Status
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Gene information
General information
TC001449 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3756001..3765627
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001449
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09431

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001449 RA
      MVPHGPLNF LLCLSIFLT LTLSAEHDF RYISFQDLQ 
      QSLNKFSQD EVVSYSQML FDVARNQVL VGARDVLFR 
      LSLTKLELL ESAEWPAST DKRIECINK GQSDEFCHN 
      YIKILLTNG KNLFVCGTN AFSPQCSTR KIENVSDVT 
      EWVDGLAKC PYNPAANIT GFMSERGEY YFGGPTDFS 
      SSDFVFSKN VGTVTIRTK QYNSFLLND PQFVGTFET 
      ENFVYFLFR ETAVEYMNC GKTIYSRIA RVCKNDPGG 
      HTMLKDNWT TFLKARLNC SVSGEYPFY FDEIQSMAY 
      VPDENMVYA TFTTPSNSI AGSAICAFN LSSIEAAFA 
      GPFKYRENM DSAWGQYNV HHRDHFNCK SSPRSSHLL 
      ETSKYQLMD SAVQATTLN PLHVAQLER FTHITIDVL 
      STKIHTSVH VIYVSTTTG VIKKISVLP RTQETCVVE 
      IWQTVQNPA TPVKSIQFL KATKSIYVA TEHQVMKIP 
      AEHCSRHTS KTSCLNAMD PYCGWNERY ENCSTAPNA 
      DPLDKYWKQ AVTTCPILD TVVDGGWSS WSPWAPCVQ 
      RSAPESDAD TCLCQVREC NNPAPKNGG LPCGGPAIA 
      VTNCTVHGG WSVWSAWSA CSATCGTAV KTRSRTCTN 
      PAPAHGGRV CVGQDRTEA FCDAEIPPC PAMPQDGGW 
      GEWSDWGDC SPSHCSGCK RRTRVCNNP PPENGGQFC 
      AGNDEEFKF ECPEHRKTV STPWISLTN SSGDYNKRF 
      RCTCKAPVK MSLIKVAMK EEVCNNDNC FTKDSEHPK 
      WSAWGPWGE CSVQCGGGL QNRTRYCEG IGCTGPSVQ 
      TRECNKHHC REWGCWSEW SPCNASCGW GVKTRHRVC 
      LGSNCSGHD WEKEPCQNA PCESESLHY NAPSGQVEE 
      TQCFNVEQS FK
    CDS sequence
    • >TC001449 RA
      atggtgcca catggacct ttaaatttt ttactttgc 
      ctgtcgatt ttcctaaca ctaactcta tcggcggag 
      catgatttt cgatatatt tcgttccaa gatcttcag 
      caatcttta aacaaattt tcacaagat gaggttgtc 
      tcctactcg cagatgctt ttcgacgtg gccaggaac 
      caagtatta gtcggcgca agagacgtc ctctttcgc 
      ttatctttg acgaaactg gagcttttg gagtcggcc 
      gaatggccc gcctccaca gacaaacgc atcgaatgc 
      atcaacaaa ggccaatcg gacgagttt tgccacaac 
      tacatcaaa attttgcta accaatggc aaaaaccta 
      ttcgtctgt ggcacgaac gcattctcg cctcagtgc 
      tccaccaga aaaatcgaa aatgtgtcc gacgttaca 
      gaatgggtg gatggcttg gccaagtgc ccctataac 
      cctgcagcc aacatcacc ggctttatg tcagaacgg 
      ggggagtac tattttggc ggacccacg gatttttcc 
      agctccgat ttcgtcttt tccaaaaat gtgggcacg 
      gtcacgatc aggaccaaa cagtacaat agcttcttg 
      ctgaatgac ccgcagttt gtgggcact tttgagact 
      gagaatttc gtctatttc ttgttcagg gagacggct 
      gtggagtac atgaactgc gggaagact atttattcg 
      cgaatcgcc cgtgtttgc aagaatgat cccgggggg 
      cacaccatg ttgaaggat aactggacg acgttttta 
      aaggcacgg ttaaactgt tcggtcagt ggcgaatac 
      cccttttat ttcgatgaa atccaaagt atggcttac 
      gtgcctgat gaaaacatg gtctatgcc acatttaca 
      acaccgagc aatagtatc gcaggttcc gcaatttgt 
      gcctttaac ttatcgtcg atcgaagcc gcttttgcg 
      ggccctttc aagtacagg gaaaacatg gacagcgct 
      tgggggcag tacaacgtg caccaccgc gaccatttc 
      aactgtaaa tcatcgcca cgaagcagc caccttttg 
      gagacgtcc aaataccag ttaatggac agtgccgtc 
      caggcaacc actctaaac cccttacac gtcgcccaa 
      ttggaacgc ttcactcat attacaatc gacgttctc 
      tcaaccaaa atccacact tccgtccat gtgatctac 
      gtgtccaca accaccggt gtgataaaa aagatctcg 
      gtcttgccc cgaacccag gagacctgc gtggtggaa 
      atctggcaa acggtccaa aaccccgcc acccccgtc 
      aaaagcatc cagttcctc aaagccacg aagtccatc 
      tacgtggcg acggagcac caggtcatg aaaatcccc 
      gccgagcac tgctcccgc cacacctcc aagaccagc 
      tgcctcaac gccatggac ccctactgc ggctggaac 
      gagcgctac gagaactgc tcgacagcc ccgaacgcc 
      gaccccctg gacaagtac tggaagcag gcggtgacc 
      acgtgcccc atcctggac accgtcgtc gacgggggc 
      tggtcctcg tggtcgccg tgggccccc tgcgtgcag 
      cgcagcgcc cccgagtcg gacgccgac acctgcctg 
      tgccaggtg cgcgagtgc aacaacccg gcccccaag 
      aacgggggg ctgccctgc ggggggccc gccatcgcc 
      gtgacgaac tgcacggtg cacgggggc tggtccgtg 
      tggtcggcc tggtccgcc tgctcggcc acctgcggc 
      acggccgtc aaaacgcgg agccggacc tgcaccaac 
      ccggccccg gcgcacggc gggcgcgtc tgcgtgggg 
      caggacagg actgaggcg ttttgcgac gcggagata 
      cccccctgt ccggcgatg ccccaggat gggggctgg 
      ggcgaatgg agcgactgg ggcgactgt tcgcccagt 
      cattgctcc gggtgtaag aggaggaca agggtttgt 
      aataaccca cccccggaa aatgggggg cagttttgt 
      gccgggaat gacgaagag ttcaagttt gagtgtcca 
      gagcaccgg aaaaccgtc agtacgccc tggataagt 
      cttaccaac agctccggg gactacaat aaacggttc 
      aggtgcacg tgcaaggcc ccagtgaag atgtcgtta 
      attaaggtc gcaatgaag gaggaagtt tgtaataat 
      gataactgc tttacgaaa gacagtgaa catcccaag 
      tggagtgcg tgggggccg tggggggag tgttcggtt 
      cagtgcggg ggcggcttg cagaataga acgagatat 
      tgtgaaggg atcggctgc acaggcccc tctgttcaa 
      acaagggag tgcaataaa caccactgc cgggagtgg 
      ggctgctgg tcggaatgg tcaccctgc aacgcctcc 
      tgcggctgg ggcgtcaag accaggcac cgggtctgc 
      ttaggctcg aactgctcc gggcacgac tgggagaag 
      gagccctgc cagaacgcg ccctgcgaa tcagaatcc 
      ctacattac aatgcccct tcaggacag gtcgaagaa 
      acacagtgc ttcaacgtc gaacaatct tttaaataa
    mRNA sequence
    • >TC001449 RA
      tgttttgtt tttgttaat tttaataga cacacaaaa 
      aggattttc tccgcaagc tctctactt tcctgtgaa 
      acagaccca atattcggc aattgattt atcaaactc 
      aatacaaag tcctgacga aatagctat tattttttt 
      tgataaaac ttgaaaacc aacctccga ttcgcaaaa 
      taagtgcga acgtgattc tgatcatgg atcaattac 
      acaacaaat aaatgttcc actaattac atctcctga 
      taccacaat cttgttatc tgctagact gcaagtcta 
      ttgtgaaag catcaaagt ggctactgt tatcactta 
      ctcaattac acaaaacaa aaaacaagt aacagatgt 
      taaagaata tttcgcgat ttatcacac gttcctgca 
      gttcgttca agaccataa aaatgcatg gaaaaagat 
      taaaaccac ataatgtta aagttttat ttttggaga 
      tttgaatgc cagcaattt acacgtgga ttgtagata 
      aaccaagat aaacaggcc tactttcct atcaaagtg 
      gattttctg acaaaagta ccaacaaac aaaaacagc 
      aatagtgca aaatgttgt ataaacgcg gtataatta 
      attgcttaa ttacttagc ataactaac acatgtttt 
      gttgtcttg tgtatgtat ttgcgttaa gttgtgttc 
      ttttttgtg gaaattctt gaaaatctt gcacgtttt 
      tcctcctgg cacggaaat gctaggaga tgcaggttc 
      ggaggcttg gaagactaa tctaatcat agaatacag 
      agtgtctga atcgtaaga tggagctgc gcaaccgga 
      taggcaggc cggagctga gaacagttc tttagaaca 
      gttcataac ccactccat gcaatcgaa taccgttca 
      tgcaatgaa cctaaacgt ttcgcttca cagaaaggc 
      aacacagtg tttataccc aaacaacct agccaaggt 
      cgttccata atttcgttt attcaccga catctgtgc 
      tatattttg tgcagatag ggggttttt cggacgcgt 
      ggttcgact agtgaccgt gatttgtgc ttaaaatgg 
      acgttttta gtgatattt gcagattta taaacagat 
      ttttgatgt gatggaaag ttggacgat gtcttgccg 
      ctctcccct ctttactga cctgtctac ccagctatg 
      ctcatagaa aatcatact tagcatgca gaatggtgc 
      cacatggac ctttaaatt ttttacttt gcctgtcga 
      ttttcctaa cactaactc tatcggcgg agcatgatt 
      ttcgatata tttcgttcc aagatcttc agcaatctt 
      taaacaaat tttcacaag atgaggttg tctcctact 
      cgcagatgc ttttcgacg tggccagga accaagtat 
      tagtcggcg caagagacg tcctctttc gcttatctt 
      tgacgaaac tggagcttt tggagtcgg ccgaatggc 
      ccgcctcca cagacaaac gcatcgaat gcatcaaca 
      aaggccaat cggacgagt tttgccaca actacatca 
      aaattttgc taaccaatg gcaaaaacc tattcgtct 
      gtggcacga acgcattct cgcctcagt gctccacca 
      gaaaaatcg aaaatgtgt ccgacgtta cagaatggg 
      tggatggct tggccaagt gcccctata accctgcag 
      ccaacatca ccggcttta tgtcagaac ggggggagt 
      actattttg gcggaccca cggattttt ccagctccg 
      atttcgtct tttccaaaa atgtgggca cggtcacga 
      tcaggacca aacagtaca atagcttct tgctgaatg 
      acccgcagt ttgtgggca cttttgaga ctgagaatt 
      tcgtctatt tcttgttca gggagacgg ctgtggagt 
      acatgaact gcgggaaga ctatttatt cgcgaatcg 
      cccgtgttt gcaagaatg atcccgggg ggcacacca 
      tgttgaagg ataactgga cgacgtttt taaaggcac 
      ggttaaact gttcggtca gtggcgaat acccctttt 
      atttcgatg aaatccaaa gtatggctt acgtgcctg 
      atgaaaaca tggtctatg ccacattta caacaccga 
      gcaatagta tcgcaggtt ccgcaattt gtgccttta 
      acttatcgt cgatcgaag ccgcttttg cgggccctt 
      tcaagtaca gggaaaaca tggacagcg cttgggggc 
      agtacaacg tgcaccacc gcgaccatt tcaactgta 
      aatcatcgc cacgaagca gccaccttt tggagacgt 
      ccaaatacc agttaatgg acagtgccg tccaggcaa 
      ccactctaa accccttac acgtcgccc aattggaac 
      gcttcactc atattacaa tcgacgttc tctcaacca 
      aaatccaca cttccgtcc atgtgatct acgtgtcca 
      caaccaccg gtgtgataa aaaagatct cggtcttgc 
      cccgaaccc aggagacct gcgtggtgg aaatctggc 
      aaacggtcc aaaaccccg ccacccccg tcaaaagca 
      tccagttcc tcaaagcca cgaagtcca tctacgtgg 
      cgacggagc accaggtca tgaaaatcc ccgccgagc 
      actgctccc gccacacct ccaagacca gctgcctca 
      acgccatgg acccctact gcggctgga acgagcgct 
      acgagaact gctcgacag ccccgaacg ccgaccccc 
      tggacaagt actggaagc aggcggtga ccacgtgcc 
      ccatcctgg acaccgtcg tcgacgggg gctggtcct 
      cgtggtcgc cgtgggccc cctgcgtgc agcgcagcg 
      cccccgagt cggacgccg acacctgcc tgtgccagg 
      tgcgcgagt gcaacaacc cggccccca agaacgggg 
      ggctgccct gcggggggc ccgccatcg ccgtgacga 
      actgcacgg tgcacgggg gctggtccg tgtggtcgg 
      cctggtccg cctgctcgg ccacctgcg gcacggccg 
      tcaaaacgc ggagccgga cctgcacca acccggccc 
      cggcgcacg gcgggcgcg tctgcgtgg ggcaggaca 
      ggactgagg cgttttgcg acgcggaga tacccccct 
      gtccggcga tgccccagg atgggggct ggggcgaat 
      ggagcgact ggggcgact gttcgccca gtcattgct 
      ccgggtgta agaggagga caagggttt gtaataacc 
      cacccccgg aaaatgggg ggcagtttt gtgccggga 
      atgacgaag agttcaagt ttgagtgtc cagagcacc 
      ggaaaaccg tcagtacgc cctggataa gtcttacca 
      acagctccg gggactaca ataaacggt tcaggtgca 
      cgtgcaagg ccccagtga agatgtcgt taattaagg 
      tcgcaatga aggaggaag tttgtaata atgataact 
      gctttacga aagacagtg aacatccca agtggagtg 
      cgtgggggc cgtgggggg agtgttcgg ttcagtgcg 
      ggggcggct tgcagaata gaacgagat attgtgaag 
      ggatcggct gcacaggcc cctctgttc aaacaaggg 
      agtgcaata aacaccact gccgggagt ggggctgct 
      ggtcggaat ggtcaccct gcaacgcct cctgcggct 
      ggggcgtca agaccaggc accgggtct gcttaggct 
      cgaactgct ccgggcacg actgggaga aggagccct 
      gccagaacg cgccctgcg aatcagaat ccctacatt 
      acaatgccc cttcaggac aggtcgaag aaacacagt 
      gcttcaacg tcgaacaat cttttaaat aatgggacg 
      cttaaatct aaatattgt agtgattac gagagtgca 
      acgcttaaa agaaacagt cacacgttg aataacgga 
      cacgtaaag caggactta cttgacgat gataagtat 
      tattacgac tgagttatt ttgataaat gtgatatat 
      gaattttgt aagcagatg tacctgtat atgcacata 
      acagtgaaa caatttgta aaatattaa caaagtctg 
      taaatatcg cctaagtta tgttataac tcacattga 
      gttagtagt gatggttaa taaagtttt tgtaatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY