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Gene information
General information
TC006713 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13224301..13230165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006713
    iBeetle screen iB_09404

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006713 RA
      MAMEVMCDS SAWPAVAAS SSSTAATSS 
      SSNSTMVKT FQAYLPQCH RTYSCIHCR 
      AHLANHDEL ISKSFQGSQ GRAYLFNSV 
      VNVGCGPAE ERVLLTGLH AVADIYCEC 
      CKTTLGWKY EHAFESSQK YKEGKFIIE 
      LAHMIKENG WD
    CDS sequence
    • >TC006713 RA
      atggcgatg gaagtgatg tgtgacagc tccgcatgg 
      cctgccgtc gcagccagc agcagcagc accgcagcc 
      accagcagc agcagcaac agcaccatg gtgaagacg 
      ttccaggcc tacctgcca caatgccac cgcacctac 
      tcgtgcatc cactgccgg gcccatctc gccaatcac 
      gacgagctc atctcaaag tcattccaa ggcagccaa 
      ggccgggcg tatttgttc aattcagta gtgaatgtg 
      ggttgtggc cccgccgag gaaagagtt ctgttaacg 
      ggtcttcac gccgtcgcc gacatatac tgtgaatgt 
      tgcaagacg actttgggc tggaaatat gagcacgcc 
      ttcgagtcc agccagaag tacaaagag ggcaaattc 
      atcatcgaa ctagcacat atgatcaaa gaaaacgga 
      tgggactga
    mRNA sequence
    • >TC006713 RA
      gaatgaatg taataaagg atttatgac tcgaggctg 
      gacaaatac attcataaa aatggcgat ggaagtgat 
      gtgtgacag ctccgcatg gcctgccgt cgcagccag 
      cagcagcag caccgcagc caccagcag cagcagcaa 
      cagcaccat ggtgaagac gttccaggc ctacctgcc 
      acaatgcca ccgcaccta ctcgtgcat ccactgccg 
      ggcccatct cgccaatca cgacgagct catctcaaa 
      gtcattcca aggcagcca aggccgggc gtatttgtt 
      caattcagt agtgaatgt gggttgtgg ccccgccga 
      ggaaagagt tctgttaac gggtcttca cgccgtcgc 
      cgacatata ctgtgaatg ttgcaagac gactttggg 
      ctggaaata tgagcacgc cttcgagtc cagccagaa 
      gtacaaaga gggcaaatt catcatcga actagcaca 
      tatgatcaa agaaaacgg atgggactg acctttttg 
      aatctcgtt tcgagacga cgaaaatgc aaaaaaata 
      aaggactgt cacgaaaca atttctgtt ttctcgact 
      ggacttaaa ctcgggacg atttttcta ttttgccat 
      cgtgtcttt tgatttcga gcctttaca agccaaaat 
      gattttgag tttttggta gttggtgac gtcggacca 
      gggagttaa atgtcaatt ttcgggtga atgcgggcg 
      ggcactgcg actgttaaa ccttcaaat cttcacgac 
      aattcagct cttttgaaa gcattctgt ggtagatga 
      actgtgtct gtggtaatg aatctgtgg taacgaatg 
      attacgtgc tgtgcactg atgccgcta tttgggact 
      ttcgacggt ttgcgaaag acgggctca gtgcatatt 
      taggagtaa tttatttat atatttcgt gtatcacat 
      ggttttcta accgtaact agagttaag gtgttcgtt 
      ttgtttacc tgtattcag catgagcag gcaacaaag 
      ttgtaggcg gctaaattc acacttgaa aatgttcct 
      atttttcaa gacgatgct cacatgtgt tgatctcaa 
      ttcacgaca cctacaatt ctggctaaa ctgctcatc 
      gtaaatcta taaccacaa taaataaat tagttgtac 
      gcaatatgt aacacatat ttttgtatt cataatcgt 
      aatagttat ccctgtatg caaaaaaaa cggacaact 
      tacacaact tccaaagac tgatcccgc gcgaccagt 
      ctccggaag ttggatttt gtgatagtt tctttgtga 
      tatattctc cgtgctgaa gaattgccc tagactcct 
      ccattttcg tttgttgtt tttacgcaa ttcttcatc 
      attttctta agttagtgt gactctagt tacttttta 
      atcgaaaac ctaatagtt gtaagatca cgcggcaat 
      ctcacgttt cattcatac tgccagtag cgtagcggt 
      gtcatttat gtcatatat ttatggtaa cataatctt 
      ttgtattgt ttttagatt agttataca tttttttaa 
      gttcataag cctccattt tgtatggac ttgatttat 
      tatacaatt gggttatga aacttggtt ttgtgactc 
      atgcgaaat ttacataaa taaacgtaa ataaccatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY