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Gene information
General information
TC006774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14020340..14023161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006774
    iBeetle screen iB_09416

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006774 RA
      MKIIARSVT LALGLSVCG ISGYLLYLL LKKDDDDDY 
      TDTLSRSSK FKILEVPVH KDMVKVLIG RGGKNIKLI 
      QEQSNTRIN FKEREGQQE AICVIRGTI EACNIAENL 
      VQEFVNNQP VLECEDVYV PQGCIARII GRDGDRIRE 
      ICCKSGAKV TVDDNRGAV NRRVSIKGT REQIVVAKS 
      LIEEIVEQS HKTQVQIEA SLAKREPRL PPKASESPK 
      PVESPKVER ISPVPGQPD AQFEVYVSA MVDPSRFWL 
      QIVGPKATE LDVLVEEMT EYYRKQENR ESHILNKVT 
      KGDLVAAVF QYDSKWYRA EVLSLSDDN PPQAELYYV 
      DYGDTDLVP VEELYELRT DFLRLHFQA IECFLARVD 
      PVGESWSVE AVDKFEEWT HVAQWKKLS AKINGYCVR 
      EKTRAKREG SPVPGVDLF DVNGEKDVD IAQELVNQG 
      FAVFKRDVD LRPSSRATS VSNISVASS S
    CDS sequence
    • >TC006774 RA
      atgaagata atcgcaaga agtgtaact ttggctttg 
      ggcttatca gtgtgcggc atttcgggg tatttgctg 
      tacctcttg ctcaaaaaa gacgacgat gacgattac 
      actgacacc ctcagccgt agctcaaaa ttcaaaatc 
      ctggaagtc ccagttcac aaagacatg gttaaagtc 
      ctcattgga cgcgggggc aaaaacatc aaactgatc 
      caagagcag tcaaacact cgcatcaac ttcaaagaa 
      cgcgaaggc caacaggag gcaatttgc gtgattcgg 
      ggcacaatt gaggcctgc aatattgcg gaaaatttg 
      gtgcaagaa ttcgtaaac aaccaaccg gtgcttgaa 
      tgtgaggac gtttacgtg cctcaaggc tgcattgcc 
      aggatcata gggcgcgac ggcgaccga attcgcgag 
      atttgctgc aaatcgggg gcgaaagtg acagttgat 
      gacaacagg ggggctgtc aatagacgg gtctcaatc 
      aaagggacg agggaacaa atcgtcgtt gccaaatca 
      ttgattgag gagatcgtg gagcagtcg cacaaaacc 
      caagtccag attgaggca agtctggcc aagcgggag 
      ccccggctc cccccgaag gccagcgag agccccaag 
      ccggtggag agccccaaa gtcgaaagg atctctcca 
      gttccaggt caaccggac gctcaattc gaggtgtat 
      gtctcggca atggtggac ccgtcccgc ttctggctc 
      caaatagtg ggccccaaa gcgacggaa ctcgacgtt 
      ttggtcgaa gaaatgacc gaatactac cgcaagcag 
      gaaaatcgc gaatcccac attttgaat aaagtgact 
      aaaggcgat cttgtcgct gctgtgttc caatacgac 
      agcaaatgg taccgggct gaagtgctc agtctgagt 
      gacgataac ccgccccaa gctgaactc tactatgtt 
      gattacggg gacacggat ttggtcccc gttgaggaa 
      ttgtacgag ttgaggacc gatttcctc cgactgcat 
      tttcaagca attgagtgt tttctagca cgagttgat 
      cccgtggga gagagttgg tcggtggaa gccgtggat 
      aaattcgaa gagtggaca catgtggcg cagtggaag 
      aaactgtcg gcgaaaatt aacggatat tgtgttagg 
      gagaagacg agggcgaag cgcgagggc tctccggtc 
      cccggtgtt gatttgttc gatgtgaac ggtgaaaag 
      gacgtcgat atcgcacaa gaattggtt aatcaaggg 
      ttcgctgtt tttaaaaga gacgtggat ttgaggccg 
      agcagtcgc gccacgagt gttagtaat attagtgta 
      gcttcgagt tcgtag
    mRNA sequence
    • >TC006774 RA
      tcttgagtg tcacttttc taaaatcaa atgtgtcca 
      gaaagaggt taggttttg taaaaatag aaatttatt 
      ttaaaccat caaaataaa aaataattc ggtaacgta 
      acgtgtgat ttcgcaccg gcatgaaga taatcgcaa 
      gaagtgtaa ctttggctt tgggcttat cagtgtgcg 
      gcatttcgg ggtatttgc tgtacctct tgctcaaaa 
      aagacgacg atgacgatt acactgaca ccctcagcc 
      gtagctcaa aattcaaaa tcctggaag tcccagttc 
      acaaagaca tggttaaag tcctcattg gacgcgggg 
      gcaaaaaca tcaaactga tccaagagc agtcaaaca 
      ctcgcatca acttcaaag aacgcgaag gccaacagg 
      aggcaattt gcgtgattc ggggcacaa ttgaggcct 
      gcaatattg cggaaaatt tggtgcaag aattcgtaa 
      acaaccaac cggtgcttg aatgtgagg acgtttacg 
      tgcctcaag gctgcattg ccaggatca tagggcgcg 
      acggcgacc gaattcgcg agatttgct gcaaatcgg 
      gggcgaaag tgacagttg atgacaaca ggggggctg 
      tcaatagac gggtctcaa tcaaaggga cgagggaac 
      aaatcgtcg ttgccaaat cattgattg aggagatcg 
      tggagcagt cgcacaaaa cccaagtcc agattgagg 
      caagtctgg ccaagcggg agccccggc tccccccga 
      aggccagcg agagcccca agccggtgg agagcccca 
      aagtcgaaa ggatctctc cagttccag gtcaaccgg 
      acgctcaat tcgaggtgt atgtctcgg caatggtgg 
      acccgtccc gcttctggc tccaaatag tgggcccca 
      aagcgacgg aactcgacg ttttggtcg aagaaatga 
      ccgaatact accgcaagc aggaaaatc gcgaatccc 
      acattttga ataaagtga ctaaaggcg atcttgtcg 
      ctgctgtgt tccaatacg acagcaaat ggtaccggg 
      ctgaagtgc tcagtctga gtgacgata acccgcccc 
      aagctgaac tctactatg ttgattacg gggacacgg 
      atttggtcc ccgttgagg aattgtacg agttgagga 
      ccgatttcc tccgactgc attttcaag caattgagt 
      gttttctag cacgagttg atcccgtgg gagagagtt 
      ggtcggtgg aagccgtgg ataaattcg aagagtgga 
      cacatgtgg cgcagtgga agaaactgt cggcgaaaa 
      ttaacggat attgtgtta gggagaaga cgagggcga 
      agcgcgagg gctctccgg tccccggtg ttgatttgt 
      tcgatgtga acggtgaaa aggacgtcg atatcgcac 
      aagaattgg ttaatcaag ggttcgctg tttttaaaa 
      gagacgtgg atttgaggc cgagcagtc gcgccacga 
      gtgttagta atattagtg tagcttcga gttcgtagt 
      tttttaccc aaatttttc gaaataaat ttttatcgc 
      cctgttcct caagttact taattcatt tttacattg 
      ctcttattt ttatttatt tttacgttt taggtgcaa 
      ttaattatt tttttaata ttgagtaac cctttttta 
      aattttgaa agtttgtgc cttaatgat tatcttcaa 
      tattgtgat cttgcccga gattccatt tatatttcg 
      ttgaataaa ccctcttaa gttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY