Status
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Gene information
General information
TC031365
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011186,TC011187
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3128735..3132262
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031365
    iBeetle screen iB_09422

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031365 RA
      MGERKGTNK YYPPDYDPK AGGLNKFLG THALRERAR 
      KIHLGIIIV RFEMPYNIW CEGCNNHIG MGVRYNAEK 
      TKLGMYYTT PVYQFKMKC HLCDNHIVI KTDPGNLEY 
      VIVSGARRQ ENRWDPTQN EQVVPETKE TQKRMFDDS 
      MFKLEHGSE DKSSYENSK PRLIQLFNR NESTWSDNY 
      SANQRLRQI FRKENCELK KIDGQNTAL LKKSSLDIP 
      LLAETEEDK KMASLLTLK LKPHTDIVQ NTELLRKRI 
      IASSSLPTS NFSLKKELK AVKILKETP KKDLGIVKR 
      SSSTAPASA TSDKKPKLV NLVGYGSGR NRRLPRTLR 
      NSIPPMMSG FGGDMSHSR YAMLGGGVP TLPIVEPAA 
      APKHFPFPN TPVDDDLFF EFLMFMFSA VCAGLQFLH 
      LYRTVWWLP HSYNRQAMN FYLIDLNLV AFIVILLSR 
      RLIYIVGCR VLERSVAAK FLYLSYTFY RIILFSILF 
      TLLSWCAYY VIQNHPIVN ILYLCYPVL VYLILFGCK 
      VTAFFELVT WNMSSIPPL HACRSNAAE VREEVENLK 
      TNFNNRMKQ ILFSSVVNA YYTGFIPCC FAQNHLHYD 
      VYWATQHVI FIFLSSFVA YATHILSLR YCDILHRSA 
      LHLGIWDKL ETRNMLLVN NNWNEETLW PSGALVKHG 
      RDLYRAQGE CNASEPGNT TYSRFYLMF KNPSYSLSI 
      LLSFHVAMV LLQLVLLVR SIVWYNIIS ITFMLFFNY 
      YVLYKVGRD YLVSSKMYE EERAMHNKL NLH
    CDS sequence
    • >TC031365 RA
      atgggtgag cgtaaaggc acgaacaag tactacccc 
      ccggattac gaccctaag gccgggggg ctcaacaaa 
      tttctgggg actcatgcc ctccgcgaa agggcccgc 
      aaaatccac ctagggatt ataatcgtg cgatttgag 
      atgccctac aatatctgg tgcgagggc tgcaacaac 
      cacataggc atgggggtg cggtataac gccgagaaa 
      accaaactt gggatgtac tacaccact cctgtatac 
      caatttaaa atgaagtgc cacctgtgt gacaatcac 
      attgttata aaaacggac cctgggaac cttgaatat 
      gtgatagtg tcaggtgcc aggagacag gaaaacagg 
      tgggacccc acacagaat gagcaagtg gtgccggaa 
      acgaaggaa acgcagaaa cggatgttt gacgattct 
      atgtttaaa ttagaacat ggaagtgag gataaaagt 
      agttatgag aactcaaaa ccgcggcta attcaatta 
      ttcaatcgg aatgaatca acttggtct gataattat 
      tcggccaac cagagatta agacagata tttaggaag 
      gaaaattgc gaattgaag aaaatcgac ggccagaac 
      acagctcta ttaaaaaaa tcaagcctg gacattcca 
      ctcttagcc gagactgaa gaagacaag aaaatggca 
      tctttatta acactaaaa ctgaagccc catacagat 
      attgttcaa aatactgag ctactccgc aaacggata 
      atagcgagt tcctcttta ccgacatca aatttttcg 
      ttaaaaaag gaactcaaa gccgtcaag atcttgaaa 
      gaaacgccc aaaaaagac ctaggaatt gtaaaacgg 
      tcaagttcg accgcccca gccagtgct acaagtgat 
      aagaaaccc aaactagtc aatttagtt ggttacggt 
      tcagggaga aatcgccgc ttgcctcgc acgcttcgc 
      aactccatc ccgccgatg atgtccggc ttcgggggc 
      gacatgtcg cacagcagg tatgccatg ttggggggc 
      ggagtaccg acgcttcca attgtggag ccagcagcg 
      gcccccaaa cacttcccg tttccgaat acaccggtc 
      gatgatgat ttgtttttc gagtttttg atgttcatg 
      ttcagtgcg gtgtgtgct gggttgcag tttttgcac 
      ttgtatcgg accgtgtgg tggttgccc cattcgtac 
      aatcgccag gctatgaat ttttaccta attgatctc 
      aatttagtc gcatttatt gtaatttta ttgtcgcga 
      cgtttgatt tatattgtg gggtgtaga gttcttgag 
      aggtcggtg gcggccaaa ttcttgtac ctgtcatac 
      accttttat cgaattatt ttgttttct atacttttt 
      actctgctc tcatggtgt gcctattac gtcatccaa 
      aatcaccca attgtgaat attttatat ttatgttac 
      ccagtcctt gtatatttg attttattt ggctgtaaa 
      gttacggca tttttcgag ttggtaact tggaacatg 
      tcctcaatt ccgccactg cacgcttgc cgttcaaat 
      gcggccgaa gttcgtgaa gaagtggaa aatctcaaa 
      actaatttt aacaatcgc atgaagcaa attctcttc 
      agttcagtc gtcaatgcc tactatact ggtttcatc 
      ccttgttgc tttgcccag aaccacctc cattacgac 
      gtttactgg gccactcaa cacgtaatt tttattttt 
      ttgagtagt ttcgtggct tacgccacc catattttg 
      tccctgcgt tactgcgat attttgcac agatcggcc 
      ctacatttg ggcatctgg gacaagcta gagaccagg 
      aatatgtta ctggtgaat aataactgg aatgaggag 
      actctgtgg ccttcaggg gcccttgtt aagcacggc 
      agggacttg tatagggct caaggagag tgcaatgct 
      agcgaacca ggaaataca acctattcg aggttctat 
      ctcatgttc aaaaatccg agttattcg ttgagtatc 
      ttgttgtcg tttcatgtg gccatggtt ttgctgcag 
      ttggtgtta ttggttcgt agcatcgtg tggtacaat 
      attatttcg ataacgttt atgctgttt tttaattat 
      tacgttctg tataaagtg ggccgtgat tatcttgtc 
      agttcgaaa atgtacgag gaagaacga gcgatgcac 
      aataaactt aatttacat taa
    mRNA sequence
    • >TC031365 RA
      gtcaacaag tcaagttgc agatatttg ttttgtggc 
      caattttgg ggcacaatg ggtgagcgt aaaggcacg 
      aacaagtac taccccccg gattacgac cctaaggcc 
      ggggggctc aacaaattt ctggggact catgccctc 
      cgcgaaagg gcccgcaaa atccaccta gggattata 
      atcgtgcga tttgagatg ccctacaat atctggtgc 
      gagggctgc aacaaccac ataggcatg ggggtgcgg 
      tataacgcc gagaaaacc aaacttggg atgtactac 
      accactcct gtataccaa tttaaaatg aagtgccac 
      ctgtgtgac aatcacatt gttataaaa acggaccct 
      gggaacctt gaatatgtg atagtgtca ggtgccagg 
      agacaggaa aacaggtgg gaccccaca cagaatgag 
      caagtggtg ccggaaacg aaggaaacg cagaaacgg 
      atgtttgac gattctatg tttaaatta gaacatgga 
      agtgaggat aaaagtagt tatgagaac tcaaaaccg 
      cggctaatt caattattc aatcggaat gaatcaact 
      tggtctgat aattattcg gccaaccag agattaaga 
      cagatattt aggaaggaa aattgcgaa ttgaagaaa 
      atcgacggc cagaacaca gctctatta aaaaaatca 
      agcctggac attccactc ttagccgag actgaagaa 
      gacaagaaa atggcatct ttattaaca ctaaaactg 
      aagccccat acagatatt gttcaaaat actgagcta 
      ctccgcaaa cggataata gcgagttcc tctttaccg 
      acatcaaat ttttcgtta aaaaaggaa ctcaaagcc 
      gtcaagatc ttgaaagaa acgcccaaa aaagaccta 
      ggaattgta aaacggtca agttcgacc gccccagcc 
      agtgctaca agtgataag aaacccaaa ctagtcaat 
      ttagttggt tacggttca gggagaaat cgccgcttg 
      cctcgcacg cttcgcaac tccatcccg ccgatgatg 
      tccggcttc gggggcgac atgtcgcac agcaggtat 
      gccatgttg gggggcgga gtaccgacg cttccaatt 
      gtggagcca gcagcggcc cccaaacac ttcccgttt 
      ccgaataca ccggtcgat gatgatttg tttttcgag 
      tttttgatg ttcatgttc agtgcggtg tgtgctggg 
      ttgcagttt ttgcacttg tatcggacc gtgtggtgg 
      ttgccccat tcgtacaat cgccaggct atgaatttt 
      tacctaatt gatctcaat ttagtcgca tttattgta 
      attttattg tcgcgacgt ttgatttat attgtgggg 
      tgtagagtt cttgagagg tcggtggcg gccaaattc 
      ttgtacctg tcatacacc ttttatcga attattttg 
      ttttctata ctttttact ctgctctca tggtgtgcc 
      tattacgtc atccaaaat cacccaatt gtgaatatt 
      ttatattta tgttaccca gtccttgta tatttgatt 
      ttatttggc tgtaaagtt acggcattt ttcgagttg 
      gtaacttgg aacatgtcc tcaattccg ccactgcac 
      gcttgccgt tcaaatgcg gccgaagtt cgtgaagaa 
      gtggaaaat ctcaaaact aattttaac aatcgcatg 
      aagcaaatt ctcttcagt tcagtcgtc aatgcctac 
      tatactggt ttcatccct tgttgcttt gcccagaac 
      cacctccat tacgacgtt tactgggcc actcaacac 
      gtaattttt atttttttg agtagtttc gtggcttac 
      gccacccat attttgtcc ctgcgttac tgcgatatt 
      ttgcacaga tcggcccta catttgggc atctgggac 
      aagctagag accaggaat atgttactg gtgaataat 
      aactggaat gaggagact ctgtggcct tcaggggcc 
      cttgttaag cacggcagg gacttgtat agggctcaa 
      ggagagtgc aatgctagc gaaccagga aatacaacc 
      tattcgagg ttctatctc atgttcaaa aatccgagt 
      tattcgttg agtatcttg ttgtcgttt catgtggcc 
      atggttttg ctgcagttg gtgttattg gttcgtagc 
      atcgtgtgg tacaatatt atttcgata acgtttatg 
      ctgtttttt aattattac gttctgtat aaagtgggc 
      cgtgattat cttgtcagt tcgaaaatg tacgaggaa 
      gaacgagcg atgcacaat aaacttaat ttacattaa 
      attacttgt tttatttcg tggatttta tcccctccg 
      aataacgga cac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY