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Gene information
General information
TC005370 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13996218..13998278
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005370
    iBeetle screen iB_09415

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005370 RA
      MSDQPHHPS ENSTTQAKL IDGKKISQD FQKELKVKV 
      DEWVNSGHR RPVLIAVLV GDDPASQKY VHNKMLAAR 
      NVGITSETI RMPKTSTEA EVLAKVHQL NEDPQVDGI 
      LVQLPVPEG IDERKVCNA VDPRKDVDG FHILNVGKL 
      TLNMDTFVP ATALGVIEL IKRSNIKTF GKNVLICGR 
      SKNVGLPMA MLLHSDARN ELSGGEATV VLCHRNTPP 
      EELEFFAKR ADIIISATG VVGLIKPSM IKPGACVID 
      VGITRITTP EGKSKIVGD VDFEGVSKI AGHITPVPG 
      GVGPMTVAM LMHNTFKAA MDLAKSS
    CDS sequence
    • >TC005370 RA
      atgtcggac caaccccat catccatcg gaaaattcc 
      accactcaa gccaaactg atcgacggg aaaaaaatc 
      tctcaagac ttccagaag gagctgaag gtgaaggta 
      gacgaatgg gtcaactcc ggtcaccgg cgccctgtc 
      ctgatcgcc gtcctcgtc ggagacgat ccagcgagt 
      cagaaatac gtccataac aaaatgctg gcggcccgt 
      aacgtgggc atcaccagc gagaccatc cgcatgccc 
      aaaacctcc accgaggcg gaagtcctc gccaaggtg 
      caccagctc aacgaggac ccccaggtg gacggcatc 
      ctggtgcaa ctgccggtg ccggaaggc atcgacgag 
      cgcaaggtc tgcaacgcc gtggacccc cgcaaggac 
      gtggacggc ttccacatc ctcaacgtg gggaagctc 
      accctcaac atggacacc ttcgtccca gccacggcc 
      ttgggagtc atcgagctg atcaagcgc tcgaatatc 
      aagaccttc ggaaaaaat gtgcttatt tgcgggcgc 
      tcgaagaac gtggggctg cctatggcg atgctgttg 
      cattcggac gcgcggaat gaactctcg ggaggggag 
      gccaccgtg gtgctctgc cacaggaat acgcctcca 
      gaagaattg gagtttttc gcaaaaagg gccgatatt 
      attataagt gctacgggg gttgtgggg ctcattaaa 
      ccgagtatg attaagcca ggggcttgt gttattgat 
      gtgggcatt acgaggatt acgaccccg gaaggcaag 
      agtaaaatt gttggagat gttgatttt gaaggggtg 
      agcaaaatt gcggggcat attacaccg gtcccgggg 
      ggcgttggc cccatgact gtcgccatg ctcatgcac 
      aacaccttt aaagctgcc atggatctt gcgaaaagt 
      tcataa
    mRNA sequence
    • >TC005370 RA
      ttggagcct agagcttaa tacccttgc aaggttact 
      ttatctatg ggcaatacg tctggcaac gtggccact 
      aatcacagg tagagggtg aagacaatt caaaagaaa 
      gttttgccg gcttggcaa gtcccgtta tcactttat 
      caagtcttt agtcccaca attgtagtt aaaaatgtc 
      ggaccaacc ccatcatcc atcggaaaa ttccaccac 
      tcaagccaa actgatcga cgggaaaaa aatctctca 
      agacttcca gaaggagct gaaggtgaa ggtagacga 
      atgggtcaa ctccggtca ccggcgccc tgtcctgat 
      cgccgtcct cgtcggaga cgatccagc gagtcagaa 
      atacgtcca taacaaaat gctggcggc ccgtaacgt 
      gggcatcac cagcgagac catccgcat gcccaaaac 
      ctccaccga ggcggaagt cctcgccaa ggtgcacca 
      gctcaacga ggaccccca ggtggacgg catcctggt 
      gcaactgcc ggtgccgga aggcatcga cgagcgcaa 
      ggtctgcaa cgccgtgga cccccgcaa ggacgtgga 
      cggcttcca catcctcaa cgtggggaa gctcaccct 
      caacatgga caccttcgt cccagccac ggccttggg 
      agtcatcga gctgatcaa gcgctcgaa tatcaagac 
      cttcggaaa aaatgtgct tatttgcgg gcgctcgaa 
      gaacgtggg gctgcctat ggcgatgct gttgcattc 
      ggacgcgcg gaatgaact ctcgggagg ggaggccac 
      cgtggtgct ctgccacag gaatacgcc tccagaaga 
      attggagtt tttcgcaaa aagggccga tattattat 
      aagtgctac gggggttgt ggggctcat taaaccgag 
      tatgattaa gccaggggc ttgtgttat tgatgtggg 
      cattacgag gattacgac cccggaagg caagagtaa 
      aattgttgg agatgttga ttttgaagg ggtgagcaa 
      aattgcggg gcatattac accggtccc ggggggcgt 
      tggccccat gactgtcgc catgctcat gcacaacac 
      ctttaaagc tgccatgga tcttgcgaa aagttcata 
      agtattata tttgtttgg acagtattg tcaaattat 
      ttatttttt aataaaaat ttaaatctg at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY