagtcccg aaaataatc catttgtgt gacggtttg 
      atggccagt gagcagtcg cctttgacg cactgcatc 
      ccagcgctg gagccaatc gttgaaaat attttcctg 
      acacgtaac aaatcaaac acagccgac gccaaagaa 
      ttggaaacg acgcgagac gttgtcttg tcgatgctg 
      ttgcgactg tatgagtac cacgaggtt atcgacttg 
      gtagctttg attctggac gatagcaaa tattgttcc 
      aattcggaa aagtggctc cggtggtcg acccaagtc 
      accgatgcg attttgcca atgctgagg cagaataag 
      attcgactg gatagtgtg gaggcgttg gtttcgctg 
      cgaagactg atttttgtg ctggaaccg tcggtgttc 
      cagcctgtt gacagtgtt ttacttttg ttgtttcaa 
      gaaccgttg gtttatgat aattggcaa ttttcgtat 
      acccgaaga ctggcgaaa attctgatt ggtttgttg 
      gttttatcg ccagtgaag gaggaaagt ctcattgcg 
      aaaatcaac gaattgaag gcggagttc gcaccaggg 
      agtattttt gaaaattgc gcaccgacg gctgatcct 
      ttgaatgtg ctaaacagt gttgatatt ttccgggag 
      gttgctccg gaaatgatt tttgttcgg tttttgttc 
      cgggtgtta aacctcgca tcgaggttt tgtgtcgaa 
      gttacgagg gatggaact aatgatttt tttattgag 
      gaaatttcc atttttctt atgtattgt attcatatt 
      ttccagtca gggagtcat tgtaaaatt gcaattttt 
      gcgatttct aatgttaat gtagcacag ttggaggat 
      attagtaat aattttttg aaagttgcc cacgaatat 
      ccaattgta gtatttttg tggtcttac gttctcact 
      attttgaac tacaatgag aagaaattt tggggtcaa 
      gttgcagat ttgagcccg gtttcgcct tccatcaac 
      cagaaaatt ttgtccacc ggcagcatt attttgttt 
      tgtgattat atgaatcag gatgtgcta aaattggac 
      cagttatcc tggtttttg accaattac gtttctcaa 
      atcgtccat ttatgtgac gaggaccct atttccgaa 
      tttatttca tcagtgcac cgcaacagc gccaccagc 
      aaaattttt atagacagt ctaatgacc acaaattta 
      gattcgaaa gacccggtt tttgtgcta cagatttta 
      aaatccgtt gaaaacacg cacccttac cacactgga 
      accctcctc aaactttta ctaccaaaa cttttaatt 
      tcgaaagaa ttggctgtt tctagactg gtagcaaat 
      ttagcaagt cgtaaaatc gaactcctc ctcaccatg 
      tcaatggcg gaggtgcac gaacagttg gccaaagag 
      gatttgctg gcaattatt aaaaaaatc gcagcttta 
      aatttagtg agaaaaaac gaggcttta tcgagtctt 
      ttgaataaa ttgtgtaca caatattac gacataagc 
      ccactggag tttgaccaa aaacgttcg gtcaatccc 
      gactatatc aaaaaactc gcaattgac aaaaactgg 
      tatttggca caaatccgc gagaaatgt aacagcaaa 
      gaaacggcg cagcttttg actcgattg gattacgaa 
      gagttgtgc aatttttta cgaaatgcg gacttcagc 
      aaagttgtt tttaaagag tgcctcagg ttggggttg 
      gacagaaat aaaacagtt gagccactg attacagca 
      acggttact tgtttatcg gaggaagtg acgacggtc 
      tgtgaccat attcaggtt tttagtgtg ccaacgctc 
      gaagactcc gaacgtttt gattcgaga tttttgacc 
      caaatttac gacctgatc ccgtctgta acgttctac 
      attgatcag ttacccgaa accaaagtt tcggacaat 
      agcgaggtc ctcgtgaaa tttggtgta atttgttta 
      gagtcaata aactactta atcgaccgt aaaaatttt 
      aatctaaat tacattgac ttggcgatc cactgctgt 
      gagtcggta cttaaggag aaaaaattg tttggtgtg 
      ttgaatcaa gagtcccac atatcatgg ttgtgctcc 
      attgttaat agtttatat aagttagtt gagtattta 
      ttatcagat aagggacag ttacccgcg atatcaagc 
      agtgtttta caaaccacc cctgcgggt gaggcttgt 
      taccaaatg tatagtcta gtgacctgg ttgtacaaa 
      aactcggac ccaatcgtt ccgaaactt ttatttaaa 
      ccgatcaaa aatatcatc gtttcgttg tcgcgcctc 
      tccgttgtt aattccttc gtgttgata ccgacccaa 
      gtattgaaa agtgggtgg acccccgaa ctcgtcggc 
      accttccag acgcaggtc ccgcccttg ccgatcgag 
      tacctgcaa gaaatcgac gttctcgaa gaatttatc 
      ttcagaaca acactctta ggctggacc tctcggcaa 
      caattcgag gagacctgg atgtctctt ttgagcgtt 
      ctgtgcaac aacttcgaa gacaaggac tcgctagtt 
      gtgcaagac gtcgtccac gcgtccagt ttagcggtc 
      aaagcaatc acggcactt ttagtacaa actttgtat 
      ttcccaact cctggaaac accaactta tcgcaatta 
      atccacgtt tcgcggtat cctaacaca ccgaaaagc 
      acaatcagt aacgaaaaa ttgcggaaa attcaagac 
      acaatcgag cgcaagtat cgagaggcg ggttatctt 
      ccaaaaatt gggaatatt ttcgaacac acgaacttc 
      gagaaacgg tgcaaacac tattcttac ggacaagtg 
      tcggtgaaa tacctctca attgcggca aaaatcgtc 
      gaaatcgac gacaagtgc cccactgcg aatatctgg 
      aaacacaga gaggagttg ttggagagt cacgggttg 
      gatattaat tcgtgtttg cagtttctt ctggattac 
      tacacccaa caattgaaa aatcaaggg tcaacaccg 
      cttcgtttc ttgcacgaa atcgtcaga tctaccgtg 
      acaatttca gatttattt acggacaag tcacagttt 
      agctggatg ttggaagtg tttctagaa ttgtcaaaa 
      ttgcacact gttgaggac gagttgctc caccagtat 
      ttaattgtg gggatttcg aaagccacg gctgtttta 
      acaccggac ttggagacg tacgaaaca ataaaaaag 
      ttgttaacg cagtatttg aagagctcg tttttgccg 
      tcgcgaatg gcctgtttg cacgggctt ttgtacctt 
      ttggagggt tgcaagttg agtaatatt agcattggg 
      gggatttcg gaagaaatg caaattatt ctaccctgt 
      gcgacagaa tacgtccag tgcaattta aattcaaat 
      aacgaaatt ttaaaacgt tcgcaagaa cacacaatt 
      ctagtctgg actttgggt tttttcatt gttgaaaac 
      atcgaagag actcatctc gagtcgaat tttatttca 
      aatgttttg tcaactgcg ttcacagtg ttgttgcag 
      caaaagcca ccgaataat ttacaaata attttgatg 
      aaggctttg gaacgactg gtgatattc aagccaacc 
      ctcacaaaa ggcataagt aaacaaata atgagccta 
      gcactcgaa aaaacaaaa accgaaaat ccgaacgta 
      tcaatcctg ggcctccaa ctcctcctc agctacatg 
      tacaccgac tgttccgag cagctcgaa gctgtgggc 
      agccccaca aaccccgac cacttagta caaacaatc 
      gaaaaaatc tctgcaatt ttcgagcac atcaaacgt 
      ggctacatg tcccaagtt gaaattttg tgccacgtt 
      ctgcccgac atactcaac gactttttc tccccggcc 
      gacatcctc acgaaggtg ataagcgag ttcctatcg 
      ccgcagcag ccccatccc cagcttttg agcaaagtt 
      gtctttcgg gtgttcgag cgagcgatt gaggagaaa 
      cagcttccg ttactacaa gactgggtg gtttttagt 
      ctttccaat ttcacgcaa tcgttgtcg gttggaatg 
      gcaacgtgg tgcttgact tgcttcttc ataagcgca 
      agttcgaac gagtggttg cggctattt ttcccttac 
      gtgcagacg cgtgtcggt aggtacgag tacgaggac 
      cggaaaatg ctgtgcatt gctggggcg gacttctac 
      aagaacctg accaaccag aaccagaaa gacacgttc 
      attgagaat tttcgcaaa attaaggag caacccgac 
      acgctgttc accgatttg ttatcgtct ctgtaaata 
      gtaggactt acaacaaat ttaattatc tctattgag 
      taagaaata aaaagaaat taacatt
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