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Gene information
General information
TC005314 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14535199..14542463
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005314
    iBeetle screen iB_09426

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    tropharium structure irregular - Number of animals showing the phenotype: 3

    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3

    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005314 RA
      MATSFPNMN ITNPIRGLV SKRRIRYKQ DGFNLDLTY 
      ITDSIIAMG YPASNIEGV YRNHIDDVV KLLDSKHNN 
      HYFIYNLCS ERTYDTSKF HNRVQTFPF DDHNPPKIE 
      LIQPFCHSV HDWLSKDPE NVAVVHCKA GKGRTGTMI 
      CCYLLHSGA FATADEALD HYGQARTQD HKGVTIPSQ 
      VRYIRYYET LLRNKLLYV PVSMYIKQI VLEPVPNFA 
      GGQGYVCFS ISQQTLQGQ DDTQQKCTK LYKSGVYEV 
      KRGVSQLLI TMDLCLVLN GDIKIEFYN KSKIGRKER 
      VFQFWFNTF FVMNQEDRT NELIEQNGS GESKQSCVL 
      TLKKDELDI VNKKDKQNK VFSADFRLT LLLEKVPKN 
      QPNWRTYTP EIPRLSQDT PSESSEAEW SEENDSAEE 
      GWESGESTY L
    • >TC005314 RB
      MATSFPNMN ITNPIRGLV SKRRIRYKQ DGFNLDLTY 
      ITDSIIAMG YPASNIEGV YRNHIDDVV KLLDSKHNN 
      HYFIYNLCS ERTYDTSKF HNRVQTFPF DDHNPPKIE 
      LIQPFCHSV HDWLSKDPE NVAVVHCKA GKGRTGTMI 
      CCYLLHSGA FATADEALD HYGQARTQD HKGVTIPSQ 
      VRYIRYYET LLRNKLLYV PVSMYIKQI VLEPVPNFA 
      GGQGYVCFS ISQQTLQGQ DDTQQKCTK LYKSGVYEV 
      KRGVSQLLI TMDLCLVLN GDIKIEFYN KSKIGRKER 
      VFQFWFNTF FVMNQEDRT NELIEQNGS GESKQSCVL 
      TLKKDELDI VNKKDKQNK VFSADFRLT LLLEKVPKN 
      QPNWRTYTP EIPRLSQDT PSESSEAEW SEENDSAEE 
      GWESE
    CDS sequence
    • >TC005314 RA
      atggcaaca tctttcccc aatatgaac atcaccaac 
      cctatacgg gggctggtc agcaagagg aggatccga 
      tacaagcag gacgggttc aatctcgac ctgacatac 
      ataaccgat agcataata gcaatgggt tacccggcg 
      tccaacatc gaaggcgtg taccgcaac cacatcgac 
      gatgtggtg aagctcctc gattcgaaa cacaacaac 
      cactacttc atctacaac ttgtgttcg gaacgcact 
      tacgacacg tccaagttc cacaaccga gtacaaacc 
      tttccgttc gacgaccac aacccgccc aaaatcgag 
      ttgattcag ccgttttgt cacagcgtt cacgattgg 
      ctgagcaaa gatccagag aacgtggcc gtagttcat 
      tgcaaggcg gggaaaggg cgcaccggg accatgatt 
      tgctgttac cttttgcat agtggagct tttgctacc 
      gctgacgaa gccctcgac cattacgga caagcacgg 
      acgcaagat cacaaaggg gtcaccatt cccagccaa 
      gtgcgatat atccgttac tacgagacc ctgcttagg 
      aacaagttg ttgtatgtg cctgtgtcg atgtacatc 
      aagcagatt gttttggag ccggtgccg aattttgca 
      ggaggccaa ggctatgtg tgtttttcg atatcgcaa 
      cagacgcta caaggacaa gacgataca caacaaaag 
      tgcaccaaa ttgtataaa agcggagtc tacgaagtg 
      aagagaggt gtatcgcag ttgcttatc acgatggat 
      ttgtgtttg gtgctaaat ggagatatt aaaatcgag 
      ttttataac aagtcgaag attggaagg aaagagaga 
      gtgtttcag ttttggttt aatacattt tttgtgatg 
      aaccaagag gacagaacg aacgaattg attgaacaa 
      aacggaagt ggtgaatcg aaacagagt tgcgtgttg 
      acgttaaaa aaggacgaa cttgatatc gttaataaa 
      aaagacaaa cagaataaa gtgtttagt gctgacttt 
      cggttgact ttgcttctt gaaaaagtt ccaaagaac 
      caaccgaat tggaggaca tatacccct gaaatcccc 
      cgattatct caagacaca cctagcgaa agttcagaa 
      gccgaatgg tcagaagaa aacgattca gcagaagaa 
      ggctgggaa agcggggag tccacgtac ctgtga
    • >TC005314 RB
      atggcaaca tctttcccc aatatgaac atcaccaac 
      cctatacgg gggctggtc agcaagagg aggatccga 
      tacaagcag gacgggttc aatctcgac ctgacatac 
      ataaccgat agcataata gcaatgggt tacccggcg 
      tccaacatc gaaggcgtg taccgcaac cacatcgac 
      gatgtggtg aagctcctc gattcgaaa cacaacaac 
      cactacttc atctacaac ttgtgttcg gaacgcact 
      tacgacacg tccaagttc cacaaccga gtacaaacc 
      tttccgttc gacgaccac aacccgccc aaaatcgag 
      ttgattcag ccgttttgt cacagcgtt cacgattgg 
      ctgagcaaa gatccagag aacgtggcc gtagttcat 
      tgcaaggcg gggaaaggg cgcaccggg accatgatt 
      tgctgttac cttttgcat agtggagct tttgctacc 
      gctgacgaa gccctcgac cattacgga caagcacgg 
      acgcaagat cacaaaggg gtcaccatt cccagccaa 
      gtgcgatat atccgttac tacgagacc ctgcttagg 
      aacaagttg ttgtatgtg cctgtgtcg atgtacatc 
      aagcagatt gttttggag ccggtgccg aattttgca 
      ggaggccaa ggctatgtg tgtttttcg atatcgcaa 
      cagacgcta caaggacaa gacgataca caacaaaag 
      tgcaccaaa ttgtataaa agcggagtc tacgaagtg 
      aagagaggt gtatcgcag ttgcttatc acgatggat 
      ttgtgtttg gtgctaaat ggagatatt aaaatcgag 
      ttttataac aagtcgaag attggaagg aaagagaga 
      gtgtttcag ttttggttt aatacattt tttgtgatg 
      aaccaagag gacagaacg aacgaattg attgaacaa 
      aacggaagt ggtgaatcg aaacagagt tgcgtgttg 
      acgttaaaa aaggacgaa cttgatatc gttaataaa 
      aaagacaaa cagaataaa gtgtttagt gctgacttt 
      cggttgact ttgcttctt gaaaaagtt ccaaagaac 
      caaccgaat tggaggaca tatacccct gaaatcccc 
      cgattatct caagacaca cctagcgaa agttcagaa 
      gccgaatgg tcagaagaa aacgattca gcagaagaa 
      ggctgggaa agcgaataa
    mRNA sequence
    • >TC005314 RA
      gattgtatg taaagtctt gtctgtaga atgttcagt 
      catcgtttc gcaatcctc tgaaatgtc actagcggt 
      gaatcgtaa ttttaatga gagaattcg tccggaaat 
      cgcggtttt ctttcattc attcgcgtg aaattatga 
      aattcccgg tcttttgcg cgccctcta tcggaattc 
      ttgacacca gtcgacgcg atgtagttg tctacattg 
      actgaatct gtgtcgaca gaccgtgta gtgccgtaa 
      aaaagacaa agacgtttg agtgtggca gcgatttag 
      tccgaaaac agccccaaa ccggctttg aatcccttg 
      atcgaggtg ctcagagca gccccggcc tccgaaatg 
      gggacgagt gcgtgatgt ctacaatca ggacggctg 
      tggaacgca tgtggtgtg attgttcac cggaggcac 
      tgtccgttt aaagggcct ctggagggg ctgcgcccc 
      gggggctga cacagcgga cacgagggt tcaatggta 
      ggcacgcac gccagcgca cgagctatc aacatttcg 
      tggactttt gccgagact tttttatgg ggctgtgct 
      ccagctgcg gcaaatcaa aggaccgat ttaaagata 
      aatcgtcga aaatatgtg gccaactac attcagtcg 
      ttacacccc ttcatgtgt cacatgagc ccacaagtt 
      cgaacaatt tgaacgggc atttgcctg ttcaaaatc 
      acgtcaacg caagaccgt actcttcag gtaatatgg 
      caacatctt tccccaata tgaacatca ccaacccta 
      tacgggggc tggtcagca agaggagga tccgataca 
      agcaggacg ggttcaatc tcgacctga catacataa 
      ccgatagca taatagcaa tgggttacc cggcgtcca 
      acatcgaag gcgtgtacc gcaaccaca tcgacgatg 
      tggtgaagc tcctcgatt cgaaacaca acaaccact 
      acttcatct acaacttgt gttcggaac gcacttacg 
      acacgtcca agttccaca accgagtac aaacctttc 
      cgttcgacg accacaacc cgcccaaaa tcgagttga 
      ttcagccgt tttgtcaca gcgttcacg attggctga 
      gcaaagatc cagagaacg tggccgtag ttcattgca 
      aggcgggga aagggcgca ccgggacca tgatttgct 
      gttaccttt tgcatagtg gagcttttg ctaccgctg 
      acgaagccc tcgaccatt acggacaag cacggacgc 
      aagatcaca aaggggtca ccattccca gccaagtgc 
      gatatatcc gttactacg agaccctgc ttaggaaca 
      agttgttgt atgtgcctg tgtcgatgt acatcaagc 
      agattgttt tggagccgg tgccgaatt ttgcaggag 
      gccaaggct atgtgtgtt tttcgatat cgcaacaga 
      cgctacaag gacaagacg atacacaac aaaagtgca 
      ccaaattgt ataaaagcg gagtctacg aagtgaaga 
      gaggtgtat cgcagttgc ttatcacga tggatttgt 
      gtttggtgc taaatggag atattaaaa tcgagtttt 
      ataacaagt cgaagattg gaaggaaag agagagtgt 
      ttcagtttt ggtttaata cattttttg tgatgaacc 
      aagaggaca gaacgaacg aattgattg aacaaaacg 
      gaagtggtg aatcgaaac agagttgcg tgttgacgt 
      taaaaaagg acgaacttg atatcgtta ataaaaaag 
      acaaacaga ataaagtgt ttagtgctg actttcggt 
      tgactttgc ttcttgaaa aagttccaa agaaccaac 
      cgaattgga ggacatata cccctgaaa tcccccgat 
      tatctcaag acacaccta gcgaaagtt cagaagccg 
      aatggtcag aagaaaacg attcagcag aagaaggct 
      gggaaagcg gggagtcca cgtacctgt gaatgggag 
      gctcaaagg gcatgcatc gtacaaaac tgtaaatat 
      tgcatgtag agaagggat tgtgtcatt atttttttt 
      ttgatcatt cattcatcc gcttgcttt ttttaaacc 
      aagtttaaa atcagtcaa aacactttt taattatga 
      gagtacaaa tgctttttt gatctcatt tcattttat 
      cttgccaat gttagtact ttggttacc ggttattat 
      aagattttt gccattttc cccctaacc ctctcccta 
      gtttttttt gtagggaaa tttagaaat gaactaatt 
      gtgaaccga attgccagt acaatatga agactgaat 
      gaagaataa tttaacgat ccaaaactg tatacgatt 
      tcttgttca ggcttcagt tctaatttt ccctaattt 
      tataattgt aataactta acgaaaatt tctctcttc 
      cctagaaaa ggctgttaa attatttta ttaagacca 
      ataatttgt atgctcgaa ttatttagt gcatagttt 
      ttttctcaa tgtattttt ttacattag gaagtacct 
      tcttataag ttaaactta gaacatgtt ttaattttt 
      ctctttgtg tttcaacaa tagaaacaa gttgtactt 
      acgatgcca aattttgtt aatgtttta actgatttt 
      aattgaaaa caatctcga acccagtgt atggtcttc 
      agaaaaata ttttttaac atggtacca accagtttt 
      ataatataa aaattgtat tataaggtt aaagtaaca 
      aaaattatg tgactgcgt cattattat tgaacgaaa 
      ataaagtaa accactatt t
    • >TC005314 RB
      gattgtatg taaagtctt gtctgtaga atgttcagt 
      catcgtttc gcaatcctc tgaaatgtc actagcggt 
      gaatcgtaa ttttaatga gagaattcg tccggaaat 
      cgcggtttt ctttcattc attcgcgtg aaattatga 
      aattcccgg tcttttgcg cgccctcta tcggaattc 
      ttgacacca gtcgacgcg atgtagttg tctacattg 
      actgaatct gtgtcgaca gaccgtgta gtgccgtaa 
      aaaagacaa agacgtttg agtgtggca gcgatttag 
      tccgaaaac agccccaaa ccggctttg aatcccttg 
      atcgaggtg ctcagagca gccccggcc tccgaaatg 
      gggacgagt gcgtgatgt ctacaatca ggacggctg 
      tggaacgca tgtggtgtg attgttcac cggaggcac 
      tgtccgttt aaagggcct ctggagggg ctgcgcccc 
      gggggctga cacagcgga cacgagggt tcaatggta 
      ggcacgcac gccagcgca cgagctatc aacatttcg 
      tggactttt gccgagact tttttatgg ggctgtgct 
      ccagctgcg gcaaatcaa aggaccgat ttaaagata 
      aatcgtcga aaatatgtg gccaactac attcagtcg 
      ttacacccc ttcatgtgt cacatgagc ccacaagtt 
      cgaacaatt tgaacgggc atttgcctg ttcaaaatc 
      acgtcaacg caagaccgt actcttcag gtaatatgg 
      caacatctt tccccaata tgaacatca ccaacccta 
      tacgggggc tggtcagca agaggagga tccgataca 
      agcaggacg ggttcaatc tcgacctga catacataa 
      ccgatagca taatagcaa tgggttacc cggcgtcca 
      acatcgaag gcgtgtacc gcaaccaca tcgacgatg 
      tggtgaagc tcctcgatt cgaaacaca acaaccact 
      acttcatct acaacttgt gttcggaac gcacttacg 
      acacgtcca agttccaca accgagtac aaacctttc 
      cgttcgacg accacaacc cgcccaaaa tcgagttga 
      ttcagccgt tttgtcaca gcgttcacg attggctga 
      gcaaagatc cagagaacg tggccgtag ttcattgca 
      aggcgggga aagggcgca ccgggacca tgatttgct 
      gttaccttt tgcatagtg gagcttttg ctaccgctg 
      acgaagccc tcgaccatt acggacaag cacggacgc 
      aagatcaca aaggggtca ccattccca gccaagtgc 
      gatatatcc gttactacg agaccctgc ttaggaaca 
      agttgttgt atgtgcctg tgtcgatgt acatcaagc 
      agattgttt tggagccgg tgccgaatt ttgcaggag 
      gccaaggct atgtgtgtt tttcgatat cgcaacaga 
      cgctacaag gacaagacg atacacaac aaaagtgca 
      ccaaattgt ataaaagcg gagtctacg aagtgaaga 
      gaggtgtat cgcagttgc ttatcacga tggatttgt 
      gtttggtgc taaatggag atattaaaa tcgagtttt 
      ataacaagt cgaagattg gaaggaaag agagagtgt 
      ttcagtttt ggtttaata cattttttg tgatgaacc 
      aagaggaca gaacgaacg aattgattg aacaaaacg 
      gaagtggtg aatcgaaac agagttgcg tgttgacgt 
      taaaaaagg acgaacttg atatcgtta ataaaaaag 
      acaaacaga ataaagtgt ttagtgctg actttcggt 
      tgactttgc ttcttgaaa aagttccaa agaaccaac 
      cgaattgga ggacatata cccctgaaa tcccccgat 
      tatctcaag acacaccta gcgaaagtt cagaagccg 
      aatggtcag aagaaaacg attcagcag aagaaggct 
      gggaaagcg aataaaatt cagaaacaa ttagaggaa 
      aatccagtg atgttattt gtaataatt attaaaatt 
      tcaaagtgt cattgttgc tgtttgaac agaaaaaat 
      attattgtc tctatctgt taaaaagag aaaaaagta 
      tttatttca ttaggatgt agttgtata gttgacgag 
      tataaagtg tgttgtgta agctacaaa aatttggct 
      ttgtaaaag aatatattt tccttgatc tgttttggt 
      tttttcgaa aagactttt tcgtttttg ttgttttta 
      ttttaaatc caagtacaa cgcttgcgc aatccattt 
      cattcattt cgtgttggt attaactct ctctctctc 
      tctctctct atgatcgaa tcatctgca tgtgtcttt 
      actaatcta tgtttgggg ttaatctca tgcagtcct 
      ggatgaagg cgagaccct aatggatga tcctcgagg 
      tgttggcta cagaatatt atcagaatg cgagttagc 
      ttcgcaact aacaacatc aagcagcgg cgaccaaca 
      accggaatt gttgactgc gtaactcca tgtctaatc 
      gtgcattta ccagtattt ctagattcg tttttgttt 
      atctaccct gggcgtccc tcaaggctc gcatccgag 
      actgtttct attcgcaag taataataa cacaaaatc 
      gagtacaaa aagcagccg gattacagt ttattaaat 
      tggtgtgtc agtcagata gattattag ggaacagcg 
      acgtctacc tcaaaattt tttcttcgt aggacaatc 
      acatttttt gcttagtga tgattctgg ttttttgaa 
      tttaataaa ctgtattgt gtaagctca agttgtaag 
      aatgtattt tccgttaat gagacaatg attaaaaag 
      atataataa ttctctcgt tggtctgat ttttattat 
      tattttttt tttcgtata aaaaccctc cgaacaaat 
      acggatttc aggtaatta tgtgtaaat tgtgcttgt 
      gcttatttg tgttgtgtg tacaatgtt gggaaatca 
      gtcttactt aaatttatt tctagggga gtccacgta 
      cctgtgaat gggaggctc aaagggcat gcatcgtac 
      aaaactgta aatattgca tgtagagaa gggattgtg 
      tcattattt tttttttga tcattcatt catccgctt 
      gcttttttt aaaccaagt ttaaaatca gtcaaaaca 
      ctttttaat tatgagagt acaaatgct tttttgatc 
      tcatttcat tttatcttg ccaatgtta gtactttgg 
      ttaccggtt attataaga tttttgcca ttttccccc 
      taaccctct ccctagttt tttttgtag ggaaattta 
      gaaatgaac taattgtga accgaattg ccagtacaa 
      tatgaagac tgaatgaag aataattta acgatccaa 
      aactgtata cgatttctt gttcaggct tcagttcta 
      attttccct aattttata attgtaata acttaacga 
      aaatttctc tcttcccta gaaaaggct gttaaatta 
      ttttattaa gaccaataa tttgtatgc tcgaattat 
      ttag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY