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Gene information
General information
TC012229 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 30349..31994
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012229
    iBeetle screen iB_09434

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012229 RA
      MGFFKGGKY SYVAAASVN ILAFIVGTA SSWSSPVLP 
      KLQQHLDET PLGRLISPD EASWIGSLL SMGGIVAPL 
      LWGSLVWRV GRKTVAVTV AVPFLVAFL VAAFAQTIA 
      LFYLARVLM GVGIGGMFC VAIIYVVEI AEDANRGLL 
      TASVGFFIV VGLLFPYCV GPFVSIMTF NLILASITL 
      FYIVLFWYI APETPYWLV SVNQDREAL KSLYYLRRR 
      PLKQLEEEL NQIKAYLQT MTHGSFLGI FKTRASTKA 
      LIFSIALTT FQQFSGINV IFSYMQSIF DSTGSDIPA 
      EISSIIVAA VQMIFSTIS PLLSDKAGR RTLLLISIT 
      GAALSEIVL GAYFYMQNS GQDVSDIGW LPVVTLVVF 
      MMFYNCGMG SLPWALMSE LLPSNVISK ATLLITCIY 
      WFVGWVLTQ YFAALNEAV GSAGSFWLF SGFCILFDL 
      FVYFFIFET KGKSLQEIN EILSR
    CDS sequence
    • >TC012229 RA
      atgggtttc ttcaaaggg ggcaagtac tcatacgtg 
      gctgcagct tccgtcaac atcttggcc ttcattgtt 
      ggaacggcc tcgtcgtgg tcgtcccct gtgctacca 
      aaactccag caacacctc gacgaaacg ccacttggg 
      cgcctgatc tcgccggac gaagcctcc tggattggg 
      tccttgctc tcgatgggc ggcatcgtg gcccccttg 
      ctctggggc agcctcgtg tggcgggtt ggccggaaa 
      acggtcgct gtgacagtg gcagtgccg ttcctggtg 
      gcctttcta gtggcagcg ttcgcccaa accatcgcc 
      ctgttctac ctcgcccgc gtcctcatg ggcgtggga 
      atcggcgga atgttctgt gtcgcgatt atttacgtt 
      gttgagatc gccgaagac gcaaatcgg gggctgctg 
      acggcgtct gtggggttt tttatcgtg gtggggctg 
      ctgttcccg tactgcgtt ggcccgttt gtttccatc 
      atgactttt aacttaatt ctcgcgtct atcacgctt 
      ttttacatc gtgttgttc tggtacatc gcccccgaa 
      accccctac tggcttgtt agcgtaaac caagacagg 
      gaagctttg aaatcgttg tattatctc aggcgccgt 
      ccgcttaaa caactcgag gaggaacta aaccagatc 
      aaagcttat ctgcaaacg atgacacac ggttctttt 
      ttgggaatt ttcaaaaca cgggcaagc accaaagct 
      ctgatcttc agcattgct ctgacaact ttccagcag 
      tttagtgga atcaacgtg attttctcc tacatgcag 
      agtattttt gactcgact gggagcgac atcccggcg 
      gagattagc tcgattatt gtcgcagca gtgcagatg 
      attttttcg accatttct cccctgctt agtgacaag 
      gcggggcgc aggactttg ctcctgata agcataaca 
      ggggcggcg ctgtcggaa atcgtccta ggggcctat 
      ttctacatg caaaatagc gggcaggac gtttcggac 
      attggctgg ttgccggtt gtcactctc gtggttttt 
      atgatgttt tataattgt gggatgggc tcgctgcct 
      tgggccctc atgtccgag cttttgccc tcgaacgtg 
      atctcgaaa gccactttg ttgataacg tgtatttat 
      tggtttgtt ggctgggtc ctgacccag tatttcgcg 
      gctttgaac gaagcggtc ggaagtgcg ggctcgttt 
      tggctgttt tcggggttc tgtattttg tttgacttg 
      tttgtgtac ttttttatt tttgagacg aagggcaaa 
      agtttgcag gaaattaat gagattttg agcaggtag
    mRNA sequence
    • >TC012229 RA
      aatcccagc cagtgctcc agcatgggt ttcttcaaa 
      gggggcaag tactcatac gtggctgca gcttccgtc 
      aacatcttg gccttcatt gttggaacg gcctcgtcg 
      tggtcgtcc cctgtgcta ccaaaactc cagcaacac 
      ctcgacgaa acgccactt gggcgcctg atctcgccg 
      gacgaagcc tcctggatt gggtccttg ctctcgatg 
      ggcggcatc gtggccccc ttgctctgg ggcagcctc 
      gtgtggcgg gttggccgg aaaacggtc gctgtgaca 
      gtggcagtg ccgttcctg gtggccttt ctagtggca 
      gcgttcgcc caaaccatc gccctgttc tacctcgcc 
      cgcgtcctc atgggcgtg ggaatcggc ggaatgttc 
      tgtgtcgcg attatttac gttgttgag atcgccgaa 
      gacgcaaat cgggggctg ctgacggcg tctgtgggg 
      ttttttatc gtggtgggg ctgctgttc ccgtactgc 
      gttggcccg tttgtttcc atcatgact tttaactta 
      attctcgcg tctatcacg cttttttac atcgtgttg 
      ttctggtac atcgccccc gaaaccccc tactggctt 
      gttagcgta aaccaagac agggaagct ttgaaatcg 
      ttgtattat ctcaggcgc cgtccgctt aaacaactc 
      gaggaggaa ctaaaccag atcaaagct tatctgcaa 
      acgatgaca cacggttct tttttggga attttcaaa 
      acacgggca agcaccaaa gctctgatc ttcagcatt 
      gctctgaca actttccag cagtttagt ggaatcaac 
      gtgattttc tcctacatg cagagtatt tttgactcg 
      actgggagc gacatcccg gcggagatt agctcgatt 
      attgtcgca gcagtgcag atgattttt tcgaccatt 
      tctcccctg cttagtgac aaggcgggg cgcaggact 
      ttgctcctg ataagcata acaggggcg gcgctgtcg 
      gaaatcgtc ctaggggcc tatttctac atgcaaaat 
      agcgggcag gacgtttcg gacattggc tggttgccg 
      gttgtcact ctcgtggtt tttatgatg ttttataat 
      tgtgggatg ggctcgctg ccttgggcc ctcatgtcc 
      gagcttttg ccctcgaac gtgatctcg aaagccact 
      ttgttgata acgtgtatt tattggttt gttggctgg 
      gtcctgacc cagtatttc gcggctttg aacgaagcg 
      gtcggaagt gcgggctcg ttttggctg ttttcgggg 
      ttctgtatt ttgtttgac ttgtttgtg tactttttt 
      atttttgag acgaagggc aaaagtttg caggaaatt 
      aatgagatt ttgagcagg tagttgaaa atctgtata 
      ggaatttga gatactgcc attgttttt gtcaagtca 
      agcggaaaa atccgaaaa attggtcaa aacgaacca 
      aaaaccata agttttaaa attaataaa attcaggaa 
      atgaaaaat taaaataaa tttgtgata atgtattgt 
      aaatattta tttgtcata atttctatt ttgctaata 
      aattttgca aaaatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY