Please wait ...
Gene information
General information
TC005393 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13813673..13818147
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09411

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC005393 RA
    CDS sequence
    • >TC005393 RA
      atgcaagtc aatgtggct ggaattcgc cgtaattta 
      aaaaatttc gctcacaac tactccgat gctcagaag 
      aaagtccgc gaggcgact tcgaacgac ccatgggga 
      cccagcagt accataatg gcggaaatc gccgatctg 
      acatacaac gtggtggcc tttagcgaa ataatgcaa 
      atggtttgg aagcgatta aacgaccat ggaagaaac 
      tggcgacat gtgtacaaa gctctagtc cttttggag 
      tatttgata aagacgggg tcggaaaaa gtcggacag 
      cagtgcaag gagaacatt tttgccata cagacactg 
      aaggatttt cagcatttg gaagagggc aaggaccaa 
      ggacagaac gtccgagag aaggccaaa cagttggtg 
      aatttgtta aaggacgac gaaagactg aaaaatgaa 
      agagcgcgg gctcttaag gcgaaagag cgcttcgcg 
      caaagtgcc agcggattt gggagtgac ggtggtttg 
      gacacacct tgtagcccc aaatatccg gcgtttaga 
      ggcgactgg ggcaccagt cgtgacatg gaactgcct 
      cgagagata gaaaacgca agaccacaa acagccggc 
      gaagaagaa ctgcaattg cagttggcc ttggccatg 
      tcgagggag gaagccgaa caagaagaa caaaaaagg 
      aagtcggat gatgttaga ttacagttg gcattgagt 
      caaagccaa caagatttc aaagcaaac gaggagaag 
      aaacatcat agtcacatg gtggacctc ttggacgtt 
      aatctaggg gagacggag gcgccggtt gacccctgg 
      ggtatgcca cagccgcct cggagtcag caatcatcc 
      ccatggcaa ccacctgcc ctctcgtcg cccccgagc 
      agcacccca gcgccgggc gacccctgg cagccggtg 
      gcggcaatg cccccgaaa ccggaccca tggggcggc 
      gtaatgagc gccccctcg cccccgcag ccgccccaa 
      gccaagcac gacccttgg tcacccacc agtcaaagc 
      tcctctgac cttgacgac tttgacgta attacaaat 
      cgtaatcga acctcgccg aaatcaaca acaacaacg 
      aacggtaca cactcgaac gatccgttc gagctgaac 
      cttctgggt gaggctttg cccccgaca gcgctcagt 
      ccctcaacg ggggcgacg aaaaagaca ccgcagagt 
      ttcctcggc gagaattca gccttagtc aatttggat 
      aatttggtg acggctagt aaaccggcg aacgcccca 
      gctgctaac cccttctcg gagactcag ccccagcgc 
      cccgtgttc aacacgccg gcccccaag ccgacgata 
      aacgagatg aaacaggcc agttttgcg cagtttgcc 
      acagccccc agttcgacg gcctatggg ggcttcggg 
      gggcccgtg cagccggtg caggacccg tggacgccg 
      gcgcccagc gccaacttg ggggcgccg tggatgaaa 
      agcgaacaa gcaaatccg tttttgtct taa
    mRNA sequence
    • >TC005393 RA
      tcgcattaa ttccattag tctaaacaa atttgtaaa 
      aaattggcg agcaacgtc gcttaacct cattggtcc 
      ggtgtgaaa tgtcataat tcttgaaaa attgcgaat 
      ttgcccaac ttccgccac ttatcgggc gttttcccc 
      caaaactag ccgctcaaa accgaaatt tttgccaaa 
      aaaacacaa gaaattgca cagatcgtg tgtggtatt 
      cgaatcatc ttgaagaaa atacgccgc catgttgaa 
      tttctacgg tgaaaatga taaagtgta gtgtcatca 
      tttaggaca cgaaaacaa attaattta gctttaata 
      aatttgcag tgaccccgt aaatacttt gtaaaactc 
      ccagatttt cttgtgtgt gttaaaaca cgactgtgg 
      attttttaa gcgtagacg tatttttgg ttgtgcagt 
      tgtcaaaaa taggtgagt tttgtgtgt catttttgt 
      gtttattta ggtgcccaa ctatttatt tggtctttt 
      ttaggttct atcatgagt tttctagct ttgtgagct 
      ttgtataac gctttgtca gtgtttact ttacagaaa 
      agtgcaata tctcaagtg cctcgacat tagcaaaat 
      ttccattgt tttgcaagt aatttgagc ttttcattg 
      ctaccagct accaaattc cacccttct gtggacgta 
      attagtcaa ttagccaaa ttccttcgt gctttatgt 
      actgttttt ccaaaaact tgctaatca ctattgtta 
      gaaaaatcg cagataaga tacttgtta aatatttat 
      tgatcacac gagtgaaat cccacaagt ttctacatt 
      cctcacttg ttagcatgt gtaattagt tttggtttt 
      aattttttt ggcaacttt ttgcatgat tgttttcta 
      cataacgtt ttattaatg ttttcacag acggctgtt 
      ttaagtgga atctaacaa cgaaccaac taacaaaaa 
      aaaatgaga cgagggtcg cacgatatg caagtcaat 
      gtggctgga attcgccgt aatttaaaa aatttcgct 
      cacaactac tccgatgct cagaagaaa gtccgcgag 
      gcgacttcg aacgaccca tggggaccc agcagtacc 
      ataatggcg gaaatcgcc gatctgaca tacaacgtg 
      gtggccttt agcgaaata atgcaaatg gtttggaag 
      cgattaaac gaccatgga agaaactgg cgacatgtg 
      tacaaagct ctagtcctt ttggagtat ttgataaag 
      acggggtcg gaaaaagtc ggacagcag tgcaaggag 
      aacattttt gccatacag acactgaag gattttcag 
      catttggaa gagggcaag gaccaagga cagaacgtc 
      cgagagaag gccaaacag ttggtgaat ttgttaaag 
      gacgacgaa agactgaaa aatgaaaga gcgcgggct 
      cttaaggcg aaagagcgc ttcgcgcaa agtgccagc 
      ggatttggg agtgacggt ggtttggac acaccttgt 
      agccccaaa tatccggcg tttagaggc gactggggc 
      accagtcgt gacatggaa ctgcctcga gagatagaa 
      aacgcaaga ccacaaaca gccggcgaa gaagaactg 
      caattgcag ttggccttg gccatgtcg agggaggaa 
      gccgaacaa gaagaacaa aaaaggaag tcggatgat 
      gttagatta cagttggca ttgagtcaa agccaacaa 
      gatttcaaa gcaaacgag gagaagaaa catcatagt 
      cacatggtg gacctcttg gacgttaat ctaggggag 
      acggaggcg ccggttgac ccctggggt atgccacag 
      ccgcctcgg agtcagcaa tcatcccca tggcaacca 
      cctgccctc tcgtcgccc ccgagcagc accccagcg 
      ccgggcgac ccctggcag ccggtggcg gcaatgccc 
      ccgaaaccg gacccatgg ggcggcgta atgagcgcc 
      ccctcgccc ccgcagccg ccccaagcc aagcacgac 
      ccttggtca cccaccagt caaagctcc tctgacctt 
      gacgacttt gacgtaatt acaaatcgt aatcgaacc 
      tcgccgaaa tcaacaaca acaacgaac ggtacacac 
      tcgaacgat ccgttcgag ctgaacctt ctgggtgag 
      gctttgccc ccgacagcg ctcagtccc tcaacgggg 
      gcgacgaaa aagacaccg cagagtttc ctcggcgag 
      aattcagcc ttagtcaat ttggataat ttggtgacg 
      gctagtaaa ccggcgaac gccccagct gctaacccc 
      ttctcggag actcagccc cagcgcccc gtgttcaac 
      acgccggcc cccaagccg acgataaac gagatgaaa 
      caggccagt tttgcgcag tttgccaca gcccccagt 
      tcgacggcc tatgggggc ttcgggggg cccgtgcag 
      ccggtgcag gacccgtgg acgccggcg cccagcgcc 
      aacttgggg gcgccgtgg atgaaaagc gaacaagca 
      aatccgttt ttgtcttaa cgttggtaa aaggcacga 
      ttttttctt catacacca attgttgac acgtgtagc 
      ttttttacg ttgatctaa acaaatttg attttcgcg 
      cggaaatgt gatggattt attgctcat tttttgatt 
      gttattata caaagaaca tatctatat tttgattaa 
      ataaaattt aagatttta taacttgct tgcttcaag 
      ctttacttc agagatccg tcacttgga atagactga 
      taaatgtac agttttgta tgtcagtgt ttggttcgg 
      ttggtaaaa ctgcctcaa ttttatccg tttaccaaa 
      agtcttatt tattcaatg cctacgaca gtaattttc 
      taattttag tgtatattt ttaggttgt aaccagtat 
      ttttctgtc actttacat gttatgtat agaagaaat 
      tacgtaata tattgtatt taacttgag atatttgta 
      tatacttca aattattta ccgttcatg ttgagttat 
      gcaataata tattatttt taaactact tttaacaaa 
      catgtacat attaactaa ttttttagg ataggtttt 
      gtaacataa ggaggaaat aggagcgtt tatgaaggg 
      tttaatgga aatttatgt atcgtgata gctattttc 
      acttacatt cataaaaga aataaactg gagtggtgt 
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
    TFClass Survey

    Please help us to improve iBeetle-Base and get funded by participating in our survey.
    It will take only around 5 minutes. Thank you!

    OK, do not show again