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Gene information
General information
TC006729 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13445643..13449934
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006729
    iBeetle screen iB_09406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: best to be seen on the dorsal side
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006729 RA
      MSNNTAPDS WESQADSAS PNTSPSHNA DVTAKFSTL 
      NVNAVEFVP SFAKPAANA DEASPTSEA SPEHAPILN 
      GTSAESAER VEVKEPPPI PPADGDASP GDGGTWEDV 
      VVEDNDPLI TPEADDDEP SSEEAPIKI PKKKTVKEE 
      VLKDKKEHV NVVFIGHVD AGKSTIGGQ IMALTGMVD 
      KRTLEKYER EAREKSRES WYLSWALDT NQEERDKGK 
      TVEVGRAFF ETEKKHFTI LDAPGHKSF VPNMIGGAA 
      QADLAVLVI SARKGEFET GFDRGGQTR EHAMLAKTA 
      GVKYLVVLV NKMDDPTVN WDEARYNEC RDKILPYLK 
      KLGFNYNKD LFFLPCSGQ TGQGLKDQV DESICPWYR 
      GPAFIPFID DLPSLNRKI DGPFIMPIV DKYKDMGTV 
      LMGKVESGE CRKGQSLVI MPNRTPVTV DMLLSDDDE 
      VNRIVPGEN VKVKVKGVE EEDVSPGFV LCDAVNPIH 
      TGRVFDAQL VILEHKSII CAGYSAVMH IHCAAEEVT 
      VKALICLVD KKTGEKSKT RPRFVKQDQ VAIMRIECA 
      GVICLEPFK LFPQMGRFT LRDENKTIA IGKVLKLIE
    CDS sequence
    • >TC006729 RA
      atgtcgaat aacacagcc ccggacagt tgggaatct 
      caggccgac tccgcgtcg cccaacacc tcccccagt 
      cacaatgca gatgtgaca gcaaaattt tccacgttg 
      aacgtgaac gctgttgaa ttcgtgcct tcatttgca 
      aagcctgca gccaacgct gacgaggcc tcgccgacg 
      tccgaggcc tcccccgaa cacgccccc atcctcaac 
      ggaacgagc gctgagtcc gcagagcgg gtggaggtg 
      aaggagccc ccgcccatc ccccccgcg gacggggac 
      gcatccccc ggcgacggg ggcacctgg gaggacgtc 
      gtcgtcgag gacaacgac cccctcatc acgcccgag 
      gccgacgac gacgagccc tcctccgag gaggccccc 
      atcaaaata cccaagaag aagaccgtc aaggaggag 
      gtgctcaaa gacaagaag gaacacgtt aacgtggtt 
      tttatcggg catgtggac gcggggaaa tcgaccatt 
      ggggggcaa attatggcg ctgacgggc atggtggac 
      aagcgaacg ctggaaaag tacgaaagg gaagccagg 
      gagaaaagt cgggagtcg tggtacttg tcatgggcc 
      cttgacacg aaccaagaa gaaagggac aagggcaag 
      acggtggaa gtgggtcgg gcgtttttc gaaacggag 
      aagaaacac tttacgatt ttggacgcg ccagggcat 
      aagagtttt gttccgaat atgattggc ggagcggct 
      caggccgat ttggccgtt ttggttatt tcggccagg 
      aaaggggag tttgagacg gggtttgac cgaggtggt 
      cagacgaga gaacacgca atgttggcc aaaacggcc 
      ggtgttaaa tatttggtc gtgcttgtc aataaaatg 
      gacgatccg actgttaac tgggatgaa gctagatac 
      aacgaatgc agagacaaa attttaccc tatcttaaa 
      aaacttgga tttaactat aataaggat ctgttcttt 
      ttgccctgt tcgggacag acgggacaa gggctcaag 
      gatcaagtg gatgagtcg atctgtccc tggtataga 
      gggccggct ttcattccc ttcatcgac gacctgccg 
      tcactcaat cgaaaaatc gacggcccc ttcataatg 
      ccaatagta gacaaatac aaagacatg ggaacagtt 
      cttatgggg aaagtggaa tcgggtgaa tgtcgaaaa 
      ggacaaagt ctcgtcatt atgccaaac aggactccc 
      gtcactgtc gatatgttg ctctctgac gacgacgaa 
      gtcaatcgt atcgtaccc ggtgaaaat gtcaaagtt 
      aaagtaaaa ggtgttgaa gaagaagac gttagtcca 
      ggttttgta ctttgcgat gccgtcaat ccgatccac 
      accggtaga gtatttgat gctcagctt gtgattttg 
      gaacacaag tcgatcatt tgcgctggc tatagtgcc 
      gttatgcac attcattgc gccgccgaa gaagtcacc 
      gtcaaggcc ttaatttgt ctcgtggac aagaagact 
      ggtgaaaag agtaaaacg cggcccagg tttgtgaag 
      caagatcag gtagccata atgcgaata gaatgcgca 
      ggtgtgatt tgcctggag ccctttaaa ttgttccct 
      caaatgggc cgttttacg ctcagagat gaaaataaa 
      acaattgcc atcggaaag gtactgaag ttaatcgag 
      taa
    mRNA sequence
    • >TC006729 RA
      acaaagtaa ttagggcct tttcggata aaccgcgtc 
      acgtgtgga tattagaaa aagaatttt gtattttgg 
      gtcagtaac tggggacgg tggtggttg tgttattgt 
      taggggcga gttcttgtc atttgtgat gacagtcgg 
      ccattagtt tccggtggc agtgagtga acgcacgcg 
      gatatttag agcagctga aagctcgag ttttattaa 
      ttttccccg taaaagaac cgccccaag tgcacaaaa 
      tgtcgaata acacagccc cggacagtt gggaatctc 
      aggccgact ccgcgtcgc ccaacacct cccccagtc 
      acaatgcag atgtgacag caaaatttt ccacgttga 
      acgtgaacg ctgttgaat tcgtgcctt catttgcaa 
      agcctgcag ccaacgctg acgaggcct cgccgacgt 
      ccgaggcct cccccgaac acgccccca tcctcaacg 
      gaacgagcg ctgagtccg cagagcggg tggaggtga 
      aggagcccc cgcccatcc cccccgcgg acggggacg 
      catcccccg gcgacgggg gcacctggg aggacgtcg 
      tcgtcgagg acaacgacc ccctcatca cgcccgagg 
      ccgacgacg acgagccct cctccgagg aggccccca 
      tcaaaatac ccaagaaga agaccgtca aggaggagg 
      tgctcaaag acaagaagg aacacgtta acgtggttt 
      ttatcgggc atgtggacg cggggaaat cgaccattg 
      gggggcaaa ttatggcgc tgacgggca tggtggaca 
      agcgaacgc tggaaaagt acgaaaggg aagccaggg 
      agaaaagtc gggagtcgt ggtacttgt catgggccc 
      ttgacacga accaagaag aaagggaca agggcaaga 
      cggtggaag tgggtcggg cgtttttcg aaacggaga 
      agaaacact ttacgattt tggacgcgc cagggcata 
      agagttttg ttccgaata tgattggcg gagcggctc 
      aggccgatt tggccgttt tggttattt cggccagga 
      aaggggagt ttgagacgg ggtttgacc gaggtggtc 
      agacgagag aacacgcaa tgttggcca aaacggccg 
      gtgttaaat atttggtcg tgcttgtca ataaaatgg 
      acgatccga ctgttaact gggatgaag ctagataca 
      acgaatgca gagacaaaa ttttaccct atcttaaaa 
      aacttggat ttaactata ataaggatc tgttctttt 
      tgccctgtt cgggacaga cgggacaag ggctcaagg 
      atcaagtgg atgagtcga tctgtccct ggtatagag 
      ggccggctt tcattccct tcatcgacg acctgccgt 
      cactcaatc gaaaaatcg acggcccct tcataatgc 
      caatagtag acaaataca aagacatgg gaacagttc 
      ttatgggga aagtggaat cgggtgaat gtcgaaaag 
      gacaaagtc tcgtcatta tgccaaaca ggactcccg 
      tcactgtcg atatgttgc tctctgacg acgacgaag 
      tcaatcgta tcgtacccg gtgaaaatg tcaaagtta 
      aagtaaaag gtgttgaag aagaagacg ttagtccag 
      gttttgtac tttgcgatg ccgtcaatc cgatccaca 
      ccggtagag tatttgatg ctcagcttg tgattttgg 
      aacacaagt cgatcattt gcgctggct atagtgccg 
      ttatgcaca ttcattgcg ccgccgaag aagtcaccg 
      tcaaggcct taatttgtc tcgtggaca agaagactg 
      gtgaaaaga gtaaaacgc ggcccaggt ttgtgaagc 
      aagatcagg tagccataa tgcgaatag aatgcgcag 
      gtgtgattt gcctggagc cctttaaat tgttccctc 
      aaatgggcc gttttacgc tcagagatg aaaataaaa 
      caattgcca tcggaaagg tactgaagt taatcgagt 
      aaatcgtcg ctagattag attgtaaaa cttgacatt 
      ttaaccaac tcgcaatcg actggtgga catttttcc 
      gaggtgacc accacacaa tgttcattt tgtattgtc 
      ggtgaccac gaagagact ctagatcgg tgctacaaa 
      cagtagcgt gtgaaaatg atgaatttt ttaattaat 
      taattaatg ttttctttt ttgtatatt tagtctggt 
      gtaatggta taattttcc ttttagata tgttttgta 
      catttctaa ttttaattt attaaatta aggggtgta 
      ttattattt tagcgatcg aaattgtgt ggtgtacat 
      cattgttat taaaagagt tcttttgaa tcgatgtac 
      tccttttgt tcgacagcc tggtgcttt atttatatt 
      ttcgagacg agtgaaggt atttgacca cggcctatc 
      accacctga tgcgttgtt gactgagag aacaaagtg 
      tgcagtaaa taccttcaa gctcccggt tttatttac 
      aacacgcct tttcaaaga gttcgctct agctttgac 
      agttccatt gtaaataaa gagtttata agggtcacc 
      atgtggcca aacttaata aatttatgc ttatgttgt 
      attgcaaat tctttctgt taattaagt tgtgtttta 
      ctctttatt aataattat ataagtaac caagaaatg 
      ttgctatca attttatat attcaataa aatgtaaat 
      tggact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY