Status
Please wait ...
Gene information
General information
TC006701 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13075436..13077846
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006701
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09402

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    mandible orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006701 RA
      MSIVGCQHL QSYKKTSKN KNTFKWIHA VFVVNSTSK 
      SLKTKLCNS RCRVCKKRG PFLHACLEC VFFGCHKHI 
      REHAKSQKH SMSMDLTYG QMHCNQCHD YIYDSEIDA 
      IANDNKMKS KTCKKRLFE IDTWNPTSD ERDYLRSKT 
      KKICITPDS TIGLRGLLN LGQTCFMNC IVQALMHTP 
      LLRDYFLTE EHNCKGMSG RCLVCEVSK LFQEFYNGT 
      RVPLALHEL LHLIWTHAR HLAGYEQQD AHEFFIATL 
      DVLHQHCLE TMPDLKPVN VGKNSRCPC IIDQIFTGG 
      LQSDVVCQK CNGVSTTID PISDFSLDL GPVTVGGRP 
      PTSLVDCLE RFTRAEHLG ENSKIMCSN CQSKQESTK 
      QLTMNTLPI VISFHLKRF QHMNEVKTH TKSKQKSLI 
      SSQMEKKIS TTISFPEML DMTPFMSRS KTDSPFPSD 
      NRYSLFAVI NHIGNSINV GHYTAYVRQ LQDYWYKCD 
      DHVITRATL KEVLDSEGY LLFYHKHVL DYD
    • >TC006701 RB
      MSIVGCQHL QSYKKTSKN KNTFKWIHA VFVVNSTSK 
      SLKTKLCNS RCRVCKKRG PFLHACLEC VFFGCHKHI 
      REHAKSQKH SMSMDLTYG QMHCNQCHD YIYDSEIDA 
      IANDNKMKS KTCKKRLFE IDTWNPTSD ERDYLRSKT 
      KKICITPDS TIGLRGLLN LGQTCFMNC IVQALMHTP 
      LLRDYFLTE EHNCKGMSG RCLVCEVSK LFQEFYNGT 
      RVPLALHEL LHLIWTHAR HLAGYEQQD AHEFFIATL 
      DVLHQHCLE TMPDLKPVN VGKNSRCPC IIDQIFTGG 
      LQSDVVCQK CNGVSTTID PISDFSLDL GPVTVGGRP 
      PTSLVDCLE RFTRAEHLG ENSKIMCSN CQSKQESTK 
      QLTMNTLPI VISFHLKRF QHMNEMEKK ISTTISFPE 
      MLDMTPFMS RSKTDSPFP SDNRYSLFA VINHIGNSI 
      NVGHYTAYV RQLQDYWYK CDDHVITRA TLKEVLDSE 
      GYLLFYHKH VLDYD
    CDS sequence
    • >TC006701 RA
      atgagcata gttgggtgt caacacttg cagtcttac 
      aagaaaacg agcaaaaac aagaacact ttcaagtgg 
      atccacgcc gtgttcgtg gtcaactcc acctcaaag 
      agcctcaaa accaagttg tgcaacagc cgctgtcgc 
      gtgtgcaaa aagcggggc cccttcctg catgcctgc 
      ctcgagtgt gtctttttc ggatgccac aagcacatc 
      cgggagcat gccaagagc cagaaacac tccatgtcc 
      atggacctg acctacggg cagatgcac tgcaaccaa 
      tgccatgac tacatctac gattccgaa atcgacgca 
      attgcgaac gataataaa atgaagtcg aagacttgc 
      aagaaacgg ctgtttgag atcgacacg tggaatcct 
      acgtctgat gagagggat tatttgagg agcaagacg 
      aagaagatt tgtattacg cccgattcg acgatcggg 
      ctaaggggg ctgcttaat ttaggtcag acgtgcttc 
      atgaattgt atagtgcaa gcactgatg cacaccccc 
      ttgttgcgc gactacttc ctcacagag gagcataac 
      tgcaagggg atgtcggga cggtgcctc gtttgcgag 
      gtctccaag cttttccag gagttttat aacggaact 
      cgcgtccct ttggccctc cacgagctc ttgcattta 
      atctggacg catgcccgg cacttagcc ggctatgag 
      cagcaagac gcccacgaa tttttcatc gcgactctg 
      gacgtgctc caccagcac tgtctcgag acaatgccc 
      gatttaaag ccggtgaat gtggggaaa aactcgcga 
      tgcccctgc attatcgac cagattttt accgggggg 
      ttgcaaagt gacgttgtt tgtcagaaa tgcaatggt 
      gtttctact acaattgat ccaatttcg gacttttcg 
      ttagatttg ggtccggtc acggttggc ggacggcca 
      cctacctca cttgtcgat tgtttagag aggttcaca 
      agggccgaa catttaggc gaaaattcg aaaatcatg 
      tgttcgaat tgccaatca aagcaggaa tcgacgaaa 
      caattgact atgaatacg cttccgatt gttatcagt 
      tttcattta aaacgcttt cagcacatg aacgaggta 
      aaaacccac acgaaatcc aaacaaaag tcactcatt 
      tcgtcgcag atggagaag aaaatctct actacgatt 
      tcgtttcct gaaatgcta gatatgacc ccgtttatg 
      agcaggtct aagacagac tcgcctttt ccctctgat 
      aacagatac tcgctcttt gccgtcatt aaccacatt 
      ggcaactcc ataaacgtg ggtcattac accgcttat 
      gtcagacag ttgcaagac tattggtac aaatgcgac 
      gatcacgtc atcacaaga gccactctc aaggaggtt 
      ctagactcg gaaggatat ttactcttt taccacaag 
      catgtccta gattacgac taa
    • >TC006701 RB
      atgagcata gttgggtgt caacacttg cagtcttac 
      aagaaaacg agcaaaaac aagaacact ttcaagtgg 
      atccacgcc gtgttcgtg gtcaactcc acctcaaag 
      agcctcaaa accaagttg tgcaacagc cgctgtcgc 
      gtgtgcaaa aagcggggc cccttcctg catgcctgc 
      ctcgagtgt gtctttttc ggatgccac aagcacatc 
      cgggagcat gccaagagc cagaaacac tccatgtcc 
      atggacctg acctacggg cagatgcac tgcaaccaa 
      tgccatgac tacatctac gattccgaa atcgacgca 
      attgcgaac gataataaa atgaagtcg aagacttgc 
      aagaaacgg ctgtttgag atcgacacg tggaatcct 
      acgtctgat gagagggat tatttgagg agcaagacg 
      aagaagatt tgtattacg cccgattcg acgatcggg 
      ctaaggggg ctgcttaat ttaggtcag acgtgcttc 
      atgaattgt atagtgcaa gcactgatg cacaccccc 
      ttgttgcgc gactacttc ctcacagag gagcataac 
      tgcaagggg atgtcggga cggtgcctc gtttgcgag 
      gtctccaag cttttccag gagttttat aacggaact 
      cgcgtccct ttggccctc cacgagctc ttgcattta 
      atctggacg catgcccgg cacttagcc ggctatgag 
      cagcaagac gcccacgaa tttttcatc gcgactctg 
      gacgtgctc caccagcac tgtctcgag acaatgccc 
      gatttaaag ccggtgaat gtggggaaa aactcgcga 
      tgcccctgc attatcgac cagattttt accgggggg 
      ttgcaaagt gacgttgtt tgtcagaaa tgcaatggt 
      gtttctact acaattgat ccaatttcg gacttttcg 
      ttagatttg ggtccggtc acggttggc ggacggcca 
      cctacctca cttgtcgat tgtttagag aggttcaca 
      agggccgaa catttaggc gaaaattcg aaaatcatg 
      tgttcgaat tgccaatca aagcaggaa tcgacgaaa 
      caattgact atgaatacg cttccgatt gttatcagt 
      tttcattta aaacgcttt cagcacatg aacgagatg 
      gagaagaaa atctctact acgatttcg tttcctgaa 
      atgctagat atgaccccg tttatgagc aggtctaag 
      acagactcg ccttttccc tctgataac agatactcg 
      ctctttgcc gtcattaac cacattggc aactccata 
      aacgtgggt cattacacc gcttatgtc agacagttg 
      caagactat tggtacaaa tgcgacgat cacgtcatc 
      acaagagcc actctcaag gaggttcta gactcggaa 
      ggatattta ctcttttac cacaagcat gtcctagat 
      tacgactaa
    mRNA sequence
    • >TC006701 RA
      ggcctaaaa ttgatttat taaattatt gcgtgaaac 
      ttatcgacc caaaataac attttcggg ttgtgggaa 
      tcacccgaa agccacagc aatcgtttc attgttgta 
      ttaaaatga tcaccgttt ctcgaatcg tgcttgtgg 
      gcatgtggc cgtaaatct cagtctgtt gtccaaagg 
      tggacacca gtgtcatat cgtgatttg tgtacaatt 
      tttaccaat tttttaaac tttttattg tgtgaatgg 
      aaacaatcg tttagtttt gtaaaaatc ggggcttgg 
      ggccaatag tttgaggcc acgactgta tctcgttgg 
      caacctcgt tgatgggca ataaatccc acacaaagc 
      cgcgttttt cgaaaaaaa tgagcatag ttgggtgtc 
      aacacttgc agtcttaca agaaaacga gcaaaaaca 
      agaacactt tcaagtgga tccacgccg tgttcgtgg 
      tcaactcca cctcaaaga gcctcaaaa ccaagttgt 
      gcaacagcc gctgtcgcg tgtgcaaaa agcggggcc 
      ccttcctgc atgcctgcc tcgagtgtg tctttttcg 
      gatgccaca agcacatcc gggagcatg ccaagagcc 
      agaaacact ccatgtcca tggacctga cctacgggc 
      agatgcact gcaaccaat gccatgact acatctacg 
      attccgaaa tcgacgcaa ttgcgaacg ataataaaa 
      tgaagtcga agacttgca agaaacggc tgtttgaga 
      tcgacacgt ggaatccta cgtctgatg agagggatt 
      atttgagga gcaagacga agaagattt gtattacgc 
      ccgattcga cgatcgggc taagggggc tgcttaatt 
      taggtcaga cgtgcttca tgaattgta tagtgcaag 
      cactgatgc acaccccct tgttgcgcg actacttcc 
      tcacagagg agcataact gcaagggga tgtcgggac 
      ggtgcctcg tttgcgagg tctccaagc ttttccagg 
      agttttata acggaactc gcgtccctt tggccctcc 
      acgagctct tgcatttaa tctggacgc atgcccggc 
      acttagccg gctatgagc agcaagacg cccacgaat 
      ttttcatcg cgactctgg acgtgctcc accagcact 
      gtctcgaga caatgcccg atttaaagc cggtgaatg 
      tggggaaaa actcgcgat gcccctgca ttatcgacc 
      agattttta ccggggggt tgcaaagtg acgttgttt 
      gtcagaaat gcaatggtg tttctacta caattgatc 
      caatttcgg acttttcgt tagatttgg gtccggtca 
      cggttggcg gacggccac ctacctcac ttgtcgatt 
      gtttagaga ggttcacaa gggccgaac atttaggcg 
      aaaattcga aaatcatgt gttcgaatt gccaatcaa 
      agcaggaat cgacgaaac aattgacta tgaatacgc 
      ttccgattg ttatcagtt ttcatttaa aacgctttc 
      agcacatga acgaggtaa aaacccaca cgaaatcca 
      aacaaaagt cactcattt cgtcgcaga tggagaaga 
      aaatctcta ctacgattt cgtttcctg aaatgctag 
      atatgaccc cgtttatga gcaggtcta agacagact 
      cgccttttc cctctgata acagatact cgctctttg 
      ccgtcatta accacattg gcaactcca taaacgtgg 
      gtcattaca ccgcttatg tcagacagt tgcaagact 
      attggtaca aatgcgacg atcacgtca tcacaagag 
      ccactctca aggaggttc tagactcgg aaggatatt 
      tactctttt accacaagc atgtcctag attacgact 
      aaacgattt tacctaatt taaacattt tatttattt 
      tattgtgac atcgattaa tttatcaag tgctaaatg 
      taataaatt ttacctcaa tag
    • >TC006701 RB
      ggcctaaaa ttgatttat taaattatt gcgtgaaac 
      ttatcgacc caaaataac attttcggg ttgtgggaa 
      tcacccgaa agccacagc aatcgtttc attgttgta 
      ttaaaatga tcaccgttt ctcgaatcg tgcttgtgg 
      gcatgtggc cgtaaatct cagtctgtt gtccaaagg 
      tggacacca gtgtcatat cgtgatttg tgtacaatt 
      tttaccaat tttttaaac tttttattg tgtgaatgg 
      aaacaatcg tttagtttt gtaaaaatc ggggcttgg 
      ggccaatag tttgaggcc acgactgta tctcgttgg 
      caacctcgt tgatgggca ataaatccc acacaaagc 
      cgcgttttt cgaaaaaaa tgagcatag ttgggtgtc 
      aacacttgc agtcttaca agaaaacga gcaaaaaca 
      agaacactt tcaagtgga tccacgccg tgttcgtgg 
      tcaactcca cctcaaaga gcctcaaaa ccaagttgt 
      gcaacagcc gctgtcgcg tgtgcaaaa agcggggcc 
      ccttcctgc atgcctgcc tcgagtgtg tctttttcg 
      gatgccaca agcacatcc gggagcatg ccaagagcc 
      agaaacact ccatgtcca tggacctga cctacgggc 
      agatgcact gcaaccaat gccatgact acatctacg 
      attccgaaa tcgacgcaa ttgcgaacg ataataaaa 
      tgaagtcga agacttgca agaaacggc tgtttgaga 
      tcgacacgt ggaatccta cgtctgatg agagggatt 
      atttgagga gcaagacga agaagattt gtattacgc 
      ccgattcga cgatcgggc taagggggc tgcttaatt 
      taggtcaga cgtgcttca tgaattgta tagtgcaag 
      cactgatgc acaccccct tgttgcgcg actacttcc 
      tcacagagg agcataact gcaagggga tgtcgggac 
      ggtgcctcg tttgcgagg tctccaagc ttttccagg 
      agttttata acggaactc gcgtccctt tggccctcc 
      acgagctct tgcatttaa tctggacgc atgcccggc 
      acttagccg gctatgagc agcaagacg cccacgaat 
      ttttcatcg cgactctgg acgtgctcc accagcact 
      gtctcgaga caatgcccg atttaaagc cggtgaatg 
      tggggaaaa actcgcgat gcccctgca ttatcgacc 
      agattttta ccggggggt tgcaaagtg acgttgttt 
      gtcagaaat gcaatggtg tttctacta caattgatc 
      caatttcgg acttttcgt tagatttgg gtccggtca 
      cggttggcg gacggccac ctacctcac ttgtcgatt 
      gtttagaga ggttcacaa gggccgaac atttaggcg 
      aaaattcga aaatcatgt gttcgaatt gccaatcaa 
      agcaggaat cgacgaaac aattgacta tgaatacgc 
      ttccgattg ttatcagtt ttcatttaa aacgctttc 
      agcacatga acgagatgg agaagaaaa tctctacta 
      cgatttcgt ttcctgaaa tgctagata tgaccccgt 
      ttatgagca ggtctaaga cagactcgc cttttccct 
      ctgataaca gatactcgc tctttgccg tcattaacc 
      acattggca actccataa acgtgggtc attacaccg 
      cttatgtca gacagttgc aagactatt ggtacaaat 
      gcgacgatc acgtcatca caagagcca ctctcaagg 
      aggttctag actcggaag gatatttac tcttttacc 
      acaagcatg tcctagatt acgactaaa cgattttac 
      ctaatttaa acattttat ttattttat tgtgacatc 
      gattaattt atcaagtgc taaatgtaa taaatttta 
      cctcaatag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY