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Gene information
General information
TC005389 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13824616..13827317
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005389
    iBeetle screen iB_09413

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    odoriferous glands colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005389 RA
      MEVKTESPG DPNDRNGNV HKNTDLTLP HDFINNFAS 
      NNTTRKKIK KILIKRIPI LSWLPEYNC DCAVGDLVA 
      GITVGLTVI PQALAYSSV AGLPPQYGL YTSFLGCFV 
      YIFLGSCKD VAMGPTAIL ALLVHQVTE GKGPEYAIL 
      LCLLSGIVQ LLMGVLGLG FLIDFISGP VSSGFTSAA 
      ALIIVSSQI KDLLGIRAS GANFLDIWE GVVKDIGNT 
      KVWDCVLGI ACLVVLLLL RIISTVGTP ETGVPKWRT 
      ALRLIGISR NAILVIVCG AIGYYFTTL GETPFKVIG 
      YVPEGFPAI QPPRFTYTY NNTHENFLD MVTNLRSGI 
      IIVPLLGLL EDIAVCKAF ANGRPVDAT QELLAIGLC 
      NVANSFVQG FPGSGALAR SAVNNSSGV KTTLGGLYT 
      GVIVITALF FFTPYFQYI PKATLAAII IAAAIFMVE 
      IRVIQPMWR AKKSDLALA LITFVTCLV TRLEYGIST 
      GVVLNIIFI LYQAARPKI TIETMKSTQ GADYLLVTP 
      DRCLIFPSA DYVRNLVIK QSMRQKIPV VIDCSHIYG 
      ADYTAATVI ESLTKDFKT RQQPLFFYN LKSSVSSVF 
      VGLNLDYFL VYYNEDELD DMLKKWTEI CDKI
    CDS sequence
    • >TC005389 RA
      atggaggta aaaaccgag tctccggga gacccaaac 
      gaccgcaac ggcaacgtc cacaaaaac actgaccta 
      actctacca cacgatttt attaacaat ttcgcttca 
      aacaacaca accaggaaa aaaatcaaa aaaatccta 
      atcaagcga atcccaatt ttgagctgg ttgcctgaa 
      tataattgt gattgcgcc gttggggat ctggtggct 
      gggatcaca gtgggatta actgtaatc ccgcaagct 
      ttggcctat tccagcgtt gcaggactc ccaccccag 
      tatgggtta tacacctcg tttttgggc tgtttcgtt 
      tatattttt ctcgggagt tgtaaggac gtggccatg 
      ggccctacg gccattttg gcccttttg gtgcaccag 
      gtgaccgaa ggcaaaggg ccagagtat gcaattttg 
      ctgtgtttg ctcagtggg attgtgcaa cttttgatg 
      ggagttctg ggattagga ttcttgatt gatttcatt 
      tcgggacct gtgtcatca ggattcacc tcagctgct 
      gcccttatc attgtttcg tcacaaatc aaagacttg 
      ctagggatt agggcaagt ggggccaat tttttggac 
      atttgggaa ggtgttgtt aaagacata ggaaatacg 
      aaagtatgg gattgtgtt ttagggatt gcgtgtttg 
      gtagtcttg ctcttatta cggattata tcaactgtg 
      ggaaccccc gaaaccggc gttccaaaa tggcggacc 
      gccctccgc ctcatcgga atctcacgt aacgccatc 
      ttggtcatt gtttgcggc gctattggt tattatttc 
      accacatta ggggaaacc ccattcaag gtgattggt 
      tacgtcccg gagggattc cccgcaata caaccacca 
      cggttcacg tacacttac aacaacaca cacgagaat 
      ttcctcgac atggtcacc aacctacga tcggggatc 
      ataatcgtg cctttgttg ggcctcctt gaagacatt 
      gccgtttgc aaagccttc gcaaacgga cgaccggtt 
      gatgccaca caagaacta ctagcgatt ggtttatgc 
      aatgttgcc aactcattc gttcaggga ttccccgga 
      agtggggct ttggcgcgc agtgccgtt aataattca 
      agtggggtt aaaacaaca ctagggggg ctatacacg 
      ggcgttata gtcataaca gccctattt ttcttcact 
      ccttatttt caatacatt ccaaaagcg acactagcc 
      gccattatt atcgccgcc gccattttc atggtcgaa 
      attagggtc attcaaccc atgtggagg gccaaaaaa 
      agcgaccta gccctagcc cttatcact ttcgtcacc 
      tgtctggtg acaagacta gaatatggc atttcaaca 
      ggggttgta cttaacata attttcatt ttgtatcaa 
      gcggcaagg ccaaaaatt acaatcgaa accatgaaa 
      agcacgcaa ggagcagat tatttgctt gtgacgcct 
      gaccgatgc ttgattttt ccctcagct gattatgtc 
      cgaaatttg gtgattaag caatcgatg aggcaaaaa 
      attccggtt gttatcgac tgttcacac atttatggt 
      gctgattat acggcagct acagtcatt gagagttta 
      accaaagat ttcaaaacg cgacaacaa ccgctgttt 
      ttttacaat ctcaagtca agtgtgagc tcggttttt 
      gtcggccta aacctggac tattttcta gtctattac 
      aacgaagat gagttggat gatatgctc aagaagtgg 
      accgaaatc tgtgataaa atctag
    mRNA sequence
    • >TC005389 RA
      cgtcgaata acagacacc ttatcaaaa acccgccaa 
      ttatcatac aaaaacgac cacttttag taacaaaaa 
      cgcataatt atggaggta aaaaccgag tctccggga 
      gacccaaac gaccgcaac ggcaacgtc cacaaaaac 
      actgaccta actctacca cacgatttt attaacaat 
      ttcgcttca aacaacaca accaggaaa aaaatcaaa 
      aaaatccta atcaagcga atcccaatt ttgagctgg 
      ttgcctgaa tataattgt gattgcgcc gttggggat 
      ctggtggct gggatcaca gtgggatta actgtaatc 
      ccgcaagct ttggcctat tccagcgtt gcaggactc 
      ccaccccag tatgggtta tacacctcg tttttgggc 
      tgtttcgtt tatattttt ctcgggagt tgtaaggac 
      gtggccatg ggccctacg gccattttg gcccttttg 
      gtgcaccag gtgaccgaa ggcaaaggg ccagagtat 
      gcaattttg ctgtgtttg ctcagtggg attgtgcaa 
      cttttgatg ggagttctg ggattagga ttcttgatt 
      gatttcatt tcgggacct gtgtcatca ggattcacc 
      tcagctgct gcccttatc attgtttcg tcacaaatc 
      aaagacttg ctagggatt agggcaagt ggggccaat 
      tttttggac atttgggaa ggtgttgtt aaagacata 
      ggaaatacg aaagtatgg gattgtgtt ttagggatt 
      gcgtgtttg gtagtcttg ctcttatta cggattata 
      tcaactgtg ggaaccccc gaaaccggc gttccaaaa 
      tggcggacc gccctccgc ctcatcgga atctcacgt 
      aacgccatc ttggtcatt gtttgcggc gctattggt 
      tattatttc accacatta ggggaaacc ccattcaag 
      gtgattggt tacgtcccg gagggattc cccgcaata 
      caaccacca cggttcacg tacacttac aacaacaca 
      cacgagaat ttcctcgac atggtcacc aacctacga 
      tcggggatc ataatcgtg cctttgttg ggcctcctt 
      gaagacatt gccgtttgc aaagccttc gcaaacgga 
      cgaccggtt gatgccaca caagaacta ctagcgatt 
      ggtttatgc aatgttgcc aactcattc gttcaggga 
      ttccccgga agtggggct ttggcgcgc agtgccgtt 
      aataattca agtggggtt aaaacaaca ctagggggg 
      ctatacacg ggcgttata gtcataaca gccctattt 
      ttcttcact ccttatttt caatacatt ccaaaagcg 
      acactagcc gccattatt atcgccgcc gccattttc 
      atggtcgaa attagggtc attcaaccc atgtggagg 
      gccaaaaaa agcgaccta gccctagcc cttatcact 
      ttcgtcacc tgtctggtg acaagacta gaatatggc 
      atttcaaca ggggttgta cttaacata attttcatt 
      ttgtatcaa gcggcaagg ccaaaaatt acaatcgaa 
      accatgaaa agcacgcaa ggagcagat tatttgctt 
      gtgacgcct gaccgatgc ttgattttt ccctcagct 
      gattatgtc cgaaatttg gtgattaag caatcgatg 
      aggcaaaaa attccggtt gttatcgac tgttcacac 
      atttatggt gctgattat acggcagct acagtcatt 
      gagagttta accaaagat ttcaaaacg cgacaacaa 
      ccgctgttt ttttacaat ctcaagtca agtgtgagc 
      tcggttttt gtcggccta aacctggac tattttcta 
      gtctattac aacgaagat gagttggat gatatgctc 
      aagaagtgg accgaaatc tgtgataaa atctaggtg 
      taaaagcaa cacgtctgt gttttatag tacttttat 
      ttaattctg tataatgaa taaattatg gaaaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY