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Gene information
General information
TC001459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3876578..3879352
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001459
    iBeetle screen iB_09432

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule mostlynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    mandible not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001459 RA
      MSRVAHIST TRKRSRRGQ KTPEATITS HDAPDFSFD 
      SDSENNPIN QQKKRVASL DDFHRKKKL KETVPEENI 
      DEECDIVDE HCSAISISK NKRIILNAE QQKDRRLKL 
      KQYLQLFVD ISKTYLQKI DKGSQRTSR ESGSRVNAA 
      SNKCNPNAL KYFQGTLRP YQVDGVVWL STLFENSIN 
      GILGDDMGL GKTIQVIAL FCYLYERKI PGPFLIVVP 
      LSTLGNWVS EFKKFAPKI PCTTFEFNW TKTEQTRFI 
      NKKYELDGK LVKPVIICT YQAPIQSNC YLRDYEWQY 
      IVVDEGQRL KNPNSKLSL ELRGFLTKN KLLLTGTPL 
      QNEIGELWA LFGFLMPEL FENMEDFST LFDLEDLRD 
      TKKLLETEA ETKMISKIH KVLTLFMLR REKKLVLHD 
      IVPKKEMII YCPLTPVQK YLYRAILAD DTDALNMLK 
      STKLTDELP GTPREKRRL SADTVIFMD RDWNPMVDL 
      QAQDRCHRI GQTKPVMVY SLVTKGTID EIILNRADV 
      KKRLGKVVI KEGQFKGKQ LNDEEIKSL RDLLKEEET 
      TAKINPNGF HYTEQELDQ ILDRSDITR EMEEKICH
    CDS sequence
    • >TC001459 RA
      atgagtcgc gtagctcat atttccaca accagaaaa 
      cgttcaaga agggggcaa aagaccccc gaagccact 
      attactagt catgatgca cctgatttc tcctttgac 
      tctgattcg gagaacaac cccatcaat cagcagaaa 
      aaacgggtc gcctctttg gatgatttt caccggaag 
      aaaaaacta aaagaaacg gtccccgag gaaaatatc 
      gatgaagaa tgtgatata gtagatgag cattgctcg 
      gcgatttca ataagcaaa aacaaaaga attatacta 
      aatgctgag cagcaaaaa gatagacgt cttaaactg 
      aaacagtat ttgcaactt tttgttgac atttcgaag 
      acttactta cagaaaatt gacaagggc agccagagg 
      actagtcga gaatctggt tcaagagtt aacgcggct 
      tcaaataag tgtaatccc aacgctctg aaatatttc 
      caggggact ttacgcccc tatcaagtt gatggggtc 
      gtctggtta tctaccctt tttgaaaac tccatcaat 
      ggtatcttg ggcgatgac atgggcctc ggtaaaacc 
      atccaagtc atcgctctg ttttgctat ttgtacgaa 
      cgtaaaata cctgggccg ttcctaatt gttgtcccg 
      ctttcaacc cttggaaac tgggtctct gaatttaag 
      aaattcgct ccgaaaata ccatgcacc acgtttgag 
      tttaactgg accaaaacg gaacaaacc cgattcata 
      aacaaaaaa tacgaactg gacggtaaa ctggttaag 
      cccgtcatt atctgcacc taccaagcc cccattcag 
      tcaaactgt tatttgcgg gactacgaa tggcagtac 
      attgtcgtc gacgagggg cagcgcctc aaaaacccc 
      aattcgaag ctgtctctc gaactgcgc ggtttccta 
      actaaaaac aaacttctc ttaactgga accccgcta 
      caaaacgaa atcggcgaa ctgtgggct ttattcggc 
      ttcttgatg cccgaactg ttcgaaaat atggaggac 
      ttttccaca cttttcgac ctcgaggat ctcagggac 
      accaaaaag ttgctagag accgaagct gagaccaaa 
      atgatttcg aaaatccac aaagttttg actctcttc 
      atgttgcga cgcgagaag aagctcgtt ctgcatgac 
      atcgtccca aagaaagaa atgatcatt tactgccct 
      ttaacacct gtacaaaag tacctctat cgcgctatt 
      ttagccgac gacacggat gcgctaaat atgctcaaa 
      agcaccaaa ctgacggac gagctccca gggacgccc 
      agagagaag agacggtta tccgccgat actgtcatt 
      ttcatggat agggattgg aaccccatg gtcgaccta 
      caggcccaa gacaggtgt cacaggatc ggtcaaacc 
      aaacctgtg atggtctac agtttggtc accaaggga 
      acaatcgac gaaattatc ctaaatcgt gcggacgta 
      aaaaagaga ctaggaaaa gttgtcata aaagaaggg 
      caatttaaa ggcaagcag ctaaatgat gaggagata 
      aaatcatta agagatcta ttgaaggag gaagaaacc 
      actgccaaa ataaatcct aacggattt cactacaca 
      gaacaagaa cttgaccaa attctggac cgaagtgat 
      attaccagg gaaatggag gaaaaaatt tgtcattag
    mRNA sequence
    • >TC001459 RA
      tgctccgcc atgcaagtg tgccggtta ttagtatga 
      gtaaaagaa gtggttgtt caccggatt tattgcttg 
      attttcttt taaacctat tgcactttt aattgtgtt 
      tcgtagttc tcgttaagt gttaagtgt ttagtgttt 
      gcgttcgtc ataacaatc agtgtttag tgtttgcgt 
      tcgtcataa caatcagtt aacatgagt cgcgtagct 
      catatttcc acaaccaga aaacgttca agaaggggg 
      caaaagacc cccgaagcc actattact agtcatgat 
      gcacctgat ttctccttt gactctgat tcggagaac 
      aaccccatc aatcagcag aaaaaacgg gtcgcctct 
      ttggatgat tttcaccgg aagaaaaaa ctaaaagaa 
      acggtcccc gaggaaaat atcgatgaa gaatgtgat 
      atagtagat gagcattgc tcggcgatt tcaataagc 
      aaaaacaaa agaattata ctaaatgct gagcagcaa 
      aaagataga cgtcttaaa ctgaaacag tatttgcaa 
      ctttttgtt gacatttcg aagacttac ttacagaaa 
      attgacaag ggcagccag aggactagt cgagaatct 
      ggttcaaga gttaacgcg gcttcaaat aagtgtaat 
      cccaacgct ctgaaatat ttccagggg actttacgc 
      ccctatcaa gttgatggg gtcgtctgg ttatctacc 
      ctttttgaa aactccatc aatggtatc ttgggcgat 
      gacatgggc ctcggtaaa accatccaa gtcatcgct 
      ctgttttgc tatttgtac gaacgtaaa atacctggg 
      ccgttccta attgttgtc ccgctttca acccttgga 
      aactgggtc tctgaattt aagaaattc gctccgaaa 
      ataccatgc accacgttt gagtttaac tggaccaaa 
      acggaacaa acccgattc ataaacaaa aaatacgaa 
      ctggacggt aaactggtt aagcccgtc attatctgc 
      acctaccaa gcccccatt cagtcaaac tgttatttg 
      cgggactac gaatggcag tacattgtc gtcgacgag 
      gggcagcgc ctcaaaaac cccaattcg aagctgtct 
      ctcgaactg cgcggtttc ctaactaaa aacaaactt 
      ctcttaact ggaaccccg ctacaaaac gaaatcggc 
      gaactgtgg gctttattc ggcttcttg atgcccgaa 
      ctgttcgaa aatatggag gacttttcc acacttttc 
      gacctcgag gatctcagg gacaccaaa aagttgcta 
      gagaccgaa gctgagacc aaaatgatt tcgaaaatc 
      cacaaagtt ttgactctc ttcatgttg cgacgcgag 
      aagaagctc gttctgcat gacatcgtc ccaaagaaa 
      gaaatgatc atttactgc cctttaaca cctgtacaa 
      aagtacctc tatcgcgct attttagcc gacgacacg 
      gatgcgcta aatatgctc aaaagcacc aaactgacg 
      gacgagctc ccagggacg cccagagag aagagacgg 
      ttatccgcc gatactgtc attttcatg gatagggat 
      tggaacccc atggtcgac ctacaggcc caagacagg 
      tgtcacagg atcggtcaa accaaacct gtgatggtc 
      tacagtttg gtcaccaag ggaacaatc gacgaaatt 
      atcctaaat cgtgcggac gtaaaaaag agactagga 
      aaagttgtc ataaaagaa gggcaattt aaaggcaag 
      cagctaaat gatgaggag ataaaatca ttaagagat 
      ctattgaag gaggaagaa accactgcc aaaataaat 
      cctaacgga tttcactac acagaacaa gaacttgac 
      caaattctg gaccgaagt gatattacc agggaaatg 
      gaggaaaaa atttgtcat tagccccca aggggttac 
      tactcgtta atgtgttat cag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY